scholarly journals Single Whole-Body Cryostimulation Procedure versus Single Dry Sauna Bath: Comparison of Oxidative Impact on Healthy Male Volunteers

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Paweł Sutkowy ◽  
Alina Woźniak ◽  
Paweł Rajewski
Keyword(s):  
Thiobarbituric Acid ◽  
Whole Body ◽  
Human Organism ◽  
Healthy Male ◽  
Thiobarbituric Acid Reactive Substances ◽  
Sod Activity ◽  
High And Low Temperatures ◽  
The Impact ◽  
Environmental Temperatures ◽  
Male Subjects

Exposure to extreme heat and cold is one of the environmental factors whose action is precisely based on the mechanisms involving free radicals. Fluctuations in ambient temperature are among the agents that toughen the human organism. The goal of the study was to evaluate the impact of extremely high (dry sauna, DS) and low (whole-body cryostimulation, WBC) environmental temperatures on the oxidant-antioxidant equilibrium in the blood of healthy male subjects. The subjects performed a single DS bath (n=10; 26.2 ± 4.6 years) and a single WBC procedure (n=15; 27.5 ± 3.1 years). In the subjects’ blood taken immediately before and 20 min after the interventions, the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and the concentration of thiobarbituric acid reactive substances in erythrocytes (TBARSer) and blood plasma (TBARSpl) were determined. Single WBC and DS procedures induced an increase in the activity of SOD and GPx, as well as SOD and CAT, respectively. The SOD activity was higher after WBC than after DS. Extremely high and low temperatures probably induce the formation of reactive oxygen species in the organisms of healthy men and, therefore, disturb the oxidant-antioxidant balance.

scholarly journals Buprenorphine and Methadone as Opioid Maintenance Treatments for Heroin-Addicted Patients Induce Oxidative Stress in Blood

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Christonikos Leventelis ◽  
Nikolaos Goutzourelas ◽  
Aikaterini Kortsinidou ◽  
Ypatios Spanidis ◽  
Georgia Toulia ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Redox Status ◽  
Protein Carbonyls ◽  
Healthy Individuals ◽  
Thiobarbituric Acid Reactive Substances ◽  
Antioxidant Defence ◽  
Total Antioxidant ◽  
Beneficial Action ◽  
The Impact

Buprenorphine and methadone are two substances widely used in the substitution treatment of patients who are addicted to opioids. Although it is known that they partly act efficiently towards this direction, there is no evidence regarding their effects on the redox status of patients, a mechanism that could potentially improve their action. Therefore, the aim of the present investigation was to examine the impact of buprenorphine and methadone, which are administered as substitutes to heroin-dependent patients on specific redox biomarkers in the blood. From the results obtained, both the buprenorphine (n=21) and the methadone (n=21) groups exhibited oxidative stress and compromised antioxidant defence. This was evident by the decreased glutathione (GSH) concentration and catalase activity in erythrocytes and the increased concentrations of thiobarbituric acid reactive substances (TBARS) and protein carbonyls in the plasma, while there was no significant alteration of plasma total antioxidant capacity (TAC) compared to the healthy individuals (n=29). Furthermore, methadone revealed more severe oxidant action compared to buprenorphine. Based on relevant studies, the tested substitutes mitigate the detrimental effects of heroin on patient redox status; still it appears that they need to be boosted. Therefore, concomitant antioxidant administration could potentially enhance their beneficial action, and most probably, buprenorphine that did not induce oxidative stress in such a severe mode as methadone, on the regulation of blood redox status.

Assessment of quality and clinical similarity (pharmacokinetic and safety) of HD204, a biosimilar of bevacizumab.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e21556-e21556
Author(s):  
Jocelyn Hii ◽  
Martin Demarchi ◽  
Pierre Coliat ◽  
Michael J Kim ◽  
Kuo Wei Chan ◽  
...  
Keyword(s):  
Clinical Study ◽  
Cancer Progression ◽  
Neutralizing Antibodies ◽  
Volume Of Distribution ◽  
Prospective Clinical Study ◽  
Healthy Male ◽  
Clinical Phase ◽  
Humanized Monoclonal Antibody ◽  
The Impact ◽  
Male Subjects

e21556 Background: Prestige Biopharma Pte Ltd is developing HD204, a biosimilar candidate of Bevacizumab (Avastin). Bevacizumab has been approved in the treatment of a variety of metastatic tumours. Bevacizumab, a recombinant humanized monoclonal antibody block angiogenesis which is required for cancer progression by preventing binding of soluble vascular endothelial growth factor (VEGF) to VEGF receptors. Due to heterogeneity nature of antibody therapeutic, the impact on quality of HD204 on safety and pharmacokinetic (PK) was reaffirmed through clinical study to establish clinical similarity between HD204 and Avastin. Methods: Quality attributes identified to influence PK and safety established through comprehensive analytical characterization was used to correlate any potential differences (structural or biological) between the two compounds if any, could result in any clinical meaningful differences in safety and PK in the clinical settings. The PK and safety equivalence of HD204 relative to Avastin was demonstrated in a randomized, single-blind, single-dose, three-arm and parallel-group study clinical Phase I (SAMSON). A total of 120 healthy male subjects randomized 1:1:1 were to receive 1 mg/kg intravenous infusion of either HD204, EU- or US-Avastin. Various PK parameters, safety assessments not limiting to adverse events (AE) and measurement of antidrug antibodies (ADA) and neutralizing antibodies (NAb) were evaluated. Results: The pairwise comparisons of Exposure (AUC0-inf and AUC0-last), maximal concentration (Cmax) established equivalence between the 3 arms. All other PK parameters including half-life, clearance and volume of distribution were comparable between HD204 and Avastin treatment groups. Treatment related TEAEs reported for each group were 25.0%, 30.0% and 25.6% respectively and comparable. There were no treatment-emergent SAEs. Furthermore, none of the subjects treated with HD204 was ADA positive. Conclusions: HD204 demonstrated equivalent PK and safety profile to both US-Avastin and EU-Avastin at 1mg/kg administered as a 90-minute IV infusion to healthy male subjects. A prospective clinical study aimed to demonstrate equivalence in terms of efficacy, PK and safety is currently ongoing. Clinical trial information: 2017-005174-19.

scholarly journals A Mathematical Model For The Special Effects Of Phosphatidylserine On Endocrine Reaction To Reasonable Concentration Exercise In Healthy Male Subjects

2021 ◽  
Vol 12 (3) ◽  
pp. 3555-3559
Author(s):  
A. Leema Rose Et.al
Keyword(s):  
Mathematical Model ◽  
Growth Hormone ◽  
Short Range ◽  
Exponential Model ◽  
Moderate Intensity ◽  
Healthy Male ◽  
Medical Report ◽  
Special Effects ◽  
The Impact ◽  
Male Subjects

In this study, we implement a three-parameter Weibull-G exponential model analysis to analyse the impact of short-range supplementation with a modest dosage of cortisol, lactate, growth hormone and testosterone plasma attentions in healthy male profiles before, during, and after moderate intensity practice.PS supplementation facilitates a desirable athlete state of hormones through stepping up at the rates of cortisol. Ultimately, we conclude that the application part coincides with a mathematical model and the result is linked to the medical report. In the future, this paper will be very beneficial in the medicinal field.

Membrane-type matrix metalloproteinase-9 activity in placental tissue from patients with pre-existing and gestational diabetes mellitus

2000 ◽  
Vol 12 (6) ◽  
pp. 269 ◽  
Author(s):  
Carolina Pustovrh ◽  
Alicia Jawerbaum ◽  
Debora Sinner ◽  
Mario Pesaresi ◽  
Mario Baier ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Matrix Metalloproteinase ◽  
Placental Tissue ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Sod Activity ◽  
No Donors ◽  
Membrane Type ◽  
Metalloproteinase 9

The activity of matrix metalloproteinase (MMP)-9 was evaluated in placental tissue from healthy subjects (controls) and from patients with gestational and pre-existing diabetes mellitus (GDM and PDM, respectively). Compared with controls, MMP-9 activity was greater in placental tissue from patients with PDM and lower in placental tissue from patients with GDM. The modulatory role of nitric oxide (NO) and reactive oxygen species (ROS) on MMP-9 activity in placental tissue was evaluated. In healthy placenta, NO synthase inhibitors diminished MMP-9 activity, whereas NO donors enhanced it. The addition of xanthine/xanthine oxidase or hydrogen peroxide to placental incubates enhanced MMP-9 activity, while the addition of superoxide dismutase (SOD) diminished it. In placental tissue from patients with PDM, MMP-9 activity was stimulated by NO and by ROS. In placental tissue from patients with PDM, concentrations of nitrates/nitrites and thiobarbituric acid-reactive substances (TBARS) were enhanced, whereas SOD activity was decreased, suggesting that elevated concentrations of NO and ROS may be related to the enhanced MMP-9 concentrations found in these tissues. In placenta from GDM patients, in which a diminished concentration of MMP-9 were detected, nitrate/nitrite concentrations were increased, but placental MMP-9 activity did not change in the presence of either NO donors or inhibitors. The activity of MMP-9 in placental tissue from patients with GDM was stimulated by ROS donor systems and was inhibited by the addition of SOD; however, TBARS and SOD concentrations were unchanged in these tissues compared with controls. These findings demonstrate that placental MMP-9 activity is modulated by NO and ROS and that, in diabetic pathology, NO and ROS may determine changes in MMP-9 activity, which are probably involved in the structural and functional abnormalities of diabetic placental tissue.

The effect of acute ammonia exposure on selected immunological parameters of Daphnia similoides (Cladocera)

Crustaceana ◽  
2018 ◽  
Vol 91 (4) ◽  
pp. 403-411
Author(s):  
Youhui Huang ◽  
Ye Liang ◽  
Yiming Li ◽  
Mingqi Cai ◽  
Qichen Jiang ◽  
...  
Keyword(s):  
Ammonia Toxicity ◽  
Whole Body ◽  
Natural Environments ◽  
Toxic Substances ◽  
Sod Activity ◽  
Immunological Parameters ◽  
Immune Parameters ◽  
Significant Change ◽  
High Concentrations ◽  
The Impact

Cladocerans have been widely used as bioassay organisms to evaluate the impact of toxic substances. In this study, the activities of selected immunological enzymes and MDA level were investigated to characterize Daphnia similoides’ immune response to ammonia stress. The activity of ACP and CAT showed no significant change during the test. The activity of AKP was significantly increased in the 0.19 and 0.38 mg/l NH3-N exposure groups after 6 h. After 24 h, a significant depression occurred in the high concentrations. After 24 h, significant decreases in SOD activity were observed at the concentrations of 0.38, 0.57 and 0.76 mg/l NH3-N. After 24 h, a significant change in MDA was also observed in 0.57 and 0.76 mg/l. These results suggest that changes in whole-body immune parameters of Daphnia similoides are effective to predict sub-lethal ammonia toxicity and useful as an early warning tool in monitoring studies of natural environments.

Olive cake reduces glycaemia and lipemia and increases antioxidant enzymes in STZ-induced diabetes in rat erythrocytes and tissues

2019 ◽  
Vol 50 (2) ◽  
pp. 360-372
Author(s):  
Hayet Cherrad ◽  
Sherazede Bouderbala ◽  
Yahiaoui Zidan ◽  
Djamil Krouf
Keyword(s):  
Antioxidant Enzymes ◽  
Heart Muscle ◽  
Design Methodology ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Thiobarbituric Acid Reactive Substances ◽  
Olive Cake ◽  
Sod Activity ◽  
Content Type ◽  
Antioxidant Enzymes Activities

Purpose The purpose of this study is to determine the effect of olive cake (CO) on glycaemia and lipemia and lipid peroxidation and antioxidant enzymes activities in erythrocytes and tissues, in streptozotocin (STZ)-induced diabetic rats. Design/methodology/approach Diabetes was induced by a single intraperitoneal injection of STZ (55 mg/kg BW). In total, 12 diabetic D rats, weighing 260 ± 20 g, were divided into two groups fed a casein diet supplemented (D-OC) or not (D) with OC (7.5 per cent), for four weeks. Findings In D-OC compared with D, glycaemia, total cholesterol and triglycerides values (−40 per cent; p = 0.007, 27 per cent; p = 0.007 and −27 per cent; p = 0.0019). In erythrocyte, liver, kidney, heart, muscle and brain, thiobarbituric acid reactive substances contents were respectively, (−19 per cent; p = 0.03, −32 per cent; p = 0.002, −20 per cent; p = 0.04, −68 per cent; p = 0.003, −74 per cent; p = 0.0003 and −38 per cent; p = 0.04). In erythrocyte, SOD, GSH-Px and CAT activities were respectively, (+14 per cent; p = 0.01, +74 per cent; p = 0.012 and +34 per cent; p = 0.0009). In the liver, kidney, heart and muscle, SOD activity was respectively, (+31 per cent; p = 0.004, +12 per cent; p = 0.038, +43 per cent; p = 0.001 and +23 per cent; p = 0.18). GSH-Px activity was respectively, (+121 per cent; p = 0.0009, 89 per cent; p = 0.0006, + 95 per cent; p = 0.008, +71 per cent; p = 0.02 and +26 per cent; p = 0.01), in the liver, kidney, heart, muscle and brain. Catalase activity was (+21 per cent; p = 0.008) in the liver, (+88 per cent; p = 0.0002 in the kidney, +53 per cent; p = 0,002 in the heart and 83 per cent; p = 0.00004 in the muscle). Originality/value In diabetic rats, OC reduces hyperglycaemia induced by STZ and attenuates triglyceridemia and cholesterolemia. This residue is able to decrease the oxidative stress by increasing the antioxidant enzymes activity in erythrocytes and tissues. The high contents of phytoconstituents present in OC are considered to be responsible for this effect.

Role of antioxidant defenses during estivation and anoxia exposure in the freshwater snail Biomphalaria tenagophila (Orbigny, 1835)

2003 ◽  
Vol 81 (7) ◽  
pp. 1239-1248 ◽  
Author(s):  
Marcus V.R Ferreira ◽  
Antonieta C.R Alencastro ◽  
Marcelo Hermes-Lima
Keyword(s):  
Thiobarbituric Acid ◽  
Freshwater Snail ◽  
Antioxidant Defenses ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Glutathione S Transferase ◽  
Sod Activity ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Gpx Activity

The effects of 24 h of exposure to underwater anoxia and 15 days of estivation (at 26–27°C) on the enzymatic antioxidant system of the hepatopancreas of the freshwater snail Biomphalaria tenagophila (Planorbidae) are described. The effect of 24 h of recovery was also investigated. Catalase activity dropped by 31% during 24 h of anoxia, and superoxide dismutase (SOD) activity was reduced by 43% during the 15 days of estivation. This is consistent with the overall decrease in metabolic rate during estivation or anoxia. Indeed, the heartbeat diminished by 28–36% during estivation (determination was possible for only 4 days) and by 66% after 24 h of anoxia. On the other hand, selenium-dependent glutathione peroxidase (Se-GPX) activity increased during anoxia (from 10 to 14 mU/mg protein) and estivation (by 14%). Glutathione S-transferase (GST) and glutathione reductase activities remained unchanged during estivation and anoxia. Glucose 6-phosphate dehydrogenase activity was unchanged during estivation and recovery. Recovery restored SOD activity. Catalase, Se-GPX, and GST activities during recovery were significantly lower than those of the respective controls. Lipid peroxidation, determined as the level of thiobarbituric acid-reactive substances, was unchanged in the hepatopancreas after 15 days of estivation and 26 h of recovery from estivation. It is possible that the increase in Se-GPX activity during anoxia and estivation, and the maintenance of GST activity, are relevant in minimizing the effects of reactive oxygen species that can be formed upon resumption of aerobic metabolism. Thus, B. tenagophila may have a biochemical strategy of preparation for oxidative stress such as that observed in several other species of anoxia/hypoxia-tolerant animals.

scholarly journals Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freunds Adjuvant (CFA)-Induced Rheumatoid Arthritis in Experimental Model

2021 ◽  
pp. 370-382
Author(s):  
Abdel-Aziz Saeed ◽  
Mohamed El-Shafey ◽  
Gouda K. Helal ◽  
El-Sayed Akool
Keyword(s):  
Rheumatoid Arthritis ◽  
Experimental Model ◽  
Colorimetric Assay ◽  
Mrna Level ◽  
Thiobarbituric Acid ◽  
Concomitant Administration ◽  
Thiobarbituric Acid Reactive Substances ◽  
Sod Activity ◽  
Mda Content ◽  
Inflammatory Effect

Aim: The present work was designed to examine of the potential anti-inflammatory effect of rosuvastatin (ROSV) and/or Lefleunamide (LFLU) against Complete Freunds Adjuvant (CFA)-induced arthritis in rats. Methods: The mRNA level of perxisome proliferator-activated receptor-alpha (PPAR-a) was determined using Real-time PCR. The levels of NF-B, iNOS, IL-6, TNF-a and SOD activity were measured using ELISA. The swollen paws were measured using caliper. The GSH level was measured using colorimetric assay. The level of malondialdehyde (MDA) was determined using thiobarbituric acid reactive substances assay kit. Results: ROSV induced the expression of PPAR-a that suppresses NF-kB as demonstrated by a strong reduction in NF-kB level in animals treated with ROSV. Also, ROSV administration reduced the levels of the inflammatory mediators IL-6 and TNF-a. In addition, iNOS and MDA content as well as expression of MMP-9 and MMP-2 induced by CFA is abrogated in animals treated with ROSV. Also GSH content and SOD activity were highly increased in ROSV-treated animals. Furthermore, the size of right paw induced by CFA was reduced in ROSV-treated rats. Moreover, the histopathological alterations induced by CFA were highly improved in animals treated with ROSV. Similar results were also found in animals treated with LFLU. Importantly, similar effects were obtained in rats treated with both ROSV and LFLU in half doses. Conclusion: This study demonstrates that ROSV as well as LFLU has the ability to inhibit rheumatoid arthritis in experimental model induced by CFA. Importantly, concomitant administration of ROSV and LFLU in half doses synergize against rheumatoid arthritis.

The impact of regular long term physical load on cardiovascular functional parameters in children and adolescent athletes

2013 ◽  
Vol 23 (1) ◽  
pp. 53-59
Author(s):  
Aldona Bartkevičienė ◽  
Dalia Bakšienė ◽  
Pranas Šerpytis
Keyword(s):  
Blood Pressure ◽  
Children And Adolescents ◽  
Cardiovascular System ◽  
The Body ◽  
Physical Load ◽  
Whole Body ◽  
Human Organism ◽  
Control Group ◽  
Functional Parameters ◽  
The Impact

Introduction. Regular physical load determines increase in functional capability of cardiovascular system. On the other hand, the cardiovascular system often appears as a conditional factor, which restricts organism adaptive abilities and limits general organism adaptation to the load. During physical load, not only cardiovascular system is activated, but also complex changes take place in the whole body. Therefore, with the aim to evaluate the functions of various systems, interrelation between them and systemic response of the body to physical load, a complex research on distinctive features of not only functional indices of the cardiovascular system, but also parameters reflecting interrelationship among functional systems of the body and speed of their changes is carried out. The goal of such complex research is the evaluation of adaptive and reserve capabilities of the athlete’s body and individualization and optimization of physical load. The aim of this study was to evaluate the speed of changes of parameters that characterize the functional condition of human organism of children and adolescents athletes and non-athletes controls. Materials and methods. One hundred sixty seven male athletes aged 14.8 (SD1.6, range 12-17 years) participating in basketball, rowing and cycling and168 healthy sedentary controls matched for age, sex and body surface area performed a graded exercise test (Mc. Master) on a cycle ergo-meter. 12 ECG standard derivations were synchronously recorded every second minute. During cycle ergo-meter integrated functional parameters, which could integrally and simple evaluate organism reaction to physical load, were assessed.Results. The present study demonstrates that before reaching the maximum of physical load, the speed of changes JT/RR in athletes and all functional parameters (HR, JT interval, RR interval, systolic blood pressure (SBP), diastolic blood pressure (DBP), pulse blood pressure (SBP-DBP), JT/RR ratio) in the control group decreased and in the last step of physical load has increased. That suggests that during intensive exercise training limits of physiological changes can be exceeded in athletes. Conclusions. Analysis of speed of changes in functional parameters during physical load can be applied for the evaluation of functional state of the human body and the cardiovascular system and aiming to optimize and individualize physical load in athletic children and adolescents.

Chronic hypoxia alters glucose utilization during GSH-dependent detoxication in rat small intestine

1998 ◽  
Vol 274 (2) ◽  
pp. G376-G384 ◽  
Author(s):  
Terry S. Legrand ◽  
Tak Yee Aw
Keyword(s):  
Glucose Utilization ◽  
Lipid Peroxide ◽  
Thiobarbituric Acid ◽  
Sprague Dawley ◽  
Thiobarbituric Acid Reactive Substances ◽  
Disulfide Reduction ◽  
Sprague Dawley Rats ◽  
The Impact

We showed that hypoxia alters glutathione (GSH)-dependent detoxication and induces mucosal metabolic instability. To determine the impact of these changes and the role of reductant supply in intestinal lipid peroxide disposition, pair-fed (16 g/day) Sprague-Dawley rats were exposed to air (20.9% O2; n = 6) or 10% O2( n = 6) for 10 days. Jejunal and ileal everted sacs were exposed to 75 μM peroxidized fish oil with or without 10 mM glucose or 1 mM GSH. Peroxide transport was determined as the abluminal recovery of thiobarbituric acid-reactive substances. Peroxide recovery in hypoxic intestine was twice that in normoxic intestine. Addition of GSH and glucose did not affect peroxide recovery, indicating reduced intracellular GSH-dependent metabolism and enhanced output by the hypoxic intestine. Glucose uptake by normoxic and hypoxic intestine is similar, whereas its utilization for detoxication is decreased in hypoxic cells. Determination of NADPH supply indicates that decreased glucose availability for NADPH production during hypoxia impairs GSH disulfide reduction, compromises hydroperoxide metabolism, and increases peroxide output from hypoxic intestine.

Sign in / Sign up

Export Citation Format

Share Document