scholarly journals Evaluation of the Stability of the Total Antioxidant Capacity, Polyphenol Contents, and Starch Hydrolase Inhibitory Activities of Kombucha Teas Using anIn VitroModel of Digestion

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Mindani I. Watawana ◽  
Nilakshi Jayawardena ◽  
Shakkya J. Ranasinghe ◽  
Viduranga Y. Waisundara
Keyword(s):  
Inhibitory Activity ◽  
Fermentation Process ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Different Types ◽  
Total Antioxidant ◽  
Phenolics Content ◽  
Inhibitory Activities ◽  
The Stability

The objective of this study was to evaluate and compare antioxidant and starch hydrolase inhibitory activity of three different types of Kombucha beverages prepared by three pellicles with different microbial compositions. The fermentation process was carried out for 7 days and the assessments of antioxidant and starch hydrolase inhibitory activities as well as tea phenolic compounds were carried out. These parameters were also evaluated after subjecting the final fermented samples to gastric and duodenal digestion in anin vitrodigestion model. The pH had a statistically significant decrease during the period of fermentation. The total phenolics content and antioxidant activities had increased during the fermentation process as well as when subjected to digestion. The starch hydrolase inhibitory activities also increased in a similar manner during the different phases. Theα-amylase andα-glucosidase inhibitory activities showed statistically significant increases(P<0.05)as the fermentation progressed, while an increase was observed after being subjected to pancreatic and duodenal digestion as well. All three types of tea showed a higherα-amylase inhibitory activity thanα-glucosidase inhibitory activity.

Antioxidant and starch hydrolase inhibitory properties of extracts of the antidiabetic herb Pterocarpus marsupium

2016 ◽  
Vol 63 (2) ◽  
pp. 124-133 ◽  
Author(s):  
Yian Hoon Lee ◽  
Candy Choo ◽  
Viduranga Y. Waisundara
Keyword(s):  
Total Phenolics ◽  
Water Extract ◽  
Antioxidant Compounds ◽  
Leaf Extracts ◽  
Pterocarpus Marsupium ◽  
Antidiabetic Treatment ◽  
Total Antioxidant ◽  
Therapeutic Properties ◽  
Phenolics Content ◽  
Inhibitory Activities

Pterocarpus marsupium is a popular spice incorporated into culinary preparations around the world, which is also used for its antidiabetic treatment in traditional medicine. The objective of the study was to evaluate the antioxidant and starch hydrolase inhibitory properties of Pterocarpus marsupium leaves. The total antioxidant capacity, total phenolics content, starch hydrolase inhibitory activities and superoxide dismutase mimetic (SODm) activity of various leaf extracts of Pterocarpus marsupium were studied. Extracts of dichloromethane (DCM), ethanol (EtOH), ethyl acetate (EtOAc), hexane (HEX), methanol (MeOH) and water were compared. The total phenolics contents of the extracts decreased in the order of water > EtOH > EtOAc > MeOH > DCM > HEX. The antioxidant and SODm activity values of the extracts decreased in the same order as the total phenolics contents, while the DPPH EC50 values increased in the reverse order. Pterosupin and pterostilbene had the highest content out of all the phenolic compounds quantified across all solvent extracts. The highest starch hydrolase inhibitory activities were observed in the water extract. Pterocarpus marsupium was observed to be a good source of antioxidant compounds and therapeutic properties.

scholarly journals Phenylpropanoids from Liparis nervosa and their in vitro antioxidant and α-glucosidase inhibitory activities

2021 ◽  
Author(s):  
liang liu ◽  
meijia zou ◽  
qimeng yin ◽  
zhenyang zhang ◽  
xianwen zhang
Keyword(s):  
Vitamin C ◽  
Medicinal Plant ◽  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
Antioxidant Activities ◽  
Active Constituents ◽  
Inhibitory Activities ◽  
Physicochemical Constants

Abstract Eleven phenylpropanoids were isolated from the whole grass of Liparis nervosa, an orchidaceous medicinal plant. Their structures were elucidated as (+)-Syringaresinol (1), (-)-Syringaresinol-4-O-β-D-glucopyranoside (2), Sinapaldehyde (3), Coniferyl aldehyde (4), Syringin (5), Sinapaldehye glucoside (6), 2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone (7), C-Veratroylglycol (8), 7S, 7′S, 8R, 8′R-icariol A2 (9), Erigeside 2 (10), and Methylsyringin (11) by comparing the spectroscopic data and physicochemical constants from the isolated compounds with the data reported in the literature. Compounds 1 and 9 were found to have potent in vitro antioxidant activities in the DPPH and ABTS assays, and their IC50 values were lower than those of vitamin C. More importantly, compound 9 had a strong α-glucosidase inhibitory activity with an IC50 value of 43.76 ± 2.03 µM, which was much lower than that of acarbose (IC50 = 273.12 ± 11.84 µM), indicating that compound 9 has the potential for the development of hypoglycemic drugs. In conclusion, the present study suggests that phenylpropanoids may be the additional representative type of active constituents in L. nervosa, which provides a new line of evidence to understand this medicinal plant.

scholarly journals Evaluation of the Antioxidant, Anti-Inflammatory and Cytoprotective Activities of Halophyte Extracts against Mycotoxin Intoxication

Toxins ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 312
Author(s):  
Nolwenn Hymery ◽  
Xavier Dauvergne ◽  
Halima Boussaden ◽  
Stéphane Cérantola ◽  
Dorothée Faugère ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Protective Action ◽  
Dry Weight ◽  
Cell Protection ◽  
Natural Habitats ◽  
Total Antioxidant ◽  
Mdbk Cells ◽  
Dpph Scavenging ◽  
T2 Toxin

Twelve halophyte species belonging to different families, widely represented along French Atlantic shoreline and commonly used in traditional medicine, were screened for protective activities against mycotoxins, in order to set out new promising sources of natural ingredients for feed applications. Selected halophytic species from diverse natural habitats were examined for their in vitro anti-mycotoxin activities, through viability evaluation of Madin-Darby Bovine Kidney (MDBK) and intestinal porcine enterocyte (IPEC-J2) cell lines. Besides, the in vitro antioxidant activities of plant extracts were assessed (total antioxidant and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging bioassays). Of the 12 species, Galium arenarium, Convolvulus soldanella and Eryngium campestre exhibited the most protective action on MDBK and IPEC-J2 cells against zearalenone (ZEN) or T2 toxin contamination (restoring about 75% of cell viability at 10 μg·mL−1) without inflammation response. They also had strong antioxidant capacities (Inhibitory concentration of 50% (IC50) < 100 μg·mL−1 for DPPH radical and total antioxidant capacity (TAC) of 100 to 200 mg Ascorbic Acid Equivalent (AAE)·g−1 Dry Weight), suggesting that cell protection against intoxication involves antioxidant action. A bio-guided study showed that fractions of G. arenarium extract protect MDBK cells against T2 or ZEN toxicity and several major compounds like chlorogenic acid and asperuloside could be involved in this protective effect. Overall, our results show that the halophytes G. arenarium, C. soldanella and E. campestre should be considered further as new sources of ingredients for livestock feed with protective action against mycotoxin intoxication.

scholarly journals Health benefits of new symbiotic “NAR”

2014 ◽  
Vol 2 ◽  
Author(s):  
Saule Saduakhasova ◽  
Almagul Kushugulova ◽  
Samat Kozhakhmetov ◽  
Gulnara Shakhabayeva ◽  
Adil Supiyev ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Reductase ◽  
Inflammatory Mediators ◽  
Antioxidant Activities ◽  
Reductase Activity ◽  
Protein A ◽  
Intact Cells ◽  
Total Antioxidant

Introduction: The immune-modulatory effects of synbiotics and their ability to reduce free radical levels may be useful for functional food that is able to be active throughout whole period of colonization of the gastrointestinal tract.The aim of the present study was to investigate the immune-modulatory and antioxidant effects of the synbiotic product "NАR," a probiotic beverage.Methods: The presence of IL-2, IL-4, IL-6, IL-8, IL-10, αTNF, γIFN, Ig A, Ig M, and Ig E was studied in vitro using a solid immunosorbent analysis. The total antioxidant activities of superoxide dismutase and glutathione reductase were determined by a spectrophotometry using the Sigma-Aldrich sets.Results: Studies of the immune-modulatory properties of the synbiotic product NAR showed 1.7 fold increase of γINF levels (p<0.01) in blood after consumption of the synbiotic product “NAR” in comparison to control values, whereas the concentrations of IL-4 and Ig E decreased 2.0 times (treatment: 9.3; control: 18.7; p<0.01) and 1.3 times (p<0.1), respectively. The consumption of the synbiotic product “NAR” caused an increase in the proportion of γINF/IL 4 (treatment: 15.4; control: 4.4; p<0.01), which indicates a reduction in functional activity of Th2-type lymphocytes in comparison with the function of Th1 cells.Our study showed a high level of the total antioxidant activity of the synbiotic product (67.4 mmol/ml). The antioxidant activity of the intact cells of consortium (15.3 mM/ml), which was the basis for the preparation of the symbiotic product, is several times lower than the activity observed in the symbiotic samples.Expression of SOD is one of the mechanisms of antioxidant stress radicals inactivation by bacteria. The analysis identified a superoxide dismutase activity of synbiotic product (1.42 U/mg protein). A glutathione reductase activity of the synbiotic product was elevated (0.06 U/ml). Conclusion: The majority of the inflammatory mediators found in the blood after the consumption of symbiotic product NAR were inflammatory mediators that activate a cellular component of the resistance. Moreover, the symbiotic product has a high antioxidant activity. 

scholarly journals EVALUATION OF DEHYDRATED MUKIA MADERASPATANA LEAVES AS A POTENT SOURCE OF DIETARY ANTIOXIDANTS

2019 ◽  
Vol 7 (6) ◽  
pp. 27-36
Author(s):  
D. Amirtham ◽  
J. Aswini Nivedida ◽  
K. Dhivya ◽  
S. Ganapathy ◽  
C. Indurani
Keyword(s):  
Phenolic Content ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Antioxidant Property ◽  
Leafy Vegetables ◽  
Total Phenolic ◽  
Green Leafy Vegetables ◽  
Total Antioxidant ◽  
Indigenous Plant

Green leafy vegetables are the most underexploited class of vegetables despite high nutritional value. The current study has been focused on the evaluation of anti-oxidant status of fresh and dehydrated under- utilized green leafy vegetable namely Mukia maderaspatana (L.) (Family: Cucurbitaceae), an indigenous plant; traditionally it is used as an ingredient of various cocktail preparations for the management of severe inflammatory disorders in Indian system of medicine. The total phenolic content (TPC), total flavonoid content (TFC) and total antioxidant activities were evaluated for the ethanolic extract of leaves to assess the in vitro antioxidant activities. The results showed that there exist a linear correlation between polyphenol content and antioxidant property. The ethanolic extract of dehydrated Mukia leaves showed the highest phenolic content (269.34 ± 0.78mg GAE/g), and total antioxidant activity (543±46 µmol Trolox/100 g). HPTLC analysis has revealed the presence of significant quantity of Quercitin (26.52%), an important flavonoid of tremendous antioxidant, anticancer and ant inflammatory properties in both the fresh and dehydrated leaves which might be the chief bioactive principle in Mukia.

scholarly journals Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites

2021 ◽  
Vol 5 (3) ◽  
pp. e202101237
Author(s):  
Kutub Ashraf ◽  
Shahin Tajeri ◽  
Christophe-Sébastien Arnold ◽  
Nadia Amanzougaghene ◽  
Jean-François Franetich ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Plasmodium Falciparum ◽  
Inhibitory Activity ◽  
Parasite Species ◽  
Artemisia Annua ◽  
Combination Therapies ◽  
Lead Compounds ◽  
Low Concentrations ◽  
Inhibitory Activities

Artemisinin-based combination therapies (ACT) are the frontline treatments against malaria worldwide. Recently the use of traditional infusions from Artemisia annua (from which artemisinin is obtained) or Artemisia afra (lacking artemisinin) has been controversially advocated. Such unregulated plant-based remedies are strongly discouraged as they might constitute sub-optimal therapies and promote drug resistance. Here, we conducted the first comparative study of the anti-malarial effects of both plant infusions in vitro against the asexual erythrocytic stages of Plasmodium falciparum and the pre-erythrocytic (i.e., liver) stages of various Plasmodium species. Low concentrations of either infusion accounted for significant inhibitory activities across every parasite species and stage studied. We show that these antiplasmodial effects were essentially artemisinin-independent and were additionally monitored by observations of the parasite apicoplast and mitochondrion. In particular, the infusions significantly incapacitated sporozoites, and for Plasmodium vivax and P. cynomolgi, disrupted the hypnozoites. This provides the first indication that compounds other than 8-aminoquinolines could be effective antimalarials against relapsing parasites. These observations advocate for further screening to uncover urgently needed novel antimalarial lead compounds.

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