scholarly journals Enhanced Production and Characterization of a Solvent Stable Amylase from Solvent TolerantBacillus tequilensisRG-01: Thermostable and Surfactant Resistant

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Soni Tiwari ◽  
Neha Shukla ◽  
Pooja Mishra ◽  
Rajeeva Gaur
Keyword(s):  
Tween 80 ◽  
Inhibitory Effect ◽  
Industrial Applications ◽  
Bacterial Strains ◽  
Enhanced Production ◽  
Bacillus Tequilensis ◽  
Amylase Production ◽  
Tween 40 ◽  
Tween 60 ◽  
Triton X

Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified asBacillus tequilensisfrom MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca2+ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity. This isolate (Bacillus tequilensisRG-01) may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

scholarly journals Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains

2010 ◽  
Vol 53 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Arlem Nascimento de Oliveira ◽  
Luiz Antonio de Oliveira ◽  
Jerusa Souza Andrade
Keyword(s):  
Amylase Activity ◽  
Tween 80 ◽  
Inhibitory Effect ◽  
Partial Characterization ◽  
Exponential Growth Phase ◽  
Enzyme Preparations ◽  
Amylase Production ◽  
Ph Range ◽  
Triton X

Amylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-mid- exponential growth phase; both were active over a pH range from 4.5 to 8.5 and temperature from 30 to 50 ºC. None of the ions studied (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ and Zn2+) was required for the catalytic activity of strain R-926; amylase activity of strain R-991 was stimulated in the presence of K+, Hg2+ and Zn2+. The surfactants SDS, Triton X-100 and Tween-80 did not have a pronounced inhibitory effect on enzyme activities; SDS and Tween-80 caused the highest stimulatory effects. Amylase activities from the rhizobia strains were reduced by up to 30% in the presence of EDTA; amylase activity of R-926 was also inhibited by HgCl2, suggesting that Ca2+and cysteine residues could be important for activity of this strain.

scholarly journals Molecular Identification of a Newly IsolatedBacillus subtilisBI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Biplab Kumar Dash ◽  
M. Mizanur Rahman ◽  
Palash Kumar Sarker
Keyword(s):  
Inorganic Nitrogen ◽  
Low Cost ◽  
Nitrogen Sources ◽  
Tween 80 ◽  
Shake Flask Culture ◽  
Lauryl Sulfate ◽  
Enhanced Production ◽  
Amylase Production ◽  
Extracellular Amylase ◽  
Production Conditions

A study was carried out with a newly isolated bacterial strain yielding extracellular amylase. The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members ofBacillussp. and identified asBacillus subtilisBI19. The effect of various fermentation conditions on amylase production through shake-flask culture was investigated. Rice flour (1.25%) as a cheap natural carbon source was found to induce amylase production mostly. A combination of peptone and tryptone as organic and ammonium sulfate as inorganic nitrogen sources gave highest yield. Maximum production was obtained after 24 h of incubation at 37°C with an initial medium pH 8.0. Addition of surfactants like Tween 80 (0.25 g/L) and sodium lauryl sulfate (0.2 g/L) resulted in 28% and 15% increase in enzyme production, respectively. Amylase production was 3.06 times higher when optimized production conditions were used. Optimum reaction temperature and pH for crude amylase activity were 50°C and 6.0, respectively. The crude enzyme showed activity and stability over a fair range of temperature and pH. These results suggest thatB. subtilisBI19 could be exploited for production of amylase at relatively low cost and time.

scholarly journals Production of cellulase by Mucor ramanniacus using submerged fermentation and its applications in biodegradation of agro-industrial waste

2021 ◽  
Author(s):  
Taiwo Dorcas Ibukunoluwa ◽  
Ademakinwa Adedeji Nelson ◽  
Zainab Adenike Ayinla ◽  
Femi Kayode Agboola
Keyword(s):  
Specific Activity ◽  
Protein A ◽  
Nitrogen Sources ◽  
Tween 80 ◽  
Growth Conditions ◽  
Cellulase Production ◽  
Industrial Applications ◽  
Inoculum Size ◽  
Industrial Wastes ◽  
Triton X

Abstract This study was undertaken to isolate and identify a novel cellulase-producing strain from a waste site (7°28’11’’N 4°31’24’’E), optimise the growth conditions, partially purify and biochemically characterise the enzyme. The potentials of the purified cellulase to hydrolyse the lignocellulosic component of some agro-industrial wastes (e.g. orange peels etc.) was also investigated. The best cellulase-producing fungus was identified as Mucor ramanniacus and the optimum conditions for cellulase production were pH (4.5), inoculum size (12 mm), carbon and nitrogen sources were carboxymethyl cellulose and sodium nitrate respectively resulting in a specific activity of 1423 Units/mg protein. A purification fold of 1.56 and 45.37 % yield were obtained after purification. The optimum pH and temperature were at 9.0 and 40°C respectively. The kinetic parameters were 0.63 ± 0.495 mg/ml, 20.21 ± 11.28 U/ml, 1001.4s− 1 for Km and Vmax and kcat respectively. Na+, K+, Ca+, Cysteine, β-mercaptoethanol and SDS were activators while Tween 80, Triton X-100 EDTA, Hg2+ and Ba2+ inhibited the enzyme. M. ramanniacus cellulase hydrolysed all agro-industrial wastes used. The partially purified M. ramanniacus cellulase showed great potential in biodegradation of various lignocellulosic substrates and the biochemical characteristics exhibited makes it suitable in industrial applications.

Effect Of Surfactant Chain Length On Emulsification Dynamics Of Self Emulsifying Formulation Of Poorly Soluble Drug

2021 ◽  
Vol 18 ◽  
Author(s):  
Shailendra Chouhan ◽  
Lalit Singh Chauhan
Keyword(s):  
Chain Length ◽  
Tween 80 ◽  
Tween 20 ◽  
Almond Oil ◽  
Peg 400 ◽  
Globule Size ◽  
Poorly Soluble ◽  
Tween 40 ◽  
Poorly Soluble Drug ◽  
Tween 60

Aim: In this work the aim was to study the chain length of surfactant on the self emulsifying system of a poorly soluble drug, aceclofenac. The selection of almond oil as a lipid vehicle was done on basis of solubility and compatibility of the vehicle with the drug. Methods: The effect of varying chain length of different surfactants of Tween series namely Tween 20, Tween 40, Tween 60 and Tween 80 was evaluated on self emulsifying efficiency by constructing pseudoternary diagrams. PEG-400 was used as co-surfactant in a definite ratio with all the surfactants to minimize their concentration. The best self emulsifying ability was exhibited by Tween 80: PEG-400 combination followed by Tween 60: PEG-400, Tween 40: PEG-400, Tween 20: PEG-400. This observation indicates that as the chain length of Tweens increases their ability to form a good microemulsion increases if same co-surfactant is used. Results: However it has also been found that the presence of unsaturated bond in Tween 80 provides it an elasticity which supports good intermixing of oil and water and leading to formation of a fine microemulsion. Six different formulations were prepared using combination of almond oil, Tween 80, PEG-400 and the drug aceclofenac. Conclusion : The formulations were subjected to various evaluation parameters such as dispersibility, transmittance, pH, globule size, polydispersibility, zeta potential, viscosity, refractive index and in vitro dissolution. The best formulation was found to have globule size of less than 100 nm, zeta potential of -3.35 ± 0.60 mV which indicates formation of a microemulsion of aceclofenac with good stability.

scholarly journals Purification and Properties of the Raw Starch Degrading α-amylase of Mutant strain: Bacillus cereus 1306

2004 ◽  
Vol 2 (2) ◽  
pp. 46-54
Author(s):  
Manoj Trivedi ◽  
S. K. Mandal
Keyword(s):  
Bacillus Cereus ◽  
Incubation Temperature ◽  
Gel Filtration ◽  
Tween 80 ◽  
Maximum Activity ◽  
Raw Starch ◽  
Purification And Properties ◽  
Amylase Production ◽  
Ph Range ◽  
Triton X

The raw starch-degrading α-amylase Produced by Bacillus cereus 1306 was purified to homogeneity by acetone precipitation and gel filtration chromatography.The Molecular weight of α-amylase was estimated to be 58KDa. The enzyme displayed maximum activity 85 Units/ml at pH 7.0 and an incubation temperature of 37˚C and Stable in the pH range of 5.0-9.0. Activity was inhibited in the presence of Hg2+,Cu2+,Fe3+ but no inhibition was observed in the presence of Zn2+. Medium containing CaCl2.2H2O enhanced amylase production over that on Ca2+-deficient medium. The detergent Tween-80 and Triton X-100 increased Biomass but Significantly Suppressed amylase production. The enzyme released large amount of glucose and maltose on hydrolysis of starch.

Orthogonal Test Design for Optimization of Emulsifying Effectiveness of Phytosterol Adding to Milk Using Tween as Emulsifiers

2013 ◽  
Vol 700 ◽  
pp. 280-283
Author(s):  
Guo Wei Shu ◽  
Ji Li Cao ◽  
He Chen ◽  
Yuan Tan ◽  
Ling Ma
Keyword(s):  
Orthogonal Experiment ◽  
Tween 80 ◽  
Orthogonal Test ◽  
Tween 20 ◽  
Test Design ◽  
Single Factor ◽  
Orthogonal Test Design ◽  
Tween 40 ◽  
Tween 60

Effect of four emulsifiers including Tween 20, Tween 40, Tween 60 and Tween 80 on emulsifying effectiveness of phytosterol adding to milk was studied by single factor and orthogonal experiment. The concentrations of four emulsifiers were all 0.2%, 0.3%, 0.4%, 0.5%, 0.6% and 0.7%. The optimal emulsified conditions showed as follows: the optimal concentrations of Tween 20, Tween 40, Tween 60 and Tween 80 were 0.40%, 0.40%, 0.20% and 0.30%, respectively, the emulsification R reached 1.043 under the conditions mentioned above.

scholarly journals Conductometric study of sodium dodecyl sulfate - nonionic surfactant (Triton X-100, Tween 20, Tween 60, Tween 80 or Tween 85) mixed micelles in aqueous solution

2012 ◽  
Vol 66 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Dejan Cirin ◽  
Mihalj Posa ◽  
Veljko Krstonosic ◽  
Maja Milanovic
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Synergistic Effect ◽  
Nonionic Surfactants ◽  
Mixed Micelles ◽  
Sodium Dodecyl ◽  
Tween 80 ◽  
Tween 20 ◽  
Dodecyl Sulfate ◽  
Tween 60 ◽  
Triton X

The present study is concerned with the determination of the critical micelle concentration (cmc) of mixed micelles of sodium dodecyl sulfate with one of five nonionic surfactants (Triton X-100, Tween 20, Tween 60, Tween 80 or Tween 85) from conductance measurements. Based on the calculated values of the ? parameters we have noticed that SDS-nonionic surfactants mostly showed strong synergistic effect. It was found that nonionic surfactants with mainly longer and more hydrophobic tail show stronger interactions with hydrophobic part of SDS, thus expressing stronger synergism. In SDS-Tween 80 binary system the strongest synergistic effect was noticed. SDS-Tween 85 micellar system showed antagonistic effect, most probably because the presence of the double bond in its three hydrophobic tails (three C18 tails) makes it sterically rigid.

Preliminary in Vitro Investigations on The Inhibitory Activity of The Original Dietary Supplement Oxidal® on Pathogenic Bacterial Strains

2020 ◽  
Vol 11 ◽  
pp. 37-43
Author(s):  
Prof. Teodora P. Popova ◽  
Toshka Petrova ◽  
Ignat Ignatov ◽  
Stoil Karadzhov
Keyword(s):  
Dietary Supplement ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Clinical Effectiveness ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Microbial Strains

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

scholarly journals Enhanced Production of Photosynthetic Pigments and Various Metabolites and Lipids in the Cyanobacteria Synechocystis sp. PCC 7338 Culture in the Presence of Exogenous Glucose

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 214
Author(s):  
YuJin Noh ◽  
Hwanhui Lee ◽  
Myeongsun Kim ◽  
Seong-Joo Hong ◽  
Hookeun Lee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Growth ◽  
Photosynthetic Pigments ◽  
Industrial Applications ◽  
Gas Chromatography Mass Spectrometry ◽  
Enhanced Production ◽  
Exogenous Glucose ◽  
Nanoelectrospray Ionization ◽  
Lipid Species ◽  
Synechocystis Sp

Synechocystis strains are cyanobacteria that can produce useful biomaterials for biofuel and pharmaceutical resources. In this study, the effects of exogenous glucose (5-mM) on cell growth, photosynthetic pigments, metabolites, and lipids in Synechocystis sp. PCC 7338 (referred to as Synechocystis 7338) were investigated. Exogenous glucose increased cell growth on days 9 and 18. The highest production (mg/L) of chlorophyll a (34.66), phycocyanin (84.94), allophycocyanin (34.28), and phycoerythrin (6.90) was observed on day 18 in Synechocystis 7338 culture under 5-mM glucose. Alterations in metabolic and lipidomic profiles under 5-mM glucose were investigated using gas chromatography-mass spectrometry (MS) and nanoelectrospray ionization-MS. The highest production (relative intensity/L) of aspartic acid, glutamic acid, glycerol-3-phosphate, linolenic acid, monogalactosyldiacylglycerol (MGDG) 16:0/18:1, MGDG 16:0/20:2, MGDG 18:1/18:2, neophytadiene, oleic acid, phosphatidylglycerol (PG) 16:0/16:0, and PG 16:0/17:2 was achieved on day 9. The highest production of pyroglutamic acid and sucrose was observed on day 18. We suggest that the addition of exogenous glucose to Synechocystis 7338 culture could be an efficient strategy for improving growth of cells and production of photosynthetic pigments, metabolites, and intact lipid species for industrial applications.

scholarly journals Synthesis and Evaluation of Biological Activity of New Arylphosphoramidates

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Amal Thebti ◽  
M. A. K. Sanhoury ◽  
H-I. Ouzari ◽  
T. Barhoumi-slimi
Keyword(s):  
Biological Activity ◽  
Ir Spectroscopy ◽  
Acetylcholinesterase Activity ◽  
Inhibitory Effect ◽  
Bacterial Strains ◽  
Significant Inhibitory Effect ◽  
Subsequent Addition

The synthesis of new substituted arylphosphoramidates is performed in two steps through phosphorylation of the corresponding alcohols followed by aminolysis. The formation of the desired phosphoramidates depends on the subsequent addition of the two alcohols with the amine being added at the last step. The products were obtained in 58–95% yields. They were characterized mainly by multinuclear (1H, 13C, 31P, and 19F) NMR and IR spectroscopy. In addition, the antimicrobial and antiacetylcholinesterase activities were evaluated. The results showed acetylcholinesterase activity by some compounds, whilst no significant inhibitory effect against the tested bacterial strains has been recorded.

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