scholarly journals Synthesis and Characterization of Chitosan-Saponin Nanoparticle for Application in Plasmid DNA Delivery

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Faruku Bande ◽  
Siti Suri Arshad ◽  
Mohd Hair Bejo ◽  
Shafiu Abdullahi Kamba ◽  
Abdul Rahman Omar
Keyword(s):  
Electron Microscopy ◽  
Delivery System ◽  
Plasmid Dna ◽  
Enzymatic Degradation ◽  
Dna Delivery ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Rapid Decomposition ◽  
Before And After ◽  
Low Cytotoxicity

Nonviral delivery system receives attention over the last decade. Chitosan (CS) is a cationic polymer whereas saponin (SP) is classified as glycoside. In this study, a spherically-shaped CS-SP nanoparticle was synthesized and characterized. The ability of the nanoparticle to protect DNA from enzymatic degradation, its thermostability and cytotoxicity were evaluated. The particle size was found below 100 nm as determined by Zetasizer, transmission electron microscopy (TEM), and field scanning electron microscopy (FSEM) results. The surface charge ranges from 43.7 mV to 38.5 mV before and after encapsulation with DNA plasmid, respectively. In terms of thermostability, Thermal Gravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) revealed that CS-SP nanoparticle had a melting temperature of 110°C, with rapid decomposition occurring at 120°C. Encapsulation of DNA with the synthesized nanoparticle was evidenced by changes in the FTIR spectra including characteristic peaks at 3267.39 and 1635.58 cm−1, wavenumbers. Additional peak was also observed at 1169.7 cm−1following encapsulation. Electrophoretic mobility showed that CS-SP nanoparticle protected plasmid DNA from enzymatic degradation, while cell viability assays confirmed that the synthesized nanoparticle exhibited low cytotoxicity at different concentrations in avian cells. Taken together these, CS-SP nanoparticle showed potentials for applications as a DNA delivery system.

Construction of Dendritic-Linear Brushes on Silica Nanoparticles by Electrostatic Assembly

2013 ◽  
Vol 652-654 ◽  
pp. 490-494
Author(s):  
Bo Shi ◽  
Liang Liang ◽  
Bo Lin ◽  
Min Hua Qi
Keyword(s):  
Thermo Gravimetric Analysis ◽  
Gravimetric Analysis ◽  
Linear Segment ◽  
Benzyl Ether ◽  
Scanning Calorimetry ◽  
Thermo Gravimetric ◽  
H Nmr ◽  
Electrostatic Assembly ◽  
Before And After ◽  
Assembly Behavior

Using dendritic bromide poly (benzyl ether), G2-Br, as macroinitiator, dendritic-linear polymer (G2-PS-NHSO3Na) was obtained via atom transfer radical polymerization and nucleophilic substitution of G2-PS-Br. G2-PS-NHSO3Na was constructed on cationic silica by both electrostatic attraction and steric effort coming from dendritic block of G2-PS-NHSO3Na. 1H-NMR and differential scanning calorimetry were used to characterize the structure and thermal behavior of G2-PS-NHSO3Na; thermo-gravimetric analysis and TEM were applied to analysis the assembly amount of G2-PS-NHSO3Na and the dispersibility of silica before and after assembly. The results show that G2-PS-NHSO3Na can be synthesized successfully and has exact structure; it can be constructed on cationic silica with 27% assembly amount; the aggregation of silica can be prevented effectively by the dendritic-linear brushes, but the molecular weight of linear segment and the generation of dendritic block will influence the assembly behavior and the amount of the dendritic-linear brushes.

scholarly journals SiO2 Coated Up-conversion Nanomaterial Doped with Ag Nanoparticles for Micro-CT Imaging

2021 ◽  
Author(s):  
Wei Zhang ◽  
Yanli Lu ◽  
YANG ZANG ◽  
Jinhui Han ◽  
Qingyun Xiong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Luminescence Intensity ◽  
Ag Nanoparticles ◽  
Fluorescence Spectra ◽  
Cell Counting ◽  
Micro Ct ◽  
Transmission Electron ◽  
Before And After ◽  
Sio2 Nanocomposites ◽  
Low Cytotoxicity

In this study, a new method for synthesizing Ag-NaYF4:Yb3+/Er3+ @ SiO2 nanocomposites was introduced. Using a hydrothermal method, the synthesized Yb3+- and Er3+-codoped NaYF4 upconversion luminescent materials and Ag nanoparticles were doped into upconversion nanomaterials and coated with SiO2 up-conversion nanomaterials. This material is known as Ag-UCNPs-SiO2’ it improves both the luminous intensity because of the doped Ag nanoparticles and has low cytotoxicity because of the SiO2 coating. The morphology of UCNPs was observed using scanning electron microscopy (SEM), and the mapping confirmed the successful doping of Ag nanoparticles. Successful coating of SiO2 was confirmed using transmission electron microscopy (TEM). Fluorescence spectra were used to compare changes in luminescence intensity before and after doping Ag nanoparticles. The reason for the increase in luminescence intensity after doping with Ag nanoparticles was simulated using first-principles calculations. The cytotoxicity of Ag-UCNPs-SiO2 was tested via the cell counting kit-8 (CCK-8) method, and its imaging ability was characterized using the micro-CT method.

scholarly journals SiO2 Coated Up-Conversion Nanomaterial Doped with Ag Nanoparticles for Micro-CT Imaging

Nanomaterials ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3395
Author(s):  
Wei Zhang ◽  
Yanli Lu ◽  
Yang Zang ◽  
Jinhui Han ◽  
Qingyun Xiong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Luminescence Intensity ◽  
Ag Nanoparticles ◽  
Fluorescence Spectra ◽  
Cell Counting ◽  
Micro Ct ◽  
Transmission Electron ◽  
Before And After ◽  
Sio2 Nanocomposites ◽  
Low Cytotoxicity

In this study, a new method for synthesizing Ag-NaYF4:Yb3+/Er3+ @ SiO2 nanocomposites was introduced. Using a hydrothermal method, the synthesized Yb3+- and Er3+-codoped NaYF4 up-conversion luminescent materials and Ag nanoparticles were doped into up-conversion nanomaterials and coated with SiO2 up-conversion nanomaterials. This material is known as Ag-UCNPs@SiO2, it improves both the luminous intensity because of the doped Ag nanoparticles and has low cytotoxicity because of the SiO2 coating. The morphology of UCNPs was observed using scanning electron microscopy (SEM), and the mapping confirmed the successful doping of Ag nanoparticles. Successful coating of SiO2 was confirmed using transmission electron microscopy (TEM). Fluorescence spectra were used to compare changes in luminescence intensity before and after doping Ag nanoparticles. The reason for the increase in luminescence intensity after doping with Ag nanoparticles was simulated using first-principles calculations. The cytotoxicity of Ag-UCNPs@SiO2 was tested via the cell counting kit-8 (CCK-8) method, and its imaging ability was characterized using the micro-CT method.

scholarly journals Структура и свойства пленок SiO-=SUB=-x-=/SUB=-, полученных химическим травлением лент аморфного сплава

2018 ◽  
Vol 60 (4) ◽  
pp. 701
Author(s):  
В.А. Федоров ◽  
А.Д. Березнер ◽  
А.И. Бескровный ◽  
Т.Н. Фурсова ◽  
А.В. Павликов ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Chemical Etching ◽  
Amorphous Ribbon ◽  
Amorphous Structure ◽  
Chemical Compositions ◽  
Scanning Calorimetry ◽  
Transparent Films ◽  
Before And After ◽  
Iron Based ◽  
Raman And Ir Spectroscopy

AbstractThe structure and the physical properties of amorphous SiO_ x films prepared by chemical etching of an iron-based amorphous ribbon alloy have been studied. The neutron diffraction and also the atomicforce and electron microscopy show that the prepared visually transparent films have amorphous structure, exhibit dielectric properties, and their morphology is similar to that of opals. The samples have been studied by differential scanning calorimetry, Raman and IR spectroscopy before and after their heat treatment. It is found that annealing of the films in air at a temperature of 1273 K leads to a change in their chemical compositions: an amorphous SiO_2 compound with inclusions of SiO_2 nanocrystals (crystobalite) forms.

scholarly journals Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity

2013 ◽  
pp. 1361 ◽  
Author(s):  
Shinji Takeoka ◽  
Sarker ◽  
Aoshima ◽  
Hokama ◽  
Inoue ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery ◽  
Cationic Liposomes ◽  
Low Cytotoxicity

Structure and biological compatibility of polycaprolactone/zinc-hydroxyapatite electrospun nanofibers for tissue regeneration

2021 ◽  
pp. 088391152110224
Author(s):  
Maria Clara Guimaraes Pedrosa ◽  
Susana Azevedo dos Anjos ◽  
Elena Mavropoulos ◽  
Pablo Leite Bernardo ◽  
José Mauro Granjeiro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Physicochemical Properties ◽  
Tissue Regeneration ◽  
Electrospun Nanofibers ◽  
Gravimetric Analysis ◽  
Electrospun Membrane ◽  
Scanning Calorimetry ◽  
X Ray ◽  
Biological Compatibility ◽  
Irregular Cell

Although guided tissue regeneration (GTR) is a useful tool for regenerating lost tissue as bone and periodontal tissue, a biocompatible membrane capable of regenerating large defects has yet to be discovered. This study aimed to characterize the physicochemical properties and biological compatibility of polycaprolactone (PCL) membranes associated with or without nanostructured hydroxyapatite (HA) (PCL/HA) and Zn-doped HA (PCL/ZnHA), produced by electrospinning. PCL, PCL/HA, and PCL/ZnHA were characterized by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and differential scanning calorimetry (DSC). Nanoparticles of HA or ZnHA were homogeneously distributed and dispersed inside the PCL fibers, which decreased the fiber thickness. At 1 wt% of HA or ZnHA, these nanoparticles acted as nucleating agents. Moreover, HA and ZnHA increased the onset of the degradation temperature and thermal stability of the electrospun membrane. All tested membranes showed no cytotoxicity and allowed murine pre-osteoblast adhesion and spreading; however, higher concentrations of PCL/ZnHA showed less cells and an irregular cell morphology compared to PCL and PCL/HA. This article presents a cytocompatible, electrospun, nanocomposite membrane with a novel morphology and physicochemical properties that make it eligible as a scaffold for GTR.

scholarly journals Lysozyme-Induced Degradation of Chitosan: The Characterisation of Degraded Chitosan Scaffolds

2017 ◽  
Vol 1 (1) ◽  
pp. 12-22 ◽  
Author(s):  
Andrea Lončarević ◽  
Marica Ivanković ◽  
Anamarija Rogina
Keyword(s):  
Drug Delivery ◽  
Weight Loss ◽  
Enzymatic Degradation ◽  
Gravimetric Analysis ◽  
Degradation Behaviour ◽  
Porous Chitosan ◽  
Before And After ◽  
Glycoside Bond ◽  
Chitosan Scaffolds

Up till now, chitosan has confirmed its versatile application in skin, cartilage and bone tissue engineering, as well as in drug delivery applications. This study is focused on enzymatic degradation of porous chitosan structures usually designed for mentioned purposes. In vitro degradation was monitored during four weeks of incubation at physiological temperature and in two different media, phosphate buffer saline solution and water. The scaffolds were characterised before and after enzymatic degradation using scanning electron microscopy and infrared spectroscopy with Fourier transformations (FTIR). According to the gravimetric analysis, higher weight loss of chitosan scaffolds was observed in buffered medium with respect to the water. The results implied that the total weight loss obtained in buffer involves physical dissolution of chitosan and lysozyme cleavage of glycoside bond. Importantly, FTIR identification of chitosan scaffolds after enzymatic degradation indicated the absence of lysozyme activity in water, indicating that weight loss is a result of the chitosan dissolution. This finding greatly impacts design of degradation experiments and characterisation of degradation behaviour of chitosan-based materials utilised as implants or drug delivery systems.

Investigating the Biodegradability and Physical Properties of Starch Derived Bioplastic Films Reinforced with Nanosilica

2018 ◽  
Vol 18 (06) ◽  
pp. 1850037 ◽  
Author(s):  
Anagha Ashok ◽  
C. R. Rejeesh
Keyword(s):  
Electron Microscopy ◽  
Thermal Stability ◽  
Thermo Gravimetric Analysis ◽  
Thermoplastic Starch ◽  
High Rate ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Pilot Investigation ◽  
Nanosilica Particles ◽  
Xrd Techniques

This study is a pilot investigation on the effect of using nanosilica for reinforcing thermoplastic starch-based bioplastic films. An arbitrary 0.2[Formula: see text]wt.% of nanosilica particles were used to reinforce starch derived bioplastic materials and were further investigated for potential benefits. Nanosilica was extracted from rice husk and was characterized using methods like Fourier transform infrared spectroscopy (FTIR) technique and Brunauer–Emmett–Teller (BET) method. Transmission electron microscopy (TEM) and X-ray diffraction (XRD) techniques were used to determine the structure of nanosilica crystals. Scanning electron microscopy (SEM) technique was used to study the surface topography and composition of nano ‘silica. Both raw and reinforced bioplastic films were tested for thermal stability using thermo gravimetric analysis (TGA) and differential scanning calorimetry (DSC) tests and their performance was compared. Mechanical properties were compared using tensile and tear tests and biodegradability was assessed through enzymatic degradation analysis. It was found that the presence of nanosilica improved the bonding of polymer matrix and in turn increased the thermal stability and tear strength. Nanosilica reinforced matrix resulted in the increase of surface area than raw bioplastic matrix, which lead to high rate of enzymatic reactivity and degradation rate.

scholarly journals Structural, Thermal, Morphological, Adsorption and Catalytic Properties of Poly(BPDAH-co-ODA/PPDA)-Ag/V2O5 Nanocomposites

2019 ◽  
Vol 15 (1) ◽  
pp. 155-174
Author(s):  
Govindharajan Sribala ◽  
Balakrishnan Meenarathi ◽  
Ramasamy Anbarasan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
1H Nmr ◽  
Catalytic Reduction ◽  
Aromatic Proton ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Transmission Electron ◽  
Ft Ir ◽  
Scanning Electron

Thermally stable polyimides (PIs) were prepared by condensation technique at 160 ºC for 5 hours in N-methylpyrrolidone (NMP) medium under N2 atmosphere both in the presence and absence of metal (Ag) and metaloxide (MO) (V2O5) nanoparticles (NPs). The synthesized polymers are characterized by Fourier Transform Infra Red (FT-IR) spectroscopy, 1H Nuclear Magnetic Resonance (1H NMR) spectroscopy, Differential Scanning Calorimetry (DSC), Thermal Gravimetric Analysis (TGA), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Field Emission Scanning Electron Microscopy with Energy Dispersive X-Ray (FE-SEM and EDX). The FT-IR spectrum showed a peak at 1786 cm-1 corresponding to the C=O stretching of dianhydride. The aromatic proton signals appeared between 6.7 and 7.5 ppm in the 1H-NMR spectrum of the resultant PIs. The oxydianiline (ODA) based PI with Ag NP loaded system exhibited the highest Tg value. The apparent rate constant values for the adsorption and catalytic reduction of p-nitrophenol (PNP), Cr6+ and rhodamine 6G (R6G) dye were determined with the help of UV-visible spectrophotometer. Among the catalysts, the system loaded with V2O5 NP has higher kapp values. The experimental results are critically analyzed and compared with the previously available literature values. Copyright © 2020 BCREC Group. All rights reserved 

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