scholarly journals Classification of Epidemic Community-Acquired Methicillin-ResistantStaphylococcus aureusby Anatomical Site of Isolation

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Jill C. Roberts
Keyword(s):  
Infectious Disease ◽  
Staphylococcus Aureus ◽  
Invasive Disease ◽  
Anatomical Site ◽  
Wound Infections ◽  
Nasal Swabs ◽  
The Usa ◽  
Clinically Significant ◽  
Serious Disease

Methicillin-resistantStaphylococcus aureuscontributes significantly to cost, morbidity, and mortality due to infectious disease. We surveyed community-associated MRSA isolates to determine which strains were present within anatomical sites of interest. The most likely sources of MRSA among anatomic sites swabbed were wounds followed by the nasal cavity. The USA 300 MRSA strain was most commonly isolated among wound infections while nasal swabs largely yielded USA 100 MRSA. The frequency of isolation of USA 100 amongst community-associated strains is clinically significant as this strain is often correlated with invasive disease, exhibits broad antibiotic resistance, and has been considered to be hospital associated. The potential of USA 100 to cause serious disease and the frequency of its isolation suggest an important reservoir for opportunistic infection. These data demonstrate that MRSA epidemic clones are widespread among the community.

scholarly journals Prevalence of Multiple Antibiotic Resistant Infections in Diabetic versus Nondiabetic Wounds

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Urvish Trivedi ◽  
Shamini Parameswaran ◽  
Andrew Armstrong ◽  
Diana Burgueno-Vega ◽  
John Griswold ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Staphylococcus Aureus ◽  
Multidrug Resistant ◽  
Diabetic Patients ◽  
Wound Infections ◽  
Antibiotic Resistant ◽  
Foot Ulceration ◽  
Multidrug Resistant Organisms ◽  
Initial Hypothesis ◽  
The Usa

Diabetes mellitus (DM) affects 23.6 million people in the USA and approximately 20–25% of diabetic patients will develop foot ulceration during the course of their disease. Up to a quarter of these patients will develop infections that will necessitate amputation. Although many studies report that the rates of antibiotic resistant infections have increased dramatically in the DM population over the last decade, to our knowledge there have been no reports directly comparing the rates of antibiotic resistant infections in DM versus non-DM wounds. We performed a retrospective study comparing the wound infections of 41 DM patients to those of 74 non-DM patients to test the hypothesis that infections with multidrug resistant organisms (MDRO) were more prevalent in the DM population. We found that 63.4% of DM and 50% of non-DM patients had MDRO infections, which was not statistically different. However, 61% of the DM patients hadPseudomonasinfections compared to only 18.9% of non-DM patients. Furthermore, DM patients had significantly more coinfections with bothPseudomonasandStaphylococcus aureus. Though our initial hypothesis was incorrect, we demonstrated a significant correlation betweenPseudomonasandPseudomonas/S. aureuscoinfections within DM wounds.

An Investigation of the Carriage Rate of Methicillin-Resistant Staphylococcus aureus in Pigs in the Western Province of Vietnam

2012 ◽  
Vol 12 ◽  
pp. 91-98
Author(s):  
Thi Diem Thi Vo ◽  
An Doan Mai Huynh ◽  
Hoa Thi Ngo ◽  
Duong Thuy Nguyen ◽  
Thi Minh Tam Tran
Keyword(s):  
Staphylococcus Aureus ◽  
Pcr Assay ◽  
Carriage Rate ◽  
Methicillin Resistant ◽  
Western Province ◽  
The Us ◽  
Nasal Swabs ◽  
The Usa ◽  
Infection Source ◽  
Mrsa Strains

Methicillin-resistant Staphylococcus aureus (MRSA) transmission between pigs and humans can be a dangerous infection source for the community. To control and prevent such disease, MRSA research in healthy pigs has been conducted in the US and Holland. This paper reports the first such study in Vietnam. This study aims to optimize the method for investigating MRSA carriage in tonsil and nasal swabs of healthy pigs. Potential samples were selected rapidly by Multiplex PCR (M-PCR) assay with 3 primers (Staph 756, mecA, Sa442) and then combined with conventional methods to detect and pick up MRSA strains. These strains were grouped based on genome pattern by pulsed field gel electrophoresis (PFGE). This study showed that we successfully isolated MRSA and the rate of MRSA carriage samples was 3.63%, much lower than that reported in the USA (49%) and Holland (39%), however the USA and Holland studies investigated pigs from industrial scale piggeries, whereas our study investigated pigs from small farms, which is the usual approach to pig farming in Vietnam. The MRSA patterns identified and associated MRSA strains were different from identified MRSA strains in other countries. This research provides important information about the carriage rate of MRSA in pigs in the Western province of Vietnam and demonstrates the importance of ongoing investigations in this area.

Surveillance for severe community-associated methicillin-resistant Staphylococcus aureus infection

2009 ◽  
Vol 137 (12) ◽  
pp. 1674-1678 ◽  
Author(s):  
P. WIERSMA ◽  
M. TOBIN D'ANGELO ◽  
W. R. DALEY ◽  
J. TUTTLE ◽  
K. E. ARNOLD ◽  
...  
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Case Fatality ◽  
Invasive Disease ◽  
Methicillin Resistant ◽  
Risk Of Death ◽  
Clinical Syndromes ◽  
The Usa ◽  
Severe Infections ◽  
Traditional Risk Factors

SUMMARYCommunity-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has rapidly emerged in the USA as a cause of severe infections in previously healthy persons without traditional risk factors. We describe the epidemiology of severe CA-MRSA disease in the state of Georgia, USA and analyse the risk of death associated with three different clinical syndromes of CA-MRSA disease – pneumonia, invasive disease, and skin and soft-tissue infections (SSTIs). A total of 1670 cases of severe CA-MRSA disease were reported during 2005–2007. The case-fatality rate was 3·4%; sex and race of fatal and non-fatal cases did not differ significantly. While CA-MRSA pneumonia and invasive disease were less common than SSTIs, they were about 15 times more likely to result in death [risk ratio 16·69, 95% confidence interval (CI) 10·28–27·07 and 13·98, 95% CI 7·74–25·27, respectively]. When controlling for age and the presence of other clinical syndromes the odds of death in patients manifesting specific severe CA-MRSA syndromes was highest in those with pneumonia (odds ratio 11·34). Possible risk factors for severe CA-MRSA SSTI and pneumonia included the draining of lesions without medical assistance and an antecedent influenza-like illness.

scholarly journals Clinical and microbiological characteristics of communityacquired methicillin-resistant Staphylococcus aureus pneumonia

2012 ◽  
Vol 2 (1) ◽  
pp. 7 ◽  
Author(s):  
Eric Martin ◽  
Jane Colmer-Hamood ◽  
Wesam Frandah ◽  
Rishi Raj ◽  
Kenneth Nugent
Keyword(s):  
Staphylococcus Aureus ◽  
Subcutaneous Tissue ◽  
Empiric Therapy ◽  
Invasive Disease ◽  
Beta Lactam ◽  
Methicillin Resistant ◽  
Pubmed Database ◽  
Organ System Failure ◽  
The Usa ◽  
Staphylococcus Aureus Pneumonia

Methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) infections now occur in healthy adults in community settings. We searched the PubMed database to identify relevant articles on the clinical presentation, epidemiology, virulence, and treatment of community-acquired MRSA (CA-MRSA) infections, including pneumonia. This information was summarized in a narrative review.MRSA infections cause approximately 30 infections per 100,000 people per year in the USA, and twenty percent of these infections are secondary to CA-MRSA. These community-acquired infections often involve the skin and subcutaneous tissue but can also involve visceral tissues such as the lung and bone. The overall mortality in patients with invasive disease is approximately 10%; it approaches 50% in patients with pneumonia. The bacterial isolates from these infections have the staphylococcal chromosome cassette <em>mec</em> types 4 and 5. This genetic characteristic produces beta-lactam resistance and helps distinguish these isolates from hospital- acquired MRSA, which usually have <em>mec</em> types 1-3. Some CA-MRSA isolates release the Panton-Valentine leukocidin (PVL), which causes neutropenia and tissue necrosis; other toxins also contribute to the virulence of these infections. Empiric therapy should include vancomycin or linezolid. CA-MRSA infections can have fulminant courses and high mortality rates. Physicians should consider these infections as possible emergencies with a high risk for organ system failure and shock.

scholarly journals Cryptococcus Species Other Than Cryptococcus neoformans and Cryptococcus gattii: Are They Clinically Significant?

2020 ◽  
Vol 7 (12) ◽  
Author(s):  
Edison J Cano ◽  
Zachary A Yetmar ◽  
Raymund R Razonable
Keyword(s):  
Cryptococcus Neoformans ◽  
Opportunistic Infections ◽  
Descriptive Analysis ◽  
Laboratory Data ◽  
Invasive Disease ◽  
Cryptococcus Gattii ◽  
Cryptococcus Laurentii ◽  
Cryptococcus Species ◽  
Clinically Significant ◽  
Cryptococcus Spp

Abstract Background Cryptococcus spp is a major cause of opportunistic infections in immunocompromised patients, primarily due to Cryptococcus neoformans and Cryptococcus gattii. There are occasional reports of other Cryptococcus species causing invasive human disease. However, their epidemiology and clinical significance are not fully defined. We sought to describe cases with cultures positive for Cryptococcus species other than C neoformans and C gattii. Methods A retrospective descriptive analysis of clinical and laboratory data of patients with cultures growing Cryptococcus species other than C neoformans and C gattii from November 2011 to February 2019 was performed. Three Mayo Clinic sites in Arizona, Florida, and Minnesota were included. Results From 176 cases with a culture growing Cryptococcus spp, 54 patients (30%) had a culture for Cryptococcus other than C neoformans and C gattii in the study time frame. The most common species were Cryptococcus magnus, Cryptococcus laurentii, and Cryptococcus ater. The organisms were isolated and identified in culture of bronchoalveolar lavage (11), skin (11), urine (7), oral (4), sinus (3), intraoperative soft tissue (3), sputum (2), synovial fluid (2), cerebrospinal fluid (2), and intravenous catheter (2), among others (7). Only 8 (15%) cases were considered to be potentially pathogenic, with 1 case of invasive disease. Antifungal treatment was fluconazole, itraconazole, and griseofulvin, for a mean systemic antifungal duration of 42 days. Conclusions This large series of patients with Cryptococcus spp other than C neoformans and C gattii suggests that these species rarely cause clinically significant infection in humans. Only 1 case of invasive disease was found.

scholarly journals 855. Significance of Invasive Infections due to Methicillin Sensitive Staphylococcus aureus in the neonatal population

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S468-S468
Author(s):  
Mariawy Riollano ◽  
Deena Altman ◽  
shanna kowalsky ◽  
Stephanie Pan
Keyword(s):  
Staphylococcus Aureus ◽  
Clinical Characteristics ◽  
Invasive Disease ◽  
Control Measures ◽  
Invasive Infection ◽  
Single Center ◽  
Infection Control Measures ◽  
Invasive Infections ◽  
Mrsa Colonization ◽  
Neonatal Population

Abstract Background Staphylococcus aureus is a well-known cause of hospital acquired infections. Methicillin resistant staphylococcus aureus (MRSA) colonization is a recognized risk factor for invasive infections. The neonatal population in the intensive care unit (NICU) is particularly vulnerable to these types of infections, resulting in high mortality and morbidity. However, only scant data is available to establish the risk for invasive disease in patients with Methicillin sensitive staphylococcus aureus (MSSA). As a result, surveillance and prevention strategies are only address for MRSA colonization. Here, we describe the clinical characteristics of S. aureus colonized patients identified in late 2018 during transmission events in a single center NICU. As a result of the targeted surveillance investigation for MRSA infection control measures, S. aureus colonization was stratified, and we were able to compare the differences in invasive disease between MRSA and MSSA. Methods This is a retrospective chart review of the 47 colonized patients identified during October 2018- January 2019 SA transmission events in single center NICU. Risk factors, clinical characteristics, and the hospital course of these cases, including the proportion of invasive illness were reviewed. Results We found that most clinical characteristic, risk factors, and hospital course were the same between MRSA and MSSA colonized infants (p values &gt; 0.05). Additionally, there was no difference in the proportion of invasive infection between MRSA and MSSA colonized patients (p value &gt; 0.05). The type of invasive infections identified were SSTI, bacteremia, and osteomyelitis. Conclusion The proportion of invasive infection was the same in MSSA and MRSA colonized patients. This data provides us with supportive material for future recommendations of infection control measures for MSSA colonized patients. Disclosures All Authors: No reported disclosures

scholarly journals Detection and Classification of Cancer from Microscopic Biopsy Images Using Clinically Significant and Biologically Interpretable Features

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Rajesh Kumar ◽  
Rajeev Srivastava ◽  
Subodh Srivastava
Keyword(s):  
Texture Features ◽  
Histogram Equalization ◽  
Gray Level ◽  
Segmentation Methods ◽  
Segmented Images ◽  
Clinically Significant ◽  
Classification Of Images ◽  
Nearest Neighborhood ◽  
Neighborhood Method

A framework for automated detection and classification of cancer from microscopic biopsy images using clinically significant and biologically interpretable features is proposed and examined. The various stages involved in the proposed methodology include enhancement of microscopic images, segmentation of background cells, features extraction, and finally the classification. An appropriate and efficient method is employed in each of the design steps of the proposed framework after making a comparative analysis of commonly used method in each category. For highlighting the details of the tissue and structures, the contrast limited adaptive histogram equalization approach is used. For the segmentation of background cells, k-means segmentation algorithm is used because it performs better in comparison to other commonly used segmentation methods. In feature extraction phase, it is proposed to extract various biologically interpretable and clinically significant shapes as well as morphology based features from the segmented images. These include gray level texture features, color based features, color gray level texture features, Law’s Texture Energy based features, Tamura’s features, and wavelet features. Finally, the K-nearest neighborhood method is used for classification of images into normal and cancerous categories because it is performing better in comparison to other commonly used methods for this application. The performance of the proposed framework is evaluated using well-known parameters for four fundamental tissues (connective, epithelial, muscular, and nervous) of randomly selected 1000 microscopic biopsy images.

Ocular Tuberculosis: Clinics, Diagnosis and Treatment (Literature Review)

The Eye ◽  
2021 ◽  
Vol 23 (2) ◽  
pp. 41-45
Author(s):  
O. E. Russkikh ◽  
N. Y. Nikolenko
Keyword(s):  
Literature Review ◽  
Diagnostic Methods ◽  
Diagnosis And Treatment ◽  
Ocular Tuberculosis ◽  
Treatment Regimens ◽  
The Russian Federation ◽  
Serious Disease ◽  
Suggested Classification

Ocular tuberculosis is a serious disease with a long recurrent course, often leading to a significant decrease in the visual functions and quality of life of patients as well as disability. In recent years, the incidence rate of the ocular tuberculosis in the Russian Federation has been declining: in 2016, according to a number of authors, its incidence amounted to 5.2%.Purpose. To systematize literature data on the topic of ocular tuberculosis.Materials and methods. We reviewed literature available on elibrary.ru, cyberleninka.ru websites and in “Clinical Ophthalmology” journal. Results. We suggested classification of the ocular tuberculosis lesions, analyzed modern diagnostic methods and treatment regimens and evaluated their effectiveness.Conclusions. Literature review revealed that in order to improve the quality of detection, diagnosis and treatment of ocular tuberculosis, joint efforts of ophthalmologists and phthisiologists as well as use of modern methods of tuberculinodiagnosis are essential.

Assessing Reliable and Clinically Significant Change on Health of the Nation Outcome Scales: Method for Displaying Longitudinal Data

2005 ◽  
Vol 39 (8) ◽  
pp. 719-724 ◽  
Author(s):  
Alberto Parabiaghi ◽  
Angelo Barbato ◽  
Barbara D'avanzo ◽  
Arcadio Erlicher ◽  
Antonio Lora
Keyword(s):  
Clinical Improvement ◽  
Normative Data ◽  
Community Mental Health Services ◽  
Routine Data ◽  
Clinically Significant Change ◽  
Real Change ◽  
Significant Change ◽  
Clinically Significant ◽  
Routine Data Collection

Objective: Many authors recommended that reliable and clinically significant change (RCSC) should be calculated when reporting results of interventions. To test the reliability of the Health of the Nation Outcome Scales (HoNOS) in identifying RCSC, we applied the Jacobson and Truax model to two HoNOS assessments in a large group of people evaluated in 10 community mental health services in Lombardy, Italy, in 2000. Method: The HoNOS was administered to 9817 patients; of these, 4759 (48%) were re-assessed. Reliable change (RC) was calculated using Cronbach's alpha ( α), as a parameter of the reliability of the measure. Clinical significance cut-offs were calculated using a classification of severity based on HoNOS items. Results: In the whole sample, the clinical improvement cut-off was 11 and the remission cut-off was 5. Considering the severe patients, the clinical improvement cut-off was 12. The RC index calculated on the whole group and on the subgroup of severe patients indicated that eight-point and seven-point changes, respectively, were needed to be confident that a real change had occurred. Longitudinal changes were depicted on two-dimensional graphs as examples of reporting RCSC on HoNOS total scores in a routine data collection: 91.6% of the whole sample (4361) was stable, 5.6% (269) improved and 1.8% (129) worsened. Conclusion: Our study proposes a methodological framework for computing RCSC normative data on a widely used outcome scale and for identifying different degrees of clinical change.

scholarly journals A Comparative Study on Application of Computer Vision and Fluorescence Imaging Spectroscopy for Detection of Huanglongbing Citrus Disease in the USA and Brazil

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Caio B. Wetterich ◽  
Ratnesh Kumar ◽  
Sindhuja Sankaran ◽  
José Belasque Junior ◽  
Reza Ehsani ◽  
...  
Keyword(s):  
Computer Vision ◽  
Fluorescence Imaging ◽  
Imaging Spectroscopy ◽  
Texture Features ◽  
Support Vector ◽  
Infected Leaf ◽  
Segmented Images ◽  
The Usa ◽  
Citrus Disease

The overall objective of this work was to develop and evaluate computer vision and machine learning technique for classification of Huanglongbing-(HLB)-infected and healthy leaves using fluorescence imaging spectroscopy. The fluorescence images were segmented using normalized graph cut, and texture features were extracted from the segmented images using cooccurrence matrix. The extracted features were used as an input into the classifier, support vector machine (SVM). The classification results were evaluated based on classification accuracies and number of false positives and false negatives. The results indicated that the SVM could classify HLB-infected leaf fluorescence intensities with up to 90% classification accuracy. Though the fluorescence intensities from leaves collected in Brazil and the USA were different, the method shows potential for detecting HLB.

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