scholarly journals In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property ofScrophularia kakudensisFranch Tissue Extracts

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Abinaya Manivannan ◽  
Prabhakaran Soundararajan ◽  
Yoo Gyeong Park ◽  
Byoung Ryong Jeong
Keyword(s):  
Free Radical ◽  
In Vitro Propagation ◽  
Adventitious Shoots ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Survival Ratio ◽  
Ms Medium ◽  
Root Extracts

The current study deals with in vitro propagation, antioxidant property estimation, and assessment of acacetin content inScrophularia kakudensisFranch. Adventitious shoot induction was achieved from the nodal explant with the highest number of adventitious shoots per explant (17.4) on Murashige and Skoog’s (MS) medium fortified with 2.0 mg·L−16-benzyladenine (BA) and 0.5 mg L−1indole-3-acetic acid (IAA). Maximum number of roots per plant (16.5) was noted in half strength MS medium supplemented with 0.5 mg·L−1IAA. The regenerated plants displayed successful survival ratio (95%) in the greenhouse. The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g−1FW; in vivo: 30.05 µg·g−1FW) followed by root extracts. Total phenol and flavonoid contents along with free radical scavenging assays revealed the occurrence of larger amount of antioxidants in shoot extract in comparison with callus and root extracts ofS. kakudensis. Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation ofS. kakudensisfor therapeutic purposes.

scholarly journals PRELIMINARY PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF ARAUCARIA COLUMNARIS BARK PEEL AND COSMOS SULPHUREUS FLOWERS

2017 ◽  
Vol 9 (4) ◽  
pp. 96 ◽  
Author(s):  
Krishma M. Jadav ◽  
K. N. Ninge Gowda
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Phytochemical Composition ◽  
Dpph Method ◽  
In Vitro Antioxidant Activity

Objective: Four different extracts of Araucaria columnaris (bark peel) and Cosmos sulphureus (flowers) were screened for their phytochemical composition, and free radical scavenging activities.Methods: DPPH method was used to test the antioxidant activity for extracts.Results: Among the different extracts tested, the methanol extract of both the plant species showed significant radical scavenging activities. Phytochemical analysis of the extracts revealed that the radical scavenging activities might be due to the presence of flavonoids, tannins and phenolic compounds.Conclusion: The results obtained suggest that Araucaria columnaris (bark peel) and Cosmos sulphureus(flowers) could be exploited in the treatment of various diseases like cancer, cardiovascular diseases and infection diseases.  

scholarly journals In vitro propagated Mentha rotundifolia (L.) Huds and antioxidant activity of its essential oil

2018 ◽  
Vol 31 (4) ◽  
pp. 204-208 ◽  
Author(s):  
Amira Benahmed ◽  
Boualem Harfi ◽  
Ibtissem Benbelkacem ◽  
Abla Daas ◽  
Hocine Laouer ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Dry Matter ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antioxidant Potential ◽  
Wild Plant ◽  
Stem Length ◽  
Ms Medium

Abstract Mentha rotundifolia (L.) Huds is an aromatic plant used for its medicinal values. This study aims to select appropriate conditions for in vitro propagation of M. rotundifolia (L.) Huds and to evaluate yield and antioxidants activity of its essential oils (EOs). The explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators 6-benzylaminopurine (BAP) and Gibberellin (GA3). Hydrodistillated EOs obtained from acclimatized and mother plant, were evaluated for their antioxidant activity. Tests were performed on DPPH free radical-scavenging, ABTS and CUPRAC assays. Shoot induction and multiplication were successfully carried out on MS medium supplemented with the following hormones combinations: 1 mg/l BAP, 1 mg/l GA3 and 0.5 mg/l BAP, 0.5 mg/l GA3, respectively. Stem length, nodes and leaves number measured from development vitroplant were 6.89 cm, 5.22 nodes and 11.92 leaves per vitroplant, respectively. In vitro rooted plants were successfully acclimatized at a temperature of 23 ± 2°C and a long day photoperiod with a total survival rate exceeding 95%. EO yield of acclimated plant varied between (0.88-1.49 ml/100 g dry matter) compared to wild plant (0.73 ml/100 g dry matter). The antioxidant potential of EOs from acclimated plant showed on DPPH free radical-scavenging, ABTS and CUPRAC assays values of (IC50: 4.18-24.93 mg/ml), (IC50: 0.51-1.56 mg/ml) and (A0.50: 0.34-2.71 mg/ml), respectively. In contrast, the wild plant exhibited on the same tests the values of (IC50: 10.35 mg/ml), (IC50: 0.12 mg/ml) and (A0.50: 0.99 mg/ml), respectively. The results suggest that micropropagation of M. rotundifolia (L.) Huds can be an interesting alternative for producing important plant material with the possibility to modulate EO yield and its antioxidant potential for future commercial purposes.

Evaluation of In-vitro Antioxidant Potential and Screening, Characterization of Phytochemicals Using hydroethanolic leaf Extract of Caesalpinia Pulcherrima

2020 ◽  
Vol 09 ◽  
Author(s):  
Akila Ilangovan ◽  
Raghu V ◽  
Venugopal R ◽  
Vijay N ◽  
Yellasamy Y ◽  
...  
Keyword(s):  
Free Radical ◽  
Functional Groups ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Phytochemical Analysis ◽  
Scavenging Activity

Introduction: Novel Therapeutic Phytochemical studies are based on the use of plants in the production of drugs. This present study was carried out to the screening of phytochemicals and quantitative analysis, to assess In-vitro Antioxidant activities of the hydroethanolic leaf extract of Caesalpinia pulcherrima. FT-IR and GC-MS analysis of hydroethanolic leaf extract of Caesalpinia pulcherrima. Methods: The investigation of qualitative and determination of quantitative phytochemical analysis were performed using standard procedures. The total alkaloid, flavonoid, phenols, and tannin content were determined spectrophotometrically. DPPH (2,2-Diphenyl-1-picrylhydrazyl) free radical scavenging activity, ABTS (2,2’-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) free radical scavenging activity of hydroethanolic leaf extract of Caesalpinia pulcherrima was estimated by standard protocol. The identification of functional groups using plant leaf extracts by FTIR and GCMS. Results: The hydroethanolic leaf extract of Caesalpinia pulcherrima gives positive results for alkaloids, flavonoids, phenols, carbohydrates, tannins, amino acids, and proteins. The content of total alkaloids, flavonoids, phenols, and tannins varies from 8.4 AE/g, 3.3 CE/g, 2.9, and 3.2 GAE/g respectively. The antioxidant assay and IC50 values were found to be DPPH (43.3µg/ml), ABTS (46.1µg/ml), and ascorbic acid (41µg/ml), respectively and concentration ranging from 20 to 100µgml. Functional groups were also identified using the extract by FTIR and GCMS. Conclusions: From this work, it can be concluded that the presence of Phyto-component makes the plant useful in the treatment of various diseases. As such, it has been found that the hydroethanolic leaf extract of the plant contains more components and is beneficial for further research.

scholarly journals In vitro oocysticidal sporulation inhibition of Eimeria tenella and antioxidant efficacy of ethanolic and aqueous extracts of Conyza aegyptiaca

2021 ◽  
Vol 6 (1) ◽  
pp. 30-40
Author(s):  
Emmanuel Tana Toah ◽  
◽  
Vincent Khan Payne1, ◽  
Yamssi Cedric ◽  
Noumedem Anangmo Christelle Nadia ◽  
...  
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Hot Water ◽  
Phytochemical Analysis ◽  
Aqueous Extracts

Avian coccidiosis is probably one of the most expensive parasitic diseases with major economic impact on poultry industries worldwide. The purpose of this study was to evaluate the ethanolic and aqueous extracts of Conyza aegyptiaca in terms of phytonutrients, in vitro oocysts sporulation inhibition and antioxidant properties. The extraction process of plant leaf powder (100 g) pulverized using a clean manual grinder was carried out in ethanol and hot water and the yields were calculated as a percentage ratio of extract mass on plant powder mass after solvent evaporation. Phytochemical analysis procedures were performed to determine the presence of phytonutrients. The in vitro oocysticidal sporulation inhibition was determined at five different concentrations (0.25; 0.5; 1; 2 and 4 mg/ml) of each extract in petri dishes each containing 3000 unsporulated oocysts and examined after 24 and 48 hours under a microscope. In vitro antioxidant capacity of extracts was estimated using different assays. Quantitative aqueous extract (11.72%) was higher than ethanolic extract (4.34%). In terms of qualitative yields, ethanolic extract revealed higher phytonutrients investigated (100%) than aqueous extract (42.86%). The sporulation inhibition of ethanolic extract was generally higher than the aqueous extract after 24 and 48 h and varied according to the different tested concentrations. In all the antioxidant assays, ethanolic extract exhibited significant free radical scavenging activity with inhibitory concentration (IC50=26.10±1.09) close to that of ascorbic acid at the probability level of 5% error (p<0.05). The ethanolic extract with higher free radical scavenging activities and ferric reducing effect also showed significant higher content of both phenols (127.01±3.99 mgGAE/g) and flavonoids (108.66±3.49 mgCE/g) than aqueous extracts, suggesting correlation between phenolic content and antioxidant activity. Data from this study could be used for developing bioactive elements for natural anticoccidials and antioxidants of health promoting activities

scholarly journals PHYTOCHEMICAL ANALYSIS AND IN VITRO FREE RADICAL SCAVENGING ACTIVITIES OF MEDICAGO SATIVA SEEDS

2015 ◽  
Vol 2 (1) ◽  
pp. 128-132
Author(s):  
Gomathi R ◽  
Banu S ◽  
Usha K
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Medicago Sativa ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Free Radical Scavenging Activities

Phytochemical analysis and in vitro free radical scavenging activities were analyzed in the various extracts of Medicago sativa seeds. The phytochemical analysis showed the presence of alkaloids, carbohydrates, flavonoids, glycosides, saponins, phytosterols, tannins, terpenoids and phenols. Among the various extracts, phytochemicals were extracted best in ethanol. Free radical scavenging activities such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, superoxide, 2,2'-azino-bis (3-ethylbenzothiazoline-6- sulphonic acid) (ABTS), ferrous ion chleating activity and non radicals such as hydrogen peroxide and nitric oxide were analyzed in the various extracts of Medicago sativa seeds and were compared with standard antioxidant ascorbic acid. All the extracts of Medicago sativa seeds scavenged the free radicals in a concentration dependent manner. The antioxidative activity of all the extracts was found to be more pronounced than that of the standard antioxidant ascorbic acid. Among the various extracts, the antioxidant activity was found to be more pronounced in ethanolic extract of Medicago sativa seeds

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