scholarly journals Traditional Chinese Medicine, the Zishen Yutai Pill, Ameliorates Precocious Endometrial Maturation Induced by Controlled Ovarian Hyperstimulation and Improves Uterine Receptivity via Upregulation of HOXA10

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Qi Gao ◽  
Lu Han ◽  
Xiumei Li ◽  
Xia Cai
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Target Gene ◽  
Controlled Ovarian Hyperstimulation ◽  
Reproductive Technology ◽  
Ovarian Hyperstimulation ◽  
Uterine Receptivity ◽  
Rt Pcr ◽  
Implantation Rates ◽  
Important Marker

Controlled ovarian hyperstimulation (COH) is widely used in assisted reproductive technology (ART), but it often leads to precocious maturation of the endometrium such that it impairs embryonic implantation and limits pregnancy rates. Previous studies have shown the traditional Chinese medicine, the Zishen Yutai pill (ZYP), to be effective in treatment of threatened as well as recurrent miscarriages, and it can improve embryonic implantation rates in patients undergoing IVF treatment. In the present study, the ZYP has been found to ameliorate precocious endometrial maturation in a mouse model of different COH. Molecular evaluations, real-time PCR, relative RT-PCR, Western blotting, and immunohistochemistry have indicated that the ZYP increased the expression of HOXA10, an important marker of uterine receptivity. Elevation of HOXA10 led to further upregulation of its target gene, integrinβ3, and downregulation of EMX2, two additional markers of uterine receptivity. In this way, the ZYP may mitigate COH-induced precocious maturation of the endometrium and improve uterine receptivity by upregulating HOXA10.

Ovarian hyperstimulation affects fluid transporters in the uterus: a potential mechanism in uterine receptivity

2014 ◽  
Vol 26 (7) ◽  
pp. 982 ◽  
Author(s):  
Laura A. Lindsay ◽  
Christopher R. Murphy
Keyword(s):  
Implantation Rate ◽  
Normal Pregnancy ◽  
Controlled Ovarian Hyperstimulation ◽  
Fertility Treatment ◽  
Ovarian Hyperstimulation ◽  
Potential Mechanism ◽  
Uterine Receptivity ◽  
Aquaporin 5 ◽  
Uterine Fluid ◽  
Luminal Epithelial Cells

Controlled ovarian hyperstimulation is commonly used in fertility treatment. Evidence suggests that this could alter the endometrial environment and influence implantation rate. However, the mechanisms underlying this disruption are unknown. A recently developed rat ovarian hyperstimulation (OH) model found alterations in the localisation and expression of several molecules associated with implantation, as well as an increase in luminal fluid at the time of implantation. The present study investigated the effects of OH in rats on the expression of fluid-transporting molecules aquaporin 5 (AQP5) and claudin 4. The expression of these proteins was investigated in uterine luminal epithelial cells of rats undergoing OH and compared with normal pregnancy. There was a significant increase in AQP5 protein in OH rats at the time of implantation, along with a loss of the mesometrial staining gradient, which is thought to contribute to implantation position. At the same time, there was a significant decrease in claudin 4 protein. These results suggest that OH in rats causes a dysregulation in uterine fluid dynamics through modifications to fluid-transporting molecules, resulting in an unfavourable implantation environment for the blastocyst.

scholarly journals A Network Pharmacology-Based Study on Irritable Bowel Syndrome Prevention and Treatment Utilizing Shenling Baizhu Powder

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Meng Meng ◽  
Chen Bai ◽  
Bo Wan ◽  
Luqing Zhao ◽  
Zhe Li ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Irritable Bowel Syndrome ◽  
Molecular Docking ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Signaling Pathway ◽  
Target Gene ◽  
Network Pharmacology ◽  
The Core ◽  
Irritable Bowel

Background and Objective. Irritable bowel syndrome (IBS) is a prevalent disorder of the gastrointestinal system with complex pathogenesis. Shenling Baizhu powder (SLBZP) is a Chinese herbal compound with multicomponent and multitarget characteristics. Increasing volumes of evidence demonstrate that it has a notable therapeutic impact on IBS. This study therefore is aimed at exploring the potential effective components of SLBZP and their mechanisms in IBS treatment utilizing network pharmacology. Methods. Metabolomics was used to detect the secondary metabolites in SLBZP; the target protein was acquired by target fishing according to the compound’s structure. The SymMap database was used to search herbal medicines for the target protein. The target gene of IBS gave rise to the common gene protein which is the potential target of SLBZP in IBS therapy. The interactions between target proteins were analyzed in a STRING database, the protein relationship network was analyzed using Cytoscape software, and the Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the core target gene group was carried out in a DAVID database in order to construct the “compound-traditional Chinese medicine/molecule-target-pathway” network. Molecular docking was used to verify the core protein and its related small molecular compounds. Result. There were 129 types of secondary metabolites in SLBZP. 80 target proteins of these metabolites were potential core targets for IBS treatment including acetylcholinesterase (AChE), arachidonate-5-lipoxygenase (ALOX5), B-cell lymphoma-2 (BCL2), recombinant cyclin D1 (CCND1), and catenin-β1 (CTNNB1), among others. Results from these targets indicated that the most enriched pathway was the tumor necrosis factor (TNF) signaling pathway ( p < 0.001 ) and that the most abundant pathway was signal transduction. In the network nodes of the TNF signaling pathway, the Chinese medicines with the highest aggregation were Lablab semen album and Glycyrrhizae radix et rhizoma ( degree = 11 ). The small molecules with the highest aggregation were oxypeucedanin and 3,5,6,7,8,3 ′ ,4 ′ -heptamethoxyflavone ( degree = 4 ). Molecular docking results confirmed that daidzein 7-O-glucoside (daidzin) had the highest degree of binding to TNF proteins in the TNF signaling pathway. Conclusion. This study shows that SLBZP can treat IBS by influencing multiple targets and pathways, of which the TNF signaling pathway may be the most significant. This typifies the pharmacological characteristics of traditional Chinese medicine, i.e., multiple targets, numerous pathways, and specific therapeutic effects on diseases. SLBZP can therefore be used as a candidate drug for clinical IBS by intervening in human signal transduction.

The Effects of Five Alkaloids from Bulbus Fritillariae on the Concentration of cAMP in HEK Cells Transfected with Muscarinic M2 Receptor Plasmid

2006 ◽  
Vol 34 (05) ◽  
pp. 901-910 ◽  
Author(s):  
Ying Zhou ◽  
Hui Ji ◽  
Bao-Qin Lin ◽  
Yan Jiang ◽  
Ping Li
Keyword(s):  
Chinese Medicine ◽  
Calcium Phosphate ◽  
Traditional Chinese Medicine ◽  
Western Blot ◽  
Camp Level ◽  
Rt Pcr ◽  
Camp Concentration ◽  
Hek Cells ◽  
Co Precipitation

The aim of this study was to investigate the effects of five alkaloids, namely verticine, verticinone, imperialine, imperialine-3β-D-glucoside, and puqietinone, purified from Bulbus Fritillariae and used as an antitussive drug in traditional Chinese medicine, on their antimuscarinic M2 function and the cAMP level of HEK cells transfected with muscarinic M2 receptor plasmid. By transfecting the HEK cells with the method of calcium phosphate co-precipitation and screening with G418, the cells stably expressing M2 receptor were identified. The expression of M2 receptor in HEK cells was confirmed by both RT-PCR and western blot. The cAMP level in the treated cells was analyzed with RIA method (125I-cAMP KIT). And the results suggested that the five alkaloids could significantly elevate the cAMP concentration in the HEK cells transfected with muscarinic M2 receptor plasmid ( p < 0.01).

scholarly journals Systems Pharmacology-Based Approach of Connecting Disease Genes in Genome-Wide Association Studies with Traditional Chinese Medicine

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Jihye Kim ◽  
Minjae Yoo ◽  
Jimin Shin ◽  
Hyunmin Kim ◽  
Jaewoo Kang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Target Gene ◽  
Target Genes ◽  
Disease Gene ◽  
Drug Repurposing ◽  
Genome Wide Association Studies ◽  
Systems Pharmacology ◽  
Potential Drug ◽  
Gene Pairs

Traditional Chinese medicine (TCM) originated in ancient China has been practiced over thousands of years for treating various symptoms and diseases. However, the molecular mechanisms of TCM in treating these diseases remain unknown. In this study, we employ a systems pharmacology-based approach for connecting GWAS diseases with TCM for potential drug repurposing and repositioning. We studied 102 TCM components and their target genes by analyzing microarray gene expression experiments. We constructed disease-gene networks from 2558 GWAS studies. We applied a systems pharmacology approach to prioritize disease-target genes. Using this bioinformatics approach, we analyzed 14,713 GWAS disease-TCM-target gene pairs and identified 115 disease-gene pairs with q value < 0.2. We validated several of these GWAS disease-TCM-target gene pairs with literature evidence, demonstrating that this computational approach could reveal novel indications for TCM. We also develop TCM-Disease web application to facilitate the traditional Chinese medicine drug repurposing efforts. Systems pharmacology is a promising approach for connecting GWAS diseases with TCM for potential drug repurposing and repositioning. The computational approaches described in this study could be easily expandable to other disease-gene network analysis.

Sign in / Sign up

Export Citation Format

Share Document