scholarly journals Maternal Plasma and Amniotic Fluid Chemokines Screening in Fetal Down Syndrome

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Piotr Laudanski ◽  
Monika Zbucka-Kretowska ◽  
Karol Charkiewicz ◽  
Slawomir Wolczynski ◽  
Daniela Wojcik ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Amniotic Fluid ◽  
Blood Plasma ◽  
Simultaneous Determination ◽  
Biochemical Markers ◽  
Chromosomal Abnormalities ◽  
Inflammatory Responses ◽  
Maternal Plasma ◽  
Protein Macroarray

Objective. Chemokines exert different inflammatory responses which can potentially be related to certain fetal chromosomal abnormalities. The aim of the study was to determine the concentration of selected chemokines in plasma and amniotic fluid of women with fetal Down syndrome.Method. Out of 171 amniocentesis, we had 7 patients with confirmed fetal Down syndrome (15th–18th weeks of gestation). For the purpose of our control, we chose 14 women without confirmed chromosomal aberration. To assess the concentration of chemokines in the blood plasma and amniotic fluid, we used a protein macroarray, which allows the simultaneous determination of 40 chemokines per sample.Results. We showed significant decrease in the concentration of 4 chemokines, HCC-4, IL-28A, IL-31, and MCP-2, and increase in the concentration of CXCL7 (NAP-2) in plasma of women with fetal Down syndrome. Furthermore, we showed decrease in concentration of 3 chemokines, ITAC, MCP-3, MIF, and increase in concentration of 4 chemokines, IP-10, MPIF-1, CXCL7, and 6Ckine, in amniotic fluid of women with fetal Down syndrome.Conclusion. On the basis of our findings, our hypothesis is that the chemokines may play role in the pathogenesis of Down syndrome. Defining their potential as biochemical markers of Down syndrome requires further investigation on larger group of patients.

scholarly journals Brief Communication: Maternal Plasma Autoantibodies Screening in Fetal Down Syndrome

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Karol Charkiewicz ◽  
Monika Zbucka-Kretowska ◽  
Joanna Goscik ◽  
Slawomir Wolczynski ◽  
Adam Lemancewicz ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Biochemical Markers ◽  
Cross Validation ◽  
Chromosomal Abnormalities ◽  
Protein Microarray ◽  
Maternal Plasma ◽  
Second Step ◽  
Study Group ◽  
Specificity And Sensitivity

Imbalance in the metabolites levels which can potentially be related to certain fetal chromosomal abnormalities can stimulate mother’s immune response to produce autoantibodies directed against proteins. The aim of the study was to determine the concentration of 9000 autoantibodies in maternal plasma to detect fetal Down syndrome.Method.We performed 190 amniocenteses and found 10 patients with confirmed fetal Down syndrome (15th–18th weeks of gestation). For the purpose of our control we chose 11 women without confirmed chromosomal aberration. To assess the expression of autoantibodies in the blood plasma, we used a protein microarray, which allows for simultaneous determination of 9000 proteins per sample.Results.We revealed 213 statistically significant autoantibodies, whose expression decreased or increased in the study group with fetal Down syndrome. The second step was to create a classifier of Down syndrome pregnancy, which includes 14 antibodies. The predictive value of the classifier (specificity and sensitivity) is 100%, classification errors, 0%, cross-validation errors, 0%.Conclusion.Our findings suggest that the autoantibodies may play a role in the pathophysiology of Down syndrome pregnancy. Defining their potential as biochemical markers of Down syndrome pregnancy requires further investigation on larger group of patients.

Simultaneous analysis of bisphenol A fractions in maternal and fetal compartments in early second trimester of pregnancy

2019 ◽  
Vol 47 (7) ◽  
pp. 765-770 ◽  
Author(s):  
Monika Zbucka-Krętowska ◽  
Urszula Łazarek ◽  
Wojciech Miltyk ◽  
Iwona Sidorkiewicz ◽  
Piotr Pierzyński ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Amniotic Fluid ◽  
Pregnant Women ◽  
Chromosomal Abnormalities ◽  
Maternal Plasma ◽  
Environmental Pollutant ◽  
Maternal Exposure ◽  
Gas Chromatography Mass Spectrometry ◽  
Permeability Factor ◽  
The Impact

Abstract Background Bisphenol A (BPA) is an estrogenic, endocrine-disrupting compound widely used in the industry. It is also a ubiquitous environmental pollutant. Its presence was confirmed in human fetuses, which results from maternal exposure during pregnancy. The mechanisms behind maternal-fetal transfer, and relationships between pregnant women and fetal exposures remain unclear. The aim of this study was to assess the impact of maternal exposure to BPA on the exposure of the fetus. Methods Maternal plasma and amniotic fluid samples were collected from 52 pregnant women undergoing amniocentesis for prenatal diagnosis of chromosomal abnormalities. BPA was measured by gas chromatography-mass spectrometry (GC-MS). The permeability factor – a ratio of fetal-to-maternal BPA concentration – was used as a measure delineating the transplacental transfer of BPA. Results The median concentration of maternal plasma BPA was 8 times higher than the total BPA concentration in the amniotic fluid (8.69 ng/mL, range: 4.3 ng/mL–55.3 ng/mL vs. median 1.03 ng/mL, range: 0.3 ng/mL–10.1 ng/mL). There was no direct relationship between the levels of BPA in maternal plasma and amniotic fluid levels. The permeability factor, in turn, negatively correlated with fetal development (birth weight) (R = −0.54, P < 0.001). Conclusion Our results suggest that the risk of fetal BPA exposure depends on placental BPA permeability rather than the levels of maternal BPA plasma concentration and support general recommendations to become aware and avoid BPA-containing products.

scholarly journals Possibilities for prediction of congenital fetal malformations and chromosomal abnormalities based on the determination of the levels of free amino acids and their nitrogen-containing derivatives in blood plasma of pregnant women

2020 ◽  
pp. 152-158
Author(s):  
L. N. Keda ◽  
A. V. Naumov ◽  
V. YU. Smirnov
Keyword(s):  
Amino Acids ◽  
Blood Plasma ◽  
Pregnant Women ◽  
Free Amino Acids ◽  
Free Amino ◽  
Chromosomal Abnormalities ◽  
Group I ◽  
Fetal Malformations ◽  
In Blood Plasma

Objective: to determine the possibilities for prediction and diagnosis of congenital fetal malformations and chromosomal abnormalities on the basis of the study of the levels of amino acids and their nitrogen-containing derivatives in blood plasma of pregnant women with pathological conditions requiring artificial termination of pregnancy. Material and methods. The content of free amino acids and their nitrogen-containing derivatives was studied in 104 pregnant women having congenital malformations and chromosomal abnormalities in their fetuses at 13-22 weeks` gestation (group I) and 25 women with physiological pregnancy (group II). The amino acid level was determined by the high-performance liquid chromatography method. Results. The levels of 14 out of the 26 studied amino acids in the blood plasma of the pregnant women of group I were statistically higher than those of the women in group II. ROC analysis was used to determine six amino acids (glycine, α-aminobutyric acid, hydroxylysine, glutamic acid, citrulline, serine) and their threshold values which with high accuracy (85.3 %) allow of predicting congenital fetal malformations and chromosomal abnormalities. A prognostic model making it possible to determine high probability of congenital fetal anomalies based on the determination of the concentration of 5-hydroxytryptophan, glycine, asparagine, and serine in blood plasma of pregnant women has been developed. Conclusion. The study of the levels of amino acids and their nitrogen-containing derivatives in plasma of pregnant women at 13-22 weeks` gestation can be used for prenatal diagnosis of congenital fetal malformations and chromosomal abnormalities, as well as used as an additional criterion for making the decision on the necessity for artificial termination of pregnancy upon fetal medical indications.

Simultaneous determination of phosphatidylglycerol and the lecithin/sphingomyelin ratio in amniotic fluid.

1978 ◽  
Vol 24 (7) ◽  
pp. 1144-1146 ◽  
Author(s):  
G R Gotelli ◽  
R E Stanfill ◽  
P M Kabra ◽  
F A Farina ◽  
L J Marton
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Simultaneous Determination ◽  
Chromatographic Method ◽  
White Background ◽  
Molybdenum Blue ◽  
Spray Reagent ◽  
Single Dimension

Abstract We describe a single-dimension thin-layer chromatographic method by which one can simultaneously determine the lecithin/sphingomyelin ratio and the proportion of phosphatidylglycerol in amniotic fluid. The phospholipids are conveniently detected by an improved molybdenum-blue spray reagent, which immediately produces blue spots on a white background. The stained phospholipids are stable for at least 90 min after spraying, and densitometry results in symmetrical peaks that are easily quantitated.

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