scholarly journals Antibacterial Activity of Synthetic Peptides Derived from Lactoferricin againstEscherichia coliATCC 25922 andEnterococcus faecalisATCC 29212

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
María A. León-Calvijo ◽  
Aura L. Leal-Castro ◽  
Giovanni A. Almanzar-Reina ◽  
Jaiver E. Rosas-Pérez ◽  
Javier E. García-Castañeda ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Synthetic Peptides ◽  
Unnatural Amino Acids ◽  
Low Cost ◽  
High Yield ◽  
E Coli ◽  
Rp Hplc ◽  
Bovine Lactoferricin ◽  
High Degree ◽  
Number Of Branches

Peptides derived from human and bovine lactoferricin were designed, synthesized, purified, and characterized using RP-HPLC and MALDI-TOF-MS. Specific changes in the sequences were designed as (i) the incorporation of unnatural amino acids in the sequence, the (ii) reduction or (iii) elongation of the peptide chain length, and (iv) synthesis of molecules with different number of branches containing the same sequence. For each peptide, the antibacterial activity againstEscherichia coliATCC 25922 andEnterococcus faecalisATCC 29212 was evaluated. Our results showed that Peptides I.2 (RWQWRWQWR) and I.4 ((RRWQWR)4K2Ahx2C2) exhibit bigger or similar activity againstE. coli(MIC 4–33 μM) andE. faecalis(MIC 10–33 μM) when they were compared with lactoferricin protein (LF) and some of its derivate peptides as II.1 (FKCRRWQWRMKKLGA) and IV.1 (FKCRRWQWRMKKLGAPSITCVRRAE). It should be pointed out that Peptides I.2 and I.4, containing the RWQWR motif, are short and easy to synthesize; our results demonstrate that it is possible to design and obtain synthetic peptides that exhibit enhanced antibacterial activity using a methodology that is fast and low-cost and that allows obtaining products with a high degree of purity and high yield.

scholarly journals Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Antimicrobial Activity against E. coli ATCC 11775, S. maltophilia ATCC 13636 and S. enteritidis ATCC 13076

Molecules ◽  
2017 ◽  
Vol 22 (3) ◽  
pp. 452 ◽  
Author(s):  
Nataly Huertas Méndez ◽  
Yerly Vargas Casanova ◽  
Anyelith Gómez Chimbi ◽  
Edith Hernández ◽  
Aura Leal Castro ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Synthetic Peptides ◽  
E Coli ◽  
Bovine Lactoferricin

scholarly journals ANTIBACTERIAL ACTIVITY FOR BIOSURFACTANT PRODUCED BY STREPTOMYCES SPP. ISOLATED FROM SOIL SAMPLES

2020 ◽  
pp. 109-110
Author(s):  
SAMER M. AL-HULU
Keyword(s):  
Antibacterial Activity ◽  
Aerial Mycelium ◽  
Inhibition Zone ◽  
Activity Analysis ◽  
Soil Samples ◽  
Blood Agar ◽  
Malt Extract ◽  
E Coli ◽  
Streptomyces Spp ◽  
High Degree

Objective: The goal of the present study on isolation Streptomyces from soil samples with biosurfactant activity and antibacterial activity analysis. Methods: (25) The soil samples were taken form Hilla city. (10) It is Streptomyces spp. They were identified. (4) Streptomyces spp. having the ability to hemolysis on blood agar that has the capacity to generate biosurfactants. Streptomyces spp. 8 displayed a high degree of activity by having a blood agar inhibition zone (25 mm). Results: Antibacterial activity was evaluated for Streptomyces spp. 8 (Staphylococcus aureus, Escherichia Coli, and Pseudomonas aeruginosa). Streptomyces spp. 8 has greatest inhibition zone against S. aureus equal to 14 mm compared 12 mm against E. coli and 8 mm for P. aeruginosa. This Streptomyces spp. 8 characteristic was tested, it showed Gram positive with aerial mycelium gray in color on yeast malt extract agar. Negative for melanin produced on tyrosine broth medium, negative for H2S output, and pigment development, it has for the use of mannitol sucrose, glucose, and fructose as carbon source and negative for xylose. Conclusion: Isolated Streptomyces spp. having the potential to generate biosurfactants with antibacterial activity.

scholarly journals Hydrothermal Fabrication of Spindle-Shaped ZnO/Palygorskite Nanocomposites Using Nonionic Surfactant for Enhancement of Antibacterial Activity

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1453 ◽  
Author(s):  
Aiping Hui ◽  
Shuqing Dong ◽  
Yuru Kang ◽  
Yanmin Zhou ◽  
Aiqin Wang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Low Cost ◽  
Ionic Surfactants ◽  
E Coli ◽  
Antibacterial Performance ◽  
Good Antibacterial Activity ◽  
Controllable Growth ◽  
And Staphylococcus Aureus

In order to improve the antibacterial performance of natural palygorskite, spindle-like ZnO/palygorskite (ZnO/PAL) nanocomposites with controllable growth of ZnO on the surface of PAL were prepared in the presence of non-ionic surfactants using an easy-to-operate hydrothermal method. The obtained ZnO/PAL nanocomposites have a novel and special spindle-shaped structure and good antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), and are also low cost. The minimum inhibitory concentrations of ZnO/PAL nanocomposites toward E. coli and S. aureus reached 1.5 and 5 mg/mL, respectively.

scholarly journals Structure-Function Relationship of Antibacterial Synthetic Peptides Homologous to a Helical Surface Region on Human Lactoferrin against Escherichia coli Serotype O111

1998 ◽  
Vol 66 (6) ◽  
pp. 2434-2440 ◽  
Author(s):  
Daniel S. Chapple ◽  
David J. Mason ◽  
Christopher L. Joannou ◽  
Edward W. Odell ◽  
Vanya Gant ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Transmission Electron Microscopy ◽  
Flow Cytometry ◽  
Antibacterial Activity ◽  
Synthetic Peptides ◽  
Membrane Integrity ◽  
E Coli ◽  
Transmission Electron ◽  
Helical Region

ABSTRACT Lactoferricin includes an 11-amino-acid amphipathic alpha-helical region which is exhibited on the outer surface of the amino-terminal lobe of lactoferrin. Synthetic peptides homologous to this region exhibited potent antibacterial activity against a selected range of both gram-negative and gram-positive bacteria. An analog synthesized with methionine substituted for proline at position 26, which is predicted to disrupt the helical region, abolished antibacterial activity against Escherichia coli and considerably reduced antibacterial activity against Staphylococcus aureus and anAcinetobacter strain. The mode of action of human lactoferrin peptide (HLP) 2 against E. coli serotype O111 (NCTC 8007) was established by using flow cytometry, surface plasmon resonance, and transmission electron microscopy. Flow cytometry was used to monitor membrane potential, membrane integrity, and metabolic processes by using the fluorescent probes bis-1,3-(dibutylbarbituric acid)-trimethine oxonol, propidium iodide, and carbonyl cyanide m-chlorophenylhydrazone, respectively. HLP 2 was found to act at the cell membrane, causing complete loss of membrane potential after 10 min and of membrane integrity within 30 min, with irreversible damage to the cell as shown by rapid loss of viability. The number of particles, measured by light scatter on the flow cytometer, dropped significantly, showing that bacterial lysis resulted. The peptide was shown to bind toE. coli O111 lipopolysaccharide by using surface plasmon resonance. Transmission electron microscopy revealed bacterial distortion, with the outer membrane becoming detached from the inner cytoplasmic membrane. We conclude that HLP 2 causes membrane disruption of the outer membrane, resulting in lysis, and that structural considerations are important for antibacterial activity.

scholarly journals Antibacterial Activity and Physical Properties of Fish Gelatin-Chitosan Edible Films Supplemented with D-Limonene

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Yunzhen Yao ◽  
Ding Ding ◽  
Hongyuan Shao ◽  
Qifan Peng ◽  
Yaqin Huang
Keyword(s):  
Antibacterial Activity ◽  
High Performance ◽  
Food Packaging ◽  
Low Cost ◽  
Edible Films ◽  
Water Soluble ◽  
Prepared Film ◽  
Fish Gelatin ◽  
Elongation At Break ◽  
E Coli

Fish gelatin-chitosan edible films with D-limonene were successfully prepared, which exhibited exceptional mechanical properties and antimicrobial activity. It has been demonstrated that water-soluble chitosan, fish gelatin, and D-limonene could be a candidate precursor to prepare low cost and high-performance edible food packaging material. The results showed that D-limonene in the films could effectively resist the penetration of light and water because of its hydrophobicity. Moreover, the elongation at break (EAB) increased with the addition of D-limonene, which indicated that D-limonene served as a strong plasticizer for the film. Microscopic characterization showed that D-limonene was uniformly distributed in the as-prepared film. And we found that the film exhibited strong antibacterial activity against Escherichia coli (E. coli). All the results indicate that the as-prepared film could be a promising food packaging.

scholarly journals Biochemical Properties of an Alkaline Xylanase From Bacillus Agaradhaerens C9 and Its Application in Producing Xylo-oligosaccharides With High Degree of Polymerization From Wheat Bran

2021 ◽  
Author(s):  
Jiawen Liu ◽  
Di Sun ◽  
Zhen Dong ◽  
Cong Liu ◽  
Jingrong Zhu ◽  
...  
Keyword(s):  
Wheat Bran ◽  
Low Cost ◽  
Maximal Activity ◽  
Biochemical Properties ◽  
Degree Of Polymerization ◽  
Single Step ◽  
Transition Elements ◽  
E Coli ◽  
High Degree ◽  
Bacillus Agaradhaerens

Abstract A xylanase of Bacillus agaradhaerens C9 was heterologously expressed and was then investigated. The recombinant xylanase (rBaxyl11) showed maximal activity at 60°C and pH 8.0-9.0. Under optimal conditions, Kcat of rBaxyl11 for arabinoxylan and glucuronoxylan were 599 s-1 and 330 s-1, respectively. rBaxyl11 showed a good stability at pH ranging from 5.0 to 9.0, and retained 50% of activity after 6-hour incubation at 70°C. However, it was markedly inactivated by transition elements including Fe3+, Ni2+, Mn2+, Co2+, Zn2+, Cu2+ and Fe2+. rBaxyl11 generated xylo-oligosaccharides (XOS) whose degree of polymerization (DP) is greater than 3 when hydrolyzing arabinoxylan, while the DP of XOS ranged from 2 to 6 when acting on glucuronoxylan. Simultaneously producing xylanase and XOS by recombinant E. coli containing rBaxyl11 were then carried out. Results showed that the engineering E. coli generated xylanase and high-DP XOS extracellularly using wheat bran as substrate, and concentration of XOS reached 73 mg/g substrate after 12-hour fermentation. This study indicates the feasibility of producing XOS by a single-step fermentation approach with low cost using rBaxyl11.

scholarly journals An Efficient Synthesis of Novel 3-[(Heteroaryl-2-ylimino)-methyl]-4-hydroxy-chromen-2-ones and Analogue of Tetrazole Derivatives and Their Antibacterial Activity

Molbank ◽  
10.3390/m1303 ◽  
2021 ◽  
Vol 2021 (4) ◽  
pp. M1303
Author(s):  
Ramiz Hoti ◽  
Hamit Ismaili ◽  
Veprim Thaçi ◽  
Gjyle Mulliqi-Osmani ◽  
Malësore Pllana-Zeqiri ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Condensation Reaction ◽  
High Yield ◽  
Cyclization Reactions ◽  
Spectrometric Data ◽  
E Coli ◽  
Polar Groups ◽  
The Impact ◽  
Tetrazole Derivatives

Synthesis of a series of the substituted [(pyridinyl and pyrimidin-2-ylimino)-ethyl]-4-hydroxy-chromen-2-ones and their tetrazole derivates is presented in this study. By catalytic condensation of 4-hydroxy-3-acetylcoumarine 2 and 2-aminopyridines 3(a-d), 3-[(pyridin-2-ylimino)-ethyl]-4-hydroxy-chromen-2-ones 4(a-d) are synthesized in high yield. During the condensation reaction of 2 and 4-amino-2,6-dihydroxypyrimidine 3e, 3-[1-(2,6-Dihydroxy-pyrimidin-4-ylimino)-ethyl]-4-hydroxy-chromen-2-one 4e as condensation products is synthesized. In following series, by cyclization reactions of compounds 4 (a-e) with sodium azide, analogue 3-substituted pyridin-2-yl and pyrimidin-2-yl-5-methyl-2,5-dihydro-1H-tetrazol-5-yl]-4-hydroxy-chromen-2-one 5(a-e) are synthesized the products. Structural characterization of the synthesized products is done on the basis of spectrometric data. Antibacterial activity of the compounds 4(a-e) and 5(a-e) against S. aureus, E. coli and Klebsiella was examined by measuring the inhibition zones around the disks marked with the corresponding products solution. The impact of substitutions in antimicrobial is also explored. Compounds with polar groups have shown significant antibacterial activity against these microorganisms.

Purification of Urokinase from Complex Mixtures Using Immobilized Monoclonal Antibody Against Urokinase Light Chain

1983 ◽  
Vol 49 (01) ◽  
pp. 024-027 ◽  
Author(s):  
David Vetterlein ◽  
Gary J Calton
Keyword(s):  
Monoclonal Antibody ◽  
Light Chain ◽  
Culture Media ◽  
Biological Fluids ◽  
Single Step ◽  
High Yield ◽  
Step Method ◽  
Commercial Preparation ◽  
E Coli ◽  
Tissue Culture Media

SummaryThe preparation of a monoclonal antibody (MAB) against high molecular weight (HMW) urokinase light chain (20,000 Mr) is described. This MAB was immobilized and the resulting immunosorbent was used to isolate urokinase starting with an impure commercial preparation, fresh urine, spent tissue culture media, or E. coli broth without preliminary dialysis or concentration steps. Monospecific antibodies appear to provide a rapid single step method of purifying urokinase, in high yield, from a variety of biological fluids.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

Bioenhancing Effect of Cow Urine Distillate and Pepper Extract on Antibacterial Activity of Azadirachta Indica Leaves

1970 ◽  
Vol 9 (2) ◽  
pp. 3212-3214
Author(s):  
Pramod Dhakal ◽  
Ankit a Achary ◽  
Vedamurthy Joshi
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Watery Diarrhea ◽  
Diffusion Method ◽  
E Coli ◽  
Drug Molecules ◽  
Cow Urine

Bioenhancers are drug facilitator which do not show the typical drug activity but in combination to enhance the activity of other molecule in several way including increase the bioavailability of drug across the membrane, potentiating the drug molecules by conformational interaction, acting as receptor for drug molecules and making target cell more receptive to drugs and promote and increase the bioactivity or bioavailability or the uptake of drugs in combination therapy. The objective of the present study was to evaluate the antibacterial and activity of combination in Azadirachta indica extract with cow urine distillate and pepper extract against common pathogenic bacteria, a causative agent of watery diarrhea. It has been found that Indian indigenous cow urine and its distillate also possess bioenhancing ability. Bioenhancing role of cow urine distillate (CUD) and pepper extract was investigated on antibacterial activity of ethanol extract of Azadirachta indica. Antibacterial activity of ethanol extract neem alone and in combination with CUD and pepper extract were determined the ATCC strains against Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and E-coli by cup plate diffusion method. Ethanol extract of neem has showed more effect on P. aeruginosa, E-coli than S. aureus and K. pneumonia with combination of CUD and pepper extract. CUD and pepper did not show any inhibition of test bacteria in low concentration. The antibacterial effect of combination of extract and CUD was higher than the inhibition caused by extract alone and is suggestive of the bioenhancing role of cow urine distillate and pepper. Moreover, inhibition of test bacteria was observed with less concentration of extract on combining with CUD

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