scholarly journals Intermittent Compressive Stress Enhanced Insulin-Like Growth Factor-1 Expression in Human Periodontal Ligament Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Jittima Pumklin ◽  
Jeeranan Manokawinchoke ◽  
Kanokporn Bhalang ◽  
Prasit Pavasant
Keyword(s):  
Mechanical Stress ◽  
Compressive Stress ◽  
Periodontal Ligament ◽  
Molecular Mechanisms ◽  
Treated Group ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells ◽  
Chemical Inhibitors

Mechanical force was shown to promote IGF-1 expression in periodontal ligament both in vitro and in vivo. Though the mechanism of this effect has not yet been proved, here we investigated the molecular mechanism of intermittent mechanical stress on IGF-1 expression. In addition, the role of hypoxia on the intermittent compressive stress on IGF-1 expression was also examined. In this study, human periodontal ligament cells (HPDLs) were stimulated with intermittent mechanical stress for 24 hours. IGF-1 expression was examined by real-time polymerase chain reaction. Chemical inhibitors were used to determine molecular mechanisms of these effects. For hypoxic mimic condition, the CoCl2 supplementation was employed. The results showed that intermittent mechanical stress dramatically increased IGF-1 expression at 24 h. The pretreatment with TGF-β receptor I or TGF-β1 antibody could inhibit the intermittent mechanical stress-induced IGF-1 expression. Moreover, the upregulation of TGF-β1 proteins was detected in intermittent mechanical stress treated group. Correspondingly, the IGF-1 expression was upregulated upon being treated with recombinant human TGF-β1. Further, the hypoxic mimic condition attenuated the intermittent mechanical stress and rhTGF-β1-induced IGF-1 expression. In summary, this study suggests intermittent mechanical stress-induced IGF-1 expression in HPDLs through TGF-β1 and this phenomenon could be inhibited in hypoxic mimic condition.

scholarly journals Panax ginseng Fruit Has Anti-Inflammatory Effect and Induces Osteogenic Differentiation by Regulating Nrf2/HO-1 Signaling Pathway in In Vitro and In Vivo Models of Periodontitis

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1221
Author(s):  
Eun-Nam Kim ◽  
Tae-Young Kim ◽  
Eui Kyun Park ◽  
Jae-Young Kim ◽  
Gil-Saeng Jeong
Keyword(s):  
Panax Ginseng ◽  
Periodontal Ligament ◽  
Therapeutic Agent ◽  
Alveolar Bone ◽  
Periodontal Ligament Cells ◽  
In Vivo Models ◽  
Human Periodontal Ligament Cells ◽  
Anti Inflammatory

Periodontitis is an infectious inflammatory disease of tissues around teeth that destroys connective tissues and is characterized by the loss of periodontal ligaments and alveolar bone. A new treatment strategy is needed owing to the limitations of the current surgical treatment method and the side effects of anti-inflammatory drugs. Therefore, here, we assessed whether Panax ginseng fruit extract (PGFE) is a new therapeutic agent for periodontitis in vitro and in vivo. According to the results, PGFE suppressed pro-inflammatory cytokines such as tumor necrosis factor-α, interleukin (IL)-1β, and IL-6, and pro-inflammatory mediators such as inducible nitric oxide synthase and cyclooxygenase-2 through heme oxygenase-1 expression in human periodontal ligament cells stimulated with Porphyromonas gingivalis lipopolysaccharide (PG-LPS). In addition, the osteogenic induction of human periodontal ligament cells was inhibited by PG-LPS, and protein and mRNA levels of osteogenic markers such as alkaline phosphatase, collagen type 1 (COL1), osteopontin (OPN), and runt-related transcription factor 2 (RUNX2) were increased. The efficacy of PGFE for inhibiting periodontitis in vitro was demonstrated in a representative in vitro model of periodontitis induced by ligature and PG-LPS. Subsequently, hematoxylin and eosin staining and micro-computed tomography of the euthanized experimental animal model confirmed suppressed periodontal inflammation, which is an important strategy for treating periodontitis and for recovering the resulting alveolar bone loss. Therefore, PGFE is a potential, novel therapeutic agent for periodontal diseases.

Potential of the propolis as storage medium to preserve the viability of cultured human periodontal ligament cells: an in vitro study

2011 ◽  
Vol 27 (2) ◽  
pp. 102-108 ◽  
Author(s):  
Payal Saxena ◽  
Vandana Aditya Pant ◽  
Kulvindar Kaur Wadhwani ◽  
Mahendra Pratap Kashyap ◽  
Saurabh Kumar Gupta ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
In Vitro Study ◽  
Vitro Study ◽  
Periodontal Ligament Cells ◽  
Storage Medium ◽  
Human Periodontal Ligament Cells

Functional Behaviour of Human Periodontal Ligament Cells around Various Dental Implants

2007 ◽  
Vol 361-363 ◽  
pp. 837-840 ◽  
Author(s):  
Xiao Ting Luo ◽  
Zhen Gao ◽  
Shi Gui Yan ◽  
Wei Deng ◽  
Wen Shu Zhang ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Dental Implants ◽  
Periodontal Ligament ◽  
Cell Counting ◽  
Immunofluorescent Staining ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells ◽  
Proliferation And Differentiation ◽  
In Vitro Systems

In the present investigation, four titanium (Ti) surfaces of dental implants were compared through in vitro systems. The surface roughness of Ti was measured by TR240 mobile surface roughmeter. The Ti implants were seeded with human periodontal ligament cells (hPLDCs) and maintained for a period of 0-7 days. The adhesion, proliferation, and differentiation of hPLDCs were observed by using Cell morphology, cell counting and Osteocalcin (OC) immunofluorescent staining. Results suggest that surface roughness of titanium favors hPDLCs behavior and improves cell adhesion, proliferation, and differentiation.

scholarly journals Gold Nanoparticles Combined Human β-Defensin 3 Gene-Modified Human Periodontal Ligament Cells Alleviate Periodontal Destruction via the p38 MAPK Pathway

2021 ◽  
Vol 9 ◽  
Author(s):  
Lingjun Li ◽  
Yangheng Zhang ◽  
Min Wang ◽  
Jing Zhou ◽  
Qian Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Osteogenic Differentiation ◽  
P38 Mapk ◽  
Periodontal Ligament ◽  
Mapk Pathway ◽  
Periodontal Regeneration ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells ◽  
P38 Mapk Pathway

Periodontitis is a chronic inflammatory disease with plaques as the initiating factor, which will induce the destruction of periodontal tissues. Numerous studies focused on how to obtain periodontal tissue regeneration in inflammatory environments. Previous studies have reported adenovirus-mediated human β-defensin 3 (hBD3) gene transfer could potentially enhance the osteogenic differentiation of human periodontal ligament cells (hPDLCs) and bone repair in periodontitis. Gold nanoparticles (AuNPs), the ideal inorganic nanomaterials in biomedicine applications, were proved to have synergetic effects with gene transfection. To further observe the potential promoting effects, AuNPs were added to the transfected cells. The results showed the positive effects of osteogenic differentiation while applying AuNPs into hPDLCs transfected by adenovirus encoding hBD3 gene. In vivo, after rat periodontal ligament cell (rPDLC) transplantation into SD rats with periodontitis, AuNPs combined hBD3 gene modification could also promote periodontal regeneration. The p38 mitogen-activated protein kinase (MAPK) pathway was demonstrated to potentially regulate both the in vitro and in vivo processes. In conclusion, AuNPs can promote the osteogenic differentiation of hBD3 gene-modified hPDLCs and periodontal regeneration via the p38 MAPK pathway.

Cyclic tensile force stimulates BMP9 synthesis and in vitro mineralization by human periodontal ligament cells

2018 ◽  
Vol 234 (4) ◽  
pp. 4528-4539 ◽  
Author(s):  
Yanee Tantilertanant ◽  
Jitti Niyompanich ◽  
Vincent Everts ◽  
Pitt Supaphol ◽  
Prasit Pavasant ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Tensile Force ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells ◽  
In Vitro Mineralization
Sign in / Sign up

Export Citation Format

Share Document