scholarly journals Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Marjan Nokhbehsaim ◽  
Sema Keser ◽  
Andressa Vilas Boas Nogueira ◽  
Joni Augusto Cirelli ◽  
Søren Jepsen ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Periodontal Ligament ◽  
Periodontal Regeneration ◽  
In Vitro Study ◽  
Enamel Matrix Derivative ◽  
Periodontal Ligament Cells ◽  
Cell Functions ◽  
Diabetes And Obesity

Type 2 diabetes and obesity are increasing worldwide and linked to periodontitis, a chronic disease which is characterized by the irreversible destruction of the tooth-supporting tissues, that is, periodontium. The mechanisms underlying the association of diabetes mellitus and obesity with periodontal destruction and compromised periodontal healing are not well understood, but decreased plasma levels of adiponectin, as found in diabetic and obese individuals, might be a critical mechanistic link. The aim of this in vitro study was to examine the effects of adiponectin on periodontal ligament (PDL) cells under normal and regenerative conditions, and to study the regulation of adiponectin and its receptors in these cells. Adiponectin stimulated significantly the expression of growth factors and extracellular matrix, proliferation, and in vitro wound healing, reduced significantly the constitutive tumor necrosis factor-αexpression, and caused a significant upregulation of its own expression. The beneficial actions of enamel matrix derivative on a number of PDL cell functions critical for periodontal regeneration were partially enhanced by adiponectin. The periodontopathogenPorphyromonas gingivalisinhibited the adiponectin expression and stimulated the expression of its receptors. In conclusion, reduced levels of adiponectin, as found in type 2 diabetes and obesity, may compromise periodontal health and healing.

Identifying Phenotypically Distinct Fibroblast Subsets in Type 2 Diabetes–Associated Iatrogenic Laryngotracheal Stenosis

2021 ◽  
pp. 019459982110147
Author(s):  
Ioan A. Lina ◽  
Alexandra Berges ◽  
Rafael Ospino ◽  
Ruth J. Davis ◽  
Kevin M. Motz ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Flow Cytometry ◽  
Pearson Correlation ◽  
Phenotypic Expression ◽  
In Vitro Study ◽  
Cell Protein ◽  
Laryngotracheal Stenosis ◽  
Tertiary Referral Center

Objective Iatrogenic laryngotracheal stenosis (iLTS) is the pathologic narrowing of the glottis, subglottis, and/or trachea secondary to intubation or tracheostomy related injury. Patients with type 2 diabetes mellitus (T2DM) are more likely to develop iLTS. To date, the metabolomics and phenotypic expression of cell markers in fibroblasts derived from patients with T2DM and iLTS are largely unknown. Study Design Controlled in vitro cohort study. Setting Tertiary referral center (2017-2020). Methods This in vitro study assessed samples from 6 patients with iLTS who underwent surgery at a single institution. Fibroblasts were isolated from biopsy specimens of laryngotracheal scar and normal-appearing trachea and compared with controls obtained from the trachea of rapid autopsy specimens. Patients with iLTS were subcategorized into those with and without T2DM. Metabolic substrates were identified by mass spectrometry, and cell protein expression was measured by flow cytometry. Results T2DM iLTS-scar fibroblasts had a metabolically distinct profile and clustered tightly on a Pearson correlation heat map as compared with non-T2DM iLTS-scar fibroblasts. Levels of itaconate were elevated in T2DM iLTS-scar fibroblasts. Flow cytometry demonstrated that T2DM iLTS-scar fibroblasts were associated with higher CD90 expression (Thy-1; mean, 95%) when compared with non-T2DM iLTS-scar (mean, 83.6%; P = .0109) or normal tracheal fibroblasts (mean, 81.1%; P = .0042). Conclusions Scar-derived fibroblasts from patients with T2DM and iLTS have a metabolically distinct profile. These fibroblasts are characterized by an increase in itaconate, a metabolite related to immune-induced scar remodeling, and can be identified by elevated expression of CD90 (Thy-1) in vitro.

Potential of the propolis as storage medium to preserve the viability of cultured human periodontal ligament cells: an in vitro study

2011 ◽  
Vol 27 (2) ◽  
pp. 102-108 ◽  
Author(s):  
Payal Saxena ◽  
Vandana Aditya Pant ◽  
Kulvindar Kaur Wadhwani ◽  
Mahendra Pratap Kashyap ◽  
Saurabh Kumar Gupta ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
In Vitro Study ◽  
Vitro Study ◽  
Periodontal Ligament Cells ◽  
Storage Medium ◽  
Human Periodontal Ligament Cells

Evaluation of Rubus grandifolius L. (wild blackberries) activities targeting management of type-2 diabetes and obesity using in vitro models

2019 ◽  
Vol 123 ◽  
pp. 443-452 ◽  
Author(s):  
Vítor Spínola ◽  
Joana Pinto ◽  
Eulogio J. Llorent-Martínez ◽  
Helena Tomás ◽  
Paula C. Castilho
Keyword(s):  
Type 2 Diabetes ◽  
In Vitro Models ◽  
Diabetes And Obesity

A novel small molecule A2A adenosine receptor agonist, indirubin-3′-monoxime, alleviates lipid-induced inflammation and insulin resistance in 3T3-L1 adipocytes

2019 ◽  
Vol 476 (16) ◽  
pp. 2371-2391 ◽  
Author(s):  
Saynaz A. Choudhary ◽  
Nikita Bora ◽  
Dipanjan Banerjee ◽  
Leena Arora ◽  
Anindhya Sundar Das ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Adenosine Receptor ◽  
In Vitro Study ◽  
A2a Adenosine Receptor ◽  
Insulin Resistant ◽  
Adenosine Receptor Agonist ◽  
Inflammatory State

Abstract Saturated free fatty acid-induced adipocyte inflammation plays a pivotal role in implementing insulin resistance and type 2 diabetes. Recent reports suggest A2A adenosine receptor (A2AAR) could be an attractive choice to counteract adipocyte inflammation and insulin resistance. Thus, an effective A2AAR agonist devoid of any toxicity is highly appealing. Here, we report that indirubin-3′-monoxime (I3M), a derivative of the bisindole alkaloid indirubin, efficiently binds and activates A2AAR which leads to the attenuation of lipid-induced adipocyte inflammation and insulin resistance. Using a combination of in silico virtual screening of potential anti-diabetic candidates and in vitro study on insulin-resistant model of 3T3-L1 adipocytes, we determined I3M through A2AAR activation markedly prevents lipid-induced impairment of the insulin signaling pathway in adipocytes without any toxic effects. While I3M restrains lipid-induced adipocyte inflammation by inhibiting NF-κB dependent pro-inflammatory cytokines expression, it also augments cAMP-mediated CREB activation and anti-inflammatory state in adipocytes. However, these attributes were compromised when cells were pretreated with the A2AAR antagonist, SCH 58261 or siRNA mediated knockdown of A2AAR. I3M, therefore, could be a valuable option to intervene adipocyte inflammation and thus showing promise for the management of insulin resistance and type 2 diabetes.

scholarly journals Modelling for Clinical and Psysiological Evaluation of Diabetes and Glucose Homeostasis

2022 ◽  
pp. 26-34
Author(s):  
Elirea Bornmann
Keyword(s):  
Type 2 Diabetes ◽  
Glucose Homeostasis ◽  
Quantitative Description ◽  
In Vitro Study ◽  
Entire Body ◽  
Social Effect ◽  
History Of

There is a long history of arithmetic simulations in the domain of gluconeogenesis. There are various reasons why frameworks are used. Paradigms have been employed to calculate physiologically relevant parameters from intermediate experimental evidence, to offer a clear quantitative description of pathophysiology processes, and to identify clinical relevance indicators from basic empirical procedures. The creation and application of frameworks in this field has expanded in response to the rising social effect of type 2 diabetes that entails a disruption of the glycemic homeostasis system. The frameworks' emphasis has ranged from depictions of entire body functions to lymphocytes (form “in Vivo” to “in Vitro”) study, following the methodologies of physiologic and medicinal exploration. Framework-based techniques to connecting in vivo and in vitro research, and also multi-resolution systems that combine the two domains, have been presented. The arithmetic and psychological domains have had varying levels of effectiveness and influence.

scholarly journals Anti-inflammatory effects of low-level laser therapy on human periodontal ligament cells: in vitro study

2017 ◽  
Vol 33 (3) ◽  
pp. 469-477 ◽  
Author(s):  
Ji-Hua Lee ◽  
Min-Hsuan Chiang ◽  
Ping-Ho Chen ◽  
Mei-Ling Ho ◽  
Huey-Er Lee ◽  
...  
Keyword(s):  
Laser Therapy ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Vitro Study ◽  
Low Level Laser Therapy ◽  
Periodontal Ligament Cells ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

scholarly journals Vitamin D, adipose tissue, and obesity

2013 ◽  
Vol 15 (3) ◽  
Author(s):  
Julie Marcotorchino ◽  
Franck Tourniaire ◽  
Jean-François Landrier
Keyword(s):  
Type 2 Diabetes ◽  
Vitamin D ◽  
Adipose Tissue ◽  
Cardiovascular Diseases ◽  
Vitamin D Deficiency ◽  
Epidemiological Studies ◽  
The Relationship ◽  
Diabetes And Obesity

AbstractEpidemiological studies have shown a link between vitamin D deficiency and numerous pathologies such as cancers, immunity diseases, cardiovascular diseases, hypertension, type 2 diabetes, and obesity. Recent studies in vitro and in animal models demonstrated an impact of vitamin D on adipose tissue and adipocyte biology. Such observations are of particular interest and provide mechanistic explanations on the relationship between vitamin D deficiency and obesity.

scholarly journals In vitro studies on periodontal ligament cells and enamel matrix derivative

1997 ◽  
Vol 24 (9) ◽  
pp. 685-692 ◽  
Author(s):  
Stina Gestrelius ◽  
Christer Andersson ◽  
Dagny Lidstrom ◽  
Lars Hammarstrom ◽  
Martha Somerman
Keyword(s):  
Periodontal Ligament ◽  
In Vitro Studies ◽  
Enamel Matrix Derivative ◽  
Periodontal Ligament Cells ◽  
Enamel Matrix ◽  
Matrix Derivative
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