scholarly journals Analysis of Serum MicroRNAs as Potential Biomarker in Coronary Bifurcation Lesion

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Yan Liu ◽  
Shaoliang Chen ◽  
Junjie Zhang ◽  
Shoujie Shan ◽  
Liang Chen ◽  
...  
Keyword(s):  
Pcr Analysis ◽  
Circulating Mirnas ◽  
Coronary Bifurcation ◽  
Serum Samples ◽  
Diagnosis And Prognosis ◽  
Bifurcation Lesion ◽  
Potential Biomarker ◽  
Novel Biomarkers ◽  
Coronary Bifurcation Lesion ◽  
Serum Micrornas

Recent evidence suggests that cell-derived circulating miRNAs may serve as the biomarkers of cardiovascular diseases. However, no study has investigated the potential of circulating miRNAs as biomarker for coronary bifurcation lesion. In this study, we aimed to characterize the miRNA profiles that could distinguish coronary bifurcation lesion and identify potential miRNAs as biomarkers of coronary bifurcation lesion. We employed miRNA microarray to screen serum miRNAs profiles of patients with coronary bifurcation lesion and coronary nonbifurcation lesions. We identified 197 miRNAs differentially expressed, including 150 miRNAs upregulated and 47 miRNAs downregulated. We chose 3 miRNAs with significant differences for further testing in 200 patients. RT-PCR analysis of serum samples confirmed that miR30d was upregulated and miR1246 was downregulated in the serum of coronary bifurcation lesion patients compared with nonbifurcation lesion patients. Our findings suggest that these miRNAs may have a role in the pathogenesis of coronary bifurcation lesion and may represent novel biomarkers for the diagnosis and prognosis of coronary bifurcation lesion.

scholarly journals OPTIMAL STRATEGY FOR SIDE BRANCH STENTING IN CORONARY BIFURCATION LESION

2012 ◽  
Vol 59 (13) ◽  
pp. E84
Author(s):  
Young Bin Song ◽  
Joo–Yong Hahn ◽  
Jin–Ho Choi ◽  
Seung–Hyuk Choi ◽  
Hyeon–Cheol Gwon
Keyword(s):  
Optimal Strategy ◽  
Side Branch ◽  
Coronary Bifurcation ◽  
Bifurcation Lesion ◽  
Coronary Bifurcation Lesion

scholarly journals Circular RNA Expression Profiling Identifies Prostate Cancer- Specific circRNAs in Prostate Cancer

2018 ◽  
Vol 50 (5) ◽  
pp. 1903-1915 ◽  
Author(s):  
Qianlin Xia ◽  
Tao Ding ◽  
Guihong Zhang ◽  
Zehuan Li ◽  
Ling Zeng ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction Analysis ◽  
Circular Rna ◽  
Differentially Expressed ◽  
Pcr Analysis ◽  
High Morbidity ◽  
Qrt Pcr ◽  
Diagnosis And Prognosis ◽  
Novel Biomarkers ◽  
Rna Expression Profiling

Background/Aims: Prostate cancer (PCa) is one of the main cancers that damage males’ health severely with high morbidity and mortality, but there is still no ideal molecular marker for the diagnosis and prognosis of prostate cancer. Methods: To determine whether the differentially expressed circRNAs in prostate cancer can serve as novel biomarkers for prostate cancer diagnosis, we screened differentially expressed circRNAs using SBC-ceRNA array in 4 pairs of prostate tumor and paracancerous tissues. A circRNA-miRNA-mRNA regulatory network for the differential circRNAs and their host genes was constructed by Cytoscape3.5.1 software. Quantitative real-time polymerase chain reaction analysis (qRT-PCR) was performed to confirm the microarray data. Results: We found 1021 differentially expressed circRNAs in PCa tumor using SBC-ceRNA array and confirmed the expression of circ_0057558, circ_0062019 and SLC19A1 in PCa cell lines and tumor tissues through qRT-PCR analysis. We demonstrated that combination of PSA level and two differentially expressed circRNAs showed significantly increased AUC, sensitivity and specificity (0.938, 84.5% and 90.9%, respectively) than PSA alone (AUC of serum PSA was 0.854). Moreover, circ_0057558 was correlated positively with total cholesterol. The functional network of circRNA-miRNA-mRNA analysis showed that circ_0057558 and circ_0034467 regulated miR-6884, and circ_0062019 and circ_0060325 regulated miR-5008. Conclusion: Our results demonstrated that differentially expressed circRNAs (circ_0062019 and circ_0057558) and host gene SLC19A1 of circ_0062019 could be used as potential novel biomarkers for prostate cancer.

scholarly journals Identification and Characterization of Circulating MicroRNAs as Novel Biomarkers in Dogs With Heart Diseases

2021 ◽  
Vol 8 ◽  
Author(s):  
Woong-Bin Ro ◽  
Min-Hee Kang ◽  
Doo-Won Song ◽  
Heyong-Seok Kim ◽  
Ga-Won Lee ◽  
...  
Keyword(s):  
Roc Analysis ◽  
Heart Diseases ◽  
Ductus Arteriosus ◽  
Canine Heart ◽  
Circulating Mirnas ◽  
Serum Samples ◽  
Circulating Micrornas ◽  
Concentric Hypertrophy ◽  
Novel Biomarkers ◽  
Healthy Dogs

Background: Previous studies in humans have confirmed dysregulations of circulating microRNAs (miRNAs) in patients with various cardiovascular diseases. However, studies on circulating miRNAs in dogs with various heart diseases are limited in number. This study aimed to identify significantly dysregulated circulating miRNAs and characterize them as novel biomarkers in dogs with heart diseases.Materials and Methods: Circulating levels of 11 miRNAs were investigated in serum samples of 82 dogs (72 with heart diseases and 10 healthy dogs) using quantitative reverse transcription-polymerase chain reaction. The results were correlated to clinical data including echocardiographic results and N-terminal pro B-type natriuretic peptide (NT-proBNP) levels.Results: Upregulation of cfa-miR-130b was observed in dogs with myxomatous mitral valve degeneration (MMVD) stage B, patent ductus arteriosus, and pulmonic stenosis. In dogs with MMVD stage B, cfa-miR-130b was upregulated and correlated with clinical indices. In receiver operating characteristic (ROC) analysis, cfa-miR-130b accurately distinguished dogs with diseases from healthy dogs. We also observed that cfa-miR-375 and cfa-let-7b were upregulated in dogs with concentric cardiac hypertrophy. The cfa-miR-375 was correlated with concentric hypertrophy indices and was an accurate indicator of concentric hypertrophy in ROC analysis.Conclusions: The miRNAs identified in this study may be used as novel biomarkers and possible candidates for therapeutic targets in various canine heart diseases.

scholarly journals Coronary Bifurcation Lesion–Management

2020 ◽  
Vol 5 (02) ◽  
pp. 137-154
Author(s):  
Maddury Jyotsna
Keyword(s):  
Management Strategy ◽  
Cardiac Events ◽  
Coronary Bifurcation ◽  
Bifurcation Lesion ◽  
Adverse Cardiac Events ◽  
Drug Eluting ◽  
Bifurcation Lesions ◽  
Coronary Bifurcation Lesion ◽  
Modern Era

AbstractCoronary bifurcation lesion management is challenging even in this modern era of drug-eluting stents (DES). A debate always exists as to what mode of treatment is better–one-stent or two-stent strategy. As in hospital and major adverse cardiac events (MACE) are more in the follow-up, what improvements in management strategy are required in relation to bifurcation lesions? We have more questions than answers for this type of lesion. In this article, we try to analyze these issues.

scholarly journals Crush Stenting With Drug-Eluting Stents: Relevance of Coronary Bifurcation Lesion Location on Angiographic and Clinical Outcomes

2010 ◽  
Vol 33 (12) ◽  
pp. E32-E39 ◽  
Author(s):  
Shao-Liang Chen ◽  
Jun-jie Zhang ◽  
Fei Ye ◽  
Zhi-zhong Liu ◽  
Zhong-sheng Zhu ◽  
...  
Keyword(s):  
Clinical Outcomes ◽  
Drug Eluting Stents ◽  
Coronary Bifurcation ◽  
Lesion Location ◽  
Bifurcation Lesion ◽  
Drug Eluting ◽  
Coronary Bifurcation Lesion

AS-035 Novel Scoring Method of Plaque and Calcification by Intravascular Ultrasound in the Coronary Bifurcation Lesion

2012 ◽  
Vol 109 (7) ◽  
pp. S82
Author(s):  
Tomohiko Yamamoto ◽  
Yoshinobu Murasato ◽  
Kohei Takata ◽  
Yasunori Suematsu ◽  
Toru Higa ◽  
...  
Keyword(s):  
Intravascular Ultrasound ◽  
Coronary Bifurcation ◽  
Scoring Method ◽  
Bifurcation Lesion ◽  
Coronary Bifurcation Lesion

Treatment of a coronary bifurcation lesion with drug-coated balloons: lumen enlargement and plaque modification after 6 months

2013 ◽  
Vol 102 (6) ◽  
pp. 469-472 ◽  
Author(s):  
Bruno Scheller ◽  
Dieter Fischer ◽  
Yvonne P. Clever ◽  
Franz X. Kleber ◽  
Ulrich Speck ◽  
...  
Keyword(s):  
Coronary Bifurcation ◽  
Bifurcation Lesion ◽  
Coronary Bifurcation Lesion

scholarly journals Serum microRNAs are non-invasive biomarkers for the presence and progression of subarachnoid haemorrhage

2017 ◽  
Vol 37 (1) ◽  
Author(s):  
Nian-sheng Lai ◽  
Jia-qi Zhang ◽  
Fei-yun Qin ◽  
Bin Sheng ◽  
Xing-gen Fang ◽  
...  
Keyword(s):  
Subarachnoid Haemorrhage ◽  
Quantitative Pcr ◽  
Operating Characteristic ◽  
Roc Curves ◽  
Pcr Analysis ◽  
Healthy Controls ◽  
Modified Rankin Scale ◽  
Serum Samples ◽  
Non Invasive ◽  
Serum Micrornas

miRNAs are important regulators of translation and have been associated with the pathogenesis of a number of cardiovascular diseases including stroke and may be possible prognostic biomarkers. The purpose of the present study was to determine the expression levels of miRNAs in the sera of subarachnoid haemorrhage (SAH) patients and to evaluate their relationships with the severity and clinical outcome of SAH. Serum samples on day 3 after the onset of SAH were subjected to microarray analysis with Exqion miRCURYTM LNA array and quantitative PCR analysis. Serum samples from SAH patients (n=60) and healthy controls (n=10) were subjected to quantitative PCR analysis. The severities and clinical outcomes of the SAH patients were evaluated with the WFNS grade and the Modified Rankin Scale (mRS). Three miRNAs, miR-502-5p, miR-1297 and miR-4320 were significantly up-regulated in the sera of SAH patients when compared with the healthy controls. The serum miR-502-5p and miR-1297 levels were significantly higher in the patients with severe SAH and a poor outcome than in those with mild SAH and a good outcome (P<0.05). The areas under the receiver operating characteristic (ROC) curves (AUCs) of miR-502-5p, miR-1297 and miR-4320 to distinguish the SAH patients from the healthy controls were 0.958 (P<0.001), 0.950 (P<0.001) and 0.843 (P<0.001) respectively. Taken together, these results indicate that miR-502-5p and miR-1297 are potentially valuable indicators of the diagnosis, severity and prognosis of SAH, and miR-4320 was a potentially valuable indicator of the diagnosis of SAH.

scholarly journals Serum MicroRNA Profiles Serve as Novel Biomarkers for the Diagnosis of Alzheimer’s Disease

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Hui Dong ◽  
Jialu Li ◽  
Lei Huang ◽  
Xi Chen ◽  
Donghai Li ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Characteristic Curve ◽  
Serum Samples ◽  
Individual Level ◽  
Serum Microrna ◽  
Serum Mirnas ◽  
Potential Biomarker ◽  
Novel Biomarkers ◽  
The Individual

Alzheimer’s disease (AD) is the most common type of dementia, and promptly diagnosis of AD is crucial for delaying the development of disease and improving patient quality of life. However, AD detection, particularly in the early stages, remains a substantial challenge due to the lack of specific biomarkers. The present study was undertaken to identify and validate the potential of circulating miRNAs as novel biomarkers for AD. Solexa sequencing was employed to screen the expression profile of serum miRNAs in AD and controls. RT-qPCR was used to confirm the altered miRNAs at the individual level. Moreover, candidate miRNAs were examined in the serum samples of patients with mild cognitive impairment (MCI) and vascular dementia (VD). The results showed that four miRNAs (miR-31, miR-93, miR-143, and miR-146a) were markedly decreased in AD patients’ serum compared with controls. Receiver operating characteristic curve analysis demonstrated that this panel of four miRNAs could be used as potential biomarker for AD. Furthermore, miR-93, and miR-146a were significantly elevated in MCI compared with controls, and the panel of miR-31, miR-93 and miR-146a can be used to discriminate AD from VD. We established a panel of four serum miRNAs as a novel noninvasive biomarker for AD diagnosis.

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