scholarly journals Multidrug Resistance inEscherichia coliStrains Isolated from Infections in Dogs and Cats in Poland (2007–2013)

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Magdalena Rzewuska ◽  
Michał Czopowicz ◽  
Magdalena Kizerwetter-Świda ◽  
Dorota Chrobak ◽  
Borys Błaszczak ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Antimicrobial Susceptibility ◽  
Continuous Monitoring ◽  
Companion Animals ◽  
Small Animal ◽  
Multidrug Resistant ◽  
Appropriate Use ◽  
E Coli ◽  
Extended Spectrum

The antimicrobial susceptibility ofEscherichia coliisolates associated with various types of infections in dogs and cats was determined. The studied isolates were most frequently susceptible to fluoroquinolones and the extended-spectrum cephalosporins (ESCs), antimicrobials commonly used in treatment of infections in companion animals. However, an increase in the percentage of strains resistant toβ-lactam antibiotics including ESCs was noted between January 2007 and December 2013. The frequency of multidrug-resistant (MDR)E. coliisolation (66.8% of isolates) is alarming. Moreover, the statistically significant increase of the percentage of MDR isolates was observed during the study period. No difference in the prevalence of multidrug resistance was found between bacteria causing intestinal and extraintestinal infections and between canine and feline isolates. NonhemolyticE. coliisolates were MDR more often than hemolytic ones. Our study showed the companion animals in Poland as an important reservoir of MDR bacteria. These results indicate that continuous monitoring of canine and felineE. coliantimicrobial susceptibility is required. Furthermore, introduction and application of recommendations for appropriate use of antimicrobials in small animal practice should be essential to minimize the emergence of multidrug resistance amongE. coliin companion animals.

scholarly journals Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Isolated from Flies in the Urban Center of Berlin, Germany

2019 ◽  
Vol 16 (9) ◽  
pp. 1530 ◽  
Author(s):  
Wibke Wetzker ◽  
Yvonne Pfeifer ◽  
Solvy Wolke ◽  
Andrea Haselbeck ◽  
Rasmus Leistner ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Continuous Monitoring ◽  
Companion Animals ◽  
Multidrug Resistant ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Ciprofloxacin Resistance ◽  
Multiple Pathogens

Background: The monitoring of antimicrobial resistance (AMR) in microorganisms that circulate in the environment is an important topic of scientific research and contributes to the development of action plans to combat the spread of multidrug-resistant (MDR) bacteria. As a synanthropic vector for multiple pathogens and a reservoir for AMR, flies can be used for surveillance. Methods: We collected 163 flies in the inner city of Berlin and examined them for extended-spectrum β-lactamase (ESBL)-producing Escherichia coli genotypically and phenotypically. Results: The prevalence of ESBL-producing E. coli in flies was 12.9%. Almost half (47.6%) of the ESBL-positive samples showed a co-resistance to ciprofloxacin. Resistance to carbapenems or colistin was not detected. The predominant ESBL-type was CTX-M-1, which is associated with wildlife, livestock, and companion animals as a potential major source of transmission of MDR E. coli to flies. Conclusions: This field study confirms the permanent presence of ESBL-producing E. coli in an urban fly population. For continuous monitoring of environmental contamination with multidrug-resistant (MDR) bacteria, flies can be used as indicators without much effort.

scholarly journals Antimicrobial Susceptibility Profiles of Escherichia coli Isolates from Diarrheic Dogs in Maiduguri, Borno State, Nigeria

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Muhammad Mustapha ◽  
Yusuf Audu ◽  
Kingsley Uwakwe Ezema ◽  
Jafar Umar Abdulkadir ◽  
Jallailudeen Rabana Lawal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Companion Animals ◽  
Small Animal ◽  
Multidrug Resistant ◽  
Biochemical Tests ◽  
Antibiotic Susceptibility Pattern ◽  
E Coli ◽  
Intestinal Infections ◽  
Susceptibility Profiles

AbstractDiarrhea caused by multidrug-resistant Escherichia coli (E. coli) is an important and common problem in companion animals, especially dogs. Moreover, these dogs may serve as a reservoir of pathogenic strains of E. coli that may cause enteric and extra-intestinal infections in humans and other animals. This study was conducted to investigate the antibiotic susceptibility pattern of E. coli isolates from diarrheic dogs in Maiduguri Metropolis, Borno State, Nigeria. In fecal samples of 200 dogs with diarrhea, 147 E. coli strains (73.5%) were isolated and characterized by the standard bacteriological techniques (culture, biochemical tests, and antimicrobial susceptibility testing). Out of the 147 positive isolates, 45, 50, and 52 were from Elkanemi Park, Magaram, and Sabon gari wards respectively. The isolates show 100% resistance to chloramphenicol, cefuroxime, and ceftriaxone, 96.6% to amoxicillin, and 95.9% to gentamicin, while all (100%) were susceptible to ciprofloxacin. All the isolates showed multiple antimicrobial resistance. The result of the current study showed that dogs in Maiduguri are important reservoirs of multidrug-resistant E. coli. Therefore, it is important to adopt and apply guidelines for the correct use of antimicrobials in small animal practice to reduce the emergence of multidrug resistance among E. coli in companion animals.

scholarly journals Characterization of Extended-Spectrum-β-Lactamases Produced by Escherichia coli Strains Isolated from Dogs in Poland

2015 ◽  
Vol 64 (3) ◽  
pp. 285-288 ◽  
Author(s):  
Magdalena Rzewuska ◽  
Ilona Stefańska ◽  
Magdalena Kizerwetter-Świda ◽  
Dorota Chrobak-Chmiel ◽  
Paulina Szczygielska ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Companion Animals ◽  
Small Animal ◽  
Common Cause ◽  
First Report ◽  
E Coli ◽  
Veterinary Practice ◽  
Extended Spectrum

Escherichia coli is a common cause of infections in companion animals. In recent years the increasing prevalence of resistance to β-lactams, including extended-spectrum cephalosporins, antimicrobials frequently used in small animal veterinary practice, was observed in canine isolates of E. coli. The aim of this study was to detect and to characterize extended-spectrum β-lactamases (ESBLs) produced by E. coli isolated from diseased dogs in Poland. Four isolates out of 119 studied (3.4%) were ESBL-positive. They harbored the blaSHV-12, blaCTX-M-15, and blaTEM-116 genes. This study provides the first report of the occurrence of ESBL-producing E. coli in dogs in Poland.

scholarly journals Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Abdulaziz Alqasim ◽  
Ahmad Abu Jaffal ◽  
Abdullah A. Alyousef
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Antimicrobial Susceptibility ◽  
Empirical Therapy ◽  
E Coli ◽  
Extended Spectrum ◽  
High Prevalence ◽  
Tertiary Healthcare ◽  
Polymerase Chain ◽  
Synergy Test

The prevalence of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-producing E. coli) has recently increased worldwide. This study aims at determining the antimicrobial susceptibility patterns of a collection of clinical E. coli urine isolates and evaluating the ESBL carriage of these isolates at phenotypic and genotypic levels. A total of 100 E. coli urine isolates were collected at a tertiary healthcare centre in Riyadh from January 2018 to March 2018. Antimicrobial susceptibility testing was carried out for all isolates. ESBL production was characterized at phenotypic and genotypic levels using double-disc synergy test and polymerase chain reaction, respectively. Detection of different ESBL variants was performed using DNA sequencing. Of 100 E. coli isolates, 67 were associated with multidrug resistance (MDR) phenotype. All isolates showed variable resistance levels to all antibiotics used here expect to imipenem, where they were all imipenem-sensitive. 33 out of 100 E. coli isolates were positive for ESBLs by phenotypic and genotypic methods. Among all ESBL-positive E. coli isolates, the CTX-M was the most prevalent ESBL type (31/33 isolates; 93.94%). CTX-M-15 variant was detected in all isolates associated with CTX-M carriage. Multiple ESBL gene carriage was detected in 15/33 isolates (45.45%), where 11 (33.33%) isolates produced two different ESBL types while 4 isolates (12.12%) associated with carrying three different ESBL types. Our study documented the high antimicrobial resistance of ESBL-producing E. coli to many front-line antibiotics currently used to treat UTI patients, and this implies the need to continuously revise the local guidelines used for optimal empirical therapy for UTI patients. It also showed the high prevalence of ESBL carriage in E. coli urine isolates, with CTX-M-15 being the most predominant CTX-M variant.

scholarly journals Escherichia coli O15 : H7 from Food of Animal Origin in Arsi: Occurrence at Catering Establishments and Antimicrobial Susceptibility Profile

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Geresu Minda Asfaw ◽  
Regassa Shimelis
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Antimicrobial Susceptibility ◽  
Public Health Problem ◽  
Raw Milk ◽  
Multidrug Resistant ◽  
Animal Origin ◽  
E Coli ◽  
Food Of Animal Origin ◽  
Antimicrobial Susceptibility Profile

Escherichia coli O157 : H7 (E. coli O157 : H7) has been found to be the major cause of food-borne diseases and a serious public health problem in the world, with an increasing concern for the emergence and spread of antimicrobial-resistant strains. Hitherto, little is known about the carriage of E. coli O157 : H7 and its antimicrobial susceptibility profile in the food of animal origin in Ethiopia. This study aimed to determine the occurrence and multidrug resistance profile of E. coli O157 : H7 from food of animal origin at different catering establishments in the selected study settings of Arsi Zone. One hundred ninety-two animal origin food items, namely, raw/minced meat (locally known as “Kitfo,” “Kurt,” and “Dulet”), raw milk, egg sandwich, and cream cake samples were collected and processed for microbiological detection of E. coli O157 : H7. Out of 192 samples, 2.1% (4/192) were positive for E. coli O157 : H7. Two E. coli O157 : H7 isolates were obtained from “Dulet” (6.3%) followed by “Kurt” (3.1%, 1/32) and raw milk (3.1%, 1/32), whereas no isolate was obtained from “Kitfo,” egg sandwich, and cream cake samples. Of the 4 E. coli O157 : H7 isolates subjected to 10 panels of antimicrobial discs, 3 (75%) were highly resistant to kanamycin, streptomycin, and nitrofurantoin. Besides, all the isolates displayed multidrug resistance phenotypes, 3 to 5 antimicrobial resistance, amid kanamycin, streptomycin, nitrofurantoin, tetracycline, and chloramphenicol. The occurrence of multidrug-resistant E. coli O157 : H7 isolates from foods of animal origin sampled from different catering establishments reveals that the general sanitary condition of the catering establishments, utensils used, and personnel hygienic practices did not comply with the recommended standards. Thus, this finding calls for urgent attention toward appropriate controls and good hygienic practices in different catering establishments dealing with consuming raw/undercooked foods of animal origin.

scholarly journals Analysis of Multidrug Resistance Profile of Escherichia coli from Clinical Samples from Companion Animals and Bird Retrospect to Five-year (2015-2019) Literature Data

2021 ◽  
Vol 11 (5) ◽  
pp. 12506-12515
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Diarrheal Disease ◽  
Companion Animals ◽  
Multidrug Resistant ◽  
Clinical Samples ◽  
Group B Streptococci ◽  
Indirect Contact ◽  
E Coli ◽  
Escherichia Coli Infection

Based on WHO 2017 data of death rate among the children of aging under five years, a diarrheal disease in post-neonates and sepsis in neonate posse second and third major concern, respectively. Though Group B Streptococci infection is a primary etiological agent, Escherichia coli infection is the major cause of mortality. Multidrug resistance in E. coli was studied from companion animals, addressing zoonotic disease transfer possibilities to their handler or in the community via direct or indirect contact or through contaminated food or water. Out of 100 samples cultured, 78 bacterial pathogens were isolated, from which 29 (38%) isolates were E. coli, identified using IMViC and confirmed by Vitek2. Antibiotic susceptibility against 42 antibiotics belonging to 12 different antimicrobial categories was performed by the Kirby-Bauer method of disk diffusion assay. By using WHONET software, an antibiogram was deduced and found that 23 (80%) isolates were multidrug-resistant (MDR) and 4 (13%) were possible extensively drug-resistant (possible XDR). Comparison of resistance data to the literature data of the period 2015-2019 supplemented with details of susceptibility either in the form of disc diffusion test or minimum inhibitory concentration (MIC) was carried out to understand the current scenario of drug resistance in E. coli of non-human origin.

scholarly journals Phenotypic Characterization of Multidrug-resistant Escherichia Coli with Special Reference to Extended-spectrum-beta-lactamases and Metallo-beta-lactamases in a Tertiary Care Center

2015 ◽  
Vol 53 (198) ◽  
pp. 83-88 ◽  
Author(s):  
Basudha Shrestha ◽  
Shovita Shrestha ◽  
Shyam Kumar Mishra ◽  
Hari Prasad Kattel ◽  
Tatsuya Tada ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Resistance Mechanism ◽  
Multidrug Resistant ◽  
Confirmatory Test ◽  
Beta Lactamase ◽  
Beta Lactamases ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

Introduction: The increasing reports on extended-spectrum-beta-lactamase and metallo-betalactamase producing Escherichia coli have addressed a potential threat to global health since it is found to be highly resistance to most of the currently available antibiotics including carbapenems. The present study was aimed to determine the antibiogram of extended-spectrum-beta-lactamase and metallo-beta-lactamase producing MDR E. coli isolates from various clinical samples. Methods: This was a cross-sectional study conducted over a period of seven months (December 2013 to July 2014) at bacteriology laboratory of Tribhuvan University Teaching Hospital. A total of 250 clinical specimens (urine, pus, sputum, blood, body fluid, bile, tissue and central venous pressure line tip) were processed from inpatients, with multidrug-resistant Escherichia coli infections. Standard microbiological techniques were used for isolation and identification of the isolates. The presence of extended-spectrum-beta-lactamase was detected by phenotypic confirmatory test recommended by Clinical and Laboratory Standards Institute and imipenem (IMP) /EDTA combined disc method was performed to detect metallo-beta-lactamase mediated resistance mechanism. Results: We found high level of beta lactamase mediated resistance mechanism as part of multidrug resistance. Among 250 MDR isolates, 60% isolates were extended-spectrum-beta-lactamase producers and 17.2% isolates were metallo-beta-lactamase producers. Co-existence of extended-spectrum-betalactamase and metallo-beta-lactamase identified in 6.8% isolates. Conclusions: Beta-lactamase mediated resistance mechanisms are accounting very high in the multidrug resistant isolates of E. coli. Therefore, early detection of beta lactamase mediated resistant strains and their current antibiotic susceptibility pattern is necessary to avoid treatment failure and prevent the spread of MDR.  Keywords: e. coli; extended-spectrum-β-lactamase; metallo-β-lactamase; multidrug-resistance.

scholarly journals Antimicrobial Resistance Profiling and Molecular Epidemiological Analysis of Extended Spectrum β-Lactamases Produced by Extraintestinal Invasive Escherichia coli Isolates From Ethiopia: The Presence of International High-Risk Clones ST131 and ST410 Revealed

2021 ◽  
Vol 12 ◽  
Author(s):  
Abebe Aseffa Negeri ◽  
Hassen Mamo ◽  
Jyoti M. Gurung ◽  
A. K. M. Firoj Mahmud ◽  
Maria Fällman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
High Risk ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Bacterial Typing ◽  
E Coli ◽  
Extended Spectrum ◽  
Genes Encoding

The treatment of invasive Escherichia coli infections is a challenge because of the emergence and rapid spread of multidrug resistant strains. Particular problems are those strains that produce extended spectrum β-lactamases (ESBL’s). Although the global characterization of these enzymes is advanced, knowledge of their molecular basis among clinical E. coli isolates in Ethiopia is extremely limited. This study intends to address this knowledge gap. The study combines antimicrobial resistance profiling and molecular epidemiology of ESBL genes among 204 E. coli clinical isolates collected from patient urine, blood, and pus at four geographically distinct health facilities in Ethiopia. All isolates exhibited multidrug resistance, with extensive resistance to ampicillin and first to fourth line generation cephalosporins and sulfamethoxazole-trimethoprim and ciprofloxacin. Extended spectrum β-lactamase genes were detected in 189 strains, and all but one were positive for CTX-Ms β-lactamases. Genes encoding for the group-1 CTX-Ms enzymes were most prolific, and CTX-M-15 was the most common ESBL identified. Group-9 CTX-Ms including CTX-M-14 and CTX-27 were detected only in 12 isolates and SHV ESBL types were identified in just 8 isolates. Bacterial typing revealed a high amount of strains associated with the B2 phylogenetic group. Crucially, the international high risk clones ST131 and ST410 were among the sequence types identified. This first time study revealed a high prevalence of CTX-M type ESBL’s circulating among E. coli clinical isolates in Ethiopia. Critically, they are associated with multidrug resistance phenotypes and high-risk clones first characterized in other parts of the world.

scholarly journals Multidrug-Resistant, Including Extended-Spectrum Beta Lactamase-Producing and Quinolone-Resistant, Escherichia coli Isolated from Poultry and Domestic Pigs in Dar es Salaam, Tanzania

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 406
Author(s):  
Zuhura I. Kimera ◽  
Fauster X. Mgaya ◽  
Gerald Misinzo ◽  
Stephen E. Mshana ◽  
Nyambura Moremi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Antibiotics Resistance ◽  
Beta Lactamase ◽  
Domestic Pigs ◽  
Extended Spectrum Beta Lactamase ◽  
Phenotypic Profile ◽  
E Coli ◽  
Extended Spectrum ◽  
Esbl Producers

We determined the phenotypic profile of multidrug-resistant (MDR) Escherichia coli isolated from 698 samples (390 and 308 from poultry and domestic pigs, respectively). In total, 562 Enterobacteria were isolated. About 80.5% of the isolates were E. coli. Occurrence of E. coli was significantly higher among domestic pigs (73.1%) than in poultry (60.5%) (p = 0.000). In both poultry and domestic pigs, E. coli isolates were highly resistant to tetracycline (63.5%), nalidixic acid (53.7%), ampicillin (52.3%), and trimethoprim/sulfamethoxazole (50.9%). About 51.6%, 65.3%, and 53.7% of E. coli were MDR, extended-spectrum beta lactamase-producing enterobacteriaceae (ESBL-PE), and quinolone-resistant, respectively. A total of 68% of the extended-spectrum beta lactamase (ESBL) producers were also resistant to quinolones. For all tested antibiotics, resistance was significantly higher in ESBL-producing and quinolone-resistant isolates than the non-ESBL producers and non-quinolone-resistant E. coli. Eight isolates were resistant to eight classes of antimicrobials. We compared phenotypic with genotypic results of 20 MDR E. coli isolates, ESBL producers, and quinolone-resistant strains and found 80% harbored blaCTX-M, 15% aac(6)-lb-cr, 10% qnrB, and 5% qepA. None harbored TEM, SHV, qnrA, qnrS, qnrC, or qnrD. The observed pattern and level of resistance render this portfolio of antibiotics ineffective for their intended use.

scholarly journals Molecular survey of mcr1 and mcr2 plasmid mediated colistin resistance genes in Escherichia coli isolates of animal origin in Iran

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Kayhan Ilbeigi ◽  
Mahdi Askari Badouei ◽  
Hossein Vaezi ◽  
Hassan Zaheri ◽  
Sina Aghasharif ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Companion Animals ◽  
Multidrug Resistant ◽  
Animal Origin ◽  
Colistin Resistance ◽  
E Coli ◽  
High Level ◽  
Farming Industry

Abstract Objectives The emergence of colistin-resistant Enterobacteriaceae from human and animal sources is one of the major public health concerns as colistin is the last-resort antibiotic for treating infections caused by multidrug-resistant Gram-negative bacteria. We aimed to determine the prevalence of the prototype widespread colistin resistance genes (mcr-1 and mcr-2) among commensal and pathogenic Escherichia coli strains isolated from food-producing and companion animals in Iran. Results A total of 607 E. coli isolates which were previously collected from different animal sources between 2008 and 2016 used to uncover the possible presence of plasmid-mediated colistin resistance genes (mcr-1 and mcr-2) by PCR. Overall, our results could not confirm the presence of any mcr-1 or mcr-2 positive E. coli among the studied isolates. It is concluded that despite the important role of food-producing animals in transferring the antibiotic resistance, they were not the main source for carriage of mcr-1 and mcr-2 in Iran until 2016. This study suggests that the other mcr variants (mcr-3 to mcr-9) might be responsible for conferring colistin resistance in animal isolates in Iran. The possible linkage between pig farming industry and high level of mcr carriage in some countries needs to be clarified in future prospective studies.

Sign in / Sign up

Export Citation Format

Share Document