scholarly journals Evaluation ofIn VitroAntimalarial Activity of Different Extracts ofArtemisia aucheriBoiss. andA. armeniacaLam. and Fractions of the Most Potent Extracts

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Mahdi Mojarrab ◽  
Ali Shiravand ◽  
Abbas Delazar ◽  
Fariba Heshmati Afshar
Keyword(s):  
Liquid Chromatography ◽  
Silica Gel ◽  
Antimalarial Activity ◽  
Solvent Mixtures ◽  
Vacuum Liquid Chromatography

Ten extracts with different polarity from two IranianArtemisiaspecies,A. armeniacaLam. andA. aucheriBoiss, were screened for their antimalarial properties byin vitro  β-hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC50values of 1.36 ± 0.01 and 1.83 ± 0.03 mg/mL and IC90values of 2.12 ± 0.04 and 2.62 ± 0.09 mg/mL forA. armeniacaandA. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract ofA. armeniacaand four fractions from DCM extract ofA. aucherishowed potent antimalarial activity with reducing IC50and IC90values compared to extracts. The most potent fraction belonged to DCM extract ofA. armeniacawith IC50and IC90values of 0.47 ± 0.006 and 0.71 ± 0.006 mg/mL, respectively.

scholarly journals Aktivitas Antimalaria Ekstrak Kasar Etanol dan Fraksi n-Heksana Rumput Bambu (Lophatherum gracile B.) secara in Vitro

ALCHEMY ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 18
Author(s):  
Ella Wulandari ◽  
Dewi Yuliani ◽  
Elok Kamilah Hayati ◽  
Roihatul Muti'ah
Keyword(s):  
Mass Spectrometry ◽  
Plasmodium Falciparum ◽  
Liquid Chromatography ◽  
Antimalarial Activity ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Liquid Chromatography Mass Spectrometry ◽  
Mosquito Bite

<p>Malaria is a disease caused by infectious parasite <em>Plasmodium falciparum</em> and can be transmitted through mosquito bite. The aim of this research was to study antimalarial activity <em>in vitro</em> on crude ethanol extract and n-hexane fraction of bamboo grass (<em>Lophatherum gracile</em> B.). Extraction was carried out by ethanol 80% solvent and fractionation was conducted by n-hexane. Determination of antimalarial activity was subjected to <em>P. falciparum</em> strain 3D7. According to phytochemical test, crude ethanol extract contained tannin and terpenoid, whilst n-hexane fraction contained tannin and steroid. The capability of crude ethanol extract and n-hexane fraction to inhibit <em>P. falciparum</em> was represented by IC<sub>50</sub> value. The value of both samples respectively was 12.49 and 61.49 µg/mL. Identification based on LC-MS (liquid chromatography-mass spectrometry), n-hexane fraction shown the presence of tannin and steroid compounds.</p><p>  </p><p>Malaria merupakan penyakit yang disebabkan oleh infeksi parasit <em>Plasmodium falciparum</em> yang dapat ditularkan melalui gigitan nyamuk. Penelitian ini bertujuan untuk mengetahui aktivitas antimalaria secara <em>in vitro</em> pada ekstrak kasar etanol dan fraksi n-heksana rumput bambu (<em>L</em><em>ophat</em><em>h</em><em>erum gracile </em>B.). Proses ekstraksi dilakukan dengan pelarut etanol 80% dan fraksinasi dengan n-heksana. Uji aktivitas antimalaria dilakukan pada parasit <em>P. falciparum </em><em>strain</em> 3D7<em>.</em> Hasil uji fitokimia menunjukkan ekstrak etanol mengandung tanin dan terpenoid, sedangkan fraksi n-heksana mengandung tanin dan steroid. Kemampuan ekstrak etanol dan fraksi n-heksana dalam menghambat parasit <em>P. falciparum</em> menghasilkan nilai IC<sub>50</sub> masing-masing sebesar 12,49 dan 61,49 µg/mL. Identifikasi senyawa dengan KC-SM (kromatografi cair-spektrometri massa) pada fraksi n-heksana menunjukkan adanya senyawa tanin dan steroid.</p>

scholarly journals AKTIVITAS ANTIBAKTERI FRAKSI SAPONIN DARI KULIT BATANG TUMBUHAN KASTURI (Mangifera casturi)

BIOSCIENTIAE ◽  
2018 ◽  
Vol 7 (2) ◽  
Author(s):  
K. Rosyidah ◽  
S. A. Nurmuhaimina ◽  
N. Komari ◽  
M. D. Astuti
Keyword(s):  
Antibacterial Activity ◽  
Liquid Chromatography ◽  
Silica Gel ◽  
Methanol Extract ◽  
Stem Bark ◽  
E Coli ◽  
Vacuum Liquid Chromatography

This research to study antibacterial activity (E.coli and S.aureus) of saponin fractions from stem bark of Mangifera casturi. Powdered of stem bark of M.casturi (512,67 g) were extracted with methanol. Methanol extract (32.57 g) was partitionated with n-hexane to give saponin extract. Saponin extract (5.98 g) was partitionated by Vacuum Liquid Chromatography (VLC) over silica gel to give 39 fractions were grouped into four major fractions A-D by combining fractions with similar TLC profile. Every fractions studied antibacterial activity with E. coli and S. Aureus with difusi methode. Fraction A most active then other fraction with inhibition diameter 10.3 ± 0.5 mm (E.coli) and 10.8 ± 0.3 mm (S.aureus).

scholarly journals Population Pharmacokinetics of Artemether, Lumefantrine, and Their Respective Metabolites in Papua New Guinean Children with Uncomplicated Malaria

2011 ◽  
Vol 55 (11) ◽  
pp. 5306-5313 ◽  
Author(s):  
Sam Salman ◽  
Madhu Page-Sharp ◽  
Susan Griffin ◽  
Kaye Kose ◽  
Peter M. Siba ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Uncomplicated Malaria ◽  
Antimalarial Activity ◽  
Parent Drug ◽  
Published Data ◽  
Active Metabolites ◽  
Time Curve ◽  
Limit Of Quantitation ◽  
Papua New Guinean

ABSTRACTThere are sparse published data relating to the pharmacokinetic properties of artemether, lumefantrine, and their active metabolites in children, especially desbutyl-lumefantrine. We studied 13 Papua New Guinean children aged 5 to 10 years with uncomplicated malaria who received the six recommended doses of artemether (1.7 mg/kg of body weight) plus lumefantrine (10 mg/kg), given with fat over 3 days. Intensive blood sampling was carried out over 42 days. Plasma artemether, dihydroartemisinin, lumefantrine, and desbutyl-lumefantrine were assayed using liquid chromatography-mass spectrometry or high-performance liquid chromatography. Multicompartmental pharmacokinetic models for a drug plus its metabolite were developed using a population approach that included plasma artemether and dihydroartemisinin concentrations below the limit of quantitation. Although artemether bioavailability was variable and its clearance increased by 67.8% with each dose, the median areas under the plasma concentration-time curve from 0 h to infinity (AUC0-∞s) for artemether and dihydroartemisinin (3,063 and 2,839 μg·h/liter, respectively) were similar to those reported previously in adults with malaria. For lumefantrine, the median AUC0–∞(459,980 μg·h/liter) was also similar to that in adults with malaria. These data support the higher dose recommended for children weighing 15 to 35 kg (35% higher than that for a 50-kg adult) but question the recommendation for a lower dose in children weighing 12.5 to 15 kg. The median desbutyl-lumefantrine/lumefantrine ratio in the children in our study was 1.13%, within the range reported for adults and higher at later time points because of the longer desbutyl-lumefantrine terminal elimination half-life. A combined desbutyl-lumefantrine and lumefantrine AUC0–∞weighted onin vitroantimalarial activity was inversely associated with recurrent parasitemia, suggesting that both the parent drug and the metabolite contribute to the treatment outcome of artemether-lumefantrine.

scholarly journals Determination of Total Phenol and Flavonoid Contents and Antioxidant Activity from Extract Fraction of Sappan Wood (Caesalpinia sappan L.) by Liquid-Liquid Extraction and Vacuum Liquid Chromatography

2021 ◽  
Vol 33 (8) ◽  
pp. 1729-1735
Author(s):  
Masturi ◽  
D. Alighiri ◽  
S.S. Edie ◽  
U. Hanisyifa ◽  
A. Drastisianti
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Liquid Extraction ◽  
Total Phenolic ◽  
Caesalpinia Sappan ◽  
Liquid Liquid Extraction ◽  
Extract Fraction ◽  
Vacuum Liquid Chromatography

This study reported the antioxidant activity of sappan wood (Caesalpinia sappan L.) extract and their extract fraction from the liquid-liquid extraction and vacuum liquid chromatography. The study comprises with phytochemical assessments, evaluation of total phenolic content (TPC) and total flavonoid content (TFC). The evaluation of antioxidant activity was carried out by using the DPPH method. The effectiveness of antioxidant activity in sappan wood compared to quercetin. Extract of sappan wood (ESW) yields of 5.40%, extract fraction from liquid-liquid extraction (FE LLE) yields of 52.15% and extract fraction from vacuum liquid chromatography (FE VLC) with silica gel and eluent sequential from n-hexane, 5% n-hexane/95% ethyl acetate, ethyl acetate and 5% ethyl acetate/45% methanol was produced 60 of fractions extract. The thin-layer chromatography (TLC) was analyzed using silica gel as a stationary phase with the mobile phase of n-hexane:ethyl acetate (1:0.55) was obtained 10 of fractions extract in fractions of 6 and 7, which showed the most positive results. The phytochemical test indicated the presence of phenolic and flavonoids compounds. The highest values for TPC was FE LLE (216.67 mg GAE/g), FE VLC 160.83 (mg GAE/g) and ESW (18.33 mg GAE/g), respectively. The highest values for TFC were F VLC (152.48 mg QE/g), FE LLE (66.2 mg QE/g) and ESW (48.4 mg QE/g), respectively. Then, the highest of antioxidants activity were F VLC (5.42 μg/mL), FE LLE (6.41 μg/mL), quercetin (6.52 μg/mL) and ESW (7.82 μg/mL), respectively. The results showed that the highest of TFC was related to the antioxidant activity. However, the highest value of TPC was not showed the highest antioxidant activity. Both ESW, FE LLE and FE VLC with quercetin as control exhibited powerful antioxidants.

Obtaining and Characterization of Alhagi persarum Boiss. et Buhse Callus Cell Cultures, Isoflavonoid Producers

2020 ◽  
Vol 36 (6) ◽  
pp. 35-48
Author(s):  
D.V. Коchkin ◽  
G.I. Sobolkovа ◽  
А.А. Fоmеnkov ◽  
R.А. Sidorov ◽  
А.М. Nоsоv
Keyword(s):  
Fatty Acids ◽  
Cell Culture ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Cell Cultures ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Gas Liquid Chromatography ◽  
Callus Cell

The physiological characteristics of the callus cell cultures of Alhagi persarum Boiss et Buhse, a member of the legume family, widely used in folk medicine, have been studied. It was shown that the source of the explant was an important factor in the initiation of callusogenesis: more intense callusogenesis (almost 100%) was observed for explants from various organs of sterile seedlings, rather than intact plants (less than 30%). As a result, more than 20 lines of morphologically different callus cell cultures were obtained, and the growth parameters for the 5 most intensively growing lines were determined. The composition of fatty acids (FA) of total lipids and secondary metabolites in the most physiologically stable callus line Aр-207 was analyzed. Using capillary gas-liquid chromatography with mass spectrometric detection (GLC-MS), 19 individual C12--C24 FAs were identified, the main fraction of which were palmitic (~ 23%), stearic (~ 22%), linoleic (~ 14%) and α-linolenic (~ 33%) acids. The established atypical ratio of FAs (a simultaneous high content of both saturated FAs and polyunsaturated α-linolenic acid) is possibly due to the adaptation of cells to in vitro growth conditions. Phytochemical analysis of the secondary metabolites was carried out using ultra-performance liquid chromatography with electrospray ionization mass spectrometric detection (UPLC MS). Compounds belonging to different structural groups of isoflavones were found. Aglycones (calycosin, formononetin and afrormosin isomer), glucosides (formononetin glucoside), as well as esters of glucosides (malonylglycosides of calicosin, formononetin, afrormosin isomers, glycitein and genistein) were detected. These secondary metabolites are widespread in plants of the Fabaceae family; however, isoflavones are rare in representatives of the Alhagi genus. The presence of malonylated isoflavone glycosides in Alhagi spp. was shown for the first time. endemic plant species, Alhagi, in vitro cell culture, callus cell culture, isoflavones, fatty acids All studies were carried out using the equipment of the "Experimental Biotechnological Facility" and the "All-Russian Collection of Cell Cultures of Higher Plants" of IРР RAS. This work was supported by the Russian Foundation for Basic Research (RFBR), contract no.18-54-06021 (Az_a), and the Government of the Russian Federation, Megagrant Project no. 075-15-2019-1882.

Synthesis, Antimalarial Evaluation and SAR Study of Some 1,3,5-Trisubstituted Pyrazoline Derivatives

2019 ◽  
Vol 16 (10) ◽  
pp. 807-817 ◽  
Author(s):  
Shilpy Aggarwal ◽  
Deepika Paliwal ◽  
Dhirender Kaushik ◽  
Girish Kumar Gupta ◽  
Ajay Kumar
Keyword(s):  
Antimalarial Activity ◽  
Analysis Data ◽  
Docking Study ◽  
Maximum Activity ◽  
Elemental Analysis Data ◽  
Mass Spectral ◽  
Structure Activity ◽  
Pharmacokinetic Properties ◽  
Sar Study

The synthesis of a novel series of 1,3,5-trisubstitiuted pyrazoline was achieved by refluxing chalcone derivative with different heteroaryl hydrazines. The newly synthesized compounds were characterized by 1H NMR, 13CNMR, mass spectral and elemental analysis data. The synthetic series of novel pyrazoline hybrids was screened for in vitro schizont maturation assay against chloroquine sensitive 3D7 strain of Plasmodium falciparum. Most of the compounds showed promising in vitro antimalarial activity against CQ sensitive strain. The preliminary structure-activity relationship study showed that quinoline substituted analog at position N-1 showed maximum activity followed by benzothiazole substitution, while phenyl substitution lowers the antimalarial activity. The observed activity was persistent by the docking study on P. falciparum cystein protease falcipain-2. The pharmacokinetic properties were also studied using ADME prediction.

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