scholarly journals Stimulatory Effects of Gamma Irradiation on Phytochemical Properties, Mitotic Behaviour, and Nutritional Composition of Sainfoin (Onobrychis viciifoliaScop.)

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Sadegh Mohajer ◽  
Rosna Mat Taha ◽  
Ma Ma Lay ◽  
Arash Khorasani Esmaeili ◽  
Mahsa Khalili
Keyword(s):  
Gamma Irradiation ◽  
Nutritional Composition ◽  
Anthocyanin Content ◽  
Irradiation Intensity ◽  
Dry Matter Digestibility ◽  
Chromosome Bridge ◽  
Hplc Profiles ◽  
Positive Correlations ◽  
Gamma Irradiated

Sainfoin (Onobrychis viciifoliaScop. Syn.Onobrychis sativaL.) is a bloat-safe forage crop with high levels of tannins, which is renowned for its medicinal qualities in grazing animals. Mutagenesis technique was applied to investigate the influence of gamma irradiation at 30, 60, 90, and 120 Gy on mitotic behavior,in vitrogrowth factors, phytochemical and nutritional constituents of sainfoin. Although a percentage of plant necrosis and non-growing seed were enhanced by irradiation increment, the germination speed was significantly decreased. It was observed that gamma irradiated seeds had higher value of crude protein and dry matter digestibility compared to control seeds. Toxicity of copper was reduced in sainfoin irradiated seeds at different doses of gamma rays. Anthocyanin content also decreased in inverse proportion to irradiation intensity. Accumulation of phenolic and flavonoid compounds was enhanced by gamma irradiation exposure in leaf cells. HPLC profiles differed in peak areas of the two important alkaloids, Berberine and Sanguinarine, in 120 Gy irradiated seeds compared to control seeds. There were positive correlations between irradiation dose and some abnormality divisions such as laggard chromosome, micronucleus, binucleated cells, chromosome bridge, and cytomixis. In reality, radiocytological evaluation was proven to be essential in deducing the effectiveness of gamma irradiation to induce somaclonal variation in sainfoin.

scholarly journals Chemical composition and in vitro digestibility of some selected under-utilized tropical seeds as protein sources in ruminants’ diet

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Oluwatosin Bode Omotoso ◽  
Mary Oluwafunmilayo Adeduntan ◽  
Adebowale Noah Fajemisin
Keyword(s):  
Short Chain Fatty Acids ◽  
Nutritional Composition ◽  
Metabolizable Energy ◽  
In Vitro Digestibility ◽  
Protein Sources ◽  
Dry Matter Digestibility ◽  
Supplementary Feed ◽  
Monodora Myristica

Abstract Background The study highlighted the potential of three common and under-utilized tropical leguminous seeds (Tomentosa nilotica, Dioclea reflexa and Monodora myristica) to be used as supplementary feed to ruminant livestock. These seeds (their plants inclusive) are valuable sources of food and medicine for the prevention of illness and maintenance of human health. The medicinal properties of these seeds include antimicrobial, anti-inflammatory, anti-oxidant and immuno-stimulant. Trypsin inhibitors, which are common anti-nutritional factors in legumes and for monogastric animals, do not exert adverse effects in ruminants because they are degraded in the rumen. Hence, the crux of this study is to examine the effect of processing methods on the nutritional composition (proximate, fibre fractions, minerals, anti-nutrients) and in vitro digestibility of Tomentosa nilotica, Dioclea reflexa and Monodora myristica seeds and their suitability as feedstuff (protein sources) in small ruminant feed, particularly during off-season. Results From the results, raw Tomentosa nilotica and Monodora myristica have the highest crude protein (30.35% CP) and fat (22.40% EE), respectively. It is noteworthy that roasting best improve the mineral and significantly reduce the anti-nutrients observed in this study better compared to boiling and soaking methods. The highest organic matter digestibility, short-chain fatty acids, metabolizable energy and in vitro dry matter digestibility values were obtained in Dioclea reflexa compared to other test seeds. Roasting best improved the nutritive values, while Dioclea reflexa seed was rated highest for all the nutritional attributes and in vitro digestibility. Conclusions Dioclea reflexa could be incorporated in ruminants’ diet as protein source, particularly during the off-season, for improved ruminant production in Nigeria. However, in vivo study is therefore recommended to validate this report.

Antinutritional factors, ruminal degradation and in vitro protein digestibility of gamma irradiated full-fat soybean

2009 ◽  
Vol 2009 ◽  
pp. 184-184
Author(s):  
M Taghinejad Roudbaneh ◽  
A Nikkhah ◽  
A A Sadeghi ◽  
G Raisali ◽  
M Chamani
Keyword(s):  
Gamma Irradiation ◽  
Dairy Cows ◽  
Nutritional Value ◽  
Protein Digestibility ◽  
High Energy ◽  
Antinutritional Factors ◽  
Γ Irradiation ◽  
Rumen Microbes ◽  
Gamma Irradiated

Whole soybean is used as a high energy-protein supplement for dairy cows, but contains various antinutritional factors and the protein is highly degradable by rumen microbes. Roasting and extrusion are very popular ways of feeding soybeans to dairy cows but these processing methods may adversely affect the protein digestibility and lysine availability of the final product in the small intestine. Gamma irradiation has been recognized as a reliable and safe method for improving the nutritional value and inactivation or removal of certain antinutritional factors in foods and feeds (Siddhuraju et al., 2002). As far as we know, information about the effects of γ-irradiation on ruminal crude protein (CP) degradation of full-fat soybean in the literature is scarce. The aim of this study was to evaluate the effects of gamma irradiation (γ-irradiation) on nutritional value and antinutritional factors of soybean (SB) for ruminants.

The genetic relationship between the digestibility of leaf, stem and whole plant in Setaria

1974 ◽  
Vol 25 (3) ◽  
pp. 401 ◽  
Author(s):  
JB Hacker
Keyword(s):  
Ploidy Level ◽  
Genetic Relationship ◽  
Dry Matter ◽  
Genetic Correlations ◽  
Dry Matter Digestibility ◽  
Whole Plant ◽  
Genotypic And Phenotypic Correlations ◽  
Selection For ◽  
Positive Correlations

The genetic interrelationships of in vitro dry matter digestibility of whole plant (DMDWP), leaf (DMDL), stem (DMDS) and terminal two leaf (DMD2L) fractions were investigated in 4-week regrowth of 21 tetraploid clones of Setaria anceps and 14 hexaploid clones of Setaria trinervia. There were significant differences between genotypes of more than eight digestibility units for all fractions, and for all fractions significant positive correlations were obtained between genotype performance at successive harvests. Genotypic and phenotypic correlations between leaf and stem digestibility were mainly positive, but generally low. Genetic correlations for DMDL–DMDWP and DMDS–DMDWP mostly exceeded 0.7 and for DMD2L–DMDL 0.6. Markedly lower values were obtained for the DMD2L–DMDWP comparison. Genotypic correlations for DMDWP-percentage leaf were greater than 0.4 at one harvest. It is concluded that selection for high DMDWP should result in improvement for both leaf and stem digestibility, providing selection was within populations at the same ploidy level. There appears to be little justification for basing selection on the terminal two-leaf fraction, in view of the relatively inconsistent ranking of genotypes in the two successive harvests, and the relatively low genetic correlations between digestibility of this fraction and that of the whole plant.

309 DIFFERENCES IN PRONUCLEUS FORMATION RATES BETWEEN BULLS IN RESPONSE TO GAMMA IRRADIATION OF FROZEN - THAWED SEMEN

2007 ◽  
Vol 19 (1) ◽  
pp. 270
Author(s):  
M. M. Petersen ◽  
G. B. Boe-Hansen ◽  
A. Birck ◽  
B. Avery ◽  
M. Jensen ◽  
...  
Keyword(s):  
Dna Damage ◽  
Gamma Irradiation ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Fertilization Rate ◽  
High Dose ◽  
Exact Test ◽  
Bovine Spermatozoa ◽  
Gamma Irradiated

Gamma irradiation induces DNA damage to mature bovine spermatozoa but does not affect motility, membrane integrity, or function (Fatehi et al. 2006 J. Androl. 27, 176–188), making it a useful model to evaluate the effect of fertilization with DNA-damaged spermatozoa. The objective of the present study was to analyze the fertilizing capacity of frozen–thawed gamma-irradiated sperm from 2 bulls: a bull with a high sperm quality and fertilization rate (bull A), and a bull with a low sperm quality and fertilization rate (bull B). To ensure that DNA damage was induced, frozen–thawed semen was exposed to a high dose of irradiation (90 Gy). Cumulus–oocyte complexes were obtained from abattoir ovaries and were in vitro-matured (IVM) using standard procedures (23 h in DMEM with 5% serum and eCG/hCG). For each treatment group, 3 to 5 straws of semen from the same ejaculate were used. After thawing, semen from the 2 bulls was either irradiated or held at room temperature before 1:1 dilution in sperm-TALP. During irradiation, semen was kept in the straws. The irradiated and nonirradiated sperm were used for IVF (23 h in IVF-TALP) within 1 h of thawing. IVM and IVF were carried out at 38.5�C in 5% CO2 in air. After IVF, presumptive zygotes were whole mount-fixed and 24 h later were stained with 1% aceto-orcein followed by determination of fertilization status. Fertilization was considered normal if 2 pronuclei (PN) were present. A total of 4 replicates were performed and 286 zygotes analyzed. No difference was detected between replicates, and the results were pooled. Fisher's exact test was used to determine effect of treatment. More zygotes had 2 PN after fertilization with semen from bull A (47/67 = 70%), compared with that from bull B (16/71 = 23%; P < 0.0001) using nonirradiated sperm. Irradiation of sperm significantly increased the fraction of zygotes with 2 PN from bull A (61/70 = 87%; P = 0.03), but decreased the 2 PN fractions in zygotes fertilized with sperm from bull B (2/75 = 3%; P = 0.0002). Ideally, to avoid straw variation, semen should have been pooled and divided into groups before irradiation. The variation between straws might explain the higher fertilization rates in bull A using irradiated sperm compared with nonirradiated sperm. In conclusion, there appear to be differences in fertilizing ability between bulls after irradiation of frozen–thawed sperm. This could be due to suboptimal DNA packaging, which made sperm from bull B more susceptible to radiation-induced damage. The potential irradiation-induced increase in DNA fragmentation in sperm from bull B compared with that from bull A might delay or prevent the formation of the 2 PN. Further studies are needed to investigate differences in fertilization and early embryonic development using sperm with intact or damaged DNA.

scholarly journals Properties of different silage maize hybrids

2020 ◽  
Vol 47 (2) ◽  
pp. 139-147
Author(s):  
Valentina Nikolić ◽  
Slađana Žilić ◽  
Milica Radosavljević ◽  
Jelena Vančetović ◽  
Sofija Božinović
Keyword(s):  
Dry Matter ◽  
Agronomic Traits ◽  
Correlation Coefficients ◽  
Nutritional Composition ◽  
Matter Content ◽  
Dry Matter Content ◽  
Maize Hybrids ◽  
Dry Matter Digestibility ◽  
Fibre Composition

The aim of this study was to observe the properties of ten maize hybrids from Serbia including agronomic traits, lignocellulosic fibre composition and in vitro dry matter digestibility. Five yellow kernel dent hybrids and five analogue red kernel hybrids were used in this study to evaluate the agronomic traits, dry matter content of the whole plant, lignocellulosic fibre composition, ratios between different fibres, in vitro dry matter and NDF digestibility. Correlation coefficients between the investigated traits of the maize hybrids were assessed. Even though the results of our study showed variations regarding nutritional composition of the whole maize plant between the investigated maize hybrids, the hybrid and the differences in kernel colour (yellow or red) did not considerably affect the properties that influence quality of the maize hybrids for silage production. The highest IVDMD was determined in yellow kernel hybrid ZP 388, while the maximum NDFD was detected in the red kernel hybrid ZP 606red which also showed the lowest ADL/NDF and ADF/NDF ratios. The results indicate that all of the hybrids used in this study are good candidates for the production of high-quality silage for ruminant nutrition.

The effect of diet supplements and gamma irradiation on dissimilation of low-quality roughages by ruminants: III. Effects of feeding gamma-irradiated base diets of wheaten straw and rice straw to sheep

1972 ◽  
Vol 79 (1) ◽  
pp. 55-66 ◽  
Author(s):  
W. R. McManus ◽  
L. Manta ◽  
J. D. McFarlane ◽  
A. C. Gray
Keyword(s):  
Gamma Irradiation ◽  
Wheat Straw ◽  
Rice Straw ◽  
Feed Intake ◽  
Retention Time ◽  
Dry Matter ◽  
Mean Retention Time ◽  
Dry Matter Digestibility ◽  
Voluntary Feed Intake ◽  
Gamma Irradiated

SUMMARYResults of two experiments in which gamma-irradiated low-quality cereal straws were fed ad libitum to sheep, with and without supplements, are reported.In Exp. I 12 adult Merino wethers were fed chopped wheaten straw irradiated at 50 Mrad or non-irradiated wheat straw, each roughage was fed with and without a supplement of urea, minerals and vitamins. In Exp. II 16 adult Merino wethers were fed diets of chopped rice straw or chopped rice straw irradiated at 25, 50 or 75 Mrad; each of these roughages was fed with and without a supplement of casein, minerals and vitamins.Gamma-irradiated diets were invariably associated with reduced mean retention times of undigested feed particles in the digestive tract, and a depressed apparent digestibility of dietary dry matter. Voluntary feed intake of sheep given unsupplemented wheat straw irradiated at 50 Mrad was not significantly affected; nor was the voluntary feed intake of sheep fed unsupplemented irradiated rice straw. Irradiation depressed apparent dry-matter digestibility of unsupplemented wheat straw by 5·2 percentage units; irradiated rice straw diets were depressed by 20·2 (25 Mrad); 9·0 (50 Mrad) and 27·2 (75 Mrad) percentage units compared to non-irradiated diets.Supplementation of irradiated wheat-straw diets tended to restore them to a nutrient worth approximating their original levels in terms of digestibility and intake. Supplementation of rice-straw diets increased voluntary feed intake, because mean retention time was reduced, but diet dry-matter digestibility remained depressed. Supplementation always increased the animals’ state of nitrogen balance; irradiation per se had little effect.Distribution of gastro-intestinal-tract dry-matter content showed a strong tendency for a greater proportion of the ingesta to be located in the hind gut of animals fed irradiated rice-straw diets, thus confirming the enhanced rate of passage of these diets through the tract as determined by the use of dual stained feed-particles. For sheep fed wheat straw the average mean retention time for non-irradiated particles was 50·9 % greater than for irradiated particles. For rice straw diets non-irradiated feedparticles had mean retention time (pooled across supplementation and irradiation treatments) 30·8% greater than for irradiated particles.Distinct changes occurred in the ratio of acetic to propionic acid in the rumen liquor of sheep fed irradiated diets which are suggestive of an alteration in species composition of the foregut microbial population.Evidence is presented for the presence in irradiated diets of a factor or factors toxic to the ruminal microbiota.Irradiation at the levels used decreased feed particle size, but any advantage so obtained was offset by an enhanced rate of passage of feedstuff through the tract with consequent depression in digestibility. It is concluded that further work is required to elucidate the effects reported and to further establish optimum conditions for use of gamma irradiation as a technique in the treatment of low-quality roughages for use by ruminants.

The role of silicon in forages: A quantitative X-Ray study

1987 ◽  
Vol 45 ◽  
pp. 416-417
Author(s):  
Janet H. Woodward ◽  
D. E. Akin
Keyword(s):  
Sodium Acetate ◽  
Dry Matter ◽  
Acetate Buffer ◽  
Plant Tissues ◽  
Sodium Acetate Buffer ◽  
X Ray ◽  
Dry Matter Digestibility ◽  
Percent Composition

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.

Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

2020 ◽  
pp. 1-9
Author(s):  
Pınar Ercan ◽  
Sedef Nehir El
Keyword(s):  
Inhibitory Activity ◽  
Digestive Enzymes ◽  
Positive Control ◽  
Anthocyanin Content ◽  
Inhibitory Effects ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Before And After ◽  
Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

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