Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester:In VitroAssays

BioMed Research International ◽

10.1155/2015/627471 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Abuzar Elnager ◽

Rosline Hassan ◽

Zamzuri Idris ◽

Zulkifli Mustafa ◽

Nadiah Wan-Arfah ◽

...

Keyword(s):

Caffeic Acid ◽

Fibrinolytic Activity ◽

Thrombolytic Agent ◽

Caffeic Acid Phenethyl Ester ◽

Blood Clots ◽

Dependent Activity ◽

Potential Alternative ◽

Dose Dependent ◽

Dose Dependent Effect

Background. Caffeic acid phenethyl ester (CAPE) has been reported to possess time-dependent fibrinolytic activity byin vitroassay. This study is aimed at investigating fibrinolytic dose-dependent activity of CAPE usingin vitroassays.Methods. Standardized human whole blood (WB) clots were incubated in either blank controls or different concentrations of CAPE (3.75, 7.50, 15.00, 22.50, and 30.00 mM). After 3 hours, D-dimer (DD) levels and WB clot weights were measured for each concentration. Thromboelastography (TEG) parameters were recorded following CAPE incubation, and fibrin morphology was examined under a confocal microscope.Results. Overall, mean DD (μg/mL) levels were significantly different across samples incubated with different CAPE concentrations, and the median pre- and postincubation WB clot weights (grams) were significantly decreased for each CAPE concentration. Fibrin removal was observed microscopically and indicated dose-dependent effects. Based on the TEG test, the Ly30 fibrinolytic parameter was significantly different between samples incubated with two different CAPE concentrations (15.0 and 22.50 mM). The 50% effective dose (ED50) of CAPE (based on DD) was 1.99 mg/mL.Conclusions. This study suggests that CAPE possesses fibrinolytic activity followingin vitroincubation and that it has dose-dependent activities. Therefore, further investigation into CAPE as a potential alternative thrombolytic agent should be conducted.

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Effect of caffeic acid phenethyl ester on gastric acid secretion in vitro

European Journal of Pharmacology ◽

10.1016/j.ejphar.2005.08.032 ◽

2005 ◽

Vol 521 (1-3) ◽

pp. 139-143 ◽

Cited By ~ 5

Author(s):

Francesca Borrelli ◽

Inmaculada Posadas ◽

Raffaele Capasso ◽

Gabriella Aviello ◽

Valeria Ascione ◽

...

Keyword(s):

Acid Secretion ◽

Gastric Acid Secretion ◽

Gastric Acid ◽

Caffeic Acid ◽

Caffeic Acid Phenethyl Ester

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Formulation development of folic acid conjugated PLGA nanoparticles for improved cytotoxicity of Caffeic acid phenethyl ester

Pharmaceutical Nanotechnology ◽

10.2174/2211738509666210111160528 ◽

2021 ◽

Vol 09 ◽

Author(s):

Harshad S Kapare ◽

Sathiyanarayanan L ◽

Arulmozhi S ◽

Kakasaheb Mahadik

Keyword(s):

Folic Acid ◽

Drug Release ◽

Caffeic Acid ◽

Caffeic Acid Phenethyl Ester ◽

Therapeutic Effects ◽

Formulation Development ◽

Sustained Drug Release ◽

Active Constituent

Background: Honey bee propolis is one of the natural product reported in various traditional systems of medicines including Ayurveda. Caffeic acid phenethyl ester (CAPE) is an active constituent of propolis which is well known for its anticancer potential. The therapeutic effects of CAPE are restricted owing to its less aqueous solubility and low bioavailability. Objective: In this study CAPE loaded folic acid conjugated nanoparticle system (CLFPN) was investigated to enhance solubility, achieve sustained drug release and improved cytotoxicity of CAPE. Methods: Formulation development, characterization and optimization were carried out by design of experiment approach. In vitro and in vivo cytotoxicity study was carried out for optimized formulations. Results: Developed nanoparticles showed particle size and encapsulation efficiency of 170 ± 2 - 195 ± 3 nm and 75.66 ± 1.52 - 78.80 ± 1.25 % respectively. Optimized formulation CLFPN showed sustained drug release over a period of 42 h. GI50 concentration was decreased by 46.09% for formulation as compared to CAPE in MCF-7 cells indicating targeting effect of CLFPN. An improved in vitro cytotoxic effect was reflected in in-vivo Daltons Ascites Lymphoma model by reducing tumor cells count. Conclusion: The desired nanoparticle characteristic with improved in vivo and in vitro cytotoxicity was shown by developed formulation. Thus it can be further investigated for biomedical applications.

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TITANIUM NANOPARTICLES CONJUGATED WITH STREPTOKINASE AS A MODIFIED THROMBOLYTIC AGENT

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2020v12i1.36824 ◽

2020 ◽

pp. 18-25

Author(s):

HAYDER H. ABED ◽

ESTABRAQ AR. ALWASITI ◽

AMIR T. TAWFEEQ

Keyword(s):

Thrombolytic Agent ◽

Blood Clot ◽

Control Group ◽

Thrombolytic Activity ◽

Protein Assay ◽

Blood Clots ◽

Ft Ir ◽

Titanium Nanoparticles ◽

D Dimer

Objective: Blood clots are the main cause of death worldwide by stroke and myocardial infarction. Streptokinase a thrombolytic agent that is used in the treatment of circulatory disorders. Methods: Titanium Nanoparticles was supplied from Changsha Santech Co. Its characterized were studied using (FT-IR, XRD, AFM, FE-SEM). Streptokinase at concentration 0.1 mg/ml was conjugated with Titanium nanoparticles using PH equal to 5.2 with continuous stirring. Formation of Streptokinase loading Titanium nanoparticles confirmed using FT-IR, Ninhydrine’s test and Bradford protein assay. Physicochemical Properties were studied in vitro. Thrombolytic activity in vitro was determined using d–dimer indicator and weight of blood clot after treatment as indicators of thrombolytic activity. Results: Titanium nanoparticles show particle size at range 31 nm. The thrombolytic activity of streptokinase loading Titanium nanoparticles shows significant value in d-dimer and weight of blood clot compared with the control group and non-significant compared with an equivalent amount of streptokinase alone. Conclusion: Titanium nanoparticles conjugated with streptokinase show high thrombolytic activity against blood clots in vitro.

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Antifungal and Anti-Biofilm Effects of Caffeic Acid Phenethyl Ester on Different Candida Species

Antibiotics ◽

10.3390/antibiotics10111359 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1359

Author(s):

Ibrahim Alfarrayeh ◽

Edit Pollák ◽

Árpád Czéh ◽

András Vida ◽

Sourav Das ◽

...

Keyword(s):

Cell Death ◽

Candida Species ◽

Caffeic Acid Phenethyl Ester ◽

Dependent Manner ◽

Cell Shrinkage ◽

Nuclear Condensation ◽

Cell Death Response ◽

Dose Dependent ◽

Dose Dependent Effect ◽

Strain Dependent

This study investigated the effect of CAPE on planktonic growth, biofilm-forming abilities, mature biofilms, and cell death of C. albicans, C. tropicalis, C. glabrata, and C. parapsilosis strains. Our results showed a strain- and dose-dependent effect of CAPE on Candida, and the MIC values were between 12.5 and 100 µg/mL. Similarly, the MBIC values of CAPE ranging between 50 and 100 µg/mL highlighted the inhibition of the biofilm-forming abilities in a dose-dependent manner, as well. However, CAPE showed a weak to moderate biofilm eradication ability (19-49%) on different Candida strains mature biofilms. Both caspase-dependent and caspase-independent apoptosis after CAPE treatment were observed in certain tested Candida strains. Our study has displayed typical apoptotic hallmarks of CAPE-induced chromatin margination, nuclear blebs, nuclear condensation, plasma membrane detachment, enlarged lysosomes, cytoplasm fragmentation, cell wall distortion, whole-cell shrinkage, and necrosis. In conclusion, CAPE has a concentration and strain-dependent inhibitory activity on viability, biofilm formation ability, and cell death response in the different Candida species.

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Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/6067172 ◽

2017 ◽

Vol 2017 ◽

pp. 1-14 ◽

Cited By ~ 5

Author(s):

Jéssica Rebouças-Silva ◽

Fabiana S. Celes ◽

Jonilson Berlink Lima ◽

Hernane S. Barud ◽

Camila I. de Oliveira ◽

...

Keyword(s):

Cutaneous Leishmaniasis ◽

Biological Effects ◽

Parasite Burden ◽

Infectious Agents ◽

Cell Toxicity ◽

Propolis Extract ◽

Inflammatory Profile ◽

Dose Dependent ◽

Dose Dependent Effect

Treatments based on antimonials to cutaneous leishmaniasis (CL) entail a range of toxic side effects. Propolis, a natural compound widely used in traditional medical applications, exhibits a range of biological effects, including activity against infectious agents. The aim of this study was to test the potential leishmanicidal effects of different propolis extracts against Leishmania (Viannia) braziliensis promastigotes and intracellular amastigotes in vitro. Stationary-phase L. (V) braziliensis promastigotes were incubated with medium alone or treated with dry, alcoholic, or glycolic propolis extract (10, 50, or 100 μg/mL) for 96 h. Our data showed that all extracts exhibited a dose-dependent effect on the viability of L. (V) braziliensis promastigotes, while controlling the parasite burden inside infected macrophages. Dry propolis extract significantly modified the inflammatory profile of murine macrophages by downmodulating TGF-β and IL-10 production, while upmodulating TNF-α. All three types of propolis extract were found to reduce nitric oxide and superoxide levels in activated L. braziliensis-infected macrophages. Altogether, our results showed that propolis extracts exhibited a leishmanicidal effect against both stages of L. (V) braziliensis. The low cell toxicity and efficient microbicidal effect of alcoholic or glycolic propolis extracts make them candidates to an additive treatment for cutaneous leishmaniasis.

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In Vivo and In Vitro Antıneoplastic Actions of Caffeic Acid Phenethyl Ester (CAPE): Therapeutic Perspectives

Nutrition and Cancer ◽

10.1080/01635581.2013.776693 ◽

2013 ◽

Vol 65 (4) ◽

pp. 515-526 ◽

Cited By ~ 56

Author(s):

Sumeyya Akyol ◽

Gulfer Ozturk ◽

Zeynep Ginis ◽

Ferah Armutcu ◽

M. Ramazan Yigitoglu ◽

...

Keyword(s):

Caffeic Acid ◽

Caffeic Acid Phenethyl Ester

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Caffeic acid phenethyl ester: Inhibition of metastatic cell behaviours via voltage-gated sodium channel in human breast cancer in vitro

The International Journal of Biochemistry & Cell Biology ◽

10.1016/j.biocel.2015.12.012 ◽

2016 ◽

Vol 71 ◽

pp. 111-118 ◽

Cited By ~ 12

Author(s):

Scott P. Fraser ◽

Faye Hemsley ◽

Mustafa B.A. Djamgoz

Keyword(s):

Breast Cancer ◽

Sodium Channel ◽

Caffeic Acid ◽

Human Breast Cancer ◽

Caffeic Acid Phenethyl Ester ◽

Human Breast ◽

Voltage Gated Sodium Channel ◽

Voltage Gated ◽

Metastatic Cell

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Low concentrations of caffeic acid phenethyl ester stimulate osteogenesis in vitro

Tissue and Cell ◽

10.1016/j.tice.2021.101618 ◽

2021 ◽

pp. 101618

Author(s):

Paulo Henrique Neves Santos ◽

Hebert Lacerda Silva ◽

Elizabeth Ferreira Martinez ◽

Júlio César Joly ◽

Ana Paula Dias Demasi ◽

...

Keyword(s):

Caffeic Acid ◽

Caffeic Acid Phenethyl Ester ◽

Low Concentrations ◽

Osteogenesis In Vitro

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Potential cytoprotection: antioxidant defence by caffeic acid phenethyl ester against free radical-induced damage of lipids, DNA, and proteins

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y08-029 ◽

2008 ◽

Vol 86 (5) ◽

pp. 279-287 ◽

Cited By ~ 31

Author(s):

Ting Wang ◽

Lixiang Chen ◽

Weimin Wu ◽

Yuan Long ◽

Rui Wang

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Caffeic Acid ◽

Erythrocyte Membrane ◽

Polyacrylamide Gel Electrophoresis ◽

Caffeic Acid Phenethyl Ester ◽

Water Soluble ◽

Pbr322 Dna ◽

Acid Esters

Oxidative stress is considered to be a major cause of cellular injuries in a variety of chronic health problems, such as carcinogenesis and neurodegenerative disorders. Caffeic acid phenethyl ester (CAPE), derived from the propolis of honeybee hives, possesses a variety of biological and pharmacological properties including antioxidant and anticancer activity. In the present study, we focused on the diverse antioxidative functionalities of CAPE and its related polyphenolic acid esters on cellular macromolecules in vitro. The effects on human erythrocyte membrane ghost lipid peroxidation, plasmid pBR322 DNA, and protein damage initiated by the water-soluble initiator 2,2′-azobis(2-amidinopropane) hydrochloride (AAPH) and hydrogen peroxide (H2O2) were monitored by formation of hydroperoxides and by DNA nicking assay, single-cell alkaline electrophoresis, and SDS-polyacrylamide gel electrophoresis. Our results showed that CAPE and its related polyphenolic acid esters elicited remarkable inhibitory effects on erythrocyte membrane lipid peroxidation, cellular DNA strand breakage, and protein fragmentation. The results suggest that CAPE is a potent exogenous cytoprotective and antigenotoxic agent against cell oxidative damage that could be used as a template for designing novel drugs to combat diseases induced by oxidative stress components, such as various types of cancer.

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