scholarly journals Hypolipidemic Activity and Antiatherosclerotic Effect of Polysaccharide ofPolygonatum sibiricumin Rabbit Model and Related Cellular Mechanisms

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Jun-xuan Yang ◽  
Shen Wu ◽  
Xi-liang Huang ◽  
Xiao-quan Hu ◽  
Yi Zhang
Keyword(s):  
Endothelial Cells ◽  
Primary Culture ◽  
Rabbit Model ◽  
Foam Cells ◽  
Hypolipidemic Activity ◽  
Control Group ◽  
Group Model ◽  
Model Control ◽  
Antiatherosclerotic Effect ◽  
Aortic Morphology

Objective. To evaluate the hypolipidemic activity and antiatherosclerotic effect of polysaccharide ofPolygonatum sibiricum(PPGS), which is a kind of Chinese herbal medicine using the rhizome part of the whole herb.Materials and Methods. Thirty rabbits were divided into normal control group, model control group, and PPGS subgroups of 0.8, 1.6, and 3.2 mL/kg/day under random selection. In atherosclerosis model, the effects of PPGS on diverse blood lipids, foam cells number, and aortic morphology were evaluated. In the primary culture of endothelial cells (ECs), the activities of PPGS on both ECs proliferation and ECs injury were studied as well.Results. In atherosclerosis model, the hypolipidemic activities of PPGS were mainly focused on TC, LDL-C, and Lp(a). All changes on these factors were statistically significant compared with model group (P< 0.01), except TG and HDL-C. The intimal foam cell number of PPGS subgroups (0.8, 1.6, and 3.2 mL/kg/day) was significantly reduced than model control (P< 0.01). In the primary culture of endothelial cells (ECs), PPGS showed no effect on cell proliferation but preferred to protect EC from injury and apoptosis induced by H2O2and lipopolysaccharide (LPS).Discussion and Conclusion. The antiatherosclerotic effect of PPGS may be supported by its hypolipidemic activities, improving aortic morphology, and reducing foam cells number and ECs injury.

Experimental Study Oviductus ranae for the Change of α-SMA and TGF-β in Hepatic Fibrosis Rat Liver

2014 ◽  
Vol 912-914 ◽  
pp. 1940-1943
Author(s):  
Yan Li ◽  
Xiao Ou Li ◽  
Feng Hao ◽  
Lei Zhang ◽  
Lei Liu ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Hepatic Fibrosis ◽  
Rat Liver ◽  
Liver Tissue ◽  
Female Rats ◽  
Control Group ◽  
Group Model ◽  
Model Group ◽  
Model Control ◽  
Oviductus Ranae

To evaluate the control effect of Oviductus ranae on liver fibrosis in rats, and the change of TGF-β and α-SMA in liver of. To explore the mechanism of Oviductus ranae decoction on liver fibrosis. Methods Wistar female rats were randomly divided into a blank control group, model control group, colchicines group, Oviductus ranae group. Using the CCl4composite approach to make the rat modle. The course of treat-mart was 12 weeks.After treatment,All the rats was killed,and the materials and blood was taken,and to detect biochemical test of liver function after eight weeks. Investigating the variation of liver histology. Meanwhile detecting protein expression of TGF-β and α-SMA and by immunehistochemical method.Result The general condition of rats in all treatment groups are worse than the blank group,but better than the model group. And the rats in the model group were all occurred in liver fibrosis,and liver fibrosis is the most serious.In a normal rat liver tissue of TGF-β and α-SMA were significantly lower in model group and each treatment group, and there were significant differences, and the TGF-β and α-SMA in expression of liver tissue in model rats of TGF-β and α-SMA the highest. Conclusion: Oviductus ranae can effectively improve liver fibrosis rats induced by CC14liver function.Oviductus ranae can reduce the expression of TGF-β1in liver tissue of hepatic fibrosis rats induced by CCl4in. This may be one of the mechanisms of Oviductus ranae in prevention and treatment of liver fibrosis. Even though both increased expression of TGF-β and α-SMA expression, is able to determine TGF-β and α-SMA for the intervention of liver TGF-β signal transduction pathway in liver fibrosis.

The Effects of Model Similarity and Model Talk on Self-efficacy and Muscular Endurance

1981 ◽  
Vol 3 (1) ◽  
pp. 17-29 ◽  
Author(s):  
Daniel Gould ◽  
Maureen Weiss
Keyword(s):  
Self Efficacy ◽  
Endurance Performance ◽  
Control Group ◽  
Group Model ◽  
Muscular Endurance ◽  
Model Control ◽  
Self Talk ◽  
Efficacy Measures ◽  
Four Levels ◽  
Model Similarity

This study was designed to determine if observing a similar or dissimilar model who makes varying self-efficacy statements influences an observer's efficacy expectations and, in turn, muscular endurance performance. Females (N = 150) were randomly assigned to groups in a 2 × 4 × 3 (model similarity by model talk by trials) factorial design or to a no-model control group. Model similarity was manipulated by having subjects view a female described as a nonathlete (similar) or a male described as a varsity track athlete (dissimilar). The four levels of model talk included: a positive self-talk model who performed and made positive self-efficacy statements, a negative self-talk model who made negative self-efficacy statements, an irrelevant-talk model who made statements unrelated to self-efficacy, and a no-talk model who remained silent throughout the performance. Self-efficacy measures were assessed in addition to performance on three trials of a muscular endurance task. Results revealed that similar model subjects extended their legs significantly longer than dissimilar model and control subjects. Moreover, the similar-positive-talk and similar-no-talk groups performed significantly better than the dissimilar-positive-talk, dissimilar-negative talk, dissimilar-no-talk, and the no-model control groups. Subject self-efficacy, however, was not found to be the major mediating variable affecting these performance changes.

Antioxidant Research of Persimmon Extraction in Ionizing Radiatio Mice

2011 ◽  
Vol 343-344 ◽  
pp. 1198-1206 ◽  
Author(s):  
Xiang Ning Chen ◽  
Wan Shan Hu ◽  
Yuan Hong Xie ◽  
Yu Hua Li ◽  
Lin Bo Guo ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Good Health ◽  
Control Group ◽  
Group Model ◽  
Dosage Group ◽  
Blank Control Group ◽  
Model Control ◽  
Mda Content ◽  
Set Up

Persimmon has good health function. To further investigate the antioxidant function in vivo, we set up a model mice with ionizing radiation. IRC mice were divided randomly into six groups, Blank control group, Model control group, VE control group, Low dosage group (G1), Middle dosage group (G2) and High dosage group (G3) respectively. Orally given persimmon extract for 35 days, mice were treated with γ-rays. Our results indicated that pervious administration of the persimmon extraction obviously increased the liver index of the mice. And pathological inspection by microscope suggested that orally given persimmon extraction obviously protected the liver cells of the mice. Further analysis demonstrated that persimmon extraction decreased the MDA content, and increased the SOD and GSH-PX activity in both the serum and the liver in the mice after the Ionizing radiation. Our results suggested the protection role of persimmon extraction by inhibiting the oxidation reaction in vivo.

scholarly journals The Polysaccharides from Codonopsis pilosula Modulates the Immunity and Intestinal Microbiota of Cyclophosphamide-Treated Immunosuppressed Mice

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1801 ◽  
Author(s):  
Yu-Ping Fu ◽  
Bin Feng ◽  
Zhong-Kai Zhu ◽  
Xin Feng ◽  
Shu-Fan Chen ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Immunoglobulin A ◽  
Control Group ◽  
Group Model ◽  
Codonopsis Pilosula ◽  
Model Control ◽  
Model Control Group ◽  
Ifn Γ ◽  
Immunosuppressed Mice ◽  
Secretory Immunoglobulin

Based on previous studies about microflora regulation and immunity enhancement activities of polysaccharides from Codonopsis pilosula Nannf. var. modesta (Nannf.) L. T. Shen (CPP), there is little study on intestinal mucosal immunity, which is a possible medium for contacting microflora and immunity. In the present study, the BALB/c mice were divided into five groups (eight mice in each group), including a normal group (Con), a model control group (Model), and model groups that were administered CPP (50, 100, 200 mg/kg/d) orally each day for seven days after intraperitoneal injection of 60 mg/kg BW/d cyclophosphamide (CP) for three days. CPP recovered the spleen index and restored the levels of IFN-γ, IL-2, IL-10, as well as serum IgG. In addition, it elevated ileum secretory immunoglobulin A (sIgA), the number of Lactobacillus and acetic acid content in cecum. These results indicated that CPP plays an important role in the protection against immunosuppression, especially mucosa immune damage, and the inhibition of pathogenic bacteria colonization, which could be considered a potential natural source of immunoregulator.

scholarly journals Study on the Effect of Ginsenosides Rb on Blood of Tumor Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Mengmeng Zheng ◽  
Wenxiu Zheng ◽  
Wei Wang ◽  
Hong Guo ◽  
Hui Cao ◽  
...  
Keyword(s):  
Dose Group ◽  
Extraction Process ◽  
Positive Control ◽  
Control Group ◽  
High Dose ◽  
Group Model ◽  
Mice Model ◽  
Distribution Width ◽  
Model Control ◽  
Water Saturated

Objective. The blood of cancer patients is in a state of hypercoagulability, easily leading to thrombosis. Anemia is also a complication of tumors. Anemia and thrombosis affect the treatment of tumor patients. Methods. Ginsenosides Rb were extracted from the stems and leaves of American ginseng using water-saturated ethanol and ethyl acetate in silica gel column. Tumor mice model was established by injecting H22 hepatocellular carcinoma cells into the axilla of mice. Mice were randomly divided into 6 groups: normal control group, model control group, positive control group, low dose group (7 mg/kg), middle dose group (14 mg/kg), and high dose group (35 mg/kg). After 18 days, the blood was obtained by picking the eyeball of mice. The levels of red blood cells (RBC), hemoglobin (HGB), neutrophils/lymphocytes radio (NLR), platelets (PLT), platelet distribution width (PDW), fibrinogen (FIB), and D-Dimer (D-D) were measured and compared in each group of mice. Results. The content of obtained ginsenosides Rb reached 90.05%. This extraction process was simple and reliable. Middle dose of ginsenosides Rb could significantly increase RBC and HGB levels (P<0.05). Moreover, ginsenosides Rb could significantly reduce NLR, PLT, PDW, FIB, and D-D (P<0.01). Conclusion. ginsenosides Rb could significantly improve anaemia and hypercoagulation of blood in cancer mice. Ginsenosides Rb are a potential anticoagulant and antianemia drug in treating cancer.

Effect of triamcinolone acetonide-loaded nanoparticles on diabetic retinopathy in rats

2020 ◽  
Vol 10 (9) ◽  
pp. 1511-1517
Author(s):  
Chengyan Li ◽  
Guihong Zhao ◽  
Lianwei Lu ◽  
Xiuping Du
Keyword(s):  
Diabetic Retinopathy ◽  
Blood Glucose ◽  
Triamcinolone Acetonide ◽  
Glycosylated Hemoglobin ◽  
Control Group ◽  
Group Model ◽  
Pathological Changes ◽  
Model Control ◽  
Model Control Group

Triamcinolone acetonide acetate (TAA)-loaded nanostructured lipid carriers (NLC) were prepared to investigate their transdermal absorption in vitro and establish a diabetic retinopathy (DR) rat model. To evaluate the effect of triamcinolone acetonide-loaded nanoparticle capsules on improving the structure of the retina and pancreas, we measured blood glucose levels and investigated the pathological changes in the retina and pancreas. The effect of triamcinolone acetonide-loaded nanoparticle capsules on the morphology of the retina and pancreas of the rats with DR was determined and compared among the normal group, model control group, positive control group, and triamcinolone acetonide-loaded nanoparticle group. After treatment with triamcinolone acetonideloaded nanoparticle capsules for 2 months, the blood glucose and glycosylated hemoglobin levels were significantly lower (P < 0.01) and the pathological changes were less severe in the triamcinolone acetonide-loaded nanoparticle group than in the model control group. In addition, the arrangement of the photoreceptor cell layers in the retina was organized, intracellular and extracellular edema in each layer was reduced compared with that in the model control group, the capillary lumen was not occluded, and the peripheral cells were slightly edematous in the triamcinolone acetonide-loaded nanoparticle group. Triamcinolone acetonide-loaded nanoparticle capsules could effectively reduce the blood glucose and glycosylated hemoglobin levels and improve the structure of the retina and pancreas in the rats with DR.

scholarly journals Guanxinkang Decoction Exerts Its Antiatherosclerotic Effect Partly through Inhibiting the Endoplasmic Reticulum Stress

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Hao Wang ◽  
Zhi-Min Zheng ◽  
Bu-Lang Gao
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Protein Expression ◽  
Dose Group ◽  
Control Group ◽  
Blank Control Group ◽  
Apoptosis Rate ◽  
Model Control ◽  
Model Control Group ◽  
Antiatherosclerotic Effect

Purpose. To investigate the antiatherosclerotic effect of Guanxinkang (GXK) decoction on the apoptosis, mitochondrial membrane potential (MMP), and endoplasmic reticulum stress (ERS) of human umbilical vein endothelial cells (HUVEC) pretreated with homocysteinemia (HCY).Materials and Methods. HUVEC were randomly divided into 5 groups: (1) blank control group (control), (2) model control group (model), (3) GXK low dose group, (4) GXK medium dose group, and (5) GXK high dose group. For the three GXK groups, HCY was given to reach the concentration of 3.0 mmol/L after HUVEC had been incubated with rabbit serum containing GXK for two hours. At 3, 6, 12, and 24 h after HCY had been incubated with the cells, the HUVEC were collected for test of the apoptosis rate, MMP, and GRP78 protein (reflecting ERS).Results. In the model control group, the apoptosis rate and GRP 78 protein expression of HUVEC significantly increased (P<0.05), while MMP significantly decreased (P<0.05) compared with the blank control group. After GXK treatment of medium and high doses, the apoptosis rate and the GRP 78 protein expression significantly (P<0.05) decreased, while MMP significantly increased (P<0.05) in a time-dependent manner compared with the model control group.Conclusion. GXK can antagonize the injury of HUVEC caused by HCY and the antagonism effect increases with the concentration and treatment duration of GXK, with the possible mechanism of GXK antagonism being through inhibiting ERS caused by HCY.

scholarly journals Effects of Acupuncture Applied to Sanyinjiao with Different Stimuli on Uterine Contraction and Microcirculation in Rats with Dysmenorrhea of Cold Coagulation Syndrome

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Wing-sze Hsu ◽  
Xiao-yu Shen ◽  
Jia-min Yang ◽  
Li Luo ◽  
Ling Zhang ◽  
...  
Keyword(s):  
Uterine Contraction ◽  
Female Rats ◽  
Saline Control ◽  
Control Group ◽  
Group Model ◽  
Stimulus Group ◽  
Deep Insertion ◽  
Model Control ◽  
De Qi ◽  
Cold Coagulation

In this study, we try to evaluate the effects of acupuncture stimulation with different amounts at Sanyinjiao (SP6) on uterine contraction and uterus microcirculation in rats with dysmenorrhea of cold coagulation syndrome and to explore whether there is direct relativity between “De qi” and needle stimulus intensity. Diestrus female rats were randomly divided into 4 groups, including saline control group, model control group, “A” stimulus group (with strong stimulus), and “B” stimulus group (with weak stimulus). We found that dysmenorrhea rats of the cold coagulation syndrome present a high intensity in uterine tension and high contraction of microvascular diameter. Acupuncture applied with two different stimuli could relieve the symptoms, but, compared with “B” stimulus, “A” stimulus leads to better outcomes on reducing uterine contraction and increasing diameter of uterine microvascular; moreover, hand manipulation during needling mediates the curative effect on the microvascular diameter. Our finding indicates that using thick needles and deep insertion with hand manipulation are more effective and achieve desired level of “De qi” in dysmenorrhea rats.

scholarly journals Protection ofSalvia miltiorrhizaeto the Spleen and Thymus of Rats with Severe Acute Pancreatitis or Obstructive Jaundice

2009 ◽  
Vol 2009 ◽  
pp. 1-14 ◽  
Author(s):  
Zhang Xiping ◽  
Li Chuyang ◽  
Zhang Jie ◽  
Ruan Yuefang ◽  
Ma Meili
Keyword(s):  
Staining Intensity ◽  
Treated Group ◽  
Control Group ◽  
Therapeutic Effects ◽  
Group Model ◽  
Bax Protein ◽  
Severity Scores ◽  
Model Control ◽  
Model Control Group ◽  
Salvia Miltiorrhizae

Objective. To investigate the therapeutic effects and mechanism ofSalvia miltiorrhizaein the treatment of SAP and OJ.Methods. A total of 288 rats were used for SAP- and OJ-associated experiments. The rats were randomly divided into sham-operated group, model control group and treated group. The mortality rates of rats, contents of endotoxin andPLA2in blood, patholodgical changes of different indexes in spleen and thymus were observed.Results. The contents of endotoxin and PLA2 in treated group were significantly lower than those in model control group.The pathological severity scores of spleen and thymus of SAP rats as well as that of spleen of OJ rats in treated groups were significantly lower than those in model control groups (P<.05). The staining intensity as well as the product of the staining intensity and positive rate of Bax protein of spleen in model control group were significantly higher than those in treated groups (P<.01) , and the apoptosis index of spleen in treated group was significantly lower than that in model control group (P<.01).Conclusion.Salvia miltiorrhizaeexerts protective effects on the spleen and thymus of SAP rats and spleen of OJ rats.

scholarly journals Protective Effects ofSalvia miltiorrhizaeon Multiple Organs of Rats with Obstructive Jaundice

2009 ◽  
Vol 2009 ◽  
pp. 1-9 ◽  
Author(s):  
Zhang Ling ◽  
Zhang Xiping ◽  
Qiu Fengmei ◽  
Yan Ping ◽  
Cheng Qihui
Keyword(s):  
Obstructive Jaundice ◽  
Treated Group ◽  
Control Group ◽  
Group Model ◽  
Protective Effects ◽  
Multiple Organs ◽  
Model Control ◽  
Model Control Group ◽  
Salvia Miltiorrhizae ◽  
The Impact

Purpose. we aim to explore the protective effects ofSalvia miltiorrhizaeinjection on multiple organs of obstructive jaundice (OJ) rats through observing the impact of this injection on the pathological alterations in these organs and the contents of endotoxin,PLA2, and TNF-αin the blood.Methods. A total of 90 mice were randomly divided into sham-operated group, model-control group, andSalvia miltiorrhizae-treated group (n=30). According to the duration of postoperative administration, each group was further divided into two subgroups, namely, 21 d subgroup (consecutive administration for 21 d,n=15) and 28 d subgroup (consecutive administration for 28 d,n=15). After administration, the pathological alterations in multiple organs were observed and the contents of endotoxin,PLA2, and TNF-αin the blood were determined.Results. Compared to model control group, the number of dead rats in treated group decreased though there was no statistical difference between the two groups. The pathological alterations in the liver, kidney, and spleen in treated group showed varying degrees of mitigation. At all time points, the contents of plasma endotoxin declined significantly. On day 28, plasmaPLA2content in treated group was significantly lower than that in model-control group.Conclusion.Salvia miltiorrhizaeinjection is able to obviously reduce the contents of inflammatory mediators in the blood of OJ rats and exert some protective effects on multiple organs of these rats.

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