scholarly journals Analysis of Bovine Serum Albumin Ligands fromPuerariae flosUsing Ultrafiltration Combined with HPLC-MS

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Ping Tang ◽  
Shihui Si ◽  
Liangliang Liu
Keyword(s):  
Natural Products ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
High Performance ◽  
Rapid Screening ◽  
Active Compounds ◽  
Fundamental Parameters ◽  
Isolation Techniques ◽  
Puerariae Flos

Rapid screening techniques for identification of active compounds from natural products are important not only for clarification of the therapeutic material basis, but also for supplying suitable chemical markers for quality control. In the present study, ultrafiltration combined with high performance liquid chromatography-mass spectrometry (HPLC-MS) was developed and conducted to screen and identify bovine serum albumin (BSA) bound ligands fromPuerariae flos. Fundamental parameters affecting the screening like incubation time, BSA concentration, pH, and temperature were studied and optimized. Under the optimum conditions, nine active compounds were identified by UV and MS data. The results indicated that this method was able to screen and identify BSA bound ligands form natural products without the need of preparative isolation techniques. Moreover, the method has more effective with easier operation procedures.

scholarly journals Antioxidant Activities of Centella asiatica Extract-loaded Bovine Serum Albumin Nanoparticles in Simulated Gastrointestinal System Study

2020 ◽  
Vol 141 ◽  
pp. 03001
Author(s):  
Kittiya Kesornbuakao ◽  
Patteera Chanapongpisan ◽  
Malinee Sriariyanun ◽  
Ir. Lindayani ◽  
Patchanee Yasurin
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Crude Extract ◽  
Bovine Serum ◽  
Antioxidant Activities ◽  
Centella Asiatica ◽  
Gastrointestinal System ◽  
Physical Parameters ◽  
Active Compounds ◽  
Albumin Nanoparticles

Bioavailability of active compounds extracted from herbs is generally limited to be adsorbed or expressed to target organisms due to several physical and chemical factors. Nanoparticle encapsulation techniques was developed to carry bioactive macromolecules of Centella asiatica (Buabok) in the form of C. asiatica Extract-loaded Bovine Serum Albumin Nanoparticles (CBNPs) to improve bioavailability. In this study, the antioxidant activities of CBNPs and C. asiatica crude extract were evaluated by using DPPH radical scavenging assay in the simulated gastrointestinal system, including mastication, stomach, duodenum, and ileum conditions to provide the environment which similar to in vivo system in terms of chemical and physical parameters. CBNPs were prepared by mixing of C. asiatica crude extract and BSA at different ratio of 1:2, 1:3, and 1:4. The results showed that the highest antioxidant activity of CBNP was observed when the ratio of crude extract and BSA at 1:2 was used at pH 2.0 or in the simulated stomach condition. The denaturation or unfolding of BSA in the simulated stomach occurred when pH was low could lead to the release of active compounds at certain area in gastrointestinal system.

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