scholarly journals Anaplastic Thyroid Carcinoma: A ceRNA Analysis Pointed to a Crosstalk betweenSOX2,TP53, and microRNA Biogenesis

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Walter Arancio ◽  
Valeria Carina ◽  
Giuseppe Pizzolanti ◽  
Laura Tomasello ◽  
Maria Pitrone ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Thyroid Carcinoma ◽  
Cell Cycle Control ◽  
Control Cell ◽  
Anaplastic Thyroid Carcinoma ◽  
Chemotherapeutic Agents ◽  
Mitochondrial Activity ◽  
Microrna Biogenesis

It has been suggested that cancer stem cells (CSC) may play a central role in oncogenesis, especially in undifferentiated tumours. Anaplastic thyroid carcinoma (ATC) has characteristics suggestive of a tumour enriched in CSC. Previous studies suggested that the stem cell factorSOX2has a preeminent hierarchical role in determining the characteristics of stem cells in SW1736 ATC cell line. In detail, silencing SOX2 in SW1736 is able to suppress the expression of the stem markers analysed, strongly sensitizing the line to treatment with chemotherapeutic agents. Therefore, in order to further investigate the role of SOX2 in ATC, a competing endogenous RNA (ceRNA) analysis was conducted in order to isolate new functional partners of SOX2. Among the interactors, of particular interest are genes involved in the biogenesis of miRNAs (DICER1, RNASEN,andEIF2C2), in the control cell cycle (TP53, CCND1), and in mitochondrial activity (COX8A). The data suggest that stemness, microRNA biogenesis and functions, p53 regulatory network, cyclin D1, and cell cycle control, together with mitochondrial activity, might be coregulated.

Role of cell cycle control in radiosensitization of mouse spermatogonial stem cells

2001 ◽  
Vol 77 (3) ◽  
pp. 357-363 ◽  
Author(s):  
P. P. W. Van Buul ◽  
A. Van Duyn-Goedhart ◽  
T. Beumer ◽  
A. L. Bootsma
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Cell Cycle Control ◽  
Spermatogonial Stem Cells

The role of MCM proteins in the cell cycle control of genome duplication

BioEssays ◽  
1996 ◽  
Vol 18 (3) ◽  
pp. 183-190 ◽  
Author(s):  
Stephen E. Kearsey ◽  
Domenico Maiorano ◽  
Eddie C. Holmes ◽  
Ivan T. Todorov
Keyword(s):  
Cell Cycle ◽  
Genome Duplication ◽  
Cell Cycle Control ◽  
Mcm Proteins

scholarly journals METTL3-mediated M6a Modification Regulates Cell Cycle Progression of Dental Pulp Stem Cells

2021 ◽  
Author(s):  
Haiyun Luo ◽  
Wenjing Liu ◽  
Yanli Zhang ◽  
Xiao Jiang ◽  
Shiqing Wu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Flow Cytometry ◽  
Differentially Expressed Genes ◽  
Dental Pulp ◽  
Cell Cycle Control ◽  
Dental Pulp Stem Cells ◽  
Differentially Expressed ◽  
Expression Level

Abstract Background: Dental pulp stem cells (DPSCs) exhibited self-renewal, pluripotency capacity and served as promising cells source in endodontic regeneration and tissue engineering. Meanwhile, the regenerative capacity of DPSCs is limited and reduced in long lifespan. N6-methyladenosine (m6A) is the most prevalent, reversible internal modification in RNAs. The methyltransferases complex and demethylases mediated m6A methylation and cooperated to impact various biological processes associated with stem cell fate determination. However, the biological effect of m6A methylation in DPSCs remained unclear. Methods: Cell surface markers and differentiation potential of primary DPSCs were identified and m6A immunoprecipitation with deep sequencing (m6A RIP-seq) was used to uncover characteristics of m6A modifications in DPSCs transcriptome. Expression level of m6A-related genes were evaluated in immature/mature pulp tissues and cells. Lentiviral vectors were constructed to knockdown or overexpress methyltransferase like 3 (METTL3). Cell morphology, viability, senescence and apoptosis were further analyzed by β-galactosidase, TUNEL staining and flow cytometry. Bioinformatic analysis combing m6A RIP and shMETTL3 RNA-seq was used to functionally enrich overlapped genes and screen target of METTL3. Cell cycle distributions were assayed by flow cytometry and m6A RIP-qPCR was used to confirm METTL3 mediated m6A methylation in DPSCs. Results: Here, m6A peaks distribution, binding area and motif in DPSCs were first revealed by m6A RIP-seq. We also found a relative high expression level of METTL3 in immature DPSCs with superior regenerative potential and METTL3 knockdown induced cell apoptosis and senescence. Furthermore, Conjoint analysis of m6A RIP and RNA-sequencing showed differentially expressed genes affected by METTL3 depletion was mainly enriched in cell cycle, mitosis and alteration of METTL3 expression resulted in cell cycle arrest which indicated METTL3 make essential effect in cell cycle control. To further investigate underlying mechanisms, we explored proteins interaction network of differentially expressed genes and Polo-like Kinase 1 (PLK1), a critical cycle modulator was identified as target of METTL3-mediated m6A methylation in DPSCs. Conclusions: These results revealed m6A methylated hallmarks in DPSCs and a regulatory role of METTL3 in cell cycle control. Our study shed light on therapeutic approaches in vital pulp therapy and serve new insight in stem cells based tissue engineering.

Management of the compromised airway and role of tracheotomy in anaplastic thyroid carcinoma

Head & Neck ◽  
2015 ◽  
Vol 38 (1) ◽  
pp. 85-88 ◽  
Author(s):  
Navin Mani ◽  
Katherine McNamara ◽  
Natalie Lowe ◽  
Sean Loughran ◽  
Beng K. Yap
Keyword(s):  
Thyroid Carcinoma ◽  
Anaplastic Thyroid Carcinoma
Sign in / Sign up

Export Citation Format

Share Document