scholarly journals Clinical and Structural Features of Sperm Head Vacuoles in Men Included in the In Vitro Fertilization Programme

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Nina Fekonja ◽  
Jasna Štrus ◽  
Magda Tušek Žnidarič ◽  
Katja Knez ◽  
Eda Vrtacnik Bokal ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Human Sperm ◽  
Structural Features ◽  
Fertilization Rate ◽  
Sperm Head ◽  
Vitro Fertilization

The human sperm head vacuoles and their role in male infertility are still poorly understood. The aim of this study was to identify the clinical and ultrastructural features of human sperm head vacuoles in men included in the in vitro fertilization programme: men with normal (normozoospermia) and impaired sperm morphology (teratozoospermia). The sperm samples were observed under 6000-time magnification using motile sperm organelle morphology examination (MSOME). The proportion of sperm with head vacuoles was evaluated and related to the outcome of in vitro fertilization. The sperm of men with impaired sperm morphology was characterized by a higher proportion of sperm head vacuoles. The sperm head vacuoles were related to impaired semen quality (sperm concentration, motility, and morphology) but were not influenced by male factors (semen volume, height, age, weight, or body mass index). Moreover, sperm head vacuoles were related to impaired fertilization rate merely after classical in vitro fertilization (IVF), while there was no relation to pregnancy. In a subgroup of men, the sperm was fixed and observed by transmission electron microscopy (TEM). The ultrastructural study revealed that sperm head vacuoles are large nuclear indentations of various sizes and positions, packed with membranous material organized in membrane whorls (MW).

Human sperm morphology and in vitro fertilization: sperm tail defects are prognostic for fertilization failure

Andrologia ◽  
2009 ◽  
Vol 30 (1) ◽  
pp. 43-47 ◽  
Author(s):  
C. C. Lim ◽  
S. E. M. Lewis ◽  
M. Kennedy ◽  
E. T. Donnelly ◽  
W. Thompson
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Morphology ◽  
Human Sperm ◽  
Fertilization Failure ◽  
Vitro Fertilization ◽  
Sperm Tail

scholarly journals Re-evaluation of the value of sperm morphology in classical in vitro fertilization in a Northeastern Chinese population

2019 ◽  
Vol 47 (9) ◽  
pp. 4134-4142 ◽  
Author(s):  
Dong-liang Zhu ◽  
Hong-guo Zhang ◽  
Rui-xue Wang ◽  
Yu-ting Jiang ◽  
Rui-zhi Liu
Keyword(s):  
In Vitro Fertilization ◽  
Chinese Population ◽  
Sperm Morphology ◽  
Semen Analysis ◽  
Fertilization Rate ◽  
Vitro Fertilization ◽  
Group 2 ◽  
Normal Fertilization ◽  
Group 1

Objective This study aimed to re-evaluate the clinical value of a 4% cut-off threshold of sperm morphology in in vitro fertilization (IVF) in a cohort of a Northeastern Chinese population. Methods A total of 375 IVF cycles that met strict inclusion criteria were included. These cycles were conducted with semen analysis and oocyte fertilization. A total of 188 embryo-transferred cycles proceeded. According to sperm morphology, 375 cycles were divided into group 1 (329 cycles, <4% normal sperm morphology rate [NSMR]) and group 2 (46 cycles, ≥4% NSMR), and 188 transferred cycles into group A (151 cycles, < 4% NSMR) and group B (37 cycles, ≥4% NSMR). Results The fertilization and normal fertilization rates were significantly lower in group 1 than in group 2. The normal fertilization rate was significantly correlated with an NSMR < 4% or ≥4%, but the fertilization rate was not significantly correlated with the NSMR. No significant differences were found in pregnancy outcomes between groups A and B. Conclusions This study suggests that infertile patients with an NSMR < 4% are more likely to have a poor normal fertilization status in IVF.

Comparing Two Density Methods of Semen Preparation in Human Ejaculates

2021 ◽  
pp. 938-942
Author(s):  
Ram Dayal ◽  
Sumer Singh ◽  
Prabhat Kumar ◽  
Kamla Singh
Keyword(s):  
In Vitro Fertilization ◽  
Density Gradient ◽  
Semen Quality ◽  
Intrauterine Insemination ◽  
Sperm Concentration ◽  
Test Tube ◽  
Human Beings ◽  
Vitro Fertilization ◽  
Density Methods

Test tube baby is always a fascinating field of human reproductive biological science playing an important role in treating infertility. Poor semen quality is the major cause of infertility in human beings. Preparation of semen samples for intrauterine insemination (IUI) or in-vitro fertilization (IVF) is a key point in the success of test-tube embryo production. The study compared two different methods of semen preparation viz. double density gradient (DDG) and single density gradient (SDG) in patients seeking IUI / IVF treatment with their consent and permission of the hospital. Semen ejaculate from patients (n=100) was divided equally into two equal volume parts. One half of each sample was treated with DDG and another half with the SDG method of semen preparation. Results showed that sperm concentration was significantly higher (P>0.05) in SDG compared to DDG treated samples which were 58.65±181 and 49.89±180 Million/ml, respectively. Sperm motility of type-a and type-b both were significantly higher (P>0.05) in SDG compared to DDG treated samples which were 91.85± 3.15 and 68.85 ± 26.15. It is concluded that the single density gradient method is better than the double density gradient for semen ejaculates preparation during the treatment of male infertility using the in-vitro fertilization technique.

scholarly journals Prosaposin in seminal plasma on the day of oocyte retrieval is associated with normal fertilization and embryo development in in vitro fertilization cycles

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e8177
Author(s):  
Chun Xu ◽  
Jiali Cai ◽  
Lanlan Liu ◽  
Jianzhi Ren
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Sperm Concentration ◽  
Oocyte Retrieval ◽  
Estimating Equation ◽  
Fertilization Rate ◽  
Semen Parameters ◽  
Vitro Fertilization ◽  
Normal Fertilization

The prospective study including 166 participants aims to evaluate the association between seminal prosaposin and the outcomes of in vitro fertilization (IVF) cycles in humans. The generalized linear model (GLM) was used to analyze the associations between seminal prosaposin concentrations and normal fertilization rates and good embryos proportion. The generalized estimating equation (GEE) was used to evaluate the association between embryo parameters and the prosaposin concentrations. Each model was adjusted for age of the couples, female basal FSH, AFC and BMI, starting dose and oocyte yield of IVF cycles and smoker. GLM models suggested that prosaposin was significantly associated with fertilization rate (P = 0.005) and good embryo proportion (P = 0.038) while none of the semen parameters (sperm concentration, motility, progressive motility, normal morphology rate, postwash sperm concentration and motility) was significantly associated with the parameters in the cohort. Using GEE, it was also shown that prosaposin was positively associated with the occurrence of early cleavage and negatively associated with uneven cleavage pattern on day 3. In both the overall population and the normozoospermia patients, the prosaposin was significantly associated with pregnancy with adjustment with covariates. In conclusion, our data suggested that seminal prosaposin concentration could provide more information regarding normal fertilization and embryo development in IVF than traditional semen parameters.

Differential influence of recombinant non-glycosylated and glycosylated glycodelin on human sperm function: comparative studies with hamster spermatozoa

2001 ◽  
Vol 13 (3) ◽  
pp. 111 ◽  
Author(s):  
B. Dutta ◽  
R. Ain ◽  
P. B. Seshagiri ◽  
A. A. Karande
Keyword(s):  
In Vitro Fertilization ◽  
Acrosome Reaction ◽  
Human Sperm ◽  
Fertilization Rate ◽  
Sperm Function ◽  
Vitro Fertilization ◽  
Placental Protein 14 ◽  
Placental Protein

Glycodelin, also known as placental protein 14, has been implicated in endometriosis-related infertility. To determine the role of glycodelin and its glycosylated state, the influence of recombinant nonglycosylated-glycodelin (nongly-glycodelin) and glycosylated-glycodelin (gly-glycodelin) on human sperm function was evaluated. Whereas there was a significant (P<0.001) increase in the capacitation of nongly-glycodelin-treated spermatozoa compared with untreated controls (28.8 1.0% v. 21 1.5% respectively), treatment of spermatozoa with gly-glycodelin markedly (P<0.001) inhibited capacitation (10.7 0. 3%); acrosome reaction (AR) remained unaltered in all treatments. In a zona-free hamster egg penetration assay, the egg penetration index was higher (P<0.001) with nongly-glycodelin-treated spermatozoa (3.4 0.3) than with gly-glycodelin-treated spermatozoa (0.4 0.1) and untreated spermatozoa (1.6 0.2). A similar influence of glycodelin on capacitation was observed with hamster spermatozoa. However, the AR rate was higher (P<0. 01) in nongly-glycodelin-treated spermatozoa (39.4 1.6%) than in either gly-glycodelin-treated spermatozoa (19.3 2.0%) or untreated controls (30.0 1.2%). Moreover, the in vitro fertilization rate was significantly (P<0.01) higher with nongly-glycodelin treated-spermatozoa compared with untreated spermatozoa (77.5 2.3% v. 52.9 4.3%) and gly-glycodelin-treated spermatozoa (38.3 6.5%; P<0. 05). These results indicate that whereas nongly-glycodelin improves, gly-glycodelin inhibits, capacitation and fertilization potential of human and hamster spermatozoa, and that the glycosylation status of glycodelin determines its influence on sperm function.

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