scholarly journals Cell Hydration as a Biomarker for Estimation of Biological Effects of Nonionizing Radiation on Cells and Organisms

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Sinerik Ayrapetyan ◽  
Jaysankar De
Keyword(s):  
Temperature Sensitivity ◽  
Biological Effects ◽  
Mechanical Vibration ◽  
Low Frequency ◽  
Water Structure ◽  
Water Dynamics ◽  
Osmotic Gradient ◽  
Dormant State ◽  
Structure Changes ◽  
Nonionizing Radiation

“Changes in cell hydration” have been hypothesized as an input signal for intracellular metabolic cascade responsible for biological effects of nonionizing radiation (NIR). To test this hypothesis a comparative study on the impacts of different temperature and NIR (infrasound frequency mechanical vibration (MV), static magnetic field (SMF), extremely low frequency electromagnetic field (ELF EMF), and microwave (MW)) pretreated water on the hydration of barley seeds in its dormant and germination periods was performed. In dormant state temperature sensitivity (Q10) of seed hydration in distilled water (DW) was less than 2, and it was nonsensitive to NIR treated DW, whereas during the germination period (48–72 hours) seeds hydration exhibited temperature sensitivityQ10>2and higher sensitivity to NIR treated DW. Obtained data allow us to suggest that the metabolic driving of intracellular water dynamics accompanied by hydrogen bonding and breaking is more sensitive to NIR-induced water structure changes in seed bathing aqua medium than the simple thermodynamic processes such as osmotic gradient driven water absorption by seeds in dormant state. Therefore, cell hydration is suggested to be a universal and extrasensitive biomarker for detection of biological effects of NIR on cells and organisms.

scholarly journals The Relationship between Vibratory Sensation and Body Surface Vibration Induced by Low-Frequency Noise

2002 ◽  
Vol 21 (2) ◽  
pp. 87-100 ◽  
Author(s):  
Yukio Takahashi ◽  
Kazuo Kanada ◽  
Yoshiharu Yonekawa
Keyword(s):  
Body Surface ◽  
Mechanical Vibration ◽  
Low Frequency ◽  
The Body ◽  
Frequency Noise ◽  
Low Frequency Noise ◽  
Vibration Acceleration ◽  
Surface Vibration ◽  
Human Body Surface ◽  
The Relationship

Human body surface vibration induced by low-frequency noise was measured at the forehead, the chest and the abdomen. At the same time, subjects rated their vibratory sensation at each of these locations. The relationship between the measured vibration on the body surface and the rated vibratory sensation was examined, revealing that the vibratory sensations perceived in the chest and abdomen correlated closely with the vibration acceleration levels of the body surface vibration. This suggested that a person exposed to low-frequency noise perceives vibration at the chest or abdomen by sensing the mechanical vibration that the noise induces in the body. At the head, on the other hand, it was found that the vibratory sensation correlated comparably with the vibration acceleration level of the body surface vibration and the sound pressure level of the noise stimulus. This finding suggested that the mechanism of perception of vibration in the head is different from that of the perception of vibratory sensation in the chest and the abdomen.

Aerodynamic Drag Measurement in a High-Enthalpy Shock Tunnel

2018 ◽  
Author(s):  
Yunpeng Wang ◽  
Zonglin Jiang ◽  
Honghui Teng
Keyword(s):  
Natural Frequencies ◽  
Aerodynamic Drag ◽  
Aerodynamic Force ◽  
Mechanical Vibration ◽  
Low Frequency ◽  
Shock Tunnel ◽  
Test Time ◽  
Test Duration ◽  
Flow State ◽  
High Enthalpy

Shock tunnels create very high temperature and pressure in the nozzle plenum and flight velocities up to Mach 20 can be simulated for aerodynamic testing of chemically reacting flows. However, this application is limited due to milliseconds of its test duration (generally 500 μs–20 ms). For the force test in the conventional hypersonic shock tunnel, because of the instantaneous flowfield and the short test time [1–4], the mechanical vibration of the model-balance-support (MBS) system occurs and cannot be damped during a shock tunnel run. The inertial forces lead to low frequency vibrations of the model and its motion cannot be addressed through digital filtering. This implies restriction on the model’s size and mass as its natural frequencies are inversely proportional the length scale of the model. As to the MBS system, sometimes, the lowest natural frequency of 1 kHz is required for the test time of typically 5 ms in order to get better measurement results [2]. The higher the natural frequencies, the better the justification for the neglected acceleration compensation. However, that is very harsh conditions to design a high-stiffness MBS structure, particularly a drag balance. Therefore, it is very hard to carried out the aerodynamic force test using traditional wind tunnel balances in the shock tunnel, though its test flow state with the high-enthalpy is closer to the real flight condition.

scholarly journals Testing Time and Frequency Fiber-Optic Link Transfer by Hardware Emulation of Acoustic-Band Optical Noise

2016 ◽  
Vol 23 (2) ◽  
pp. 309-316
Author(s):  
Marcin Lipiński ◽  
Przemysław Krehlik ◽  
Łukasz Śliwczyński ◽  
Łukasz Buczek ◽  
Jacek Kołodziej
Keyword(s):  
Fiber Optic ◽  
Data Transfer ◽  
Acoustic Noise ◽  
Mechanical Vibration ◽  
Low Frequency ◽  
Propagation Delay ◽  
Optical Signal ◽  
Testing Time ◽  
Optical Noise ◽  
Signal Phase

Abstract The low-frequency optical-signal phase noise induced by mechanical vibration of the base occurs in field-deployed fibers. Typical telecommunication data transfer is insensitive to this type of noise but the phenomenon may influence links dedicated to precise Time and Frequency (T&F) fiber-optic transfer that exploit the idea of stabilization of phase or propagation delay of the link. To measure effectiveness of suppression of acoustic noise in such a link, a dedicated measurement setup is necessary. The setup should enable to introduce a low-frequency phase corruption to the optical signal in a controllable way. In the paper, a concept of a setup in which the mechanically induced acoustic-band optical signal phase corruption is described and its own features and measured parameters are presented. Next, the experimental measurement results of the T&F transfer TFTS-2 system’s immunity as a function of the fibre-optic length vs. the acoustic-band noise are presented. Then, the dependency of the system immunity on the location of a noise source along the link is also pointed out.

scholarly journals Biological Effects of a Low-Frequency Electromagnetic Field on Yeast Cells of the Genus Saccharomyces Cerevisiae

2021 ◽  
Vol 21 (2) ◽  
pp. 34-41
Author(s):  
K Sladicekova ◽  
M Bereta ◽  
J Misek ◽  
D Parizek ◽  
J Jakus
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Electromagnetic Field ◽  
Magnetic Flux ◽  
Flux Density ◽  
Biological Effects ◽  
Low Frequency ◽  
Growth Dynamics ◽  
Yeast Cells ◽  
Magnetic Flux Density ◽  
Time Points

Abstract Background: Although the scientific community is extensively concerned with the effects of the EMF, the unambiguous explanation of its effects on living structures is still lacking. Goals: The goal of the study was to evaluate the effect of a low-frequency (LF) electromagnetic field (EMF) on the growth and multiplication of the yeast Saccharomyces cerevisiae. Methods: Yeast cells were exposed to a frequency of 900 Hz and a magnetic flux density of 2.3 mT. The duration of each experiment was 8 hours, in the beginning of the measurement the value of frequency, rms (root mean square) value of electric current (2 A), and magnetic flux density were fixed set on the exposure device. A paired experiment was performed, a sample exposed to EMF, and a sample shielded from the field. Subsequently, samples were taken every two hours, the number of cells was recorded, and then the concentration of the yeast cells was evaluated at time points. The time points reflected the exposure time of the samples exposed to EMF. Results: The results indicate that LF EMF at given parameters has an inhibitory effect on the growth and multiplication of yeast cells. Conclusion: Exposure to EMF can cause the differences in growth dynamics between cells exposed to the field and the unexposed ones.

scholarly journals Identification and Removal of Non-acoustic Noise in Towed Array Sonar Using F-Κ Transform for Enhanced Torpedo Detection

2020 ◽  
Vol 14 ◽  
Keyword(s):  
Vibration Isolation ◽  
Acoustic Noise ◽  
Detection System ◽  
Mechanical Vibration ◽  
Low Frequency ◽  
Acoustic Energy ◽  
Noise Sources ◽  
Towed Array ◽  
Spatio Temporal ◽  
Towed Arrays

Low frequency passive towed array sonar is an essential component in a torpedo detection system for surface ships. Compact towed arrays are used for torpedo detection and they will be towed at higher towing speeds compared to conventional towed array sonars used for surveillance. Presence of non-acoustic noise in towed array sensors at higher towing speeds degrades torpedo detection capability at lower frequencies. High wavenumber mechanical vibrations are induced in the array by vortex shedding associated with hydrodynamic flow over the array body and cable scope. These vibrations are known to couple into the hydrophone array as nonacoustic noise sources and can impair acoustic detection performance, particularly in the forward end fire direction. Lengthy mechanical vibration isolation modules can isolate vibration induced noise in towed arrays, but this is not recommended in a towed array which is towed at high speeds as it will increase the drag and system complexity. An algorithm for decomposing acoustic and non-acoustic components of signals received at sensor level using well known frequency-wavenumber transform (F-K transform) is presented here. Frequency-wavenumber diagrams can be used for differentiating between acoustic and non-acoustic signals. An area of V shape is identified within the F-K spectrum where acoustic energy is confined. Energy outside this V will highlight non-acoustic energy. Enhanced simultaneous spatio-temporal and spatio-amplitude detection is possible with this algorithm. Performance of this algorithm is validated through simulation and experimental data.

A novel magnetic field generating machine with a wide range of tunable parameters to study in vitro cells

2020 ◽  
Author(s):  
Maryam sadat Nezamtaheri ◽  
Seyed Peyman Shariatpanahi ◽  
Bahram Goliaei ◽  
Alireza Madjid Ansari
Keyword(s):  
Magnetic Field ◽  
Cancer Cells ◽  
Biological Effects ◽  
Low Frequency ◽  
Live Cells ◽  
Magnetic Flux Density ◽  
Dependent Manner ◽  
Exposure System ◽  
Wide Range ◽  
Frequency Magnetic Field

Abstract Background Extremely low-frequency magnetic field (ELF-MF) significantly induces apoptosis in cancer cells. To study the biological effects of the ELF-MF on tumor cells, we have designed and constructed a new exposure system that provides a uniform magnetic field with negligible temperature fluctuations during the exposure. Additionally, it provides ideal physiological conditions for live cells inside the incubator. This ELF-MF exposure system eliminates several limitations and disadvantages of other low frequency magnetic field systems; it generates a magnetic field with a frequency of 0 to 70 Hz with a maximum magnetic flux density of 150 mT. Methods The capabilities of the setup were examined using a 1 Hz, 100 mT magnetic field, at various exposure times, to induce apoptosis-mediated cell death in the MC4-L2 cell line. After exposure, apoptosis was assessed by flow cytometry. Results A biphasic response was observed in cells exposed to ELF-MF: at first a decreasing apoptotic rate during 2-12 hours exposure time was detected, after which apoptosis gradually increased during 24-120 hours of exposure. Conclusions We show that ELF-MF exposure with a frequency of 1 Hz and intensity of 100 mT induces apoptosis in MC4-L2 cancer cells in a time-dependent manner. These results show the significance of the long term studies of the ELF exposure effects.

scholarly journals Low‐frequency mechanical vibration induces apoptosis of A431 epidermoid carcinoma cells

2020 ◽  
Vol 20 (7) ◽  
pp. 232-238
Author(s):  
Wresti L. Anggayasti ◽  
Chikahiro Imashiro ◽  
Taiki Kuribara ◽  
Kiichiro Totani ◽  
Kenjiro Takemura
Keyword(s):  
Mechanical Vibration ◽  
Low Frequency ◽  
Carcinoma Cells ◽  
Epidermoid Carcinoma
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