scholarly journals Tiao He Yi Wei Granule, a Traditional Chinese Medicine, against Ethanol-Induced Gastric Ulcer in Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Jinfu Yao
Keyword(s):  
Gastric Ulcer ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Group Treatment ◽  
Underlying Mechanism ◽  
Ethanol Ingestion ◽  
Model Group ◽  
Sod Activity ◽  
Mda Content ◽  
Necrosis Factor

Tiao He Yi Wei granule (DHYW), a traditional Chinese medicine, has been used for the treatment of gastric ulcer in clinical setting. The purpose of the present study was to investigate the possible effect of DHYW and explore the underlying mechanism against ethanol-induced gastric ulcer in mice. The model of ethanol-induced gastric ulcer in mice was induced by ethanol (0.2 mL/kg). Administration of DHYW at the doses of 250, 500 mg/kg body weight prior to the ethanol ingestion could effectively protect the stomach from ulceration. The gastric lesions were significantly ameliorated in the DHYW group compared with that in the model group. Treatment with DHYW markedly decreased the levels of interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α(TNF-α). In addition, DHYW treatment elevated myeloperoxidase (MPO) level in stomach, increased superoxide dismutase (SOD) activity, and decreased malonaldehyde (MDA) content in serum and stomach compared with those in the model group. DHYW significantly inhibited NF-κB pathway expressions in the gastric mucosa ulcer group. Taken together, DHYW exerted a gastroprotective effect against gastric ulceration and the underlying mechanism might be associated with NF-κB pathway.

scholarly journals Gastric ulcer and traditional chinese medicine

2021 ◽  
Vol 6 (2) ◽  
Author(s):  
Huan Xu ◽  
Huan Yao ◽  
Zhong Jiang ◽  
Xiao Wu ◽  
Zixian Chen ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine

scholarly journals Inhibition of JAK3/STAT3 Activation in Rats Uterine Leiomyoma by formula of Taohong Siwu Decoction: A Randomized Controlled Trial

2021 ◽  
Author(s):  
Li Luo ◽  
Shasha Shi ◽  
Li Zhang ◽  
Xun Xu ◽  
Fu Peng ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Uterine Leiomyoma ◽  
Immune Escape ◽  
Drug Group ◽  
Control Group ◽  
Model Group ◽  
Indoleamine 2 ◽  
Taohong Siwu Decoction

Abstract Background: Traditional Chinese medicine (TCM) formula of Taohong Siwu decoction (THSW) is often used in traditional Chinese medicine for the treatment of uterine leiomyoma (UL). The effectiveness of THSW for the treatment of UL has been confirmed in previous studies. At present, there are few relevant mechanism studies. The purpose of this study is to explore the efficacy and mechanism of THSW in the treatment of uterine leiomyoma. Methods: A Sprague Dawley (SD) rat model of UL was established via estrogen and progesterone load combined with external stimulation for 5 weeks. We randomly assigned adult female non-pregnant rats into six groups: a control group, a UL model group, and a positive drug group, THSW(18g/ml) group, THSW(9g/ml) group, THSW(4.5g/ml) group. THSW group and positive drug group were treated with THSW medicinal solutions and gongliuxiao capsule medicinal solutions respectively after daily modeling for 3 weeks. Histological analyses, Polymerase Chain Reaction and western blotting were performed to evaluate the effect of THSW on UL and elucidate its mechanism of action. Results: The uterus morphology of the model group showed significantly more swelling than that of the control group, and pathological of changes of rats were obvious in the model group. Compared with model group, the pathological changes of UL were relieved in the THSW group. Our data showed that the treatment of rats with THSW significantly reduced the expression of cytotoxic T lymphocyte associated antign 4 (CTLA4) and indoleamine 2, 3-dioxygenase 1 (INDO). The phosphorylation of JAK3 and STAT3 which are the main signal transduction molecule of cytokines and growth factorswere were also inhibited. Conclusions: Our results suggest that THSW is effective in the prevention and treatment of UL in rats, and THSW may exert its functions by inhibiting the activation of Tumor-related signal transduction pathway (JAK3/STAT3), immune escape, proliferation of tumor cell and improving apoptosis.

scholarly journals Precocious Puberty and the Lin28/Let7 Pathway: The Therapeutic Effect of the Nourishing “Yin” and Purging “Fire” Traditional Chinese Medicine Mixture in a Rat Model

2018 ◽  
Vol 2018 ◽  
pp. 1-15 ◽  
Author(s):  
Yuanyuan He ◽  
Xinghui Han ◽  
Wen Sun ◽  
Jian Yu ◽  
Amin Tamadon
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Precocious Puberty ◽  
Serum Levels ◽  
Female Rats ◽  
Control Group ◽  
Vaginal Opening ◽  
Model Group ◽  
Nourishing Yin ◽  
The Relationship

The present study aims to investigate the effects of the nourishing “Yin” and purging “Fire” Traditional Chinese Medicine (TCM) herb mixture on precocious puberty and TCM may act through hypothalamic Lin28/let7 pathway expression in the precocious puberty model rats. Meanwhile, to confirm the relationship between Lin28/let7 pathway and puberty by overexpressionLin28a,in the first part of this study, female rats were randomly allocated into untreated controls, the precocious puberty (PP) model group, the PP control group, and the PP + TCM group. Rats on postnatal day 5 were injected danazol to establish the PP model. From days 15 to 35, the rats in the TCM group were given the TCM twice daily. Vaginal opening, sex-related hormones, and body and reproductive organ weights were measured, and the expressions of hypothalamicLin28aandLin28bmRNA andlet7aandlet7bmiRNA were detected. In addition, in the second part, the effects of overexpression ofLin28aon the vaginal opening time were evaluated. In the two parts of the study, we found that, at the onset of puberty, a decrease in ovary weight, an increase in the serum levels of luteinizing hormone and progesterone, and increased expression levels of hypothalamicLin28bmRNA were observed in the PP + TCM group compared to the PP model group. The vaginal opening time was significantly delayed upon overexpression ofLin28a. Above all, the mechanism by which the TCM treats precocious puberty is thus likely to be associated with inhibition of the hypothalamic Lin28/let7 signaling pathway and our findings provide in-depth insight into the relationship between the overexpression ofLin28agene in the hypothalamus and the onset of puberty.

A Network Pharmacology Approach to Explore the Underlying Mechanism of Tufuling Qiwei Tangsan in Treating Psoriasis

2021 ◽  
Vol 16 ◽  
Author(s):  
Xiaolei Ma ◽  
Yinan Lu ◽  
Yang Lu ◽  
Zhili Pei
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Mechanism Of Action ◽  
Enrichment Analysis ◽  
Underlying Mechanism ◽  
Mapk Signaling ◽  
Binding Activity ◽  
Network Pharmacology ◽  
Active Components ◽  
Active Compounds

Background: Tufuling Qiwei Tangsan (TQTS) is a commonly used Mongolian medicine preparation against psoriasis in China. However, its mechanism of action and molecular targets for the treatment of psoriasis is still unclear. Network pharmacology can reveal the synergistic mechanism of drugs at the molecular, target and pathway levels, and is suitable for the complex study of traditional Chinese medicine formulations. However, it is rarely involved in the application of Mongolian medicine with the same holistic concept of traditional Chinese medicine. Method: In this paper, the active compounds of TQTS were collected and their targets were identified. Psoriasis-related targets were obtained by analyzing the differential expressed genes between psoriasis patients and healthy individuals. Then, the network concerning the interactions of potential targets of TQTS with well-known psoriasis-related targets was built. The core targets were selected according to topological parameters. And the enrichment analysis was carried out to explore the mechanism of action of TQTS. Moreover, molecular docking was performed to study the interaction between the selected ligands and receptors related to psoriasis. Result and Conclusion: Eighty-five active compounds of TQTS were screened, with corresponding 270 targets, and 313 differentially expressed genes were identified. Additionally, enrichment analysis showed that the targets of TQTS for treating psoriasis were mainly concentrated in multiple biological processes, including apoptosis, growth factor response,etc., and related pathways including PI3K-Akt and MAPK signaling pathway, and so on. Genes such as NFKB1, TP53 and MAPK1 are the key genes in the gene pathway network of TQTS against psoriasis. The 4 main active components of TQTS have certain binding activity with 13 potential targets, and the stability of interaction with AKT1 is the best, which indicate the potential mechanism of TQTS on psoriasis.

scholarly journals Hydroxysafflor Yellow A Attenuates Hydrogen Peroxide-Induced Oxidative Damage on Human Umbilical Vein Endothelial Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Yuefeng Xie ◽  
Yan Guo ◽  
ShiDong Cao ◽  
Miaomiao Xue ◽  
ZhaoYue Fan ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Chinese Medicine ◽  
Cardiovascular Diseases ◽  
Traditional Chinese Medicine ◽  
Oxidative Damage ◽  
Umbilical Vein ◽  
Hydroxysafflor Yellow A ◽  
Sod Activity ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Oxidative stress of endothelial cells is thought to be a principal cause that induces many cardiovascular diseases. Hydroxysafflor yellow A (HSYA) is a major active component in traditional Chinese medicine safflower and has been used to cure ischemic cardiovascular diseases in China for many years. This study aims to investigate whether HSYA has a repairing effect on oxidative damage of human umbilical vein endothelial cells (HUVECs) induced by H2O2 and to provide a theoretical basis for the clinical treatment of cardiovascular diseases related to traditional Chinese medicine. Based on the establishment of an H2O2-induced HUVEC oxidative injury model, the cell viability and proliferation rate were measured by the MTT assay and EdU staining. The intracellular GSH/GSSG ratio and SOD activity were determined by kits. The ROS level was detected by flow cytometry. And the BAX, Bcl-2, PTEN, and AKT expressions were evaluated with western blotting methods. The results showed that HSYA treatment significantly attenuated the H2O2-induced HUVEC cell damage, increased the intracellular GSH/GSSG ratio and unit SOD activity also, and decreased the intracellular ROS levels. Furthermore, HSYA increased the expressions of AKT and Bcl-2 proteins and inhibited the expressions of BAX and PTEN proteins. These suggest that HSYA exerts repair effects on H2O2-induced oxidative damage in HUVECs, and the mechanisms may be related to the influence of BAX/Bcl-2 expression and AKT/PTEN signal pathway expression.

scholarly journals Inactivated Lactobacillus promotes protection against myocardial ischemia–reperfusion injury through NF-κB pathway

2017 ◽  
Vol 37 (6) ◽  
Author(s):  
Ni Wang ◽  
Genhong Song ◽  
Yang Yang ◽  
Weiwei Yuan ◽  
Ming Qi
Keyword(s):  
Myocardial Infarction ◽  
Myocardial Ischemia ◽  
Ischemia Reperfusion ◽  
Group Model ◽  
Content Increase ◽  
Model Group ◽  
Sod Activity ◽  
Apoptosis Rate ◽  
Mda Content ◽  
Myocardial Ischemia Reperfusion

Although restoration of blood flow to an ischemic organ is essential to prevent irreversible cellular injury, reperfusion may augment tissue injury in excess of that produced by ischemia alone. So this experiment was designed to study the protective effects and mechanism of inactivated Lactobacillus (Lac) on myocardial ischemia–reperfusion (I–R) injury (MIRI). MIRI rat models were established by ligation of left anterior descending coronary artery for ~30 min and then, reperfusion for 120 min and divided into control group, model group, and Lac (106, 107, and 108 cfu/kg) groups. At the end of the test, the creatine kinase (CK) activity, lactate dehydrogenase (LDH) activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were assayed by corresponding kits. The heart was obtained from rats and the myocardial infarction area was determined by TTC staining and myocardial endothelial cell apoptosis rate was determined by Tunel kit. Besides, A20, IκB, nuclear factor (NF)-κB, and nitric oxide (NO) synthase (NOS) were also assayed by Western blot. When compared with model group, Lac obviously reduces MIRI in the rat by reducing myocardial infarction area and the apoptosis rate of endothelial cells; reduce the serum CK, LDH, and MDA content; increase the serum SOD activity; and suppress NF-κB signaling and NOS expression in the myocardial tissues. Lac pretreatment can inhibit lipid peroxidation and effectively improve MIRI caused by oxygen free radical through inhibiting NF-κB signaling.

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