scholarly journals Biosynthesis of Silver Nanoparticles UsingAegle marmelos(Bael) Fruit Extract and Its Application to Prevent Adhesion of Bacteria: A Strategy to Control Microfouling

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
A. Nithya Deva Krupa ◽  
Vimala Raghavan
Keyword(s):  
Silver Nanoparticles ◽  
Biological Synthesis ◽  
Isolation And Identification ◽  
Fruit Extract ◽  
Force Microscopy ◽  
Atomic Force ◽  
Marine Biofilm ◽  
Marine Biofilms ◽  
Biosynthesized Agnps ◽  
Rdna Analysis

Marine biofilms formed due to adhesion of bacteria and other microorganisms on submerged surfaces are generally considered to be a major form of microfouling. Subsequent attachment of larvae of higher organisms like barnacles, mussels, and so forth, on marine biofilms, causes macrofouling. Several approaches have been used to prevent micro- and macrofouling. Silver nanoparticles (AgNPs) are known to exhibit strong inhibitory and antimicrobial activity. Biological synthesis of AgNPs is rapidly gaining importance due to its growing success. Hence, the present study is focused on the biosynthesis of AgNPs using fruit extract ofAegle marmelosand its characterization through UV-Vis spectrophotometer, X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FTIR), and atomic force microscopy (AFM). Further isolation and identification of marine biofilm forming bacteria were carried out through 16S rDNA analysis. The antimicrofouling effect of the biosynthesized AgNPs was tested against marine biofilm forming bacteria and the results suggested that it could effectively inhibit biofilm formation. This preliminary study has proved that AgNPs may be used as antimicrofouling agent for the prevention of biofouling in the early stages.

scholarly journals Green Synthesis of Silver Nanoparticles from several NTFP Plants

2016 ◽  
Vol 8 (1) ◽  
pp. 106-111
Author(s):  
Somnath BHOWMIK ◽  
Badal Kumar DATTA ◽  
Ajay Krishna SAHA ◽  
Pradyut CHAKMA ◽  
Narayan Chandra MANDAL
Keyword(s):  
Silver Nanoparticles ◽  
Plant Extract ◽  
Silver Ions ◽  
Biological Synthesis ◽  
Synthesis Of Nanoparticles ◽  
Force Microscopy ◽  
Atomic Force ◽  
One Step ◽  
Aqueous Leaf Extract ◽  
Uv Visible

The biological synthesis of nanoparticles using plant extracts plays an important role in the field of nanotechnology. In this study, rapid, simple approach was applied for synthesis of silver nanoparticles using , Clerodendrum infortunatum, Mucuna interrupta, Phlogancanthus thyrsiflorus and Sansevieria trifasciata aqueous leaf extract. The plant extract acts both as reducing agent as well as capping agent. To identify the compounds responsible for reduction of silver ions, the functional groups present in plant extract were investigated by FTIR. Various techniques used to characterize synthesized nanoparticles are Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and UV–Visible spectrophotometer. Results confirmed that this protocol was simple, rapid, one step, eco-friendly, non-toxic and might be an alternative conventional physical/chemical methods. Conversion of silver nanoparticles takes place at room temperature without the involvement of any hazardous chemicals.

Antimicrobial Activity of Silver Nanoparticles Synthesized by Streptomyces Species JF714876

2012 ◽  
Vol 5 (1) ◽  
pp. 1638-1642 ◽  
Author(s):  
Vidyasagar G M ◽  
Shankaravva B ◽  
R Begum ◽  
Imrose ◽  
Sagar R ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silver Ions ◽  
Human Beings ◽  
Streptomyces Species ◽  
Force Microscopy ◽  
E Coli ◽  
Extracellular Synthesis ◽  
Atomic Force ◽  
Uv Visible

Microorganisms like fungi, actinomycetes and bacteria are considered nanofactories and are helpful in the production of nanoparticles useful in the welfare of human beings. In the present study, we investigated the production of silver nanoparticles from Streptomyces species JF714876. Extracellular synthesis of silver nanoparticles by Streptomyces species was carried out using two different media. Silver nanoparticles were examined using UV-visible, IR and atomic force microscopy. The size of silver nanoparticles was in the range of 80-100 nm. Antimicrobial activity of silver nanoparticle against bacteria such as E. coli, S. aureus, and dermatophytes like T. rubrum and T. tonsurans was determined. Thus, this study suggests that the Streptomyces sp. JF741876 can produce silver ions that can be used as an antimicrobial substance.

scholarly journals Antibacterial Activity of Silver Nanoparticles Synthesized by Bark Extract of Syzygium cumini

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ram Prasad ◽  
Vyshnava Satyanarayana Swamy
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cost Effective ◽  
Diffusion Method ◽  
Bark Extract ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cost Effective Method ◽  
Uv Visible ◽  
Major Attention

The unique property of the silver nanoparticles having the antimicrobial activity drags the major attention towards the present nanotechnology. The environmentally nontoxic, ecofriendly, and cost-effective method that has been developed for the synthesis of silver nanoparticles using plant extracts creates the major research interest in the field of nanobiotechnology. The synthesized silver nanoparticles have been characterized by the UV-visible spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method, and it was found that the biogenic silver nanoparticles have antibacterial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Azotobacter chroococcum WR 9, and Bacillus licheniformis (MTCC 9555).

scholarly journals GREEN TECHNOLOGY MEDIATED SYNTHESIS OF SILVER NANOPARTICLES FROM MOMORDICA CHARANTIA FRUIT EXTRACT AND ITS BACTERICIDAL ACTIVITY

2017 ◽  
Vol 10 (3) ◽  
pp. 196 ◽  
Author(s):  
S C Joshi ◽  
Utkarsh Kaushik ◽  
Aproova Upadhyaya ◽  
Priyanka Sharma
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Atomic Force Microscope ◽  
Momordica Charantia ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Atomic Force ◽  
Vis Spectroscopy ◽  
Scanning Electron

ABSTRACTObjective: The synthesis of nanoparticles from biological processes is evolving a new era of research interests in nanotechnology. Silver nanoparticlesare usually synthesized by chemicals and physical method, which are quite toxic and flammable in nature. This study deals with an environmentfriendly biosynthesis process of antibacterial silver nanoparticles using Momordica charantia fruit.Methods: AgNO3 (5 mM) was allowed to react with fruit extract of M. charantia. Biosynthesis of AgNPs was optimized by changing temperature,pH, and solvent. The silver nanoparticles so formed were characterized using ultraviolet-visible (UV-VIS) spectroscopy, Fourier transform infraredspectroscopy (FTIR), dynamic light scattering (DLS), atomic force microscope (AFM), and scanning electron microscopy (SEM).Results: UV-VIS spectra show absorption peak between 420 and 430 nm. The FTIR analysis showed the alcoholic, lactam, and nitro group presentin the plant extract, which were responsible for the reduction in AgNPs. The SEM images showed the size distribution of the nanoparticles and theaverage size was found to be 50-100 nm. By DLS analysis and AFM analysis, average sizes of the silver nanoparticles were of 150 nm. The results ofthese analyses confirmed the formation of silver nanoparticles. Silver nanoparticles were tested against Bacillus cereus and Staphylococcus epidermidisstrains using disc diffusion method and were found to be effective.Conclusion: Silver nanoparticles so synthesized in this study using fruit extract of M. charantia are simple, easy, and effective technique of nanoparticlesproduction.Keywords: Silver nanoparticles, Momordica charantia, Optimization, Antibacterial, Atomic force microscope, Scanning electron microscopy.

scholarly journals Silver Nanoparticles Embedded in Natural Rubber Films: Synthesis, Characterization, and Evaluation ofIn VitroToxicity

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Caroline S. Danna ◽  
Dalita G. S. M. Cavalcante ◽  
Andressa S. Gomes ◽  
Leandra E. Kerche-Silva ◽  
Eidi Yoshihara ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Natural Rubber ◽  
Chinese Hamster ◽  
Cell Lineage ◽  
Microscopic Analysis ◽  
Visible Spectrum ◽  
Electron Microscopic ◽  
Force Microscopy ◽  
Atomic Force

Natural rubber (NR) films can reduce silver metal ions forming embedded metal nanoparticles, a process that could be described as green synthesis. The NR films acting as a reactor generate and incorporate silver nanoparticles (AgNPs). Organic acids and amino acids play a crucial role in the formation of AgNPs. The plasmon extinction obtained in the UV-visible spectrum shows the presence of nanoparticles in the film after dipping the NR film into a solution of silver nitrate at 80°C. Electron microscopic analysis confirms the presence of AgNPs in the NR film and characterization by atomic force microscopy shows a change in the roughness of the NR film with AgNPs. In addition, our preliminary results fromin vitrotoxicity studies (MTT and comet assays) of the NR films and NR films with silver nanoparticles (NR/Ag) show that they are not toxic to cell lineage CHO-K1 (cells from the ovary of a Chinese hamster), an important result for potential medical applications.

scholarly journals Hydrophilic Silver Nanoparticles Loaded into Niosomes: Physical–Chemical Characterization in View of Biological Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 1177 ◽  
Author(s):  
Federica Rinaldi ◽  
Elena del Favero ◽  
Johannes Moeller ◽  
Patrizia Nadia Hanieh ◽  
Daniele Passeri ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Human Serum ◽  
Nile Red ◽  
Chemical Characterization ◽  
Entrapment Efficiency ◽  
Tween 20 ◽  
Biological Applications ◽  
Force Microscopy ◽  
Atomic Force ◽  
New Perspective

Silver nanoparticles (AgNPs) are widely used as antibacterial agents and anticancer drugs, but often their low stability limits their mass production and broad applications. The use of niosomes as a carrier to protect and envelop AgNPs gives a new perspective to solve these problems. In this study, AgNPs were functionalized with sodium 3-mercapto-1-propanesulfonate (3MPS) to induce hydrophilic behavior, improving loading in Tween 20 and Span 20 niosomes (NioTw20 and NioSp20, respectively). Entrapment efficiency was evaluated by UV analyses and is around 1–4%. Dimensions were investigated by means of dynamic light scattering (DLS) (<2RH> = 140 ± 4 nm and <2RH> = 251 ± 1 nm respectively for NioTw20 + AgNPs and NioSp20 + AgNPs) and were compared with those by atomic force microscopy (AFM) and small angle X ray scattering (SAXS) analyses. Stability was assessed in water up to 90 days, and both in bovine serum and human serum for up to 8 h. In order to characterize the local structure of niosomes, SAXS measurements have been performed on Tween 20 and Span 20 empty niosomes and loaded with AgNPs. The release profiles of hydrophilic probe calcein and lipophilic probe Nile Red were performed in HEPES buffer and in human serum. All these features contribute to conclude that the two systems, NioTw20 + AgNPs and NioSp20 + AgNPs, are suitable and promising in the field of biological applications.

Properties of Silver Nanoparticles Prepared by the Electric Spark Dispersion Method

2013 ◽  
Vol 872 ◽  
pp. 74-78 ◽  
Author(s):  
S.P. Zhuravkov ◽  
Evgeny Plotnikov ◽  
Dmitry Martemiyanov ◽  
Nikolay A. Yavorovsky ◽  
Ulrich Hasse ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Silver Nanoparticles ◽  
Structural Characteristics ◽  
Silver Particles ◽  
Dispersion Method ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
Light Scattering Spectroscopy

The morphological and structural characteristics of nanoscale silver particles obtained by the method of electric spark dispersion of metal granules in the liquid aprotic medium were obtained using atomic force microscopy, transmission electron microscopy, and dynamic light scattering spectroscopy. The specific surface, morphology, structure and the distribution by size of the particles are presented.

scholarly journals Preparation of Chitosan-Silver Nanoparticles in Nonaqueous Medium under Heating

2015 ◽  
Vol 58 ◽  
pp. 1-8
Author(s):  
A.B.M. Nazmul Islam
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Sodium Dodecyl ◽  
Homogeneous System ◽  
Nonaqueous Medium ◽  
Silver Particles ◽  
Force Microscopy ◽  
Atomic Force

Chitosan-silver nanoparticles are prepared in nonaqueous medium. In this work, sodium dodecyl sulfate (SDS) was introduced into the dimethylformamide (DMF) solution during silver reduction from solution of its precursor salt AgNO3, acting as a stabilizing agent to prevent aggregation of silver nanoparticles, while chitosan is used as the solid support to embedded silver particles therein, resulting in chitosan-silver (CS-Ag) nanoparticle as suspension in the medium. The reaction started as homogeneous system which turned into heterogeneous with the formation of particles. The properties of CS-Ag nanoparticles are studied under two different salt concentrations and characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-Vis). Wide particle size distribution of synthesized nanoparticles depicts that concentration of AgNO3, which is responsible for the morphology, stability and particle size distribution, should be optimized, suggesting a lower salt concentration is favorable.

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