scholarly journals Cartilage Repair and Subchondral Bone Migration Using 3D Printing Osteochondral Composites: A One-Year-Period Study in Rabbit Trochlea

2014 ◽  
Vol 2014 ◽  
pp. 1-16 ◽  
Author(s):  
Weijie Zhang ◽  
Qin Lian ◽  
Dichen Li ◽  
Kunzheng Wang ◽  
Dingjun Hao ◽  
...  
Keyword(s):  
3D Printing ◽  
Cartilage Repair ◽  
Subchondral Bone ◽  
Critical Size ◽  
Cartilage Damage ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Defect Center ◽  
Osteochondral Repair ◽  
And Migration

Increasing evidences show that subchondral bone may play a significant role in the repair or progression of cartilage damagein situ. However, the exact change of subchondral bone during osteochondral repair is still poorly understood. In this paper, biphasic osteochondral composite scaffolds were fabricated by 3D printing technology using PEG hydrogel andβ-TCP ceramic and then implanted in rabbit trochlea within a critical size defect model. Animals were euthanized at 1, 2, 4, 8, 16, 24, and 52 weeks after implantation. Histological results showed that hyaline-like cartilage formed along with white smooth surface and invisible margin at 24 weeks postoperatively, typical tidemark formation at 52 weeks. The repaired subchondral bone formed from 16 to 52 weeks in a “flow like” manner from surrounding bone to the defect center gradually. Statistical analysis illustrated that both subchondral bone volume and migration area percentage were highly correlated with the gross appearance Wayne score of repaired cartilage. Therefore, subchondral bone migration is related to cartilage repair for critical size osteochondral defects. Furthermore, the subchondral bone remodeling proceeds in a “flow like” manner and repaired cartilage with tidemark implies that the biphasic PEG/β-TCP composites fabricated by 3D printing provides a feasible strategy for osteochondral tissue engineering application.

scholarly journals 3D-Printed Biopolymers for Tissue Engineering Application

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Xiaoming Li ◽  
Rongrong Cui ◽  
Lianwen Sun ◽  
Katerina E. Aifantis ◽  
Yubo Fan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Three Dimensional ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Printing Technology ◽  
3D Printing Technology ◽  
Potential Applications ◽  
3D Printed ◽  
Dimensional Object

3D printing technology has recently gained substantial interest for potential applications in tissue engineering due to the ability of making a three-dimensional object of virtually any shape from a digital model. 3D-printed biopolymers, which combine the 3D printing technology and biopolymers, have shown great potential in tissue engineering applications and are receiving significant attention, which has resulted in the development of numerous research programs regarding the material systems which are available for 3D printing. This review focuses on recent advances in the development of biopolymer materials, including natural biopolymer-based materials and synthetic biopolymer-based materials prepared using 3D printing technology, and some future challenges and applications of this technology are discussed.

scholarly journals Hydrogel-Hydroxyapatite-Monomeric Collagen Type-I Scaffold with Low-Frequency Electromagnetic field Treatment Enhances Osteochondral Repair in Rabbits

2021 ◽  
Author(s):  
Jiyuan Yan ◽  
Chaoxu Liu ◽  
Yingchi Zhang ◽  
Chang Tu ◽  
Ruizhuo Zhang ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Cartilage Repair ◽  
Collagen Type ◽  
Composite Scaffold ◽  
Cartilage Damage ◽  
Low Frequency ◽  
Collagen Type I ◽  
Type I ◽  
Osteochondral Defects ◽  
Osteochondral Repair

Abstract Background: Cartilage damage is a common medical issue in clinical practice. Complete cartilage repair remains a significant challenge owing to the inferior quality of regenerative tissue. Methods: In this study, a composite scaffold made of Hydroxyapatite-Collagen type-I (HAC) and PLGA-PEG-PLGA thermogel was produced to match the cartilage and subchondral layers in osteochondral defects, respectively. Bone marrow mesenchymal stem cells (BMSC) encapsulated in the thermogel were stimulated by an electromagnetic field (EMF). Results: The scaffold was evaluated in rabbits, and biological characteristic of BMSCs were measured. Results showed that the scaffold with EMF enhances the repair of osteochondral defects in rabbits, and, in particular, cartilage repair. EMF could promote proliferation and chondrogenic differentiation of BMSCs partly by activating the PI3K/AKT/mTOR and Wnt1/LRP6/β-catenin pathway. Conclusion: Hydrogel-Hydroxyapatite-Monomeric Collagen type-I scaffold with low-frequency EMF treatment has the potential to enhance osteochondral repair.

scholarly journals Protein-Based 3D Biofabrication of Biomaterials

2021 ◽  
Vol 8 (4) ◽  
pp. 48
Author(s):  
Mahta Mirzaei ◽  
Oseweuba Valentine Okoro ◽  
Lei Nie ◽  
Denise Freitas Siqueira Petri ◽  
Amin Shavandi
Keyword(s):  
3D Printing ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Native Structure ◽  
Cross Link ◽  
Primary Mechanism ◽  
Protein Gelation ◽  
Physical Gelation ◽  
Printing Processes ◽  
Gel Network

Protein/peptide-based hydrogel biomaterial inks with the ability to incorporate various cells and mimic the extracellular matrix’s function are promising candidates for 3D printing and biomaterials engineering. This is because proteins contain multiple functional groups as reactive sites for enzymatic, chemical modification or physical gelation or cross-linking, which is essential for the filament formation and printing processes in general. The primary mechanism in the protein gelation process is the unfolding of its native structure and its aggregation into a gel network. This network is then stabilized through both noncovalent and covalent cross-link. Diverse proteins and polypeptides can be obtained from humans, animals, or plants or can be synthetically engineered. In this review, we describe the major proteins that have been used for 3D printing, highlight their physicochemical properties in relation to 3D printing and their various tissue engineering application are discussed.

Preparation and Characterization of PLA Film/3D Printing Composite Scaffold for Tissue Engineering Application

2020 ◽  
Vol 21 (4) ◽  
pp. 709-716
Author(s):  
Chenjie Meng ◽  
Jiaming Zhao ◽  
Yuxiang Yin ◽  
Jun Luo ◽  
Lianying Zhao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Composite Scaffold ◽  
Engineering Application ◽  
Tissue Engineering Application

scholarly journals Synthesis of a photocurable acrylated poly(ethylene glycol)-co-poly(xylitol sebacate) copolymers hydrogel 3D printing ink for tissue engineering

RSC Advances ◽  
2019 ◽  
Vol 9 (32) ◽  
pp. 18394-18405 ◽  
Author(s):  
Yicai Wang ◽  
Yuan Li ◽  
Xiaoling Yu ◽  
Qizhi Long ◽  
Tian Zhang
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Ethylene Glycol ◽  
Engineering Application ◽  
Cell Encapsulation ◽  
Tissue Engineering Application ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Printing Ink

A novel acrylated poly(ethylene glycol)-co-poly(xylitol sebacate) (PEXS-A) hydrogel for 3D printing ink and cell encapsulation for tissue engineering application.

scholarly journals Bone Marrow Aspirate Concentrate-Enhanced Marrow Stimulation of Chondral Defects

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Henning Madry ◽  
Liang Gao ◽  
Hermann Eichler ◽  
Patrick Orth ◽  
Magali Cucchiarini
Keyword(s):  
Bone Marrow ◽  
Cartilage Repair ◽  
Subchondral Bone ◽  
Bone Marrow Aspirate ◽  
Critical Role ◽  
Three Dimensional ◽  
Marrow Stimulation ◽  
Chondral Defects ◽  
Osteochondral Repair ◽  
Additional Cell

Mesenchymal stem cells (MSCs) from bone marrow play a critical role in osteochondral repair. A bone marrow clot forms within the cartilage defect either as a result of marrow stimulation or during the course of the spontaneous repair of osteochondral defects. Mobilized pluripotent MSCs from the subchondral bone migrate into the defect filled with the clot, differentiate into chondrocytes and osteoblasts, and form a repair tissue over time. The additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation is thought to enhance cartilage repair as it may provide both an additional cell population capable of chondrogenesis and a source of growth factors stimulating cartilage repair. Moreover, the BMA clot provides a three-dimensional environment, possibly further supporting chondrogenesis and protecting the subchondral bone from structural alterations. The purpose of this review is to bridge the gap in our understanding between the basic science knowledge on MSCs and BMA and the clinical and technical aspects of marrow stimulation-based cartilage repair by examining available data on the role and mechanisms of MSCs and BMA in osteochondral repair. Implications of findings from both translational and clinical studies using BMA concentrate-enhanced marrow stimulation are discussed.

scholarly journals Genetic Deletion of Interleukin-15 Is Not Associated with Major Structural Changes Following Experimental Post-Traumatic Knee Osteoarthritis in Rats

2021 ◽  
Vol 11 (15) ◽  
pp. 7118
Author(s):  
Ermina Hadzic ◽  
Garth Blackler ◽  
Holly Dupuis ◽  
Stephen James Renaud ◽  
Christopher Thomas Appleton ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Subchondral Bone ◽  
Structural Changes ◽  
Cartilage Damage ◽  
Wild Type ◽  
Significant Difference ◽  
Post Traumatic ◽  
Interleukin 15 ◽  
Joint Trauma ◽  
Surgically Induced

Post-traumatic osteoarthritis (PTOA) is a degenerative joint disease, leading to articular cartilage breakdown, osteophyte formation, and synovitis, caused by an initial joint trauma. Pro-inflammatory cytokines increase catabolic activity and may perpetuate inflammation following joint trauma. Interleukin-15 (IL-15), a pro-inflammatory cytokine, is increased in OA patients, although its roles in PTOA pathophysiology are not well characterized. Here, we utilized Il15 deficient rats to examine the role of IL-15 in PTOA pathogenesis in an injury-induced model. OA was surgically induced in Il15 deficient Holtzman Sprague-Dawley rats and control wild-type rats to compare PTOA progression. Semi-quantitative scoring of the articular cartilage, subchondral bone, osteophyte size, and synovium was performed by two blinded observers. There was no significant difference between Il15 deficient rats and wild-type rats following PTOA-induction across articular cartilage damage, subchondral bone damage, and osteophyte scoring. Similarly, synovitis scoring across six parameters found no significant difference between genetic variants. Overall, IL-15 does not appear to play a key role in the development of structural changes in this surgically-induced rat model of PTOA.

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