scholarly journals Analysis of the Expression and Polymorphism ofAPOE, HSP, BDNF,andGRIN2BGenes Associated with the Neurodegeneration Process in the Pathogenesis of Primary Open Angle Glaucoma

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Alicja Nowak ◽  
Ireneusz Majsterek ◽  
Karolina Przybyłowska-Sygut ◽  
Dariusz Pytel ◽  
Katarzyna Szymanek ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Nerve Fiber ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Blood Analysis ◽  
Specific Gene ◽  
Qrt Pcr ◽  
Pcr Rflp ◽  
Allele Specific ◽  
Peripheral Blood Analysis

Glaucoma is characterized by optic neuropathy of the RGC or retinal nerve fiber. The aim of this study was to evaluate a relationship between the neurodegenerative genes’ polymorphisms of theAPOE(rs449647),BDNF(rs2030324),GRIN2B(rs3764028), andHSP70-1(rs1043618) and the occurrence risk of POAG and to investigate its effect on allele-specific gene expression. Genomic DNA was extracted from peripheral blood. Analysis of the genes’ polymorphisms was performed using PCR-RFLP. The level of mRNA expression was determined by QRT-PCR. We showed a statistically significant association ofBDNFandAPOEgenes’ polymorphisms with a risk of POAG occurrence. There was a statistically significant association of the rs2030324 polymorphism with progression of POAG based on cup disc ratio value and rs1043618 polymorphism based on nerve fiber index and rim area. Furthermore, we found that meanHSP70-1mRNA expression was significantly lower in the case of individuals with the G/G genotype than in the case of minor allele carriers, that is, G/C and C/C. We also found thatBDNFandHSP70-1expression level are associated with the progression of POAG based on rim area value. In conclusion, our results suggest thatBDNF,APOE, andHSP70-1genes might be associated with a risk of POAG occurrence in the Polish population.

Evaluation of dynamic thiol–disulfide homeostasis in glaucoma patients and the correlation with retinal nerve fiber layer analysis

2019 ◽  
Vol 30 (4) ◽  
pp. 690-699 ◽  
Author(s):  
Bayram Gulpamuk ◽  
Ufuk Elgin ◽  
Emine Sen ◽  
Pelin Yilmazbas ◽  
Salim Neselioglu ◽  
...  
Keyword(s):  
Ocular Hypertension ◽  
Nerve Fiber ◽  
Retinal Nerve Fiber Layer ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Open Angle ◽  
Fiber Layer ◽  
Nerve Fiber Layer ◽  
Total Thiol ◽  
Retinal Nerve Fiber

Purpose: The aim of this study was to evaluate thiol/disulfide homeostasis and ischemia-modified albumin levels with primary open-angle glaucoma, ocular hypertension, and control group; also to interpret the correlation between these biochemical parameters and retinal nerve fiber layer analysis. Material and methods: In a prospective cross-sectional study, 30 primary open-angle glaucoma cases, 30 ocular hypertension cases, and 30 control subjects were included in the study. Native thiol, total thiol, and disulfide measurements and disulfide/native thiol, disulfide/total thiol, and native thiol/total thiol ratios were evaluated as thiol–disulfide homeostasis. Albumin and ischemia-modified albumin parameters were also evaluated. All cases underwent detailed ophthalmologic examination including visual acuity, retinal nerve fiber layer via optical coherence tomography, intraocular pressure, and central corneal thickness measurements and visual field analysis by 24-2 Swedish Interactive Threshold Algorithm (SITA) Standard visual field test. Results: Primary open-angle glaucoma group had significantly higher ischemia-modified albumin values than ocular hypertension and control group (p < 0.001). Native thiol and total thiol values of control group were statistically higher than those of primary open-angle glaucoma and ocular hypertension groups. The correlation between the temporal retinal nerve fiber layer value and ischemia-modified albumin, disulfide/native thiol, and disulfide/total thiol values of the primary open-angle glaucoma patients included in the study was moderate correlation in negative direction (r = –0.46, r = –0.39, r = –0.39, respectively), whereas there was a statistically significant moderate correlation in positive direction between the native thiol/total thiol values (r = 0.39) (p < 0.05). Conclusion: These findings have reinforced the role of oxidative stress in the etiopathogenesis of primary open-angle glaucoma, suggesting that the thinning retinal nerve fiber layer may be associated with oxidative stress in favor of prooxidant shift.

Correlation between retinal nerve fiber layer thickness and IOP variation in glaucoma suspects and patients with primary open-angle glaucoma

2020 ◽  
pp. 112067212095758
Author(s):  
Sebastião Cronemberger ◽  
Artur W Veloso ◽  
Christy Veiga ◽  
Gustavo Scarpelli ◽  
Yara C Sasso ◽  
...  
Keyword(s):  
Nerve Fiber ◽  
Layer Thickness ◽  
Retinal Nerve Fiber Layer ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Open Angle ◽  
Fiber Layer ◽  
Nerve Fiber Layer ◽  
Nerve Fiber Layer Thickness ◽  
Inferior Quadrant

Purpose: To analyze the relationship between retinal nerve fiber layer thickness (RNFLT) and intraocular pressure (IOP) variation in glaucoma suspects (GS) and patients with primary open-angle glaucoma (POAG). Methods: Thirty-one GS and 34 POAG patients underwent ophthalmologic examination and 24-h IOP measurements. GS had IOPs ranging from 19 to 24 mmHg and/or suspicious appearance of the optic nerve. POAG patients had reproducible abnormal visual fields. We only included patients who presented with short-term IOP fluctuation >6 mm Hg (∆IOP). Only one eye per patient was included through a randomized process. Peripapillary RNFLT was assessed by spectral-domain optical coherence tomography. We correlated RNFLT with IOP parameters. Results: Mean IOP was similar between GS and POAG groups (15.6 ± 3.47 vs 15.6 ± 2.83 mmHg, p = 0.90) as was IOP peak at 6 AM (21.7 ± 3.85 vs 21.3 ± 3.80 mmHg, p = 0.68). Statistically significant negative correlations were found in POAG group between IOP at 6 AM and RNFLT in global ( rs = −0.543; p < 0.001), inferior ( rs = −0.540; p < 0.001), superior ( rs = −0.405; p = 0.009), and nasal quadrants ( rs = −0.561; p < 0.001). Negative correlations were also found between ∆IOP and RNFLT in global ( rs = −0.591; p < 0.001), and all other sectors ( p < 0.05). In GS IOP at 6 AM correlated only with inferior quadrant ( rs = −0.307; p = 0.047). Conclusion: IOP at 6 AM and ∆IOP had negative correlations with RNFLT quadrants in POAG. In GS this correlation occurred between IOP at 6 AM and inferior quadrant. These findings may indicate potential risk factors for glaucoma progression.

Pattern electroretinogram changes in patients with primary open-angle glaucoma in correlation with visual field and optical coherence tomography changes

2019 ◽  
Vol 30 (6) ◽  
pp. 1362-1369 ◽  
Author(s):  
Asmaa M Elgohary ◽  
Hazem A Elbedewy ◽  
Hisham A Saad ◽  
Tarek M Eid
Keyword(s):  
Nerve Fiber ◽  
Retinal Nerve Fiber Layer ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Pattern Electroretinogram ◽  
Average Thickness ◽  
Open Angle ◽  
Fiber Layer ◽  
Nerve Fiber Layer ◽  
Retinal Nerve Fiber

Purpose: To study the pattern electroretinogram changes in primary open-angle glaucoma patients in correlation with visual field changes and optical coherence tomography measurements of retinal nerve fiber layer thickness in the peripapillary region in an attempt to evaluate the clinical value of pattern electroretinogram as an objective test of functional deficit in glaucoma. Patients and Methods: The study included 81 eyes of 81 participants: 50 primary open-angle glaucoma patients, 16 primary open-angle glaucoma suspects, and 15 controls. All subjects underwent visual field testing using 24-2 Humphrey standard automated perimetry, peripapillary retinal nerve fiber layer average thickness using the 3.4-mm circular scan of the Heidelberg OCT spectralis and pattern electroretinogram using CSO RetiMax device in accordance with the International Society for Clinical Electrophysiology of Vision guidelines. Results: We had three main groups: normal, glaucoma suspect, and primary open-angle glaucoma patients, and the last group included three subgroups: mild, moderate, and severe. There was significant difference in the visual field mean deviation, peripapillary retinal nerve fiber layer average thickness, and most pattern electroretinogram measured parameters between the three main groups and in between primary open-angle glaucoma subgroups. There was significant positive correlation between visual field mean deviation and the peripapillary retinal nerve fiber layer average thickness, P50 amplitude, and P50–N95 amplitude (p < 0.001, p = 0.018, and p < 0.001, respectively). Significant negative correlation was also found between peripapillary retinal nerve fiber layer average thickness and N95 amplitude (p < 0.001). Significant positive correlation was found between retinal nerve fiber layer average thickness and P50–N95 amplitude (p = 0.001). Significant negative correlation was found between peripapillary retinal nerve fiber layer average thickness and N95 amplitude (p = 0.001) and significant positive correlation of retinal nerve fiber layer average thickness with P50–N95 amplitude (p = 0.017) in primary open-angle glaucoma patients. Conclusion: Peripapillary retinal nerve fiber layer average thickness shows significant negative correlation with pattern electroretinogram N95 amplitude and a significant positive correlation with P50–N95 amplitude. In combination with optical coherence tomography, pattern electroretinogram can be used to objectively assess functional loss in glaucoma.

Sign in / Sign up

Export Citation Format

Share Document