scholarly journals Bacterial Biodegradation of Crude Oil Using Local Isolates

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Raed S. Al-Wasify ◽  
Shimaa R. Hamed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Crude Oil ◽  
Oil Spills ◽  
Bacterial Species ◽  
Bacterial Consortium ◽  
Contaminated Water ◽  
Water And Soil Samples ◽  
Bacterial Biodegradation ◽  
Mixed Bacterial Consortium

An experimental study was undertaken to assess the efficiency of Pseudomonas aeruginosa, Bacillus subtilis, and Acinetobacter lwoffi isolated from petroleum contaminated water and soil samples to degrade crude oil, separately and in a mixed bacterial consortium. Capillary gas chromatography was used for testing the effect of those bacterial species on the biodegradation of crude oil. Individual bacterial cultures showed less growth and degradation than did the mixed bacterial consortium. At temperature 22°C, the mixed bacterial consortium degraded a maximum of 88.5% of Egyptian crude oil after 28 days of incubation. This was followed by 77.8% by Pseudomonas aeruginosa, 76.7% by Bacillus subtilis, and 74.3% by Acinetobacter lwoffi. The results demonstrated that the selected bacterial isolates could be effective in biodegradation of oil spills individually and showed better biodegradation abilities when they are used together in mixed consortium.

Biodegradation of crude oil by a defined co-culture of indigenous bacterial consortium and exogenous Bacillus subtilis

2017 ◽  
Vol 224 ◽  
pp. 327-332 ◽  
Author(s):  
Kaiyun Tao ◽  
Xiaoyan Liu ◽  
Xueping Chen ◽  
Xiaoxin Hu ◽  
Liya Cao ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Crude Oil ◽  
Bacterial Consortium

Study of the reduction of aliphatic and aromatic fractions of crude oil-contaminated water by a bacterial consortium

2010 ◽  
Vol 150 ◽  
pp. 212-213
Author(s):  
Dariush Minai-Tehrani ◽  
Saeed Minoui ◽  
Zahra Sobhani-Damavandifar
Keyword(s):  
Crude Oil ◽  
Bacterial Consortium ◽  
Contaminated Water

scholarly journals Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

2018 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Bacterial Species ◽  
Nutritional Stress ◽  
Cellular Level ◽  
Bacterial Cells ◽  
Loss Of Function ◽  
Swarming Motility ◽  
Bacteria Growth ◽  
Solid Media

Coping with nutritional stress is essential for cell survival, of which many strategies at the cellular level lend support for ensuring the survival of the population at a particular habitat. One postulated mechanism is swarming motility in bacterial cells, where, upon depletion of nutrients at a locale, cells would coordinate their movement, synthesize more flagella, and secrete lubricants for moving rapidly across surfaces in search for food. Known to engage in swarming motility, Bacillus subtilis and Pseudomonas aeruginosa are two common bacterial species with versatile metabolism that use the motility mode to colonize new habitats with more favourable environmental and nutritional conditions. However, experimental observations of bacteria growth on a variety of agar media revealed that B. subtilis NRS-762 (ATCC 8473) and P. aeruginosa PRD-10 (ATCC 15442) exhibited retarded swarming motility upon entry into stationary phase on solid media. Specifically, B. subtilis NRS-762 colonies exhibited round, wrinkled morphologies compared to complex filamented swarming patterns common in strains able to engage in swarming motility. On the other hand, P. aeruginosa PRD-10 colonies were round, mucoid, and expanded outwards from the colony centre without extending filaments from the centre; thereby, indicating retarded swarming motility. Thus, impaired cellular machinery for swarming motility or mutated and deleted genes likely account for observed retarded swarming motility in B. subtilis NRS-762 and P. aeruginosa PRD-10. More importantly, observations of small filaments extending radially from an expanded colony of P. aeruginosa PRD-10 grown on minimal salts medium supplemented with yeast extract highlighted possible loss of function of effector molecules that transmit cellular decision at swarming motility into movement, while sensory mechanisms feeding into the motility mechanism remained intact. More broadly, observations of impaired swarming motility in B. subtilis NRS-762 and P. aeruginosa PRD-10 in two species otherwise endowed with the motility mode highlighted that additional triggers for swarming motility are likely present, and the motility mode may have been evolutionary selected for other functions in addition to foraging for food in times of nutritional stress.

scholarly journals Long-Term Combined Effects of Crude Oil and Dispersant on Sediment Bacterial Community

2020 ◽  
Vol 9 (1) ◽  
pp. 259-263
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Crude Oil ◽  
Bacterial Community Structure ◽  
Oil Spills ◽  
Bacterial Species ◽  
Combined Effects ◽  
Set Up ◽  
Sediment Bacterial Community

To better understand long-term combined effects of crude oil and dispersant on bacterial community, sediments microcosms were set up in triplicates and treated with dispersant (Corexit 9500A), crude oil, and Corexit 9500A plus crude oil. After 60 days exposure, there was a significant change in the bacterial community structure in all treatments. The shift in the bacterial community structure in Corexit 9500A plus crude oil treatment was considerably different from those by either Corexit 9500A or crude oil. DNA sequence analysis showed that Hydrocarboniphaga effuse, Parvibaculum lavamentivorans,and Alicyclobacillus ferrooxydans were the major bacterial species in crude oil treatment. Pandoraea thiooxydans, Janthinobacterium sp. and Hyphomicrobium nitrativorans were the most dominant species in Corexit 9500A treatment. The species Janthinobacterium sp., Parvibaculum lavamentivorans, and Dyella sp. were enriched in Corexit 9500A plus crude oil treatment. The majority of the detected species were hydrocarbons degraders. The study showed that Corexit 9500A addition enhanced the biodegradation rate by increasing the diversity and richness of hydrocarbons degrading species. Corexit A9500 application should be considered during crude oil spills to evaluate environmental impacts.

Surfactant-enhanced biodegradation of crude oil by mixed bacterial consortium in contaminated soil

2018 ◽  
Vol 25 (15) ◽  
pp. 14437-14446 ◽  
Author(s):  
Ruiwei Xu ◽  
Zhen Zhang ◽  
Liping Wang ◽  
Ningning Yin ◽  
Xiaohui Zhan
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Bacterial Consortium ◽  
Enhanced Biodegradation ◽  
Mixed Bacterial Consortium

Study Potential of Indigenous Pseudomonas aeruginosa and Bacillus subtilis in Bioremediation of Diesel-Contaminated Water

2016 ◽  
Vol 228 (1) ◽  
Author(s):  
Mohammad-Saeed Safdari ◽  
Hamid-Reza Kariminia ◽  
Zahra Ghobadi Nejad ◽  
Thomas H. Fletcher
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Contaminated Water

scholarly journals Biodegradation Potentials of Bacterial Isolates from Auto-Mechanic Workshops in Oluku, Edo State, Nigeria

2017 ◽  
Vol 1 (2) ◽  
pp. 256-264
Author(s):  
C. E. Oshoma ◽  
E. E. Eze ◽  
S. E. Omonigho
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Klebsiella Oxytoca ◽  
Bacterial Consortium ◽  
Petroleum Products ◽  
Bacterial Isolates ◽  
E Coli ◽  
Edo State

The frequent discharge of used petroleum products from automobiles has become a major source of concern due to unguided discharge into the soil environment, hence the need for biodegradation of the products. Bacterial species were isolated from contaminated soil in mechanic workshops and screened for their for hydrocarbon degradation potentials using standard microbiological procedures. Physicochemical properties of the contaminated soil were also analysed using standard techniques. The highest and lowest heterotrophic bacterial counts of 2.82 ± 0.16 ×108 and 2.09 ± 0.32 ×108 cfu/g were from the control soil and site 1 respectively. For hydrocarbon utilizing bacterial, Site 2 had the highest load of 8.33 ± 2.55 ×105 while the control had the least 1.35±0.33× 104 cfu/g. The bacterial isolates from the contaminated soil were found to be Corynebacterium kutsceri, Escherichia coli, Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Klebsiella oxytoca, Staphylococcus aureus, Pseudomonas aeruginosa and Micrococcus luteus. The highest and lowest in the frequency of occurrence among the isolates were B. subtilis (27.5 %) and E. coli (1.4%) respectively. The screened hydrocarbon utilizing bacterial isolates were C. kutseri, B. subtilis and P. aeruginosa. The ability to degrade crude oil revealed that bacterial consortium had the highest growth profile of 12.90 ×105 while the least was C. kutsceri with values of 8.20 ×105 cfu/g. The consortium bacteria had the highest percentage of hydrocarbon products degradation. The ability of the consortium bacteria to remove a high percentage of crude oil components makes it potentially useful for bioremediation of site highly contaminated with petroleum hydrocarbon.

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