scholarly journals An Improved Micropropagation Protocol byEx VitroRooting ofPassiflora edulisSims. f.flavicarpaDeg. through Nodal Segment Culture

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari ◽  
C. P. Ravindran
Keyword(s):  
Survival Rate ◽  
Clonal Propagation ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Nodal Segment ◽  
Ms Medium ◽  
Ex Vitro ◽  
Passiflora Edulis ◽  
Half Strength

A procedure for rapid clonal propagation ofPassiflora edulisSims. f.flavicarpaDeg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL−16-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21±1.13) primordial from the nodal meristems. Subculturing of these multiple shoots on the MS medium augmented with 1.0 mgL−1of each BAP and Kinetin (Kin) was successful for the multiplication of the shootsin vitrowith maximum numbers of shoots (25.73±0.06) within four weeks of incubation. Shoots were rooted best (7.13±0.56roots/shoots) on half strength MS medium supplemented with 2.0 mgL−1indole-3 butyric acid (IBA). Allin vitroregenerated shoots were rooted byex vitromethod, and this has achieved 6-7 roots per shoot by pulsing of cut ends of the shoots using 200 as well as 300 mgL−1IBA. The plantlets were hardened in the greenhouse for 4-5 weeks. The hardened plantlets were shifted to manure containing nursery polybags after five weeks and then transferred to a sand bed for another four weeks for acclimatization before field planting with 88% survival rate.

scholarly journals CLONAL PROPAGATION OF Gypsophila aretioides, AN IDEAL ROCK GARDEN PLANT SPECIES

2019 ◽  
Vol 18 (1) ◽  
pp. 39-45
Author(s):  
Leila Samiei ◽  
Mahboubeh Davoudi Pahnekolayi ◽  
Zahra Karimian
Keyword(s):  
Survival Rate ◽  
Clonal Propagation ◽  
Ground Cover ◽  
Root Induction ◽  
Ms Medium ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Garden Plant ◽  
Ornamental Species

Gypsophila aretioides, a cushion form evergreen plant, is a high potential wild species ideal for the use in rock garden, or as a ground cover in sunny dry areas. This plant has the competence to be developed as a new ornamental species. The purpose of this experiment was to provide an efficient micropropagation protocol for G. aretioides in order to facilitate the availability of this species for further studies of domestication. The influence of various concentrations of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) was investigated for multiplication stage. TDZ at low concentration of 0.05 mg dm−3 resulted in the maximum shoot (9.7) and leaf (42.3) number. The shoots were best rooted on MS medium containing 0.6 mg dm–3 indolebutyric acid (IBA) with 7.8 roots per shoot. Despite achievement of a successful protocol for in vitro multiplication and root induction of Gypsophila, low survival rate was obtained when rooted explants were exposed to ex vitro conditions. This is an important issue, which requires particular consideration and further studies. The possible reasons contributing to the low acclimatization rate of this species are being discussed.

scholarly journals In vitro Rapid Clonal Propagation of Rauvolfia serpentina (Linn.) Benth

1970 ◽  
Vol 42 (1) ◽  
pp. 37-44 ◽  
Author(s):  
R Baksha ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun ◽  
John Liton Munshi
Keyword(s):  
Large Scale ◽  
Clonal Propagation ◽  
Multiple Shoots ◽  
Direct Regeneration ◽  
Ms Medium ◽  
Rauvolfia Serpentina ◽  
Half Strength ◽  
In Vitro Clonal Propagation ◽  
Shoot Tip Explants

A protocol was developed for in vitro clonal propagation of Rauvolfia serpentina through direct regeneration from shoot tip explants. Multiple shoots (eight shoots per explant) induction were obtained on MS medium supplemented with 4.0 mg/l BAP and 0.5 mg/l NAA within 10-15 days. The elongated shoots rooted well in half strength MS medium with 0.5 mg/l NAA. The in vitro raised plantlets were acclimatized in glass house and successfully transplanted to field condition with 80 % survival. The results indicated that large scale commercial micropropagation of Rauvolfia serpentina is technically feasible. Bangladesh J. Sci. Ind. Res. 42(1), 37-44, 2007

scholarly journals Clonal propagation of Carob (Ceratonia siliqua L., Fabaceae)

1970 ◽  
Vol 39 (1) ◽  
pp. 15-19 ◽  
Author(s):  
L Hakim ◽  
MR Islam ◽  
ANK Mamun ◽  
G Ahmed ◽  
R Khan
Keyword(s):  
Clonal Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Axillary Buds ◽  
Root Induction ◽  
Ms Medium ◽  
Ceratonia Siliqua ◽  
Moderate Amount ◽  
Half Strength

Mature seeds of carob tree (Ceratonia siliqua L.) were germinated on hormone free MS medium. Efforts were made to develop multiple shoots by using axillary buds of in vitro grown seedlings on MS medium fortified with different concentrations of BA singly and BA in combination with IAA or GA3. Axillary buds produced single shoot with a moderate amount of callus at the base of the explant after culturing on MS medium with BA alone. Multiple shoots were regenerated when explants when cultured on MS medium fortified with BA + IAA or BA + GA3. MS medium supplemented with 1.5 mg/l BA + 0.5 mg/l GA3 was found more effective in multiple shoot regeneration than all other combinations. Regenerated multiple shoots were excised and cultured on half strength of MS medium containing different concentrations of IBA for root induction. Best root development was obtained in half strength MS medium containing 0.5 mg/l IBA. About 70% of the regenerated plantlets survived in natural conditions. Key words: Carob; Ceratonia siliqua; Fabaceae; Clonal propagation DOI: 10.3329/bjb.v39i1.5520Bangladesh J. Bot. 39(1): 15-19, 2010 (June)

scholarly journals In vitro multiplication, micromorphological studies and ex vitro rooting of Hybanthus enneaspermus (L.) F. Muell. – a rare medicinal plant

2018 ◽  
Vol 77 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Nodal Segments ◽  
Ms Medium ◽  
Ex Vitro ◽  
Culture Bottle ◽  
Hybanthus Enneaspermus ◽  
Half Strength

AbstractHybanthus enneaspermusis a rare medicinal plant. We defined a protocol for micropropagation,ex vitrorooting of cloned shoots and their acclimatization. Surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin). Medium supplemented with 1.5 mg L−1BAP was found optimum for shoot induction from the explants and 6.4±0.69 shoots were regenerated from each node with 97% response. Shoots were further proliferated maximally (228±10.3 shoots per culture bottle with 7.5±0.43 cm length) on MS medium augmented with 1.0 mg L−1each of BAP and Kin within 4–5 weeks. The shoots were rootedin vitroon half strength MS medium containing 2.0 mg L−1indole-3 butyric acid (IBA). The cloned shoots were pulse-treated with 300 mg L–1 of IBA and cultured on soilrite® in a greenhouse. About 96% of the IBA-pulsed shoots rootedex vitroin soilrite®, each shoot producing 12.5±0.54 roots with 5.1±0.62 cm length. Theex vitrorooted plantlets showed a better rate of survival (92%) in a field study thanin vitrorooted plantlets (86%). A comparative foliar micromorphological study ofH. enneaspermuswas conducted to understand the micromorphological changes during plant developmental processes fromin vitrotoin vivoconditions in terms of variations in stomata, vein structures and spacing, and trichomes. This is the first report onex vitrorooting inH. enneaspermusand the protocol can be exploited for conservation and large-scale propagation of this rare and medicinally important plant.

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

2015 ◽  
pp. 99-112
Author(s):  
Marija Markovic ◽  
Dragana Skocajic ◽  
Mihailo Grbic ◽  
Matilda Djukic ◽  
Dragica Obratov-Petkovic ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Nutrient Solution ◽  
Ph Value ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Efficient Production ◽  
Ms Medium ◽  
Ex Vitro ◽  
Half Strength

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

scholarly journals Plant Regeneration Through Nodal Explant Derived Callus in Wood Apple (Aegle marmelos L.)

1970 ◽  
Vol 44 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Ranjoy Das ◽  
M Faruk Hasan ◽  
Harunar Rashid ◽  
Motiur Rahman
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Nodal Explant ◽  
Aegle Marmelos ◽  
Half Strength ◽  
Subsequent Regeneration

This study reports on an improved protocol for callus induction and subsequent regeneration from nodal segment of wood apple (Aegle marmelos L.) Creamish friable competent callus was achieved from nodal segments on MS medium augmented with 4.0 mg1-1 2,4-D within two weeks of inoculation. The callus produced large number of shoots when cultured on MS medium fortified with 2.0 mgl-1 BAP+0.1 mgl-1 NAA within ten days of culture. In vitro raised shoots were rooted on half strength MS medium enriched with 1.0 mgl-1 IBA within fifteen days of culture. The rooted plantlets were successfully established with 80% survival. Key words: Plant regeneration; Callus induction; Nodal explant; Aegle marmelos. DOI: 10.3329/bjsir.v44i4.4590 Bangladesh J. Sci. Ind. Res. 44(4), 415-420, 2009

scholarly journals In Vitro Regeneration of Phyllanthus amarus Schum. and Thonn.: An Important Medicinal Plant

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 110-115 ◽  
Author(s):  
A. Sen ◽  
M.M. Sharma ◽  
D. Grover ◽  
A. Batra
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Phyllanthus Amarus ◽  
Regenerated Plants ◽  
Half Strength ◽  
Important Medicinal Plant

An efficient in vitro plant regeneration protocol was developed for the medicinally potent plant species Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) using nodal segment as explant. Maximum multiplication of shoots (15.275±0.96) was achieved on Murashige and Skoog’s medium supplemented with BAP (0.5 mg/l) after 3-4 weeks of inoculation. The shoots were separated from cluster and subcultured for their elongation on the same medium. In vitro flowering was also observed on the elongated shoots after 3–4 weeks of sub culturing on the shoot elongation medium. In vitro rooting was obtained on half strength MS medium supplemented with IBA (0.5 mg/l).  Regenerated plants were successfully hardened and acclimatized, 80 % of plantlets survived well under natural conditions after transplantation.Key words: In vitro regeneration, multiple shoots, nodal segments, Phyllanthus amarusDOI: 10.3126/on.v7i1.2557Our Nature (2009) 7:110-115

scholarly journals An efficient in vitro regeneration protocol to generate stable transgenic lines of Black gram (Vigna mungo)

2018 ◽  
Vol 78 (3) ◽  
Author(s):  
Bidyut Kumar Sarmah ◽  
Trishna Konwar ◽  
Borsha Borah ◽  
Arun Kumar Handique ◽  
Sumita Acharjee
Keyword(s):  
Transformation Efficiency ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Vigna Mungo ◽  
Ms Medium ◽  
Black Gram ◽  
Transgenic Lines ◽  
Half Strength ◽  
The Individual

An efficient and quick in vitro regeneration protocol was developed for black gram (Vigna mungo) using wounded embryonic axis with cotyledon as explant. Murashige and Skoog (MS) medium supplemented with 4.44 μM BAP and 2.32 μM Kinetin was found to be effective in producing maximum number (mean 7.80) of multiple shoots. The individual shoots elongated to 4.5 cm when MS medium was supplemented with 2.89 μM GA3 along with 0.44 μM BAP and 0.46 μM KIN. A novel in vitro rooting technique was also optimized for black gram using half-strength liquid MS medium supplemented with 1.34 μM NAA. The shoots in this medium produced the highest number (mean 7.50) of roots with root length of 6.02 cm. The plantlets were transferred to soil mixture and placed in greenhouse where more than 80% successfully grew to maturity. The same protocol was successfully used to generate transgenic black gram lines carrying Bt-Cry2Aa gene through Agrobacteriummediated transformation with a transformation efficiency of 0.42%. The rooted T0 plants grew to maturity and produced T1 seeds with the presence and expression of transgene in T1 plants. Thus, we have standardized an in vitro regeneration protocol suitable for generation of stable transgenic black gram plants.

scholarly journals In vitro regeneration of two high-yielding eggplant (Solanum melongena L.) varieties of Bangladesh

1970 ◽  
pp. 08-12
Author(s):  
Sabina Yesmin, Mst Muslima Khatun, Tanzena Tanny ◽  
Anica Tasnim Protity ◽  
Md Salimullah ◽  
Iftekhar Alam
Keyword(s):  
In Vitro Regeneration ◽  
Solanum Melongena ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Healthy Plant ◽  
Ms Medium ◽  
Best Response ◽  
Half Strength ◽  
Multiple Shoot Regeneration

An in vitro regeneration protocol was developed for two high-yielding eggplant varieties (Solanum melongena L.) namely BARI begun-4 and BARI begun-6. Multiple shoots were regenerated from cotyledonary explants through organogenesis with growth regulators of different combinations and concentrations.  The best response towards multiple shoot regeneration was achieved from cotyledon explants on MS media complemented with 1 mg/l BAP + 0.2 mg/l IAA in both the two varieties of eggplant. Elongation of shoots was achieved on hormone free MS medium. Regenerated shoots of both the varieties produced   active in vitro root system on half strength of MS medium supplemented with 0.2 mg/l IBA.  The in vitro grown plantlets were acclimatized in soil, grew up to maturity, flowered, fruited and produced seeds as normal healthy plant like the control.

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