scholarly journals Polymorphonuclear Leukocyte Apoptosis Is Accelerated by Sulfatides or Sulfatides-TreatedSalmonellaTyphimurium Bacteria

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Zoryana V. Grishina ◽  
Galina M. Viryasova ◽  
Yulia M. Romanova ◽  
Galina F. Sud’ina
Keyword(s):  
Flow Cytometry ◽  
Polymorphonuclear Leukocyte ◽  
Human Neutrophil ◽  
Apoptotic Index ◽  
Neutrophil Apoptosis ◽  
Apoptotic Cells ◽  
Galactosidase Activity ◽  
Leukocyte Apoptosis ◽  
Intracellular Compartments ◽  
Ceramide Content

Neutrophils die by apoptosis following activation and uptake of microbes or enter apoptosis spontaneously at the end of their lifespan if they do not encounter a pathogen. Here we report that sulfatides or sulfatides-treatedSalmonellaTyphimurium bacteria accelerated human neutrophil apoptosis. Neutrophil apoptosis was examined by flow cytometry. Sulfatides caused prominent increase in percentage of apoptotic cells after 2.5 hrs of incubation.SalmonellaTyphimurium bacteria by themselves did not affect the basal level of apoptosis in neutrophil population. When neutrophils were added toS.Typhimurium “opsonized” by sulfatides, apoptotic index significantly increased, whereas the number of phagocyting cells was not influenced. Sulfatides’ proapoptotic effect was strongly dependent on the activity ofβ-galactosidase; inhibition of this enzyme impaired its potency to accelerate apoptosis. These data support the mechanism of neutrophil apoptosis triggering based on sulfatides’ ability to accumulate in intracellular compartments and mediate successive increase in ceramide content resulting fromβ-galactosidase activity.

Modulation of Polymorphonuclear Leukocyte Apoptosis in the Critically Ill by Removal of Cytokines with Continuous Hemodiafiltration

2004 ◽  
Vol 22 (2) ◽  
pp. 188-197 ◽  
Author(s):  
Takeshi Hirano ◽  
Hiroyuki Hirasawa ◽  
Shigeto Oda ◽  
Hidetoshi Shiga ◽  
Kazuya Nakanishi ◽  
...  
Keyword(s):  
Critically Ill ◽  
Polymorphonuclear Leukocyte ◽  
Continuous Hemodiafiltration ◽  
Leukocyte Apoptosis

scholarly journals Hsp27 Regulates Akt Activation and Polymorphonuclear Leukocyte Apoptosis by Scaffolding MK2 to Akt Signal Complex

2007 ◽  
Vol 282 (30) ◽  
pp. 21598-21608 ◽  
Author(s):  
Rui Wu ◽  
Hina Kausar ◽  
Paul Johnson ◽  
Diego E. Montoya-Durango ◽  
Michael Merchant ◽  
...  
Keyword(s):  
Polymorphonuclear Leukocyte ◽  
Akt Activation ◽  
Leukocyte Apoptosis

scholarly journals Human Neutrophil Calprotectin Reduces the Susceptibility of Borrelia burgdorferi to Penicillin

2006 ◽  
Vol 74 (4) ◽  
pp. 2468-2472 ◽  
Author(s):  
Ruth R. Montgomery ◽  
Kimberly Schreck ◽  
Xiaomei Wang ◽  
Stephen E. Malawista
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Polymorphonuclear Leukocyte ◽  
Human Neutrophil ◽  
Penicillin G ◽  
Cytoplasmic Protein ◽  
Bacterial Division ◽  
Therapeutic Doses

ABSTRACT Borrelia burgdorferi, the spirochetal agent of Lyme disease, is susceptible to killing by a variety of polymorphonuclear leukocyte (PMN) components. Some are most effective against metabolically active B. burgdorferi. The abundant PMN cytoplasmic protein calprotectin, elevated 10- to 100-fold in inflammation, inhibits the growth of spirochetes through chelation of the essential cation, Zn. Since the action of some therapeutic antibiotics depends on bacterial division, we investigated the antibiotic sensitivities of spirochetes in calprotectin. In physiologic calprotectin, B. burgdorferi is not eliminated by therapeutic doses of penicillin G; in contrast, doxycycline is effective. Calprotectin may modify the clearance of spirochetes at sites of inflammation.

Autoregulation mechanism of human neutrophil apoptosis during bacterial infection☆

2008 ◽  
Vol 45 (7) ◽  
pp. 2087-2096 ◽  
Author(s):  
Marcos G. Ocaña ◽  
Víctor Asensi ◽  
Ángel H. Montes ◽  
Álvaro Meana ◽  
Antonio Celada ◽  
...  
Keyword(s):  
Bacterial Infection ◽  
Human Neutrophil ◽  
Neutrophil Apoptosis
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