scholarly journals Structural Characterization andIn VitroAntioxidant Activity of Kojic Dipalmitate Loaded W/O/W Multiple Emulsions Intended for Skin Disorders

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Maíra Lima Gonçalez ◽  
Diana Gleide Marcussi ◽  
Giovana Maria Fioramonti Calixto ◽  
Marcos Antonio Corrêa ◽  
Marlus Chorilli
Keyword(s):  
Antioxidant Activity ◽  
Safety Profile ◽  
Flow Behavior ◽  
Microscopic Analysis ◽  
Skin Disorders ◽  
Activity Assay ◽  
Multiple Emulsions ◽  
Lipophilic Antioxidant ◽  
Newtonian Behavior

Multiple emulsions (MEs) are intensively being studied for drug delivery due to their ability to load and increase the bioavailability of active lipophilic antioxidant, such as kojic dipalmitate (KDP). The aim of this study was to structurally characterize developed MEs by determining the average droplet size (Dnm) and zeta potential (ZP), performing macroscopic and microscopic analysis and analyzing their rheological behavior andin vitrobioadhesion. Furthermore, thein vitrosafety profile and antioxidant activity of KDP-loaded MEs were evaluated. The developed MEs showed a Dnm of approximately 1 micrometer and a ZP of −13 mV, and no change was observed in Dnm or ZP of the system with the addition of KDP. KDP-unloaded MEs exhibited ‘‘shear thinning’’ flow behavior whereas KDP-loaded MEs exhibited Newtonian behavior, which are both characteristic of antithixotropic materials. MEs have bioadhesion properties that were not influenced by the incorporation of KDP. The results showed that the incorporation of KDP into MEs improved the safety profile of the drug. Thein vitroantioxidant activity assay suggested that MEs presented a higher capacity for maintaining the antioxidant activity of KDP. ME-based systems may be a promising platform for the topical application of KDP in the treatment of skin disorders.

Recent Applications for in Vitro Antioxidant Activity Assay

2016 ◽  
Vol 46 (5) ◽  
pp. 389-399 ◽  
Author(s):  
Andrei A. Bunaciu ◽  
Andrei Florin Danet ◽  
Şerban Fleschin ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Antioxidant Activity ◽  
Activity Assay ◽  
In Vitro Antioxidant Activity

scholarly journals In vitro Antioxidant and Anti-obesity Activities of Freeze-dried Canarium sp., Averrhoa bilimbi L. and Malus domestica

2019 ◽  
Vol 11 (3) ◽  
pp. 320-6
Author(s):  
Sri Utami ◽  
Susi Endrini ◽  
Said Nafik ◽  
Intan Meila Tria Lestari ◽  
Dea Anindya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Malus Domestica ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Activity Assay ◽  
Flavonoid Content ◽  
Dried Fruits ◽  
Hydroxycitric Acid ◽  
Freeze Dried

BACKGROUND: The number of obesity cases is still increasing worldwide and has reached an epidemic scale. Plants are known to have a protection role in the development of obesity, however their antioxidant and anti-obesity activities have not widely known. This study was conducted to assess the in vitro antioxidant and anti-obesity activities of different types of freeze-dried fruits cultivated in Indonesia, especially Canarium sp., Averrhoa bilimbi L. and Malus domestica.METHODS: Total phenolic content of freeze-dried fruits was identified by Folin-Ciocalteu method, while the total flavonoid content was measured by aluminium chloride colorimetric assay. To assessed the antioxidant activity, 2,2-diphenyl 1-picrylhydrazyl (DPPH) scavenging activity assay and 2,2’-azino-bis (3-ethylbenzothiazoline6-sulphonic acid) (ABTS) reducing activity assay were performed. The α-amylase and lipase inhibitory activity assay were performed to assess the anti-obesity activity. For comparison, hydroxycitric acid (HCA) compound was also assessed with DPPH, ABTS, α-amylase and lipase assays.RESULTS: A. bilimbi had the highest total phenol content (6.35 µg GAE/mg), meanwhile M. domestica had the highest total flavonoid content (2.06 µg QE/mg). A. bilimbi also showed the highest antioxidant activity both in DPPH and ABTS assay, with inhibitory concentration (IC50)=279.99 µg/mL and 631.78 µg/mL, respectively. The freeze-dried M. domestica had the highest anti-α-amylase activity (IC50=258.85 µg/mL), while Canarium sp. had the highest anti-lipase activity (IC50=118.66 µg/mL).CONCLUSION: Freeze-dried fruits demonstrate in vitro benefits toward obesity. A. bilimbi has potent antioxidant activity and is beneficial against obesity-related adverse health effect by relieving oxidative stress. M. domestica and Canarium sp. hamper the fat accumulation by reducing the carbohydrate absorption and dietary lipid.KEYWORDS: antioxidant, anti-obesity, Canarium sp., Averrhoa bilimbi L., Malus domestica, hydroxycitric acid

scholarly journals Topical Application of Retinyl Palmitate-Loaded Nanotechnology-Based Drug Delivery Systems for the Treatment of Skin Aging

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Marcela B. Oliveira ◽  
Alice Haddad do Prado ◽  
Jéssica Bernegossi ◽  
Claudia S. Sato ◽  
Iguatemy Lourenço Brunetti ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Topical Application ◽  
Safety Profile ◽  
Liquid Crystalline ◽  
Polarized Light ◽  
Liquid System ◽  
Skin Aging ◽  
Retinyl Palmitate ◽  
Dpph Method

The objective of this study was to perform a structural characterization and evaluate thein vitrosafety profile andin vitroantioxidant activity of liquid crystalline systems (LCS) with and without retinyl palmitate (RP). LCS containing polyether functional siloxane (PFS) as a surfactant, silicon glycol copolymer (SGC) as oil phase, and water in the ratios 30 : 25 : 45 and 40 : 50 : 10 with (OLSv= RP-loaded opaque liquid system andTLSv= RP-loaded transparent liquid system, respectively) and without (OLS and TLS, respectively) RP were studied. Samples were characterized using polarized light microscopy (PLM) and rheology analysis.In vitrosafety profile was evaluated using red cell hemolysis andin vitrocytotoxicity assays.In vitroantioxidant activity was performed by the DPPH method. PLM analysis showed the presence of lamellar LCS just to TLS. Regardless of the presence of RP, the rheological studies showed the pseudoplastic behavior of the formulations. The results showed that the incorporation of RP in LCS improved the safety profile of the drug.In vitroantioxidant activity suggests that LCS presented a higher capacity to maintain the antioxidant activity of RP. PFS-based systems may be a promising platform for RP topical application for the treatment of skin aging.

Enhancement of Antioxidant Activity by the Combination of Moringa oleifera and Perilla frutescens Seed Oils in Microemulsion

2020 ◽  
Vol 859 ◽  
pp. 100-106
Author(s):  
Prakairat Tunit ◽  
Somboon Kietinun ◽  
Kusuma Sriyakul ◽  
Parunkul Tungsukruthai ◽  
Chuda Chittasupho
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Moringa Oleifera ◽  
Flow Behavior ◽  
Physical Stability ◽  
Antioxidant Properties ◽  
Perilla Frutescens ◽  
Microemulsion System ◽  
Seed Oils

The aim of this study was to investigate the synergistic antioxidant activity of microemulsion containing mixed Moringa oleifera seed oil and Perilla frutescens seed oils, compared with microemulsion of single oils. The novel microemulsions for the moringa, perilla, and mixed seed oils were formulated by mixing appropriate amount of surfactant, water, and oil phase. The formulation containing the maximum amount of oil which appeared transparent was further evaluated for particle size, size distribution, surface charge, pH, and rheological behavior. The in vitro antioxidant properties of microemulsions were investigated in comparison with essential oils. Cream containing microemulsion was prepared and its physical stability was investigated by heating-cooling cycles. The results showed that the maximum content of oil incorporated in microemulsion system was 12.5%. The mean droplet sizes of moringa, perilla, and mixed oil microemulsion were 159.33±0.77, 183.86±1.42, and 263.43± 9.40 nm, respectively. All formulations exhibited pseudoplastic flow behavior. The mixture of moringa and perila seed oils in microemulsion possessed the highest significant antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl compared with single oil microemulsion. The cream containing microemulsion exhibited good physical stability. Thus, the current research reveals the benefits of microemulsion containing mixed moringa and perilla seed oils based cream compared with single essential oils.

In vitro antioxidant activity and tannin content of Echium italicum L.

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
N Niciforovic ◽  
S Solujic ◽  
V Mihailovic ◽  
D Pavlovic-Muratspahic
Keyword(s):  
Antioxidant Activity ◽  
Tannin Content ◽  
In Vitro Antioxidant Activity

In vitro cytotoxic and antioxidant activity of some Hypericum species belonging to drosanthe section

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
E Eroğlu Özkan ◽  
N Özsoy ◽  
G Özhan ◽  
A Mat
Keyword(s):  
Antioxidant Activity ◽  
Hypericum Species
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