scholarly journals Quantification of Caffeoylquinic Acids in Coffee Brews by HPLC-DAD

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Marzieh Moeenfard ◽  
Lígia Rocha ◽  
Arminda Alves
Keyword(s):  
Total Content ◽  
Caffeoylquinic Acids ◽  
Caffeoylquinic Acid ◽  
Major Isomer ◽  
Potential Sources ◽  
Coffee Brews

The influence of different brewing conditions on the concentration of the main caffeoylquinic acids (3-caffeoylquinic acid (3-CQA), 4-caffeoylquinic acid (4-CQA), and 5-caffeoylquinic acid (5-CQA)) was investigated. For this purpose, twenty-four coffee brews were extracted and analyzed using HPLC-DAD at 325 nm. Our findings demonstrate the great impact of brewing techniques on the caffeoylquinic acids (CQAs) content. The major isomer was 3-CQA, accounting for about 50% of the total CQAs, followed by 5-CQA and 4-CQA, accounting for about 24–36% for each one. The total content of CQAs was in the range of 45.79 to 1662.01 mg/L, found in iced cappuccino and pod espresso, respectively. In conclusion, this study demonstrates that coffee brews, in particular those prepared using pressurized methods, can be considered as the potential sources of antioxidants such as CQAs.

scholarly journals Quantitative and Qualitative Identification of Phytochemical Constituents of Sida Rhombifolia Leaves Extract

2019 ◽  
Vol 8 (2S4) ◽  
pp. 403-408
Keyword(s):  
Quantitative Analysis ◽  
Cardiac Glycosides ◽  
Total Content ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Biological Compounds ◽  
Potential Sources ◽  
Pharmaceutical Industries ◽  
Phytochemical Constituents ◽  
Sida Rhombifolia

Anti-inflammatory, analgesic, antimicrobial, anti-arthritic, antibacterial, antispasmodic, hypoglycaemic and hepatoprotective characteristics of Sida rhombifolia are included in the Malvaceae family. Most of the plant belongs to the Malvaceae family, as they comprise a number of phytochemicals and biological compounds, are potential sources of different medications. This is study on Sida rhombifolia leaf extract's tophytochemical constituen ts. Phytochemical screening results in herbal stan-dardizat ion and preparation and may relate the components to thei r medicinal / pharmacological uses. The qualitative phytochemical analysis has shown that the extract is positive for saponins, flavonoids, alkaloids, phenols and same extract is negative for carbohydrate, tannins, glycosides, cardiac glycosides, terpenoids, coumarins, steroid& phytosteroids, phlobutanins, anthraquinones. Quantitative analysis of phytochemicals includes the estimation of flavonoid, tannin and total content of phenol. The result suggest that the Sida rhombifolia leaves extract consist plenty of phytochemicals beneficial in alternative medical and pharmaceutical industries.

An Efficient Strategy Based on Liquid–Liquid Extraction With Acid Condition and HSCCC for Rapid Enrichment and Preparative Separation of Three Caffeoylquinic Acid Isomers From Mulberry Leaves

2019 ◽  
Vol 57 (8) ◽  
pp. 738-744 ◽  
Author(s):  
Li-Tao Wang ◽  
Ming-Zhu Gao ◽  
Qing Yang ◽  
Qi Cui ◽  
Yue Jian ◽  
...  
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Macroporous Resin ◽  
Acid Condition ◽  
Two Phase ◽  
Caffeoylquinic Acids ◽  
Mulberry Leaves ◽  
Caffeoylquinic Acid ◽  
Liquid Liquid Extraction ◽  
Separation Strategy

Abstract Morus alba L. is a medicinal plant that contains a high amount of caffeoylquinic acids such as 3-caffeoylquinic acid (3-CQA), 5-caffeoylquinic acid (5-CQA) and 4-caffeoylquinic acid (4-CQA). This study aimed to establish a fast and efficient method for separating caffeoylquinic acids from mulberry leaves by using high-speed countercurrent chromatography coupled with macroporous resin. D101 resin showed better adsorption and desorption capacity for three caffeoylquinic acids among six macroporous resin adsorbents. The contents of 3-CQA, 5-CQA and 4-CQA reached for 4.77%, 18.95% and 9.84% through one cycle of D101 resin, which were 3.13-fold, 4.57-fold and 4.78-fold more than those in crude extracts, respectively. With a two-phase solvent system of ethyl acetate-water (1:1, V/V), >93% purity of target compounds were obtained in one cycle during 150 min with the recovery yields of 80.59%, 99.56% and 94.21% for 3-CQA, 5-CQA and 4-CQA, respectively. The structural identification of target compounds was carried out by ESI-MS, 1H NMR and 13C NMR spectra. The present result represented an easy and efficient separation strategy for the utilization of mulberry resource.

scholarly journals Comparison of Phenolic Compounds and the Antioxidant Activities of Fifteen Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’ in China

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 325 ◽  
Author(s):  
Jinyan Gong ◽  
Bingquan Chu ◽  
Lingxiao Gong ◽  
Zhongxiang Fang ◽  
Xiaoxu Zhang ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
High Performance Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Chrysanthemum Morifolium ◽  
Caffeoylquinic Acids ◽  
Caffeoylquinic Acid ◽  
Chrysanthemum Morifolium Ramat

This study investigated the phenolic compounds of 15 Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’, including 6 ‘Duoju’ and 9 ‘Taiju’, using high performance liquid chromatography (HPLC). The antioxidant activities of these ‘Hangbaiju’ were estimated by DPPH, ABTS and FRAP assays. Results show that a total of 14 phenolic compounds were detected in these flowers, including 3 mono-caffeoylquinic acids, 3 di-caffeoylquinic acids, 1 phenolic acid and 7 flavonoids. ‘Duoju’ and ‘Taiju’ possess different concentrations of phenolic compounds, and ‘Taiju’ exhibits higher caffeoylquinic acids and stronger antioxidant activities than ‘Duoju’. Caffeoylquinic acids show a strong correlation with the antioxidant activities of the samples. Principal component analysis (PCA) reveals an obvious separation between ‘Duoju’ and ‘Taiju’, using phenolic compounds as variables. Apigenin-7-O-glucoside, 3,5-di-O-caffeoylquinic acid, luteolin and acacetin were found to be the key phenolic compounds to differentiate ‘Duoju’ from ‘Taiju’.

scholarly journals Valeriana rigida Ruiz & Pav. Root Extract: A New Source of Caffeoylquinic Acids with Antioxidant and Aldose Reductase Inhibitory Activities

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1079
Author(s):  
Guanglei Zuo ◽  
Hyun-Yong Kim ◽  
Yanymee N. Guillen Quispe ◽  
Zhiqiang Wang ◽  
Kang-Hyuk Kim ◽  
...  
Keyword(s):  
Aldose Reductase ◽  
High Speed ◽  
High Performance ◽  
Root Extract ◽  
Zone Refining ◽  
Food Ingredient ◽  
Caffeoylquinic Acids ◽  
Caffeoylquinic Acid ◽  
Inhibitory Activities ◽  
Counter Current

Valeriana rigida Ruiz & Pav. (V. rigida) has long been used as a herbal medicine in Peru; however, its phytochemicals and pharmacology need to be scientifically explored. In this study, we combined the offline 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH)-/ultrafiltration-high-performance liquid chromatography (HPLC) and high-speed counter-current chromatography (HSCCC)/pH-zone-refining counter-current chromatography (pH-zone-refining CCC) to screen and separate the antioxidants and aldose reductase (AR) inhibitors from the 70% MeOH extract of V. rigida, which exhibited remarkable antioxidant and AR inhibitory activities. Seven compounds were initially screened as target compounds exhibiting dual antioxidant and AR inhibitory activities using DPPH-/ultrafiltration-HPLC, which guided the subsequent pH-zone-refining CCC and HSCCC separations of these target compounds, namely 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, 3,4-O-di-caffeoylquinic acid, 3,5-O-di-caffeoylquinic acid, 4,5-O-di-caffeoylquinic acid, and 3,4,5-O-tri-caffeoylquinic acid. These compounds are identified for the first time in V. rigida and exhibited remarkable antioxidant and AR inhibitory activities. The results demonstrate that the method established in this study can be used to efficiently screen and separate the antioxidants and AR inhibitors from natural products and, particularly, the root extract of V. rigida is a new source of caffeoylquinic acids with antioxidant and AR inhibitory activities, and it can be used as a potential functional food ingredient for diabetes.

scholarly journals Comparison of Phenolic Compounds and Antioxidant Activities of Fifteen Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’ in China

2019 ◽  
Author(s):  
Jinyan Gong ◽  
Bingquan Chu ◽  
Lingxiao Gong ◽  
Zhongxiang Fang ◽  
Xiaoxu Zhang ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
High Performance Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Chrysanthemum Morifolium ◽  
Caffeoylquinic Acids ◽  
Caffeoylquinic Acid ◽  
Chrysanthemum Morifolium Ramat

This study investigated the phenolic compounds of 15 Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’, including 6 ‘Duoju’ and 9 ‘Taiju’ using high performance liquid chromatography. The antioxidant activities of these ‘Hangbaiju’ were estimated by DPPH, ABTS and FPAR assays. Results showed that a total of 14 phenolic compounds were detected in these flowers, including 3 mono-caffeoylquinic acids, 3 di-caffeoylquinic acids, 1 phenolic acid and 7 flavonoids. ‘Duoju’ and ‘Taiju’ possessed different concentration of phenolic compounds, and ‘Taiju’ exhibited higher caffeoylquinic acids and stronger antioxidant activities than ‘Duoju’. Caffeoylquinic acids showed a strong correlation with the antioxidant activities of the samples. Principal component analysis revealed an obvious separation between ‘Duoju’ and ‘Taiju’ using phenolic compounds as variables. Apigenin-7-O-glucoside, 3,5-di-O-caffeoylquinic acid, luteolin and acacetin were found to be the key phenolic compounds to differentiate ‘Duoju’ from ‘Taiju’.

scholarly journals FLAVONOIDS AND PHENILPROPANOIDS OF THE ABOVE GROUND ORGANS OF THE BUPLEURUM MUL-TINERVE DC. OF THE FLORA OF THE BAIKAL REGION

2020 ◽  
pp. 121-128
Author(s):  
Vera Mikhaylovna Mirovich ◽  
Daniil Nikolayevich Olennikov ◽  
Svetlana Andreyevna Petukhova ◽  
Alina Alekseevna Posokhina
Keyword(s):  
Baikal Region ◽  
Raw Materials ◽  
Total Content ◽  
Quinic Acid ◽  
Standard Samples ◽  
Flavonoid Aglycones ◽  
Caffeoylquinic Acid ◽  
Quantitative Content ◽  
Irkutsk Region ◽  
Derivatives Of

A study of the flavonoids and phenylpropanoids of the aerial organs of Bupleurum multinerve was carried out. Samples of raw materials were collected in the Baikal region in the vicinity of. Verkholensk, Irkutsk region. The raw materials were collected during the flowering period in 2019 in the herb-grass association. For analysis, raw materials were extracted with 70% ethyl alcohol in an ultrasonic bath for 30 minutes, followed by centrifugation of the extract. Compounds were identified by the UPLC-DAD-ESI-MS method in comparison with standard samples. In the aerial organs of B. multinerve, 15 compounds were identified (8 flavonoids and 7 phenylpropanoids). Flavonoids B. multinerve are derivatives of quercetin, kempferol, isoramnetin; phenylpropanoids – esters of quinic acid with coffee, ferulic, coumaric. The contents of B. multinerve compounds were first established: quercetin-3-O-glucuronide, kempferol-3-O-glucoside (astragaline), 5-O-p-coumaroylquinic acid, 5-O-feruloylquinic acid, 3-O-feruloylquinic acid, 3,5-di-O-caffeoylquinic acid; 4,5-di-O-caffeoylquinic acid. The quantitative content of glycosides of flavonoids isoramnetin-3-O-rutinoside (narcissin) 20.74 mg/g, quercetin-3-O-rutinoside (rutin) 19.53 mg/g, quercetin-3 was determined by MK-HPLC-UV in the aerial organs of B. multinerve -O-glucoside (isocvercitrin) 2.24 mg/g, camperol-3-O-glucoside (astragaline) 0.39 mg/g, quercetin-3-O-glucuronide 0.25 mg/g; quercetin flavonoid aglycones 0.43 mg/g; isoramnetin 0.53 mg/g; the predominant phenylpropanoids were 5-O-caffeoylquinic acid 6.60 mg/g, 3,5-di-O-caffeylquinic acid 1.58 mg/g. The total content of identified flavonoids was 44.97 mg/g, phenylpropanoids 9.53 mg/g.

COMPOSITION AND CONTENT OF PHENOLIC COMPOUNDS IN DIFFERENT FRACTIONS OF EXTRACT FROM PLANTS OF ARNICA FOLIOSA NUTT.

2020 ◽  
pp. 139-147
Author(s):  
Tatiana Anatol'yevna Krol' ◽  
Lidiya Viktorovna Zinnatshina ◽  
Eugenia Ramil'yevna Gatiatulina ◽  
Andrey Ivanovich Radimich ◽  
Olga Leonidovna Saybel ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Total Content ◽  
Biologically Active ◽  
Diethyl Ester ◽  
Alternative Source ◽  
Caffeoylquinic Acid ◽  
Array Detection ◽  
Derivatives Of

Among representatives of the genus Arnica L., the Arnica montana L. species is the most studied and widely used for medical purposes. However, due to the fact that the A. montana species is endangered in most European countries, the possibility to use Arnica foliosa Nutt. as an alternative source of phytochemicals is being investigated. A deeper study of the chemical composition of A. foliosa extract may give the opportunity to expand the spectrum of its possible application. The purpose of the research was to perform a detailed study of the composition and content of biologically active compounds the aerial part of A. foliosa by high performance liquid chromatography with diode array detection in combination with high-resolution mass spectrometry. Sixteen phenolic compounds were detected in ethylacetate and butanol fractions of A. foliosa. There were identified thirteen derivatives of caffeoylquinic acid and three flavonoids. In the chloroform and diethyl ester fractions, phenolic compounds were absent. It was established that the ethylacetate fraction contains big amounts of two phenolic compounds – 3,4-dicaffeoylquinic and 4,5-dicaffeoylquinic acids, and eight compounds were presented in trace or very small quantities. All sixteen phenolic compounds were found in the butanol fraction, but their total content was almost 2-fold less than in the ethylacetate fraction.

scholarly journals HS-SPME/GC/MS profiles of convectively and microwave roasted Ivory Coast Robusta coffee brews

2011 ◽  
Vol 29 (No. 2) ◽  
pp. 151-160 ◽  
Author(s):  
G. Budryn ◽  
E. Nebesny ◽  
J. Kula ◽  
T. Majda ◽  
W. Krysiak
Keyword(s):  
Ivory Coast ◽  
Total Content ◽  
Sensory Properties ◽  
Roasted Coffee ◽  
Volatile Substances ◽  
Microwave Roasting ◽  
Robusta Coffee ◽  
Coffee Beans ◽  
Roasted Coffee Beans ◽  
Coffee Brews

Robusta coffee beans were convectively, microwave, and convectively-microwave heated at 230°C, 700 W, and 230°C/700 W (coupled heating), respectively, over periods of time ensuring the optimum sensory properties of the brews. HS-SPME/GC/MS analysis of the emissions from brews of the roasted coffee beans revealed 119 compounds. The highest total content of volatile substances was found in the brews prepared from convectively-microwave roasted coffee beans but the microwave roasting resulted in the most acceptable sensory properties of the brew aroma, presumably because of the lowest concentrations of the burnt note imparting compounds.

scholarly journals PHENYLPROPANOIDS OF PARASENECIO HASTATUS (COMPOSITAE) AND THEIR WOUND-HEALING ACTIVI-TY

2020 ◽  
pp. 97-105
Author(s):  
Daniil Nikolayevich Olennikov ◽  
Nadezhda Konstantinovna Chirikova ◽  
Arsen Vladimirovich Tsyrenzhapov
Keyword(s):  
Wound Healing ◽  
Asian Countries ◽  
Mass Flowering ◽  
Caffeoylquinic Acids ◽  
Caffeoylquinic Acid ◽  
Healing Effect ◽  
Wound Healing Effect ◽  
First Time ◽  
Maximum Content ◽  
Active Substances

Parasenecio hastatus (L.) H. Koyama (Cacalia hastata L., Compositae) is a well-known medicinal plant widespread in Siberia and Southeast Asia and used in the practice of traditional medicine in Asian countries as a wound-healing remedy. Scientific research confirmed the effectiveness of drugs containing P. hastatus, however, there is no any information about active substances identified (Ayushieva et al., 2007, 2009). In this work, it was revealed that the butanol fraction of P. hastatus leaves showed the most pronounced wound-healing effect. As a result of chromatographic separation of this fraction, 12 phenylpropanoids were identified as 5-O-caffeoylquinic acid, caffeic acid, and for the first time 4-O-caffeoylquinic, 1,5-di-O-caffeoylquinic, 3,4-di-O-caffeoylquinic, 3,5-di-O-caffeoylquinic, 4,5-di-O-caffeoylquinic, 3,4,5-tri-O-caffeoylquinic, chichoric, 3-O-feruloylquinic, 5-O-feruloylquinic acids and echinacoside. Using the microcolumn HPLC, it was established that the maximum content of phenylpropanoids in P. hastatus leaves is observed in the mass flowering period (60.83 mg/g). Compounds 5-O-caffeoylquinic acid (16.34–39.37 mg/g) and 3,5-di-O-caffeoylquinic acid (10.77–22.87 mg/g) were the dominant components of the phenolic complex as well as the main active substances with wound-healing effect. It was shown that the activity of 3,5-di-O-caffeoylquinic acid was higher than the activity of 5-O-caffeoylquinic acid. Thus, it was demonstrated that caffeoylquinic acids and the drugs containing them are promising wound-healing agents.

Inhibition of HIV Infection by Caffeoylquinic Acid Derivatives

1993 ◽  
Vol 4 (4) ◽  
pp. 235-240 ◽  
Author(s):  
N. Mahmood ◽  
P. S. Moore ◽  
N. De Tommasi ◽  
F. De Simone ◽  
S. Colman ◽  
...  
Keyword(s):  
Related Compound ◽  
Specific Binding ◽  
Selective Inhibition ◽  
Virus Infectivity ◽  
Hiv Replication ◽  
Caffeoylquinic Acids ◽  
Caffeoylquinic Acid ◽  
Guiera Senegalensis ◽  
Anti Hiv

The caffeoylquinic acids 3,4,5-tri-O-caffeoylquinic acid (1) and 4,5-di-O-caffeoylquinic acid (2), as well as caffeic acid (4) and synapoic acid (5) were isolated from the plant Securidaka longipedunculata (polygalaceae). 1 exhibited a greater selective inhibition of HIV replication than 2 which had an anti-HIV activity similar to that of 3,4,5-tri-O-galloylquinic acid (3), isolated from Guiera senegalensis (combretaceae); 4 and 5 were ineffective and the structurally related compound rosmarinic acid (6) had only slight anti-HIV activity. Studies of the actions of these compounds suggest that inhibition of the viral reverse transcriptase in vitro is non-specific and that they act by specific binding to gp120 which prevents its interaction with CD4 on T-lymphocytes and thus inactivates virus infectivity.

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