scholarly journals Mass Spectrometry-Based Proteomic Study Makes High-Density Lipoprotein a Biomarker for Atherosclerotic Vascular Disease

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Chiz-Tzung Chang ◽  
Chao-Yuh Yang ◽  
Fuu-Jen Tsai ◽  
Shih-Yi Lin ◽  
Chao-Jung Chen
Keyword(s):  
Mass Spectrometry ◽  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Atherosclerotic Vascular Disease ◽  
Future Directions ◽  
Recent Developments ◽  
Proteomic Study ◽  
Hdl Dysfunction ◽  
Apoprotein Composition

High-density lipoprotein (HDL) is a lipid and protein complex that consists of apolipoproteins and lower level HDL-associated enzymes. HDL dysfunction is a factor in atherosclerosis and decreases patient survival. Mass spectrometry- (MS-) based proteomics provides a high throughput approach for analyzing the composition and modifications of complex HDL proteins in diseases. HDL can be separated according to size, surface charge, electronegativity, or apoprotein composition. MS-based proteomics on subfractionated HDL then allows investigation of lipoprotein roles in diseases. Herein, we review recent developments in MS-based quantitative proteomic techniques, HDL proteomics and lipoprotein modifications in diseases, and HDL subfractionation studies. We also discuss future directions and perspectives in MS-based proteomics on HDL.

scholarly journals High-density lipoprotein subpopulations as substrates for the transfer of cholesteryl esters to very-low-density lipoproteins

1990 ◽  
Vol 270 (2) ◽  
pp. 441-449 ◽  
Author(s):  
M A Lasunción ◽  
A Iglesias ◽  
N Skottová ◽  
E Orozco ◽  
E Herrera
Keyword(s):  
High Density Lipoprotein ◽  
Low Density Lipoprotein ◽  
Specific Radioactivity ◽  
Density Lipoprotein ◽  
Cholesteryl Oleate ◽  
High Density ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Apoprotein Composition

1. Human total HDL (high-density lipoprotein), HDL2 and HDL3 were labelled in vitro by incubation with lipoprotein-deficient serum (LPDS) which contained either [3H]cholesteryl oleate or [14C]cholesterol under different conditions. The lipoproteins were then subfractionated by heparin-Sepharose column chromatography, and three subfractions (A, B and C) were successively eluted from each preparation of HDL, HDL2 and HDL3. When the labelling was done at 37 degrees C for 17 h, the subfractions were homogeneously labelled with [3H]cholesteryl oleate. However, when it was performed for only 30 min at 4 degrees C, the subfractions showed marked differences in the 3H specific radioactivity, which was much higher in the C fractions than in the others. 2. 3H-labelled HDL2 and HDL3 subfractions behaved differently under the precipitant action of heparin-Mn2+; fraction C (the richest in apolipoprotein E) produced the largest amount of radioactive and chemical precipitate. More 3H radioactivity, but not the cholesterol, was precipitated from HDL2 or HDL3 by the reagent, demonstrating that 3H-labelled HDL2 and HDL3 behave like their fraction C, which becomes labelled to the highest specific radioactivity despite having the smallest mass. 3. The incubation of 3H-labelled HDL subfractions with human LPDS and very-low-density lipoprotein (VLDL) at 37 degrees C increased the quantity of 3H radioactivity that was precipitated, in proportion to the amount of VLDL present in the media. These changes were attributable to the action of cholesterol ester transfer protein, since they did not occur at 4 degrees C or when human LPDS was replaced with rat LPDS. 4. Kinetics of the transfer of HDL [3H]cholesteryl oleate to VLDL showed a greater apparent Vmax for fractions A than for fractions B from either HDL2 or HDL3, whereas the apparent Km values were very similar, which suggest that this transfer process is influenced by the apoprotein composition of the donor lipoprotein.

scholarly journals SELDI-TOF mass spectrometry of High-Density Lipoprotein

2007 ◽  
Vol 5 (1) ◽  
pp. 15 ◽  
Author(s):  
Johannes HM Levels ◽  
Boris Bleijlevens ◽  
Farhad Rezaee ◽  
Johannes MFG Aerts ◽  
Joost CM Meijers
Keyword(s):  
Mass Spectrometry ◽  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density
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