scholarly journals Tomato Seed Coat Permeability to Selected Carbon Nanomaterials and Enhancement of Germination and Seedling Growth

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Tatsiana A. Ratnikova ◽  
Ramakrishna Podila ◽  
Apparao M. Rao ◽  
Alan G. Taylor
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Seed Coat ◽  
Carbon Nanomaterials ◽  
Germination Percentage ◽  
Tomato Seed ◽  
Fluorescence And Electron Microscopy ◽  
Seedling Length ◽  
Early Seedling ◽  
Carbon Based Nanomaterials

Seed coat permeability was examined using a model that tested the effects of soaking tomato (Solanum lycopersicon) seeds in combination with carbon-based nanomaterials (CBNMs) and ultrasonic irradiation (US). Penetration of seed coats to the embryo by CBNMs, as well as CBNMs effects on seed germination and seedling growth, was examined. Two CBNMs, C60(OH)20(fullerol) and multiwalled nanotubes (MWNTs), were applied at 50 mg/L, and treatment exposure ranged from 0 to 60 minutes. Bright field, fluorescence, and electron microscopy and micro-Raman spectroscopy provided corroborating evidence that neither CBNM was able to penetrate the seed coat. The restriction of nanomaterial (NM) uptake was attributed to the semipermeable layer located at the innermost layer of the seed coat adjacent to the endosperm. Seed treatments using US at 30 or 60 minutes in the presence of MWNTs physically disrupted the seed coat; however, the integrity of the semipermeable layer was not impaired. The germination percentage and seedling length and weight were enhanced in the presence of MWNTs but were not altered by C60(OH)20. The combined exposure of seeds to NMs and US provided insight into the nanoparticle-seed interaction and may serve as a delivery system for enhancing seed germination and early seedling growth.

scholarly journals The Use of Germination Indices for Salinity Tolerance Classification of Pepper Cultivars

2019 ◽  
Vol 7 (9) ◽  
pp. 1304
Author(s):  
Gamze Kaya
Keyword(s):  
Salinity Tolerance ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Genotypic Variation ◽  
Germination Percentage ◽  
Control Treatment ◽  
Tolerance Index ◽  
Seedling Length ◽  
Early Seedling ◽  
Germination Indices

The study aimed to evaluate the use of germination indices as a screening tool for salinity tolerance during germination and early seedling growth of pepper cultivars, and to distinguish the potential for genetic responses to salt tolerance. In the study, the seeds of seven pepper cultivars were germinated at increasing NaCl levels of 5, 10, 15 and 20 dS/m and distilled water as the control treatment for 14 days. Germination percentage (GP), mean germination time (MGT), germination index (GI), germination stress tolerance index (GSTI), seedling length (SL), seedling fresh weight (SFW) and vigor index (VI) were investigated. Results showed that germination percentage decreased with increasing NaCl levels while the highest germination percentage at 20 dS/m was 92% in BT Burdem with no significant reduction. Seedling growth of pepper cultivars was severely inhibited by increasing salinity stress. SFW was depressed depending on reduction in SL due to increasing NaCl. BT-Burli and BT İnce Sivri were the most tolerant cultivars to NaCl and they were used for genetic resources towards salinity. Seedling growth was much more sensitive to salinity than germination because of the highest percent reduction in seedling growth parameters. Among the parameters, GSTI gave the highest significant correlation coefficient with SL and SFW; indicating that it would be useful for estimating seedling growth. It was concluded that genotypic variation was observed among pepper cultivars for salinity tolerance and GSTI could be used for a predictor for salinity tolerance.

Effect of heavy metal stress on emerging plants community constructions in wetland

2010 ◽  
Vol 62 (10) ◽  
pp. 2459-2466 ◽  
Author(s):  
Han Peng ◽  
Wu Geng ◽  
Wu Yong-quan ◽  
Li Mao-teng ◽  
Xiang Jun ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Seed Germination ◽  
Seedling Growth ◽  
Heavy Metal Stress ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Wetland Ecosystem ◽  
Ecological Thresholds ◽  
Hongze Lake ◽  
Seedling Length

In this paper, we report the effects of heavy metals (HMs) (cadmium and mercury) on seed germination and seedling growth of Phragmites australis and Triarrhena sacchariflora, which are the two main typical emerging plants in Hongze Lake wetland. The results showed that there was a reduction in germination percentage, germination index and seedling length as HM concentration in the growing media increased for both treatments. The effect of HMs toxicity on seed germination and seedling growth of T. sacchariflora was more obvious than of P. australis. At the stage of seed germination, P. australis and T. sacchariflora were sensitive to Hg2 +  and Cd2 + , respectively, and Hg2 +  was more toxic than Cd2 +  at the stage of seedling growth. The effect of HMs toxicity is not invariable during plant growth. Compared to the stage of seedling growth, P. australis and T. sacchariflora are more susceptible to HMs at the stage of seed germination. In addition, we calculated the ecological thresholds of P. australis to Cd and Hg are 19.32 and 1.08 mg kg−1, and that of T. sacchariflora to Cd is 4.62 mg kg−1 based on the lab simulation. The results also indicated that the species of P. australis is more tolerant than T. sacchariflora to the HMs and is a better candidate for restoration in Hongze Lake wetland ecosystem.

scholarly journals Differences in the Seed Germination of Leymus chinensis (Poaceae) Ecotypes Reveal Distinct Strategies for Coping With Salinity Stress: A Common Garden Experiment

2021 ◽  
Vol 9 ◽  
Author(s):  
Meng-Yao Ma ◽  
Hong-Yuan Ma ◽  
Lei Wang ◽  
Wen-Wen Qi ◽  
Shao-Yang Li ◽  
...  
Keyword(s):  
Seed Germination ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Common Garden ◽  
Nacl Stress ◽  
Germination Percentage ◽  
Leymus Chinensis ◽  
Seedling Length ◽  
Mean Germination Time ◽  
Total Seed

Soil salinity is important abiotic stress affecting various ecosystems worldwide such as grassland. Distinct ecotypes often evolve within species by natural selection to facilitate adaptation to different types of environmental stress. Leymus chinensis is a perennial rhizomatous grass that is widely distributed in the eastern Eurasian steppe; it has two main ecotypes, namely, yellow-green (YG) and gray-green (GG), which differ in their strategy for coping with salinity stress. Few studies have examined the seed germination of the two ecotypes under salinity stress. In this study, the seed germination and seedling growth of two ecotypes of L. chinensis in response to different levels of salinity (NaCl) stress [0 (control), 20, 50, 100, and 200 mM] were examined. Then, ungerminated seeds were placed under normal conditions to evaluate seedling growth following exposure to salt stress (i.e., regermination). The germination percentage was significantly higher, and the mean germination time was significantly shorter in the GG ecotype than in the YG ecotype at all NaCl concentrations. As the salinity level increased, the radicle length of the two ecotypes decreased; however, GG had longer radicles and a higher number of radicles, even at 200 mM NaCl when no radicle protruding from the seed coat was detected in YG. The shoot length of GG was significantly longer than that of YG at all NaCl levels. After salinity stress was removed, the seed germination percentage increased as the original concentration of NaCl applied increased, but the total germination percentage did not significantly differ among NaCl concentrations. The total seed germination percentage of GG was approximately 80%, whereas that of the YG was approximately 20%. The seedling length of regerminated seeds for both GG and YG was similar. The thousand-grain weight of GG was significantly higher than that of YG. GG was more salt-tolerant than YG and might be better capable of surviving in harsher environments, suggesting that GG might be particularly useful for saline grassland restoration.

scholarly journals Seed priming with salicylic acid potentiates water restriction-induced effects in tomato seed germination and early seedling growth

2020 ◽  
Vol 42 ◽  
Author(s):  
Yutcelia Carolina Galviz-Fajardo ◽  
Gabriel Streck Bortolin ◽  
Sidnei Deuner ◽  
Luciano do Amarante ◽  
Fernanda Reolon ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Salicylic Acid ◽  
Seed Germination ◽  
Seedling Growth ◽  
Seed Priming ◽  
Water Restriction ◽  
Tomato Seed ◽  
Early Seedling Growth ◽  
Germination Speed ◽  
Early Seedling

Abstract: Seed priming with salicylic acid (SA) has been described as a method with potential to improve seed germination under unfavorable conditions. The present study aimed to evaluate the ability of seed priming with SA to attenuate the impact of water restriction during germination and early seedling growth of tomato. Seeds were subjected to different concentrations of SA (0, 25 and 50 µM) and sowed on substrates at different percentages of the water holding capacity (20, 30, 40 and 100%). The following variables were evaluated: germination, first count of germination, germination speed index, shoots/root length and dry mass, superoxide dismutase, ascorbate peroxidase and catalase activities, contents of hydrogen peroxide and of malondialdehyde. Water restriction significantly decreased the variables related to germination and seedling performance. Despite increasing germination speed and seedling development when combined with well-irrigated conditions, priming with SA negatively impacted those characteristics under water deficit conditions. That was through increases in superoxide dismutase activity and H2O2 accumulation. Thus, it was concluded that seed priming with SA does not improve tomato seed germination and early seedling growth under water deficit.

scholarly journals Basal Transcription Factor 3 Plays an Important Role in Seed Germination and Seedling Growth of Rice

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Wenyi Wang ◽  
Mengyun Xu ◽  
Ya Wang ◽  
Muhammad Jamil
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Morphological Changes ◽  
Germination Percentage ◽  
Seedling Stage ◽  
Wild Type ◽  
Conserved Domain ◽  
Transgenic Lines ◽  
Early Seedling ◽  
Regulatory Functions

BTF3 has been recognized to be involved in plant growth and development. But its function remains mostly unknown during seed germination and seedling stage. Here, we have analyzed OsBTF3-related sequences inOryza sativaL. subspecies, japonica, which resembles with the conserved domain of a nascent polypeptide associated complex (NAC) with different homologs of OsBTF3 and human BTF3. Inhibition ofOsj10gBTF3has led to considerable morphological changes during seed germination and seedling growth. Germination percentage was not influenced by the application of GA3, ABA, and NaCl but all concentrations caused wild-type (WT) seeds to germinate more rapidly than the RNAi (Osj10gBTF3Ri) transgenic lines. Seedling inhibition was more severe in theOsj10gBTF3Riseedlings compared with their WT especially when treated with 100 or 200 μM GA3; 50% reduction in shoots was observed inOsj10gBTF3Riseedlings. The expression ofOsj3g1BTF3,Osj3g2BTF3andOsj10gBTF3was primarily constitutive and generally modulated by NaCl, ABA, and GA3stresses in bothOsj10gBTF3Rilines and WT at the early seedling stage, suggesting thatOsj3g1BTF3andOsj10gBTF3are much similar but different fromOsj3g2BTF3in biological function. These results show that OsBTF3 plays an important role in seed germination and seedling growth gives a new perception demonstrating that more multifaceted regulatory functions are linked with BTF3 in plants.

scholarly journals Allelopathic Effect of Echinochloa colona L. and Cyperus iria L. Weed Extracts on the Seed Germination and Seedling Growth of Rice and Soyabean

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Neha Chopra ◽  
Geeta Tewari ◽  
Lalit M. Tewari ◽  
Brij Upreti ◽  
Naveen Pandey
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Aerial Part ◽  
Water Soluble ◽  
Allelopathic Effect ◽  
Root Extract ◽  
Allelopathic Potential ◽  
Root Extracts ◽  
Seedling Length ◽  
Early Seedling

The present study was undertaken to assess the allelopathic effect of Echinochloa colona L. and Cyperus iria L. in relation to the germination and primary growth of Oryza sativa L. (rice) and Glycine max L. (soyabean). Effects of dichloromethane (DCM) and double distilled water soluble (DDW) fractions of E. colona L. and C. iria L. root and aerial part extracts reduced germination and suppressed early seedling growth of rice and soyabean. With increase in extract concentration from 1 to 100 mg/mL, a gradual decrease in seed germination and seedling length occurred. The highest growth of G. max seedling was recorded in DDW fraction of E. colona aerial part extract at 1 mg/mL concentration with 94% germination and the lowest length was found in DCM fraction of C. iria root extract at 100 mg/mL concentration with 65% germination. In O. sativa, the highest length was noted at 1 mg/mL concentration in DDW fraction of E. colona aerial part extract with 82% germination and the lowest length was found in DCM fraction of C. iria and E. colona root extracts with germination 57% and 62%, respectively, at 100 mg/mL concentration. The results suggested that these weeds had good allelopathic potential which reduces germination and plant growth.

scholarly journals AUTOTOXICITY IN TOUGH LOVEGRASS ( Eragrostis plana )

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
A. FAVARETTO ◽  
S.M. SCHEFFER-BASSO ◽  
N.B. PEREZ
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Germination Percentage ◽  
Shoot Length ◽  
Aqueous Extracts ◽  
Early Seedling Growth ◽  
Germination Speed ◽  
Early Seedling ◽  
Mean Germination Time ◽  
Growth Of Seedlings

ABSTRACT Autotoxicity in tough lovegrass has been analyzed in the field, but has never been tested in allelopathy bioassays. Therefore, this study aimed to verify and compare the autotoxicity of leaves and roots from aqueous extracts of tough lovegrass on its germination and early seedling growth. Extracts of green leaves (GL), senescent leaves (SL), and roots (R) at concentrations of 0.05, 0.15, and 0.25 g mL-1 were analyzed on how they affected seed germination and seedling growth. The extracts, regardless of the source or concentration of the material used, have significantly reduced germination percentage, index of germination speed, and root growth of seedlings of tough lovegrass. Effects on mean germination time and shoot length varied according to the origin and concentration of the extracts. GL, at concentrations 0.15 and 0.25 g mL-1, showed the most damaging effect. In tough lovegrass, autotoxicity is a mechanism that may be involved in the control of germination and plant growth, which could explain the distance between their clumps in the field.

scholarly journals Effects of Calcium Alginate Submicroparticles on Seed Germination and Seedling Growth of Wheat (Triticum aestivum L.)

Polymers ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1154 ◽  
Author(s):  
Jinxia He ◽  
Ruixin Li ◽  
Xue Sun ◽  
Wenxia Wang ◽  
Jianen Hu ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Soluble Protein ◽  
Calcium Alginate ◽  
Adventitious Roots ◽  
Average Diameter ◽  
Triticum Aestivum L ◽  
Germination Percentage ◽  
Wheat Roots ◽  
Seedling Length

Calcium alginate (CaAlg) submicroparticles have a potential application in agricultural delivery systems. This study investigated the effects of CaAlg submicroparticles on seed germination and seedling growth of wheat. CaAlg submicroparticles with a Z-average diameter of around 250.4 nm and a measured zeta potential value of about −25.4 mV were prepared and characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometer (EDS). After this, the effects of the concentration of CaAlg submicroparticles (10–500 μg/mL) on germination percentage, seedling length, the number of adventitious roots, chlorophyll content and soluble protein content were evaluated. The results demonstrated a significant increase in the level of germination percentage (9.0%), seedling index (50.3%), adventitious roots (27.5%), seedling length (17.0%), chlorophyll (8.7%) and soluble protein contents (4.5%) at a concentration of 100 μg/mL. However, an inhibitory effect was observed at a concentration of 500 μg/mL. The SEM examination showed that CaAlg submicroparticles could be successfully adsorbed onto the surface of the wheat seed. Further studies proved that CaAlg submicroparticles at a concentration of 100 μg/mL promoted the expression of indole-3-acetic acid (IAA)-related genes (YUCCA9, AUX1, ARF and UGT) in wheat, which resulted in an increase of 69% and 21% in IAA concentration in wheat roots and shoots, respectively.

scholarly journals Autoallelopathic Potential of Leaflets and Seeds on Seedling Growth of Date Palm (Phoenix dactylifera L.)

2000 ◽  
Vol 5 (1) ◽  
pp. 7 ◽  
Author(s):  
M.O.A. Warrag ◽  
A.S. AI-Wasel
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Date Palm ◽  
Germination Rate ◽  
Phoenix Dactylifera ◽  
Germination Percentage ◽  
Water Soluble ◽  
Water Control ◽  
Early Seedling Growth ◽  
Early Seedling

The autoalle1opathic effects of date palm leaflets and seed aqueous extracts, with -0.05, -0.1 and -0.2 mPa osmotic potentials, on seed germination and early seedling growth were investigated. Final seed germination percentage, seed germination rate, the time of radicle appearance, and plumule length were not affected. In contrast, the cotyledonary sheath and radicle lengths were significantly retarded, whereas the time of plumule appearance was significantly increased by most of the extracts in comparison with the distilled water control. Mannitol solutions, with the same osmotic potential and pH as the extracts, resulted in significantly longer cotyledonary sheaths and radicles and an earlier plumule appearance than their corresponding extracts. Thus, it could be deduced that date palm leaflets and seeds contain water-soluble allelochemicals that could substantially retard early seedling growth of the same species.

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