scholarly journals Identification of Proteins Implicated in the Increased Heart Rate in ShenSongYangXin-Treated Bradycardia Rabbits by iTRAQ-Based Quantitative Proteomics

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Zhouying Liu ◽  
Jian Huang ◽  
Youping Huo ◽  
Jing Gong ◽  
Yinhui Zhang ◽  
...  
Keyword(s):  
Heart Rate ◽  
Quantitative Proteomics ◽  
Sinoatrial Node ◽  
Absolute Quantitation ◽  
Treatment Groups ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Isobaric Tags ◽  
Altered Proteins ◽  
Chinese Medicine Treatment

The present study tries to identify proteins implicated in bradycardia rabbits in hearts after ShenSongYangXin (SSYX, a traditional Chinese medicine) treatment. Eighteen adult rabbits were randomly assigned to three groups: sham, model, and SSYX treatment groups. Heart rate was recorded in rabbits and proteins were isolated from ventricular muscle. We used isobaric tags for elative and absolute quantitation (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry to identify altered proteins after SSYX treatment. The heart rate decreased after six weeks due to the injury of the sinoatrial node in the model group. This effect was partially reversed by 4-week SSYX treatment. A total of a2988 proteins were quantified by performing the iTRAQ-based experiments. Of these, 86 proteins were differentially expressed according to our criteria (42 upregulated and 44 downregulated). The identification of key proteins implicated in the treatment of bradycardia could serve as a foundation to better understand and further explore the molecular mechanism of SSYX treatment.

scholarly journals Quantitative proteomic profiling of Cervicovaginal fluid from pregnant women with term and preterm birth

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Young Eun Kim ◽  
Kwonseong Kim ◽  
Han Bin Oh ◽  
Sung Ki Lee ◽  
Dukjin Kang
Keyword(s):  
Preterm Birth ◽  
Pregnant Women ◽  
Neonatal Morbidity ◽  
Differentially Expressed ◽  
Proteomic Profiling ◽  
Absolute Quantitation ◽  
Term Birth ◽  
Liquid Chromatography Tandem Mass ◽  
Isobaric Tags ◽  
Cervicovaginal Fluid

Abstract Background Preterm birth (PTB) is one of major causes of perinatal mortality and neonatal morbidity, but knowledge of its complex etiology is still limited. Here we present cervicovaginal fluid (CVF) protein profiles of pregnant women who subsequently delivered at spontaneous preterm or term, aiming to identify differentially expressed CVF proteins in PTB and term birth. Methods The CVF proteome of women who sequentially delivered at preterm and term was analyzed using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with two-dimensional nanoflow liquid chromatography-tandem mass spectrometry (2D-nLC-MS/MS). We compared the CVF proteome of PTB (n = 5) and control subjects (term birth, n = 7) using pooled control CVF (term birth, n = 20) as spike-in standard. Results We identified 1294 CVF proteins, of which 605 were newly identified proteins. Of 990 proteins quantified in both PTB and term birth, 52 proteins were significantly up/down-regulated in PTB compared to term birth. The differentially expressed proteins were functionally associated to immune response, endopeptidase inhibitors and structural constituent of cytoskeleton. Finally, we confirm the down-regulation of SERPINB7 (a serine-type protease inhibitor) in PTB compared to control by Western blot. Conclusions Taken together, our study provide quantitative CVF proteome profiles of pregnant women who ultimately delivered at preterm and term. These promising results could help to improve the understanding of PTB etiology and to discover biomarkers for asymptomatic PTB.

scholarly journals Differentiating Two Closely Related Alexandrium Species Using Comparative Quantitative Proteomics

Toxins ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 7
Author(s):  
Bryan John J. Subong ◽  
Arturo O. Lluisma ◽  
Rhodora V. Azanza ◽  
Lilibeth A. Salvador-Reyes
Keyword(s):  
Quantitative Proteomics ◽  
Polyketide Synthase ◽  
Harmful Algal Bloom ◽  
Feedback Mechanism ◽  
Type I ◽  
Absolute Quantitation ◽  
Alexandrium Minutum ◽  
2D Dige ◽  
Negative Feedback Mechanism ◽  
Isobaric Tags

Alexandrium minutum and Alexandrium tamutum are two closely related harmful algal bloom (HAB)-causing species with different toxicity. Using isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics and two-dimensional differential gel electrophoresis (2D-DIGE), a comprehensive characterization of the proteomes of A. minutum and A. tamutum was performed to identify the cellular and molecular underpinnings for the dissimilarity between these two species. A total of 1436 proteins and 420 protein spots were identified using iTRAQ-based proteomics and 2D-DIGE, respectively. Both methods revealed little difference (10–12%) between the proteomes of A. minutum and A. tamutum, highlighting that these organisms follow similar cellular and biological processes at the exponential stage. Toxin biosynthetic enzymes were present in both organisms. However, the gonyautoxin-producing A. minutum showed higher levels of osmotic growth proteins, Zn-dependent alcohol dehydrogenase and type-I polyketide synthase compared to the non-toxic A. tamutum. Further, A. tamutum had increased S-adenosylmethionine transferase that may potentially have a negative feedback mechanism to toxin biosynthesis. The complementary proteomics approach provided insights into the biochemistry of these two closely related HAB-causing organisms. The identified proteins are potential biomarkers for organismal toxicity and could be explored for environmental monitoring.

scholarly journals Contribution of N-Glucuronidation to Efavirenz EliminationIn Vivoin the Basal and Rifampin-Induced Metabolism of Efavirenz

2011 ◽  
Vol 55 (4) ◽  
pp. 1504-1509 ◽  
Author(s):  
Doo-Yeoun Cho ◽  
Evan T. Ogburn ◽  
David Jones ◽  
Zeruesenay Desta
Keyword(s):  
Enzymatic Hydrolysis ◽  
Crossover Trial ◽  
Plasma Concentrations ◽  
Treated Group ◽  
Pharmacokinetic Parameters ◽  
Treatment Groups ◽  
Chromatography Tandem Mass Spectrometry ◽  
Significant Difference ◽  
Liquid Chromatography Tandem Mass

ABSTRACTIn this study, the contribution of efavirenz N-glucuronidation to efavirenz eliminationin vivowas assessed. In a two-period placebo-controlled crossover trial design, a single 600-mg oral dose of efavirenz was administered to healthy volunteers (n= 10) pretreated with placebo pills or 600 mg/day rifampin orally for 10 days. Urine and plasma concentrations of efavirenz and 8-hydroxyefavirenz were measured by the liquid chromatography-tandem mass spectrometry method after enzymatic hydrolysis with β-glucuronidase (conjugated and unconjugated) and without enzymatic hydrolysis (unconjugated). Pharmacokinetic parameters of efavirenz within the placebo- or rifampin-treated group obtained after enzymatic hydrolysis did not show any statistically significant difference compared with those obtained without enzymatic hydrolysis (P> 0.05; pairedttest, two-tailed). The amount of efavirenz excreted over 12 h was significantly larger after enzymatic hydrolysis in both the placebo (P= 0.007) and rifampin (P= 0.0001) treatment groups, supporting the occurrence of direct N-glucuronidation of efavirenz, but the relevance of this finding is limited because the amount of efavirenz excreted as unchanged or conjugated in urine is less than 1% of the dose administered. In both the placebo- and rifampin-treated groups, plasma concentrations of 8-hydroxyefavirenz and the amount excreted over 12 h were significantly larger (P< 0.00001) after enzymatic hydrolysis than without enzymatic hydrolysis. These findings suggest that although the occurrence of direct efavirenz N-glucuronidation is supported by the urine data, the abundance of efavirenzN-glucuronide in plasma is negligible and that the contribution of the N-glucuronidation pathway to the overall clearance of efavirenz seems minimal.

scholarly journals Quantitative Proteomics Analysis by Isobaric Tags for Relative and Absolute Quantitation Identified Lumican as a Potential Marker for Acute Aortic Dissection

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Guorong Gu ◽  
Weizhong Cheng ◽  
Chenling Yao ◽  
Jun Yin ◽  
Chaoyang Tong ◽  
...  
Keyword(s):  
Aortic Dissection ◽  
Quantitative Proteomics ◽  
Acute Aortic Dissection ◽  
Serum Marker ◽  
Serum Biomarkers ◽  
Serum Samples ◽  
Absolute Quantitation ◽  
Itraq Analysis ◽  
Potential Marker ◽  
Isobaric Tags

Acute aortic dissection (AAD) is a serious vascular disease. Currently the diagnosis relies on clinical and radiological means whereas serum biomarkers are lacking. The purpose of this study was to identify potential serum biomarkers for AAD using isobaric tags for relative and absolute quantitation (iTRAQ) approach. A total of 120 serum samples were collected from three groups: AAD patients (n=60), patients with acute myocardial infarction (AMI,n=30), and healthy volunteers (n=30), whereas the first 10 samples from each group were used for iTRAQ analysis. Using iTRAQ approach, a total of 174 proteins were identified as significantly different between AAD patients and healthy subjects. Among them, forty-six proteins increased more than twofold, full-scale analysis using serum sample for the entire 120 subjects demonstrated that Lumican level was significantly increased relative to control and AMI samples. Further, Lumican level correlated with time from onset to admission in AAD but not AMI samples. Using iTRAQ approach, our study showed that Lumican may be a potential AAD-related serum marker that may assist the diagnosis of AAD.

Sign in / Sign up

Export Citation Format

Share Document