scholarly journals Statistical Optimization of Conditions for Decolorization of Synthetic Dyes byCordyceps militarisMTCC 3936 Using RSM

2015 ◽  
Vol 2015 ◽  
pp. 1-17 ◽  
Author(s):  
Baljinder Kaur ◽  
Balvir Kumar ◽  
Neena Garg ◽  
Navneet Kaur
Keyword(s):  
Incubation Time ◽  
Liquid Culture ◽  
Ph Value ◽  
Preliminary Investigation ◽  
White Rot ◽  
White Rot Fungus ◽  
Synthetic Dyes ◽  
Culture Systems ◽  
Reactive Green 19 ◽  
Optimized Conditions

In the present study, the biobleaching potential of white rot fungusCordyceps militarisMTCC3936 was investigated. For preliminary screening, decolorization properties ofC. militariswere comparatively studied using whole cells in agar-based and liquid culture systems. Preliminary investigation in liquid culture systems revealed 100% decolorization achieved within 3 days of incubation for reactive yellow 18, 6 days for reactive red 31, 7 days for reactive black 8, and 11 days for reactive green 19 and reactive red 74. RSM was further used to study the effect of three independent variables such as pH, incubation time, and concentration of dye on decolorization properties of cell free supernatant ofC. militaris. RSM based statistical analysis revealed that dye decolorization by cell free supernatants ofC. militarisis more efficient than whole cell based system. The optimized conditions for decolorization of synthetic dyes were identified as dye concentration of 300 ppm, incubation time of 48 h, and optimal pH value as 5.5, except for reactive red 31 (for which the model was nonsignificant). The maximum dye decolorizations achieved under optimized conditions for reactive yellow 18, reactive green 19, reactive red 74, and reactive black 8 were 73.07, 65.36, 55.37, and 68.59%, respectively.

PCB’s Biotransformation by a White-Rot Fungus Under Composting and Liquid Culture Conditions

2002 ◽  
pp. 287-297
Author(s):  
G. Ruiz-Aguilar ◽  
J. Fernández-Sánchez ◽  
R. Rodríguez-Vázquez ◽  
H. M. Poggi-Varaldo ◽  
F. Esparza-García ◽  
...  
Keyword(s):  
Liquid Culture ◽  
Culture Conditions ◽  
White Rot ◽  
White Rot Fungus

Biodegradation of Bisphenol A and Decolorization of Synthetic Dyes by Laccase from White-Rot Fungus, Trametes polyzona

2012 ◽  
Vol 169 (2) ◽  
pp. 539-545 ◽  
Author(s):  
Thanunchanok Chairin ◽  
Thitinard Nitheranont ◽  
Akira Watanabe ◽  
Yasuhiko Asada ◽  
Chartchai Khanongnuch ◽  
...  
Keyword(s):  
Bisphenol A ◽  
White Rot ◽  
White Rot Fungus ◽  
Synthetic Dyes

scholarly journals Characteristics of Compost from Balinese Cattle Dung (CD) and Rice Straw (RS) using White Rot Fungus (Wrf) (Ganoderma sp) As Bioactivators

2020 ◽  
Vol 15 (3) ◽  
pp. 194-204
Author(s):  
Muhammad Irfan Said ◽  
Jamila Mustabi ◽  
Siti Amelia Putri Syamsuddin
Keyword(s):  
Rice Straw ◽  
Ph Value ◽  
White Rot ◽  
White Rot Fungus ◽  
Cattle Dung ◽  
Randomized Design ◽  
Test Parameters ◽  
The Difference ◽  
The Republic ◽  
Range Of Values

To maximize the performance of the fermentation process, there is a need for a biactivator. The purpose of this study was to evaluate the characteristics of compost-based waste from cattle dung (CD) combined with rice straw (RS) by applying white rot fungus (Wrf) (Ganoderma sp) as a bioactivator. In this study, 6 types of treatments have been applied, namely T1(CD:RS)(25%:75%)+5% of Wrf; T2(CD:RS)(50%:50%)+5% of Wrf; T3(CD:RS)(75%:25%)+5% of Wrf; T4 (CD:RS)(25%:25%)+0% of Wrf; T5(CD:RS)(50%:50%)+0% of Wrf; T6 (CD:RS)(75%:25%)+0% of Wrf. The study was designed experimentally using a completely randomized design (CRD), 6 treatments with 3 replications. Based on the research results, the difference in the ratio of CD to RS and the use of Wrf bioactivator has a significant effect (p<0.05) in increasing the pH, temperature, C-organic and C/N ratio in compost. However, this difference had no significant effect (p>0.05) on the C-organic value. The pH value of compost was obtained with variations of 5.50±0.43-6.73±0.06. Temperature of compost is in the range of values 24.00±0.00-25.67 ±0.58 ºC. The C-organic value is in the range of 24.38±0.48-31.32±0.80%, while the N-organic value varies at 1.34±0.11-2.10±0.15%. The C/N ratio is in the range of 15.00± 0.11-19.33±1.15. Based on the interaction, the results showed that there was a significant interaction (p>0.05) between the use of Wrf bioactivator with the ratio of CD to RS on the parameters of pH, N-organic and C/ Nratio, while, C-organic and temperature had no significant effect. The results of the study was concluded that several test parameters produced compost characteristics that were in accordance with Standar Nasional Indonesia (SNI) and the Regulation of the Minister of Agriculture of the Republic of Indonesia. Compost production using T3 treatment ((CD:RS)(75%:25%) + 5% of Wrf) produces the best characteristics compared to other treatments.

Comparison of Remazol Brillant Blue Removal from Wastewater by Two Different Organisms and Analysis of Metabolites by GC/MS

2014 ◽  
Vol 97 (5) ◽  
pp. 1416-1420 ◽  
Author(s):  
Hatice Ardag Akdogan ◽  
Merve Canpolat
Keyword(s):  
Biological Treatment ◽  
Extracellular Enzymes ◽  
Low Cost ◽  
Environmental Pollutants ◽  
White Rot ◽  
White Rot Fungus ◽  
Synthetic Dyes ◽  
Biological Degradation ◽  
Peroxidase Enzyme ◽  
Textile Industries

Abstract White rot fungus participates in biological degradation of many organic environmental pollutants. Also, white rot fungus contains a variety of extracellular enzymes, and these enzymes are used for biological degradation of organic matter. We investigated the biological treatment of synthetic dyes, at a low cost and in the shortest possible time, that are used especially in the dye and textile industries and are important polluting agents in the wastewater discharged into the environment by these industries. For this purpose, removal of Remazol Brillant Blue by Pleurotus ostreatus and Coprinus plicatilis was studied. This dye was removed 100% (dye concentration, 10.0 mg/L) by both organisms. Laccase and manganese peroxidase enzyme activities were also monitored. There was an attempt to identify metabolites via GC/MS at the end of the decolorization. No detectable metabolite was found.

Adsorption and degradation of synthetic dyes on the mycelium of Trametes versicolor

1998 ◽  
Vol 38 (4-5) ◽  
pp. 233-238 ◽  
Author(s):  
Yuxin Wang ◽  
Jian Yu
Keyword(s):  
Adsorption Capacity ◽  
Trametes Versicolor ◽  
White Rot ◽  
White Rot Fungus ◽  
Fungal Mycelium ◽  
Maximum Adsorption Capacity ◽  
Synthetic Dyes ◽  
Dye Molecules ◽  
Adsorption Affinity ◽  
Adsorbed Dye Molecules

Adsorption and degradation of three synthetic dyes with representative chromophores (azo, anthraquinone and indigo) were investigated on living mycelium of white rot fungus, Trametes versicolor. The maximum adsorption capacity (Qmax) and adsorption affinity (K) of the dead and living fungal mycelia to the three dyes were measured and estimated by using the Langmuir model; Qmax has a range from 50 to 105 mg dye/g dry mycelium and K from 17 to 120 mg dye/L. The adsorbed dye molecules could be degraded by the extracellular and/or intracellular enzymes that were produced by a 10-day old fungal mycelium after the essential nitrogen nutrient (NH4+) had been consumed completely. Fungal mycelium was saturated by the dyes in one hour and its adsorption capacity was regenerated at different rates depending on dye structure and enzymes. Compared to the enzymatic regeneration of dye-saturated living mycelium (8-19 mg dye/g dry mycelium. h), physical desorption of adsorbed dye molecules was consistent ranging from 2-3 mg dye/g dry mycelium. h.

Effects of White-Rot Fungus Trametes Sp.lg-9 Treatment on Properties of Chlorine-Containing Bleaching Wastewater

2012 ◽  
Vol 550-553 ◽  
pp. 2241-2245
Author(s):  
Zhi Qiang Pang ◽  
Chao Yang ◽  
Jia Chuan Chen ◽  
Gui Hua Yang
Keyword(s):  
Ph Value ◽  
Lignin Content ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Crude Enzyme ◽  
White Rot ◽  
Enzyme Treatment ◽  
White Rot Fungus ◽  
Short Time ◽  
Low Dosage

Effects of white-rot fungus Trametes sp.lg-9 treatment on properties of chlorine-containing bleaching wastewater were investigated. Trametes sp.lg-9 was cultured in chlorine-containing bleaching wastewater under optimal growth conditions, COD sharply decreased from 10740mg/l to 7811mg/l after 5 days; chroma firstly increased from 6212 degree to 7272 degree at 5 days and then declined to 5303 degree; lignin content firstly increased to 0.33 g/l and then decreased to 0.323 g/l. Separating the crude enzyme solution to treat chlorine-containing bleaching wastewater, demonstrating the suitable temperature of the crude enzyme treatment is 40 °C and the optimum pH value is 5.6. COD, chroma and lignin content of chlorine-containing bleaching wastewater can be significantly reduced by crude enzyme treatment on conditions of low dosage and short time.

scholarly journals Improvement of laccase activity by silencing PacC in Ganoderma lucidum

2021 ◽  
Author(s):  
Mingwen Zhao ◽  
Jing Zhu ◽  
Shuqi Song ◽  
Lindan Lian ◽  
Liang Shi ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Laccase Activity ◽  
Ph Value ◽  
Fold Increase ◽  
White Rot ◽  
White Rot Fungus ◽  
Copper Binding ◽  
Multicopper Oxidases ◽  
Binding Domains ◽  
Laccase Genes

Abstract Ganoderma lucidum is a representative white-rot fungus that has great potential to degrade lignocellulose biomass. Laccase is recognized as a class of the most important lignin-degrading enzymes in G. lucidum. However, the comprehensive regulatory mechanisms of laccase are still lacking. Based on the genome sequence of G. lucidum, 15 laccase genes were identified and their encoding proteins were analyzed in this study. All of the laccase proteins are predicted to be multicopper oxidases with conserved copper-binding domains. Most laccase proteins were secreted enzymes in addition to Lac14 in which the signal peptide could not be predicted. The activity of all laccases showed the highest level at pH 3.0 or pH 7.0, with total laccase activity of approximately 200 U/mg protein. Silencing PacC resulted in a 5.2 fold increase in laccase activity compared with WT. Five laccase genes (lac1, lac6, lac9, lac10 and lac14) showed an increased transcription levels (approximately 1.5-5.6 fold) in the PacC-silenced strains versus that in WT, while other laccase genes were downregulated or unchanged. The extracellular pH value was about 3.1, which was more acidic in the PacC-silenced strains than in the WT (pH 3.5). Moreover, maintaining the fermentation pH resulted in a downregulation of laccase activity which is induced by silencing PacC Our findings indicate that in addition to its function in acidification of environmental pH, PacC plays an important role in regulating laccase activity in fungi.

scholarly journals Biostoning of textile effluent with laccase enzyme

2021 ◽  
Vol 56 (2) ◽  
pp. 115-124
Author(s):  
K Iqbal ◽  
A Nadeem ◽  
U Zafar
Keyword(s):  
Synthetic Medium ◽  
Dye Decolorization ◽  
White Rot ◽  
Textile Effluent ◽  
White Rot Fungus ◽  
Synthetic Dyes ◽  
Aquatic Life ◽  
Predicted Values ◽  
Laccase Enzyme ◽  
Experimental Values

The incessant release of textile effluent comprehending dyes and heavy metals which impacts on aquatic life. Current study used an enzyme to eradicate phenolic compounds and synthetic dyes from textile effluent by an indigenously isolated white rot fungus Pleurotus ostreatus- P1 has been cultured and indicated the utmost laccase activity with synthetic medium; as reacting substrate for dye decolorization. The textile effluent decolorization optimization has been conducted with different concentrations of laccase enzyme, temperature and pH. The 90% effluent decolorization was obtained by applying response surface methodology (RSM) conditions temperature, crude laccase enzyme and pH, 25˚C, 13.5 U/ml and 5 respectively. The predicted values were validated with experimental values, that confirms the steadiness of the model. Bangladesh J. Sci. Ind. Res.56(2), 115-124, 2021

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