scholarly journals Using Fourier Transform Infrared Spectroscopy to Study Effects of Magnetic Field Treatment on Wheat (Triticum aestivumL.) Seedlings

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Zhenlin Wei ◽  
Dejie Jiao ◽  
Junxiao Xu
Keyword(s):  
Magnetic Field ◽  
Fourier Transform ◽  
Conformational Changes ◽  
Structural Variation ◽  
Fourier Transform Infrared ◽  
Biological Mechanisms ◽  
Wheat Seedlings ◽  
Wheat Leaves ◽  
Germination And Growth ◽  
Insight Into

Magnetic field treatments have been utilized to promote germination and growth of a variety of species of plants; however the mechanism of concern has not been fully elucidated. In this research, wheat seedlings were treated with 500 mT and 1500 mT static magnetic field (SMF) for 10 and 20 min, respectively. Analyzing Fourier transform infrared spectra collected from leaves of seedlings showed that SMF treatments decreased the contents of lipids and proteins, shifted bands to higher wavenumbers in 3000–2800 cm−1regions, and increased the ratio of CH2/CH3which likely indicates a structural variation of lipids. For bands assigned to different second structures of proteins, slight bands shifting and changing the ratio of different second structures of proteins were observed due to SMF treatments. To summarize, the results revealed that lipids rather than proteins were sensitive to SMF treatments. The results provided insight into the SMF induced conformational changes of lipids and proteins in wheat leaves, which will help elucidate the biological mechanisms of SMF on plant growth and development.

CORNEAL TOXICITY ASSOCIATED WITH AMMONIA EXPOSURE INVESTIGATED BY FOURIER TRANSFORM INFRARED SPECTROSCOPY

2009 ◽  
Vol 04 (04) ◽  
pp. 331-343 ◽  
Author(s):  
SHERIF SIDDICK MAHMOUD
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Conformational Changes ◽  
Biological Effects ◽  
Exothermic Reaction ◽  
Fourier Transform Infrared ◽  
Gaseous Ammonia ◽  
Tissue Water ◽  
Ammonia Exposure

Chemical injury to the eye is still an important cause of blindness and serious complications. Gaseous ammonia combines with tissue water to form ammonium hydroxide ( NH 4 OH ). This exothermic reaction results in both heat and chemical burns. Although, over the years, the different biological effects of anhydrous ammonia are well known, its ocular effects are less clearly documented. This study reports the corneal structural alterations that may be induced as a result of ammonia exposure (gas or liquid) that was studied by Fourier transform infrared spectroscopy. The resulted IR spectra were analyzed using the band enhancement procedure. The obtained data clearly indicate that there are different structural and conformational changes (includes lipids and proteins) as the method of exposure to the ammonia differ, and due to the increased ammonia occupational exposure, there is an insistent need for the development of ophthalmic medications.

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