scholarly journals Rapid and Sensitive Detection of Lung Cancer Biomarker Using Nanoporous Biosensor Based on Localized Surface Plasmon Resonance Coupled with Interferometry

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Jae-Sung Lee ◽  
Sae-Wan Kim ◽  
Eun-Yoon Jang ◽  
Byoung-Ho Kang ◽  
Sang-Won Lee ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Limit Of Detection ◽  
Surrounding Medium ◽  
Electrochemical Anodization ◽  
Localized Surface Plasmon ◽  
Label Free Detection

We propose a nanobiosensor to evaluate a lung cancer-specific biomarker. The nanobiosensor is based on an anodic aluminum oxide (AAO) chip and functions on the principles of localized surface plasmon resonance (LSPR) and interferometry. The pore-depth of the fabricated nanoporous AAO chip was 1 µm and was obtained using a two-step electrochemical anodization process. The sensor chip is sensitive to the refractive index (RI) changes of the surrounding medium and also provides simple and label-free detection when specific antibodies are immobilized on the gold-deposited surface of the AAO chip. In order to confirm the effectiveness of the sensor, the antibodies were immobilized on the surface of the AAO chip, and the lung cancer-specific biomarker was applied atop of the immobilized-antibody layer using the self-assembled monolayer method. The nanoporous AAO chip was used as a sensor system to detect serum amyloid A1, which is a lung cancer-specific biomarker. The specific reaction of the antigen-antibody contributes to the change in the RI. This in turn causes a shift in the resonance spectrum in the refractive interference pattern. The limit of detection (LOD) was found to be 100 ag/mL and the biosensor had high sensitivity over a wide concentration range.

Analysis of Localized Surface Plasmon Resonance in Ag/ITO/CdS/SiO2 Multilayered Nanostructured Composite

NANO ◽  
2015 ◽  
Vol 10 (08) ◽  
pp. 1550117
Author(s):  
Chao Liu ◽  
Jingwei Lv ◽  
Famei Wang ◽  
Qiang Liu ◽  
Haiwei Mu ◽  
...  
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Surrounding Medium ◽  
Blue Shift ◽  
Red Shift ◽  
Localized Surface Plasmon ◽  
Extinction Spectra

Multilayered nanoshells have attracted much attention due to their unique optical, electronic and magnetic properties. In this work, numerical calculation using discrete dipole approximation (DDA) is conducted to investigate the quad-layered metal nanoshell consisting of a particle with a dielectric silica (SiO2) core, inner cadium sulfide (CdS) shell, middle indium tin oxide (ITO) shell and outer metal silver (Ag) shell. The phenomenon is interpreted by plasmon hybridization theory and the Ag–ITO–CdS–SiO2 multilayered nanoshells are studied by extinction spectra of localized surface plasmon resonance. The variation in the spectrum peak with nanoparticle thickness and refractive index of the surrounding medium is derived. The electric field enhancement contour around the nanoparticles under illumination is analyzed at the plasmon resonance wavelength. The [Formula: see text], [Formula: see text], and [Formula: see text] modes red-shift with the refractive index of the surrounding medium and increase in the layer thickness causes either blue-shift or red-shift as shown by the extinction spectra. The mechanism of the red-shift or blue-shift is discussed. The [Formula: see text] mode blue-shifts and furthermore, the [Formula: see text] and [Formula: see text] modes of the Ag coated multilayered nanostructure are noticeable by comparing the extinction efficiency spectra of the Au–ITO–CdS–SiO2 and Ag–ITO–CdS–SiO2 multilayered nanoshells.

Localized Surface Plasmon Resonance Biosensor Using Ag Nanostructured Films Fabricated by a Reduction Method

2006 ◽  
Vol 915 ◽  
Author(s):  
Tomofumi Arai ◽  
Penmetcha K. R. Kumar ◽  
Koichi Awazu ◽  
Junji Tominaga
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Surrounding Medium ◽  
Optical Biosensor ◽  
Localized Surface Plasmon ◽  
Nanostructured Film ◽  
Nanostructured Films ◽  
Binding Event

AbstractIn this paper, an optical biosensor based on the localized surface plasmon resonance (LSPR) of Ag nanostructured films is proposed and demonstrated. The Ag nanostructured films, which are fabricated by the reduction of AgOx thin films, exhibit a strong LSPR at wavelengths around 370 nm in an air environment. The reflectance spectra of the Ag nanostructured film represent that the shift in the LSPR wavelength follows a linear dependence on the refractive index of the surrounding medium. By varying the concentration of streptavidin solution, we demonstrate that the Ag nanostructured films functionalized with thiol and biotin molecules can sensitively detect a binding event between biotin and streptavidin molecules.

scholarly journals Label-Free Detection of Tobramycin in Serum by Transmission-Localized Surface Plasmon Resonance

2015 ◽  
Vol 87 (10) ◽  
pp. 5278-5285 ◽  
Author(s):  
Giulia Cappi ◽  
Fabio M. Spiga ◽  
Yessica Moncada ◽  
Anna Ferretti ◽  
Michael Beyeler ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Label Free Detection

scholarly journals Label-Free Direct Detection of Saxitoxin Based on a Localized Surface Plasmon Resonance Aptasensor

Toxins ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 274 ◽  
Author(s):  
Su-Ji Ha ◽  
Jin-Ho Park ◽  
Bobin Lee ◽  
Min-Gon Kim
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Limit Of Detection ◽  
Direct Detection ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Health Food ◽  
Detection Range

Seafood is an emerging health food, and interest in improving the quality of seafood is increasing. Saxitoxin (STX) is a neurotoxin produced by marine dinoflagellates that is accumulated in seafood. It can block the neuronal transmission between nerves and muscle cell membranes, resulting in the disturbance of neuromuscular transmission and subsequent voluntary muscle paralysis. Here, we developed a new aptamer for the detection of STX using graphene oxide–systematic evolution of ligands by exponential enrichment (GO-SELEX). Furthermore, we confirmed sensitivity and selectivity of the developed aptamer specific to STX using a localized surface plasmon resonance (LSPR) sensor. The sensing chip was fabricated by fixing the new STX aptamer immobilized on the gold nanorod (GNR) substrate. The STX LSPR aptasensor showed a broad, linear detection range from 5 to 10,000 μg/L, with a limit of detection (LOD) of 2.46 μg/L (3σ). Moreover, it was suitable for the detection of STX (10, 100, and 2000 μg/L) in spiked mussel samples and showed a good recovery rate (96.13–116.05%). The results demonstrated that the new STX aptamer-modified GNR chip was sufficiently sensitive and selective to detect STX and can be applied to real samples as well. This LSPR aptasensor is a simple, label-free, cost-effective sensing system with a wide detectable range.

Label-Free Detection of Digoxin Using Localized Surface Plasmon Resonance-Based Nanobiosensor

Plasmonics ◽  
2016 ◽  
Vol 12 (1) ◽  
pp. 157-164 ◽  
Author(s):  
Alireza Nikfarjam ◽  
Ali Hossein Rezayan ◽  
Ghasem Mohammadkhani ◽  
Javad Mohammadnejad
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Label Free Detection
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