scholarly journals The Tissue Response and Degradation of Electrospun Poly(ε-caprolactone)/Poly(trimethylene-carbonate) Scaffold in Subcutaneous Space of Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Tao Jiang ◽  
Guoquan Zhang ◽  
Wentong He ◽  
Hui Li ◽  
Xun Jin
Keyword(s):  
Tissue Regeneration ◽  
Foreign Body Reaction ◽  
Vascular Tissue ◽  
Tissue Response ◽  
Trimethylene Carbonate ◽  
Subcutaneous Implantation ◽  
Degradation Rates ◽  
Subcutaneous Space ◽  
Blend Ratios

Due to the advantage of controllability on the mechanical property and the degradation rates, electrospun PCL/PTMC nanofibrous scaffold could be appropriate for vascular tissue engineering. However, the tissue response and degradation of electrospun PCL/PTMC scaffold in vivo have never been evaluated in detail. So, electrospun PCL/PTMC scaffolds with different blend ratios were prepared in this study. Mice subcutaneous implantation showed that the continuous degradation of PCL/PTMC scaffolds induced a lasted macrophage-mediated foreign body reaction, which could be in favor of the tissue regeneration in graft.

In vivo behavior of trimethylene carbonate and ε-caprolactone-based (co)polymer networks: Degradation and tissue response

2010 ◽  
Vol 95A (3) ◽  
pp. 940-949 ◽  
Author(s):  
Erhan Bat ◽  
Josée A. Plantinga ◽  
Martin C. Harmsen ◽  
Marja J. A. van Luyn ◽  
Jan Feijen ◽  
...  
Keyword(s):  
Polymer Networks ◽  
Tissue Response ◽  
Trimethylene Carbonate

Preparation of Electrospun Poly(ε-caprolactone)/Poly(trimethylene carbonate) Blend Scaffold for In Situ Vascular Tissue Engineering

2012 ◽  
Vol 629 ◽  
pp. 60-63
Author(s):  
Tao Jiang ◽  
Guo Quan Zhang ◽  
Hui Li ◽  
Ji Na Xun
Keyword(s):  
Tissue Engineering ◽  
Vascular Graft ◽  
Vascular Tissue ◽  
Electrospinning Process ◽  
Vascular Tissue Engineering ◽  
Trimethylene Carbonate ◽  
Scaffold Materials ◽  
Degradation Time

In the active field of vascular graft research, in situ vascular tissue engineering is a novel concept. This approach aims to use biodegradable synthetic materials. After implantation, the synthetic material progressively degrades and should be replaced by autologous cells. Poly (ε-caprolactone) (PCL) is often used for vascular graft because of its good mechanical strength and its biocompatibility. It is easily processed into micro and nano-fibers by electrospinning to form a porous, cell-friendly scaffold. However, the degradation time of polycaprolactone is too long to match the tissue regeneration time. In this study, poly (ε-caprolactone) /poly (trimethylene carbonate) (PTMC) blend scaffold materials have been prepared for biodegradable vascular graft using an electrospinning process. Because the degradation time of PTMC is shorter than PCL in vivo. The morphological characters of PCL/PTMC blend scaffold materials were investigated by scanning electron microscope (SEM). The molecular components and some physical characteristics of the blend scaffold materials were tested by FT-IR and DSC analysis.

In vivo behavior of poly(1,3-trimethylene carbonate) and copolymers of 1,3-trimethylene carbonate withD,L-lactide or ε-caprolactone: Degradation and tissue response

2003 ◽  
Vol 67A (3) ◽  
pp. 1044-1054 ◽  
Author(s):  
A. P. Pêgo ◽  
M. J. A. Van Luyn ◽  
L. A. Brouwer ◽  
P. B. van Wachem ◽  
A. A. Poot ◽  
...  
Keyword(s):  
Tissue Response ◽  
Trimethylene Carbonate

scholarly journals Physicochemical and Tissue Response of PLA Nanofiber Scaffolds Sterilized by Different Techniques

2018 ◽  
pp. 169-180
Author(s):  
Isarai Mendieta-Barrañon DDS ◽  
Osmar A. Chanes-Cuevas DDS, MSc ◽  
Marco A. Álvarez-Pérez PhD ◽  
Patricia González-Alva DDS, PhD ◽  
Luis A. Medina PhD ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Physicochemical Characterization ◽  
Biochemical Properties ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Poly Lactic Acid ◽  
Response Analysis ◽  
Sterile Product

In recent years, tissue engineering has evolved considerably, due to the problems in the biomedical area concerning tissue regeneration therapies. Currently, work has been focused on the synthesis and physicochemical characterization of poly lactic acid scaffolds, a synthetic polyester that has been extensively study for its excellent biocompatibility and biodegradability. Moreover, sterilization strategies of scaffold are a crucial step for its application in tissue regeneration, however, the sterilization process have to maintain the structural and biochemical properties of the scaffold. Therefore, it is very important to carry out studies on the sterilization methods of the sample’s material, since translational medicine is intended for in vivo applications. The aim of the present study was designed to analyze the effects of different sterilization techniques, i.e. ethylene oxide (ETO), gamma radiation (GR) and hydrogen peroxide-based plasma (H2O2) in biodegradable PLA scaffolds, and to determine the best sterilization technique to render a sterile product with minimal degradation and deformation, and good tissue response. Analysis of surface morphology showed that ETO and GR modified the PLA scaffolds without any change in its chemical composition. Moreover, the histological response showed that the scaffolds are biocompatible and those sterilized by GR showed a more severe inflammatory response, accompanied with the presence of giant foreign body cells. In conclusion, the results show that among sterilization techniques used in the preset study, the best results were observed with H2O2 sterilization, since it did not significantly modify the surface structure of the PLA fibers and their in vivo response did not cause an unfavorable tissue reaction.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

Surface Modification by Nanobiomaterials for Vascular Tissue Engineering Applications

2020 ◽  
Vol 27 (10) ◽  
pp. 1634-1646 ◽  
Author(s):  
Huey-Shan Hung ◽  
Shan-hui Hsu
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Thrombus Formation ◽  
Vascular Grafts ◽  
Vascular Tissue Engineering ◽  
Great Success ◽  
Natural Polymers ◽  
Vascular Biomaterials

Treatment of cardiovascular disease has achieved great success using artificial implants, particularly synthetic-polymer made grafts. However, thrombus formation and restenosis are the current clinical problems need to be conquered. New biomaterials, modifying the surface of synthetic vascular grafts, have been created to improve long-term patency for the better hemocompatibility. The vascular biomaterials can be fabricated from synthetic or natural polymers for vascular tissue engineering. Stem cells can be seeded by different techniques into tissue-engineered vascular grafts in vitro and implanted in vivo to repair the vascular tissues. To overcome the thrombogenesis and promote the endothelialization effect, vascular biomaterials employing nanotopography are more bio-mimic to the native tissue made and have been engineered by various approaches such as prepared as a simple surface coating on the vascular biomaterials. It has now become an important and interesting field to find novel approaches to better endothelization of vascular biomaterials. In this article, we focus to review the techniques with better potential improving endothelization and summarize for vascular biomaterial application. This review article will enable the development of biomaterials with a high degree of originality, innovative research on novel techniques for surface fabrication for vascular biomaterials application.

Lactoferrin in Bone Tissue Regeneration

2020 ◽  
Vol 27 (6) ◽  
pp. 838-853 ◽  
Author(s):  
Madalina Icriverzi ◽  
Valentina Dinca ◽  
Magdalena Moisei ◽  
Robert W. Evans ◽  
Mihaela Trif ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Cells ◽  
Bone Diseases ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Bone Tissue Regeneration ◽  
Bone Homeostasis ◽  
Active Compounds

: Among the multiple properties exhibited by lactoferrin (Lf), its involvement in bone regeneration processes is of great interest at the present time. A series of in vitro and in vivo studies have revealed the ability of Lf to promote survival, proliferation and differentiation of osteoblast cells and to inhibit bone resorption mediated by osteoclasts. Although the mechanism underlying the action of Lf in bone cells is still not fully elucidated, it has been shown that its mode of action leading to the survival of osteoblasts is complemented by its mitogenic effect. Activation of several signalling pathways and gene expression, in an LRPdependent or independent manner, has been identified. Unlike the effects on osteoblasts, the action on osteoclasts is different, with Lf leading to a total arrest of osteoclastogenesis. : Due to the positive effect of Lf on osteoblasts, the potential use of Lf alone or in combination with different biologically active compounds in bone tissue regeneration and the treatment of bone diseases is of great interest. Since the bioavailability of Lf in vivo is poor, a nanotechnology- based strategy to improve the biological properties of Lf was developed. The investigated formulations include incorporation of Lf into collagen membranes, gelatin hydrogel, liposomes, loading onto nanofibers, porous microspheres, or coating onto silica/titan based implants. Lf has also been coupled with other biologically active compounds such as biomimetic hydroxyapatite, in order to improve the efficacy of biomaterials used in the regulation of bone homeostasis. : This review aims to provide an up-to-date review of research on the involvement of Lf in bone growth and healing and on its use as a potential therapeutic factor in bone tissue regeneration.

scholarly journals Effective decellularisation of human saphenous veins for biocompatible arterial tissue engineering applications: Bench optimisation and feasibility in vivo testing

2021 ◽  
Vol 12 ◽  
pp. 204173142098752
Author(s):  
Nadiah S Sulaiman ◽  
Andrew R Bond ◽  
Vito D Bruno ◽  
John Joseph ◽  
Jason L Johnson ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Strength ◽  
Vascular Tissue ◽  
Sodium Dodecyl ◽  
Host Cells ◽  
Replacement Model ◽  
In Vivo Testing ◽  
Vascular Scaffolds

Human saphenous vein (hSV) and synthetic grafts are commonly used conduits in vascular grafting, despite high failure rates. Decellularising hSVs (D-hSVs) to produce vascular scaffolds might be an effective alternative. We assessed the effectiveness of a detergent-based method using 0% to 1% sodium dodecyl sulphate (SDS) to decellularise hSV. Decellularisation effectiveness was measured in vitro by nuclear counting, DNA content, residual cell viability, extracellular matrix integrity and mechanical strength. Cytotoxicity was assessed on human and porcine cells. The most effective SDS concentration was used to prepare D-hSV grafts that underwent preliminary in vivo testing using a porcine carotid artery replacement model. Effective decellularisation was achieved with 0.01% SDS, and D-hSVs were biocompatible after seeding. In vivo xeno-transplantation confirmed excellent mechanical strength and biocompatibility with recruitment of host cells without mechanical failure, and a 50% patency rate at 4-weeks. We have developed a simple biocompatible methodology to effectively decellularise hSVs. This could enhance vascular tissue engineering toward future clinical applications.

scholarly journals Nanoscale Strontium-Substituted Hydroxyapatite Pastes and Gels for Bone Tissue Regeneration

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1611
Author(s):  
Caroline J. Harrison ◽  
Paul V. Hatton ◽  
Piergiorgio Gentile ◽  
Cheryl A. Miller
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Culture Media ◽  
Full Range ◽  
Sol Gel ◽  
Tissue Growth ◽  
Bone Tissue Regeneration ◽  
Tissue Culture Media ◽  
Bone Deposition

Injectable nanoscale hydroxyapatite (nHA) systems are highly promising biomaterials to address clinical needs in bone tissue regeneration, due to their excellent biocompatibility, bioinspired nature, and ability to be delivered in a minimally invasive manner. Bulk strontium-substituted hydroxyapatite (SrHA) is reported to encourage bone tissue growth by stimulating bone deposition and reducing bone resorption, but there are no detailed reports describing the preparation of a systematic substitution up to 100% at the nanoscale. The aim of this work was therefore to fabricate systematic series (0–100 atomic% Sr) of SrHA pastes and gels using two different rapid-mixing methodological approaches, wet precipitation and sol-gel. The full range of nanoscale SrHA materials were successfully prepared using both methods, with a measured substitution very close to the calculated amounts. As anticipated, the SrHA samples showed increased radiopacity, a beneficial property to aid in vivo or clinical monitoring of the material in situ over time. For indirect methods, the greatest cell viabilities were observed for the 100% substituted SrHA paste and gel, while direct viability results were most likely influenced by material disaggregation in the tissue culture media. It was concluded that nanoscale SrHAs were superior biomaterials for applications in bone surgery, due to increased radiopacity and improved biocompatibility.

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