scholarly journals Platelet Rich Concentrate Promotes Early Cellular Proliferation and Multiple Lineage Differentiation of Human Mesenchymal Stromal CellsIn Vitro

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Samuel Shani ◽  
Raja Elina Ahmad ◽  
Sangeetha Vasudevaraj Naveen ◽  
Malliga Raman Murali ◽  
Karunanithi Puvanan ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Cellular Proliferation ◽  
Expression Study ◽  
Early Differentiation ◽  
Proliferative Effect ◽  
Lineage Differentiation ◽  
Significant Difference ◽  
Time Point

Platelet rich concentrate (PRC) is a natural adjuvant that aids in human mesenchymal stromal cell (hMSC) proliferationin vitro; however, its role requires further exploration. This study was conducted to determine the optimal concentration of PRC required for achieving the maximal proliferation, and the need for activating the platelets to achieve this effect, and if PRC could independently induce early differentiation of hMSC. The gene expression of markers for osteocytes (ALP, RUNX2), chondrocytes (SOX9, COL2A1), and adipocytes (PPAR-γ) was determined at each time point in hMSC treated with 15% activated and nonactivated PRC since maximal proliferative effect was achieved at this concentration. The isolated PRC had approximately fourfold higher platelet count than whole blood. There was no significant difference in hMSC proliferation between the activated and nonactivated PRC. Only RUNX2 and SOX9 genes were upregulated throughout the 8 days. However, protein expression study showed formation of oil globules from day 4, significant increase in ALP at days 6 and 8(P≤0.05), and increased glycosaminoglycan levels at all time points(P<0.05), suggesting the early differentiation of hMSC into osteogenic and adipogenic lineages. This study demonstrates that the use of PRC increased hMSC proliferation and induced early differentiation of hMSC into multiple mesenchymal lineages, without preactivation or addition of differentiation medium.

Antenatal Mesenchymal Stromal Cell Extracellular Vesicle Treatment Preserves Lung Development in a Model of Bronchopulmonary Dysplasia Due to Chorioamnionitis

2021 ◽  
Author(s):  
Alison Nicole Abele ◽  
Elizabeth S Taglauer ◽  
Maricar Almeda ◽  
Noah Wilson ◽  
Abigail Abikoye ◽  
...  
Keyword(s):  
Bronchopulmonary Dysplasia ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Lung Mechanics ◽  
Spiral Artery ◽  
Lung Growth ◽  
Pulmonary Vessel ◽  
Cesarean Section Delivery ◽  
Distal Lung

Background: Antenatal stressors such as chorioamnionitis (CA) increase the risk for bronchopulmonary dysplasia (BPD). Studies have shown that experimental BPD can be ameliorated by postnatal treatment with mesenchymal stromal cell-derived extracellular vesicles (MEx). However, the antenatal efficacy of MEx to prevent BPD is unknown. Objective: To determine whether antenatal MEx therapy attenuates intrauterine inflammation and preserves lung growth in a rat model of CA-induced BPD. Methods: At embryonic day (E)20, rat litters were treated with intra-amniotic injections of saline, endotoxin (ETX) to model chorioamnionitis, MEx, or ETX plus MEx followed by cesarean section delivery with placental harvest at E22. Placental and lung evaluations were conducted at day 0 and day 14, respectively. To assess the effects of ETX and MEx on lung growth in vitro, E15 lung explants were imaged for distal branching. Results: Placental tissues from ETX-exposed pregnancies showed increased expression of inflammatory markers NLRP-3 and IL-1ß and altered spiral artery morphology. Additionally, infant rats exposed to intrauterine ETX had reduced alveolarization and pulmonary vessel density (PVD), increased right ventricular hypertrophy (RVH), and decreased lung mechanics. Intrauterine MEx therapy of ETX-exposed pups reduced inflammatory cytokines, normalized spiral artery architecture, and preserved distal lung growth and mechanics. In vitro studies showed that MEx treatment enhanced distal lung branching and increased VEGF and SPC gene expression. Conclusions: Antenatal MEx treatment preserved distal lung growth and reduced intrauterine inflammation in a model of CA-induced BPD. We speculate that MEx may provide a novel therapeutic strategy to prevent BPD due to antenatal inflammation.

scholarly journals Enriched osteogenic potential of CD317-negative mesenchymal stromal cell populations in vitro and in vivo

Bone Reports ◽  
2020 ◽  
Vol 13 ◽  
pp. 100509
Author(s):  
Alasdair G. Kay ◽  
James Fox ◽  
Andrew Stone ◽  
Sally James ◽  
Elizabeth Kapasa ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Osteogenic Potential ◽  
Cell Populations

139 EFFICIENT CONDITIONS OF CYTOPLASMIC MICROINJECTION OF FOREIGN DNA TO GENERATE PORCINE TRANSGENIC EMBRYOS

2015 ◽  
Vol 27 (1) ◽  
pp. 162
Author(s):  
D. B. O. Malaweera ◽  
G. Y. Kim ◽  
S. Ramachandra ◽  
J. Y. Jung ◽  
Y. W. Lee ◽  
...  
Keyword(s):  
Time Development ◽  
Injection Time ◽  
Gfp Expression ◽  
Development Rates ◽  
Significant Difference ◽  
Optimal Injection ◽  
Foreign Dna ◽  
Transgenic Embryos ◽  
Time Point

To establish the efficient cytoplasmic microinjection system in the porcine embryos, pEGFP-N1 plasmid were microinjected into porcine parthenogenetic (PA) and in vitro-fertilized (IVF) embryos to investigate the optimal injection time, volume, and concentration. In experiment 1, to investigate the optimal injection time, development rates were compared among groups of 4 different time durations (2, 4, 6, and 8 hours) in the PA and IVF embryos with time point after activation and sperm removal, respectively. There were no significant differences (P < 0.05) between the 4 groups regarding the cleavage rates. However, there were significant differences (P < 0.05) in development to the blastocysts (4.4, 8.9, 3.9, 0.6%) and GFP expression in blastocysts (1.3, 5.7, 2.3, 0.0%), which was injected after postactivation of 4 hours compared with another 3 groups. The IVF embryos injected after 2 and 4 hours expressed GFP significantly higher than the other two groups, which injected at 6 and 8 hours (P < 0.05). In experiment 2, EGFP-N1 with 2 different concentrations (20 and 50 ng μL–1) was injected in the PA and IVF embryos to investigate the optimal concentration. In PA embryos, there were significant differences in 20 ng μL–1-injected embryos, which had higher cleavage (58.8 v. 41.9%) than blastocysts (13.0 v. 11.1%) and GFP expression rates (P < 0.05). In IVF embryos, GFP were expressed only in 20 ng μL–1 embryos, GFP (4.2%) in the blastocysts showed no significant difference in the cleavage (77.3 v. 64.7%) and blastocyst rates (26.4 v. 23.5%). In experiment 3, three different volumes (5, 10, and 20 pL) were microinjected into porcine embryos to determine the most appropriate volume. Out of 3 groups, significantly higher development rates of cleavage (68.3, 58.0, 29.3%), blastocysts (11.7, 12.7, 0.5%), and GFP-expressed blastocysts (2.9, 7.8, 0.0%) were shown in the 10-pL group (P < 0.05). In conclusion, these results imply that a 20 ng μL–1 concentration, 10 pL of volume, injection 4 hours after activation for PA embryos, as well as injection 2 and 4 hours after sperm removal, a 20 ng μL–1 concentration, and 10 pL of volume for IVF embryos were more effective cytoplasmic microinjection conditions.

Immortalized human fetal bone marrow-derived mesenchymal stromal cell expressing suicide gene for anti-tumor therapy in vitro and in vivo

Cytotherapy ◽  
2013 ◽  
Vol 15 (12) ◽  
pp. 1484-1497 ◽  
Author(s):  
Wayne Y.W. Lee ◽  
Ting Zhang ◽  
Carol P.Y. Lau ◽  
C.C. Wang ◽  
Kai-Ming Chan ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Tumor Therapy ◽  
Suicide Gene ◽  
Fetal Bone
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