scholarly journals Protective Effect of Ethanol Extracts ofHericium erinaceuson Alloxan-Induced Diabetic Neuropathic Pain in Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Zhang Yi ◽  
Yang Shao-long ◽  
Wang Ai-hong ◽  
Sun Zhi-chun ◽  
Zhuo Ya-fen ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Total Antioxidant Status ◽  
Treated Group ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Diabetic Neuropathic Pain ◽  
Laboratory Rats ◽  
Withdrawal Threshold ◽  
Total Antioxidant ◽  
Ethanol Extracts

We investigated the effects ofHericium erinaceus(HEE) on alloxan induced diabetic neuropathic pain in laboratory rats. Alloxan induced diabetic rats were administered orally HEE. After 6 weeks of treatments, treatment with HEE 40 mg/kg in diabetic animals showed significant increase in pain threshold and paw withdrawal threshold and significant decrease in serum glucose and urine glucose. We also observed a significant increase in lactate dehydrogenase (LDH), Lipid peroxidation (LPO), glutathione peroxidase (GPx) activity, glutathione reductase (GR) activity, catalase (CAT) activity, Na+K+ATPase activity, and glutathione S transferase (GST) activity along with significant decreased levels of glutathione (GSH) content in diabetic rats. The total antioxidant status (TAOS) in the HEE-treated groups was significantly lower than that in the alloxan-treated group. HEE can offer pain relief in diabetic neuropathic pain. The improvement in diabetic state after HEE treatment along with the antioxidant activity could be the probable way by which it had alleviated diabetic neuropathy.

scholarly journals Protective Effect ofAgaricus brasiliensison STZ-Induced Diabetic Neuropathic Pain in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Weifeng Ji ◽  
Haiying Huang ◽  
Ji Chao ◽  
Wuchao Lu ◽  
Jianyou Guo
Keyword(s):  
Body Weight ◽  
Neuropathic Pain ◽  
Neuroprotective Effect ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Dependent Manner ◽  
Diabetic Neuropathic Pain ◽  
Laboratory Rats ◽  
Withdrawal Threshold ◽  
Behavioral Parameters

Objective. The present investigation examined the neuroprotective effect ofAgaricus brasiliensis(AbS) against STZ-induced diabetic neuropathic pain in laboratory rats. STZ-induced diabetic rats were administered orally with AbS. Body weight, serum glucose, and behavioral parameters were measured before and at the end of the experiment to see the effect of AbS on these parameters. After 6 weeks of treatments, all animals were sacrificed to study various biochemical parameters. Treatment with AbS 80 mg/kg in diabetic animals showed significant increase in body weight, pain threshold, and paw withdrawal threshold and significant decrease in serum glucose, LPO and NO level, Na-K-ATPase level, and TNF-αand IL-1βlevel as compared to vehicle treated diabetic animals in dose and time dependent manner. AbS can offer pain relief in PDN. This may be of potential benefit in clinical practice for the management of diabetic neuropathy.

scholarly journals N-Acetylcysteine Attenuates Hyperalgesia in Rats with Diabetic Neuropathic Pain: Role of Oxidative Stress and Inflammatory Mediators and CXCR4

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Sisi Li ◽  
Xuying Li ◽  
Xiangbin Xie ◽  
Xiao Wei ◽  
Cong Yu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Neuropathic Pain ◽  
Prefrontal Cortex ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Diabetic Neuropathic Pain ◽  
Withdrawal Threshold ◽  
Sprague Dawley Rats ◽  
Therapeutic Value ◽  
Tnf Α

Objectives. CXCR4 plays critical roles in the development of diabetic neuropathic pain (DNP) in rats, and its mechanism is unknown. This study was aimed at evaluating the potential therapeutic value of the antioxidant N-acetylcysteine (NAC) against DNP in rats and how CXCR4 participates in the formation of DNP. Methods. Control or streptozotocin- (STZ-) induced diabetic Sprague-Dawley rats received vehicle or NAC for four weeks starting one week after STZ injection. Von Frey and Hargreaves Apparatus were used to analyze the behavioral changes of mechanical allodynia and heat hyperalgesia. CXCR4, p-CXCR4, interleukin- (IL-) 6, and tumor necrosis factor- (TNF-) α in the spinal cord and the prefrontal cortex were detected by western blotting. Plasma IL-6, TNF-α, superoxide dismutase- (SOD-) 1, SOD-2, and lipid peroxidation products malondialdehyde (MDA) and 15-F2t-Isoprostane were detected by ELISA. Results. The values of paw withdrawal threshold (PWT) and paw withdrawal latencies (PWL) were reduced in diabetic rats compared to control rats that were concomitant with significant increases of CXCR4, p-CXCR4, IL-6, and TNF-α protein expressions in the spinal cord and prefrontal cortex. The treatment with NAC decreased the IL-6 and TNF-α protein expression and further increased CXCR4 and p-CXCR4 in the spinal cord and the cortex of diabetic rats that were accompanied with enhancement of PWT and PWL. NAC also significantly attenuated or reverted the increases of plasma IL-6, TNF-α, SOD-1, SOD-2, MDA, and 15-F2t-Isoprostane in diabetic rats. Conclusion. It is concluded that NAC treatment could effectively alleviate DNP and that induction of CXCR4 and p-CXCR4 may represent a mechanism whereby NAC attenuates DNP.

scholarly journals Garlic Increases Antioxidant Levels in Diabetic and Hypertensive Rats Determined by a Modified Peroxidase Method

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Hana Drobiova ◽  
Martha Thomson ◽  
Khaled Al-Qattan ◽  
Riitta Peltonen-Shalaby ◽  
Zainab Al-Amin ◽  
...  
Keyword(s):  
Antioxidant Status ◽  
Correlation Coefficients ◽  
Total Antioxidant Status ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Total Serum ◽  
Hypertensive Rats ◽  
Modified Method ◽  
Before And After ◽  
Total Antioxidant

Oxidative damage by free radicals has been implicated in the pathogenesis of vascular disease in diabetes and hypertension. In the present study, the total antioxidant status in diabetic and hypertensive rats before and after treatment with garlic (Allium sativum) was determined. The total serum antioxidants were measured by a modified method reported earlier by Miller and coworkers. The reproducibility of the assay was confirmed by determining standard curves for the known antioxidants: trolox (a stable analog of vitamin E), glutathione and vitamin C with interassay correlation coefficients (R2,n= 10 in triplicate) of 0.9984, 0.9768 and 0.987, respectively, confirming the reliability and reproducibility of the assay. This assay was then used to determine total serum antioxidant levels of streptozotocin-induced diabetic rats and two-kidney one-clip hypertensive rats both before and after 3 weeks of treatment with an aqueous extract of garlic (500 mg/kg IP daily). The serum antioxidant levels of rats after 3 weeks of treatment were significantly higher (P< .001) than the pretreatment levels in both diabetic and hypertensive rats. The increased serum antioxidant levels were paralleled by a decrease in serum glucose in the garlic-treated diabetic rats and lowered systolic blood pressure in the garlic-treated hypertensive rats. We conclude from our study that (i) total antioxidants can be measured by a simple, reproducible, reliable assay and (ii) the total antioxidant status can be significantly improved by treatment with garlic.

scholarly journals EGCG Prevents the Loss of Pontine Noradrenergic Neurons Induced by Diabetes: A Role in Diabetic Neuropathic Pain

2012 ◽  
Vol 18 (S5) ◽  
pp. 5-6 ◽  
Author(s):  
Carla Morgado ◽  
João Silva ◽  
André Miranda ◽  
Patrícia Pereira-Terra ◽  
Diogo Raposo ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Neuropathic Pain ◽  
Diabetic Rats ◽  
Pain Modulation ◽  
Diabetic Patients ◽  
Spontaneous Pain ◽  
Noradrenergic Neurons ◽  
Diabetic Neuropathic Pain ◽  
Descending Pain Modulation ◽  
Pain Responses

Diabetes is a major health problem with an alarming increasing prevalence, and is the most frequent cause of neuropathy worldwide. Neuropathy affects 50–60% of diabetic patients, being a major life-quality impairment for a quarter of these patients. Diabetic neuropathic pain (DNP) is characterized by spontaneous pain, mechanical hyperalgesia and tactile allodynia and is accompanied by functional and neurochemical changes at the peripheral nerves, spinal cord and supraspinal pain control areas. Regarding the effects of diabetic neuropathy in the central somatossensory system, it was shown that streptozotocin (STZ)-diabetic rats present spontaneous hyperactivity and hyperexcitability of spinal nociceptive neurons, which may be subserving the exacerbated pain responses. The spinal functional changes and pain may be due to increased peripheral input(2), changes in spinal nociceptive modulatory mechanisms and altered supraspinal descending pain modulation. Noradrenergic descending pain modulation seems to be impaired since STZ-diabetic rats present decreased numbers of noradrenergic neurons at the A5 and A7 pontine cell groups, along with lower levels of noradrenaline at the spinal cord and higher behavioral responses to pain. This is consistent with the strong noradrenergic projection from A5 and A7 neurons to the spinal dorsal horn and the modulation of nociceptive transmission by local release of noradrenaline. The mechanisms underlying the decrease in noradrenergic neurons in the brainstem during diabetes remain unclear. Our recent findings that diabetes induces oxidative stress damage in neurons from those areas, lead us to hypothesize that it may contribute to their loss. Thereafter, with the present study we aimed to evaluate the effects of Epigallocathechin Gallate (EGCG), a potent antioxidant present in green tea, on spinal noradrenaline levels, on A5 and A7 noradrenergic neurons and on behavioral pain responses of STZ-diabetic rats.

scholarly journals Hypersensitivity of Spinothalamic Tract Neurons Associated With Diabetic Neuropathic Pain in Rats

2002 ◽  
Vol 87 (6) ◽  
pp. 2726-2733 ◽  
Author(s):  
Shao-Rui Chen ◽  
Hui-Lin Pan
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Thermal Hyperalgesia ◽  
Diabetic Rats ◽  
Projection Neurons ◽  
Diabetic Neuropathic Pain ◽  
Spinothalamic Tract ◽  
Functional Changes ◽  
Spinal Dorsal

Diabetic neuropathic pain is often considered to be caused by peripheral neuropathy. The involvement of the CNS in this pathological condition has not been well documented. Development of hypersensitivity of spinal dorsal horn neurons is involved in neuropathic pain induced by traumatic nerve injury. In the present study, we determined the functional changes of identified spinothalamic tract (STT) neurons and their correlation to diabetic neuropathic pain. Diabetes was induced in rats by intraperitoneal injection of streptozotocin. Hyperalgesia and allodynia were assessed by the withdrawal responses to pressure, radiant heat, and von Frey filaments applied to the hindpaw. Single-unit activity of STT neurons was recorded from the lumbar spinal cord in anesthetized rats. The responses of STT neurons to mechanical and thermal stimuli and the sensitivity to intravenous morphine were determined in diabetic and normal rats. In 12 diabetic rats, mechanical allodynia and hyperalgesia, but not thermal hyperalgesia, developed within 2 wk after streptozotocin injection and lasted for ≥7 wk. Compared to the 32 STT neurons recorded in normal animals, the 37 STT neurons in diabetic rats displayed a higher spontaneous discharge activity and enlarged receptive fields. Also, the STT neurons in diabetic rats exhibited lower thresholds and augmented responses to mechanical stimulation. Intravenous injection of 2.5 mg/kg of morphine suppressed significantly the responses of STT neurons to noxious stimuli in 12 nondiabetic rats. However, such an inhibitory effect of morphine on the evoked response of STT neurons was diminished in 14 diabetic animals. This electrophysiological study provides new information that development of hypersensitivity of spinal dorsal horn projection neurons may be closely related to neuropathic pain symptoms caused by diabetes. Furthermore, the attenuated inhibitory effects of morphine on evoked responses of STT neurons in diabetes likely accounts for its reduced analgesic efficacy in this clinical form of neuropathic pain.

scholarly journals Remarkable Anticancer Activity ofTeucrium poliumon Hepatocellular Carcinogenic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Ariyo Movahedi ◽  
Rusliza Basir ◽  
Asmah Rahmat ◽  
Mohammad Charaffedine ◽  
Fauziah Othman
Keyword(s):  
Biochemical Markers ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Liver Lesion ◽  
Treated Group ◽  
Cancer Development ◽  
Herbal Therapy ◽  
Total Antioxidant ◽  
Serum Biochemical ◽  
Teucrium Polium

The term cancer has been concomitant with despair, agony, and dreadful death. Like many other diseases, herbal therapy has been used to prevent or suppress cancer. The present study investigated the capability of the decoction ofTeucrium poliumL. from Lamiaceae family to protect liver cells against hepatocellular carcinoma in carcinogenesis-induced animal model. After 28 weeks of treatment with decoction ofTeucrium poliumL., serum biochemical markers including ALT, AST, AFP, GGT, ALP, HCY, TNF-α,α2MG, and CBG have been regulated auspiciously. Total antioxidant status also has been increased intensely. Liver lesion score in treated group was lessened and glucocorticoid activity has been intensified significantly. In conclusion,Teucrium poliumL. decoction might inhibit or suppress liver cancer development.

scholarly journals Inhibition of phosphorylated calcium/calmodulin-dependent protein kinase IIα relieves streptozotocin-induced diabetic neuropathic pain through regulation of P2X3 receptor in dorsal root ganglia

2022 ◽  
Author(s):  
Xiao-fen He ◽  
Yu-rong Kang ◽  
Xue-yu Fei ◽  
Lu-hang Chen ◽  
Xiang Li ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Fasting Blood Glucose ◽  
Thermal Hyperalgesia ◽  
Underlying Mechanism ◽  
Diabetic Rats ◽  
Diabetic Neuropathic Pain ◽  
Calcium Calmodulin Dependent ◽  
Patients With Diabetes

Abstract  Diabetic neuropathic pain (DNP) is frequent among patients with diabetes. We previously showed that P2X3 upregulation in dorsal root ganglia (DRG) plays a role in streptozotocin (STZ)-induced DNP but the underlying mechanism is unclear. Here, a rat model of DNP was established by a single injection of STZ (65 mg/kg). Fasting blood glucose was significantly elevated from the 1st to 3rd week. Paw withdrawal thresholds (PWTs) and paw withdrawal latencies (PWLs) in diabetic rats significantly reduced from the 2nd to 3rd week. Western blot analysis revealed that elevated p-CaMKIIα levels in the DRG of DNP rats were accompanied by pain-associated behaviors while CaMKIIα levels were unchanged. Immunofluorescence revealed significant increase in the proportion of p-CaMKIIα immune positive DRG neurons (stained with NeuN) in the 2nd and 3rd week and p-CaMKIIα was co-expressed with P2X3 in DNP rats. KN93, a CaMKII antagonist, significantly reduce mechanical hyperalgesia and thermal hyperalgesia and these effects varied dose-dependently, and suppressed p-CaMKIIα and P2X3 upregulation in the DRGs of DNP rats. These results revealed that the p-CaMKIIα upregulation in DRG is involved in DNP, which possibly mediated P2X3 upregulation, indicating CaMKIIα may be an effective pharmacological target for DNP management.

scholarly journals Hypoglycemic effect of Irvingia gabonensis (Aubry-Lacomate Ex. Ororke), Baill in Type 2 Diabetic Long-Evans Rats

2012 ◽  
Vol 11 (1) ◽  
pp. 19-24 ◽  
Author(s):  
M S Hossain ◽  
S Sokeng ◽  
M Shoeb ◽  
K Hasan ◽  
M Mosihuzzaman ◽  
...  
Keyword(s):  
Glucose Level ◽  
Serum Insulin ◽  
Liver Glycogen ◽  
Treated Group ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Bush Mango ◽  
Irvingia Gabonensis ◽  
Type 2 Diabetic

Irvingia gabonensis (Aubry-Lacomate Ex. Ororke), Baill (African wild mango/bush mango) seeds are   widely used in cooking as a sauce in Cameroon and in most parts of tropical Africa for the treatment of a number of ailments. In this study normal rat food was incorporated with I. gabonensis seed powder (10%) and oil free seed powder (5%) and their chronic effects on streptozotocin induced Type 2 diabetic rats were studied. Oral consumption of food incorporated with seed powder significantly reduced serum glucose level on the 28th day (p<0.01) which was   comparable with glibenclamide treated group. Food with oil free seed powder showed 24% fall in glucose level on the 28th day. Fasting serum insulin increased significantly (p<0.001) in glibenclamide and oil free seed powder treated (p<0.008) groups. No effect was observed in the seed powder treated group. Liver glycogen content increased in the glibenclamide treated group but no significant change was observed in both powder and oil free seed powder   treated groups. On the 28th day seed powder treated group significantly lowered serum TG level (p<0.033) and 48% was lowered by oil free seed powder. It is concluded that seed powder as well as oil free seed powder lowered blood glucose level in Type 2 diabetic model rats. It seems to act as an insulinomimetic and/or insulin sensitizing agent. DOI: http://dx.doi.org/10.3329/dujps.v11i1.12482 Dhaka Univ. J. Pharm. Sci. 11(1): 19-24, 2012 (June)

Metformin protects against diabetes–induced heart injury and dunning prostate cancer model

2020 ◽  
pp. 096032712094745
Author(s):  
BB Bayrak ◽  
P Koroglu ◽  
O Karabulut Bulan ◽  
R Yanardag
Keyword(s):  
Prostate Cancer ◽  
Tissue Damage ◽  
Total Antioxidant Status ◽  
Experimental Period ◽  
Heart Tissue ◽  
Diabetic Rats ◽  
Control Group ◽  
Cancer Group ◽  
Total Antioxidant

In this study, both diabetes and Dunning prostate cancer were induced for the first time in Copenhagen rats in vivo. Thus, the effects of metformin against heart tissue damage of these rats were investigated by biochemical methods. Dunning prostate cancer was induced in Copenhagen rats using high metastatic MAT-LyLu cells. The rats were divided as follows: Control group: only injected with 0.9% NaCl for 14 days; Diabetic group: only injected single dose of streptozotocin (STZ) (65 mg/kg); Cancer group: subcutaneously (s.c) inoculated with 2 x 104 MAT-LyLu cells only; Diabetic + cancer (DC) group: inoculated with 2 x 104 MAT-LyLu cells and STZ injection, Cancer + metformin (CM) group: injected with metformin for 14 days after Mat-LyLu cells application; Diabetic + cancer + metformin (DCM) group: metformin administered for 14 days together with STZ and Mat-LyLu cells. At the end of the experimental period, heart tissues were taken. Reduced glutathione and total antioxidant status levels in heart tissues were decreased, whereas lipid peroxidation, advanced oxidized protein products, nitric oxide, homocysteine, and reactive oxygen species levels, total oxidant status and catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and xanthine oxidase activities increased in the diabetic, cancer and DC groups. Treatment with metformin reversed these effects. In conclusion, the present study shows that metformin has a protective effect against heart tissue damage in STZ-induced diabetic rats with Dunning prostate cancer.

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