scholarly journals Synthesis and Characterization of Silver and Gold Nanoparticles Using Aqueous Extract of Seaweed,Turbinaria conoides,and Their Antimicrofouling Activity

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Sri Ramkumar Vijayan ◽  
Prakash Santhiyagu ◽  
Muthukkumarasamy Singamuthu ◽  
Natarajan Kumari Ahila ◽  
Ravindran Jayaraman ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Aqueous Extract ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Antibiofilm Activity ◽  
Ag Nps ◽  
Au Nps ◽  
Silver And Gold Nanoparticles ◽  
Marine Biofilm ◽  
Turbinaria Conoides

Silver and gold nanoparticles were synthesized using an aqueous extract of the seaweedTurbinaria conoidesand their antibiofilm activity against marine biofilm forming bacteria is reported here. The UV-Vis spectra showed the characteristics SPR absorption band for Ag NPs at 421 and for Au NPs at 538 nm. Further, the synthesized nanoparticles were characterized using FT-IR, XRD, FESEM, EDX, and HRTEM analysis. Spherical and triangular nanostructures of the Ag and Au nanoparticles were observed between the size ranges of 2–17 nm and 2–19 nm, respectively. The synthesized Ag NPs are efficient in controlling the bacterial biofilm formation; however, Au NPs did not show any remarkable antibiofilm activity. The maximum zone of inhibition was recorded againstE. coli(17.6±0.42 mm), followed bySalmonellasp.,S. liquefaciens, andA. hydrophila. The macrotube dilution method inferred the MIC (20–40 µL mL−1) and MBC (40–60 µL mL−1) of Ag NPs. The CLSM images clearly showed the weak adherence and disintegrating biofilm formation of marine biofilm bacterial strains treated with Ag NPs. TheArtemiacytotoxicity assay recorded the LC50value of88.914±5.04 µL mL−1. Thus the present study proved the efficiency of Ag NPs as a potent antimicrofouling agent and became the future perspective for the possible usage in the biofouling related issues in the aquaculture installations and other marine systems.

Preparation and Characterization of Silver and Gold Nanoparticles and Study Influence on Physical Properties of PVA/PVP nanocomposites

2017 ◽  
Vol 13 (1) ◽  
pp. 4628-4639
Author(s):  
A. M. Abdelghany ◽  
Mahrous. S. Meikhail ◽  
A. A. ALdhabi
Keyword(s):  
Gold Nanoparticles ◽  
Spherical Shape ◽  
X Ray Diffraction ◽  
Ag Nps ◽  
Au Nps ◽  
Silver And Gold Nanoparticles ◽  
Plant Extraction ◽  
Chenopodium Murale ◽  
Transmission Electron

Gold nanoparticles (Au NPs) and Silver nanoparticles (Ag NPs) ware prepared by “green” synthesis extraction using the Chenopodium murale leaf extract, the obtained (Ag NPs and Au NPs) were investigated by UV/Vis. absorption spectroscopy, transmission electron microscopy, Zetasizer, XRD. The plant extraction leads to produce nanoparticles of spherical shape with size range from 4 to 22 nm. Polyvinyl alcohol (PVA) and polyvinyl Pyrolidone (PVP) blend with mixed silver and gold nanoparticles were prepared by casting method. Amorphous feather of doping polymers blend was characterized by X-ray diffraction. Significant changes within the polymer matrix were monitored from infrared spectroscopy which indicates the interaction between polymer blend and mixed nanoparticles. Both indirect and direct optical energy gaps are calculated and discussed.

scholarly journals The Effect of Liquid Rubber Addition on the Physicochemical Properties, Cytotoxicity and Ability to Inhibit Biofilm Formation of Dental Composites

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1704
Author(s):  
Krzysztof Pałka ◽  
Małgorzata Miazga-Karska ◽  
Joanna Pawłat ◽  
Joanna Kleczewska ◽  
Agata Przekora
Keyword(s):  
Cell Viability ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Type Material ◽  
Dental Composites ◽  
Antibiofilm Activity ◽  
Ceramic Filler ◽  
Liquid Rubber ◽  
Flow Type ◽  
Type Composite

The aim of this study was to evaluate the effect of modification with liquid rubber on the adhesion to tooth tissues (enamel, dentin), wettability and ability to inhibit bacterial biofilm formation of resin-based dental composites. Two commercial composites (Flow-Art–flow type with 60% ceramic filler and Boston–packable type with 78% ceramic filler; both from Arkona Laboratorium Farmakologii Stomatologicznej, Nasutów, Poland) were modified by addition of 5% by weight (of resin) of a liquid methacrylate-terminated polybutadiene. Results showed that modification of the flow type composite significantly (p < 0.05) increased the shear bond strength values by 17% for enamel and by 33% for dentine. Addition of liquid rubber significantly (p < 0.05) reduced also hydrophilicity of the dental materials since the water contact angle was increased from 81–83° to 87–89°. Interestingly, modified packable type material showed improved antibiofilm activity against Steptococcus mutans and Streptococcus sanguinis (quantitative assay with crystal violet), but also cytotoxicity against eukaryotic cells since cell viability was reduced to 37% as proven in a direct-contact WST-8 test. Introduction of the same modification to the flow type material significantly improved its antibiofilm properties (biofilm reduction by approximately 6% compared to the unmodified material, p < 0.05) without cytotoxic effects against human fibroblasts (cell viability near 100%). Thus, modified flow type composite may be considered as a candidate to be used as restorative material since it exhibits both nontoxicity and antibiofilm properties.

scholarly journals The Antibiofilm Activity of Dual-Function Tail Tubular Protein B from KP32 Phage

2018 ◽  
Author(s):  
Ewa Brzozowska ◽  
Anna Pyra ◽  
Krzysztof Pawlik ◽  
Sabina Górska ◽  
Andrzej Gamian
Keyword(s):  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Hydrolytic Activity ◽  
Bacterial Biofilm ◽  
Dual Function ◽  
Antibiofilm Activity ◽  
Synergistic Activity ◽  
Antibiotic Action ◽  
Phage Protein

Background: Dual function tail tubular proteins (TTP) belonging to the lytic bacteriophages are the interesting group of biologically active enzymes. Surprisingly, apart from their structural function, they are also polysaccharide hydrolyzes destroying bacterial extracellular components. One of the representatives of this group is TTPB from Klebsiella pneumoniae phage &ndash; KP32. TTPB hydrolyzes exopolysaccharide (EPS) of Klebsiella pneumoniae and Enterococcus faecalis strain. This depolymerizing feature was associated with the activity to prevent bacterial biofilm formation. TTPB can inhibit biofilm formation by K. pneumoniae, Enterobacter cloacae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa strains. Moreover, synergistic activity with antibiotic action has been observed, most likely due to depolymerases&rsquo; facilitation of contact of antibiotic with bacterial cells. Methods: TTPB was overexpressed in E coli system, purified and tested towards the bacterial strains using agar overlay method. The hydrolytic activity of TTPB was performed using EPSs of K. pneumoniae PCM2713 and E. cloacae ATCC 13047 as the substrates. Next, we determined the reducing sugar (RS) levels in the TTPB/EPS mixtures, regarding the RS amount obtained after acidic hydrolysis. The antibiofilm activity of TTPB has been set down on bacterial biofilm using a biochemical method. Finally, we have demonstrated the synergistic activity of TTPB with kanamycin. Results: For the first time, the hydrolytic activity of TTPB towards bacterial EPSs has been shown. TTPB releases about a half of the whole RS amount of EPSs belonging to K. pneumoniae PCM 2713 and E. cloacae ATCC 13047 strains. 1.12 &micro;M of the phage protein reduces biofilm of both strains by over 60%. Destroying the bacterial biofilm the phage protein improves the antibiotic action increasing kanamycin effectiveness up to four times.

Preparation of Au nanoparticles by Anthemis xylopoda flowers aqueous extract and their application for alkyne/aldehyde/amine A3-type coupling reactions

RSC Advances ◽  
2015 ◽  
Vol 5 (57) ◽  
pp. 46240-46246 ◽  
Author(s):  
Mahmoud Nasrollahzadeh ◽  
S. Mohammad Sajadi
Keyword(s):  
Gold Nanoparticles ◽  
Green Synthesis ◽  
Aqueous Extract ◽  
Au Nanoparticles ◽  
Coupling Reactions ◽  
Au Nps ◽  
Type Coupling

This paper reports on the green synthesis of gold nanoparticles (Au NPs) by Anthemis xylopoda flowers aqueous extract without the addition of surfactant agents and their application as stable catalysts for the synthesis of propargylamines.

scholarly journals Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 442 ◽  
Author(s):  
Fabio Fontecha-Umaña ◽  
Abel Guillermo Ríos-Castillo ◽  
Carolina Ripolles-Avila ◽  
José Juan Rodríguez-Jerez
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Biofilm Formation ◽  
Antimicrobial Properties ◽  
Bacterial Biofilm ◽  
Antimicrobial Efficacy ◽  
Ag Nps ◽  
Zno Nps ◽  
E Coli ◽  
Food Contact Surfaces

Food contact surfaces are primary sources of bacterial contamination in food industry processes. With the objective of preventing bacterial adhesion and biofilm formation on surfaces, this study evaluated the antimicrobial activity of silver (Ag-NPs) and zinc oxide (ZnO-NPs) nanoparticle-containing polyester surfaces (concentration range from 400 ppm to 850 ppm) using two kinds of bacteria, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli), and the prevention of bacterial biofilm formation using the pathogen Listeria monocytogenes. The results of antimicrobial efficacy (reductions ≥ 2 log CFU/cm2) showed that at a concentration of 850 ppm, ZnO-NPs were effective against only E. coli (2.07 log CFU/cm2). However, a concentration of 400 ppm of Ag-NPs was effective against E. coli (4.90 log CFU/cm2) and S. aureus (3.84 log CFU/cm2). Furthermore, a combined concentration of 850 ppm Ag-NPs and 400 ppm ZnO-NPs showed high antimicrobial efficacy against E. coli (5.80 log CFU/cm2) and S. aureus (4.11 log CFU/cm2). The results also showed a high correlation between concentration levels and the bacterial activity of Ag–ZnO-NPs (R2 = 0.97 for S. aureus, and R2 = 0.99 for E. coli). They also showed that unlike individual action, the joint action of Ag-NPs and ZnO-NPs has high antimicrobial efficacy for both types of microorganisms. Moreover, Ag-NPs prevent the biofilm formation of L. monocytogenes in humid conditions of growth at concentrations of 500 ppm. Additional studies under different conditions are needed to test the durability of nanoparticle containing polyester surfaces with antimicrobial properties to optimize their use.

Antibiofilm activity and cytotoxicity of silk sericin against Streptococcus mutans bacteria in biofilm: an in vitro study

2020 ◽  
Vol 29 (Sup4) ◽  
pp. S25-S35
Author(s):  
Pornanong Aramwit ◽  
Supamas Napavichayanum ◽  
Prompong Pienpinijtham ◽  
Yousef Rasmi ◽  
Nipaporn Bang
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
In Vitro Study ◽  
Bacterial Biofilm ◽  
Vitro Study ◽  
Antibiofilm Activity ◽  
Diphenyltetrazolium Bromide ◽  
Infrared Spectrometer ◽  
Disruption Effect

Objective: To investigate the potential of sericin extracted by different methods to inhibit biofilm formation (prevention) and disrupt already formed biofilm (treatment). Method: In this in vitro study, sericin was extracted by heat, acid, alkali and urea. Streptococcus mutans bacteria were cultivated in the presence of various concentrations of sericin to evaluate antibiofilm formation using cell density assay (inhibition effect before biofilm formed). Conversely, various concentrations of sericin were added to a biofilm already formed by Streptococcus mutans bacteria, and the viability of bacteria assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (disruption effects after biofilm formed). Structures of extracted sericin were evaluated using circular dichroism and Fourier-transform infrared spectrometer. Results: The urea-extracted sericin at all concentrations (12.5mg/ml, 25mg/ml, 50mg/ml and 100mg/ml) showed the highest potential antibiofilm activity in terms of both inhibition and disruption effects, compared with sericin extracted by heat, acid or alkali. The heat-extracted and acid-extracted sericin were found to reduce the biofilm formation dose-dependently, while the alkali-extracted sericin did not show either inhibition or disruption effect on the bacterial biofilm. The urea-extracted sericin also killed the bacteria residing within the biofilm, possibly due to its modified structure which may destabilise the bacterial cell wall, leading to membrane disintegration and, finally, cell death. Conclusion: Our results demostrated the antibiofilm activity of sericin. This could form the basis of further research on the mechanism and application of sericin as a novel antibiofilm agent.

scholarly journals Derivatives of the Mouse Cathelicidin-Related Antimicrobial Peptide (CRAMP) Inhibit Fungal and Bacterial Biofilm Formation

2014 ◽  
Vol 58 (9) ◽  
pp. 5395-5404 ◽  
Author(s):  
Katrijn De Brucker ◽  
Nicolas Delattin ◽  
Stijn Robijns ◽  
Hans Steenackers ◽  
Natalie Verstraeten ◽  
...  
Keyword(s):  
Amino Acid ◽  
Antimicrobial Peptide ◽  
Inhibitory Activity ◽  
Biofilm Formation ◽  
Specific Activity ◽  
Cell Types ◽  
Bacterial Biofilm ◽  
Antibiofilm Activity ◽  
Content Type ◽  
Derivatives Of

ABSTRACTWe identified a 26-amino-acid truncated form of the 34-amino-acid cathelicidin-related antimicrobial peptide (CRAMP) in the islets of Langerhans of the murine pancreas. This peptide, P318, shares 67% identity with the LL-37 human antimicrobial peptide. As LL-37 displays antimicrobial and antibiofilm activity, we tested antifungal and antibiofilm activity of P318 against the fungal pathogenCandida albicans. P318 shows biofilm-specific activity as it inhibitsC. albicansbiofilm formation at 0.15 μM without affecting planktonic survival at that concentration. Next, we tested theC. albicansbiofilm-inhibitory activity of a series of truncated and alanine-substituted derivatives of P318. Based on the biofilm-inhibitory activity of these derivatives and the length of the peptides, we decided to synthesize the shortened alanine-substituted peptide at position 10 (AS10; KLKKIAQKIKNFFQKLVP). AS10 inhibitedC. albicansbiofilm formation at 0.22 μM and acted synergistically with amphotericin B and caspofungin against mature biofilms. AS10 also inhibited biofilm formation of different bacteria as well as of fungi and bacteria in a mixed biofilm. In addition, AS10 does not affect the viability or functionality of different cell types involved in osseointegration of an implant, pointing to the potential of AS10 for further development as a lead peptide to coat implants.

scholarly journals Small-Molecule Compound SYG-180-2-2 to Effectively Prevent the Biofilm Formation of Methicillin-Resistant Staphylococcus aureus

2022 ◽  
Vol 12 ◽  
Author(s):  
Lulin Rao ◽  
Yaoguang Sheng ◽  
Jiao Zhang ◽  
Yanlei Xu ◽  
Jingyi Yu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Small Molecule ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Alveolar Epithelial Cells ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Antibiofilm Activity ◽  
Methicillin Resistant ◽  
Small Molecule Compound

The resistance of methicillin-resistant Staphylococcus aureus (MRSA) has augmented due to the abuse of antibiotics, bringing about difficulties in the treatment of infection especially with the formation of biofilm. Thus, it is essential to develop antimicrobials. Here we synthesized a novel small-molecule compound, which we termed SYG-180-2-2 (C21H16N2OSe), that had antibiofilm activity. The aim of this study was to demonstrate the antibiofilm effect of SYG-180-2-2 against clinical MRSA isolates at a subinhibitory concentration (4 μg/ml). In this study, it was showed that significant suppression in biofilm formation occurred with SYG-180-2-2 treatment, the inhibition ranged between 65.0 and 85.2%. Subsequently, confocal laser scanning microscopy and a bacterial biofilm metabolism activity assay further demonstrated that SYG-180-2-2 could suppress biofilm. Additionally, SYG-180-2-2 reduced bacterial adhesion and polysaccharide intercellular adhesin (PIA) production. It was found that the expression of icaA and other biofilm-related genes were downregulated as evaluated by RT-qPCR. At the same time, icaR and codY were upregulated when biofilms were treated with SYG-180-2-2. Based on the above results, we speculate that SYG-180-2-2 inhibits the formation of biofilm by affecting cell adhesion and the expression of genes related to PIA production. Above all, SYG-180-2-2 had no toxic effects on human normal alveolar epithelial cells BEAS-2B. Collectively, the small-molecule compound SYG-180-2-2 is a safe and effective antibacterial agent for inhibiting MRSA biofilm.

Green Synthesis of Metal Nanoparticles from Adiantum Frond: Comparative Analysis on Cancer Cell Lines

2020 ◽  
Vol 10 (6) ◽  
pp. 806-816
Author(s):  
Sabiha Zamani ◽  
Danish Idrees ◽  
Babita Jha ◽  
Anal K. Jha
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Cancer Cell Lines ◽  
Ag Nps ◽  
Au Nps

Background:: At the present time, silver and gold nanoparticles are emerging as promising agents for cancer therapy. The anticancer activities of these nanoparticles have been evaluated against numerous human cancer cell lines. Still, few reports were existing against the breast cancer cell lines and most of these studies have mainly used chemically mediated nanoparticles. Objective:: This study reports the in vitro anticancer activity of bioinspired Nanoparticles like, silver and gold against MCF-7 cells (breast cancer cell line) as well as HEK293 cell (human embryonic kidney cell line) by MTT assay. Methods: These metal nanoparticles, Ag-NPs and Au-NPs were biologically synthesized using Adiantum sp. aqueous leaves extract. The Adiantum sp. can reduce HAuCl4 solution to Au-NPs and AgNO3 to Ag-NPs within 30 mins. The formation of NPs was confirmed by characterization techniques such as UV-vis spectrophotometer, SEM and XRD studies. Additionally, the anticancer activity was analysed by cell viability (3(4,5-dimethly-thiazol-2-yl)- 2,5-diphenyl tetrazo-lium bromide assay). Results: The synthesized nanoparticles from Adiantum sp. were characterized by UV-visible spectroscopy. The role of functional groups was analysed using a Fourier Transform Infrared (FTIR) spectrophotometer. The XRD pattern clearly exemplified that the nanoparticles formed in this present synthesis are crystalline in nature. Furthermore, in MTT assay study, both NPs have shown cytotoxicity at different concentrations ranging from 2.5 to 100 μg/ml. The data reveal that the NPs from Adiantum explicitly, inhibits the viability of cancerous cell only and are non-cytotoxic to HEK293 cells in the tested concentration range. Conclusion: The results provide a preliminary guidance that Adiantum mediated silver and gold nanoparticles might be used to treat breast cancer; however, it necessitates clinical studies to ascertain their potential as anticancer agents.

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