scholarly journals L-Asparaginase Activity of Fungal Endophytes from Tabernaemontana heyneana Wall. (Apocynaceae), Endemic to the Western Ghats (India)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Chandramouli Manasa ◽  
Monnanda Somaiah Nalini

“Endophytes,” the microbes residing within the plant tissues, are important sources of secondary metabolites. Tabernaemontana heyneana Wall., a medicinal tree, endemic to the Western Ghats with rich ethnobotanical history and unique chemical diversity, was selected to study fungal endophytes and evaluate them for L-asparaginase activity. Healthy plant parts were selected for the isolation of endophytes following standard isolation protocols. A total of 727 isolates belonging to 20 taxa were obtained. The isolates comprised of bark (11%), twig (22%), leaf (43%), fruit (12.0%), and seeds (12%). Endophytes such as Colletotrichum, Curvularia, Fusarium, Phomopsis, Verticillium, and Volutella colonized T. heyneana plant parts. Fusarium sp., Phomopsis spp., isolate Thlf01, and Fusarium solani were the dominant genera of bark, twig, leaf, fruits, and seed samples, respectively. The endophytes were screened for their ability to utilize L-asparagine by plate assay method. Fusarium spp. exhibited a high level of activity among the nine endophytes tested positive for L-asparaginase activity. Studies underline the potentials of endophyte-derived fungal L-asparaginases as sources of chemotherapeutic agents.

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Monnanda Somaiah Nalini ◽  
Ningaraju Sunayana ◽  
Harischandra Sripathy Prakash

Endophytes constitute an important component of microbial diversity, and in the present investigation, seven plant species with rich ethnobotanical uses representing six families were analyzed for the presence of endophytic fungi from their natural habitats during monsoon (May/June) and winter (November/December) seasons of 2007. Fungal endophytes were isolated from healthy plant parts such as stem, root, rhizome, and inflorescence employing standard isolation methods. One thousand five hundred and twenty-nine fungal isolates were obtained from 5200 fragments. Stem fragments harbored more endophytes (80.37%) than roots (19.22%). 31 fungal taxa comprised of coelomycetes (65%), hyphomycetes (32%), and ascomycetes (3%). Fusarium, Acremonium, Colletotrichum, Chaetomium, Myrothecium, Phomopsis, and Pestalotiopsis spp. were commonly isolated. Diversity indices differed significantly between the seasons (P<0.001). Species richness was greater for monsoon isolations than winter. Host specificity was observed for few fungal endophytes. UPGMA cluster analysis grouped the endophytes into distinct clusters on the basis of genetic distance. This study is the first report on the diversity and host-specificity of endophytic fungal taxa were from the semi evergreen forest type in Talacauvery subcluster of Western Ghats.


2020 ◽  
Vol 12 (11) ◽  
pp. 16502-16509
Author(s):  
Sandeep Das ◽  
K.P. Rajkumar ◽  
K.A. Sreejith ◽  
M. Royaltata ◽  
P.S. Easa

Abstract: The Resplendent Shrub Frog, Raorchestes resplendens Biju, Shouche, Dubois, Dutta, & Bossuyt, 2010 is a Critically Endangered species endemic to the Western Ghats and was considered to be restricted to a three-square kilometer patch atop Anamudi summit.  In this study, we report 36 new locations of the species from the Anamalai massif of the southern Western Ghats.  Niche-based prediction modelling suggests that the species is restricted to Anamalai massif.  The call description of this frog is also provided for the first time. The preferred microhabitat of the frog is Chrysopogon grass clumps in the marshy/swampy montane grassland ecosystem. Restricted to a small area with controlled burning management practiced in its habitat, R. resplendens needs immediate attention.


Author(s):  
Jayesh Anerao ◽  
Vikas Jha ◽  
Nishaat Shaikh ◽  
Apurva Shivalkar ◽  
Aishwarya Nityanand ◽  
...  

Author(s):  
Yevhen Maltsev ◽  
Elena Kezlya ◽  
Svetlana Maltseva ◽  
Balasubramanian Karthick ◽  
Petr Dvořák ◽  
...  

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