scholarly journals Pesticidal and Mosquitocidal Activities of Clausena excavata Burm. F. (Rutaceae) against Spodoptera litura (Fab.) (Noctuidae: Lepidoptera) and Aedes aegypti L., Anopheles stephensi Liston, and Culex quinquefasciatus Say. (Diptera: Culicidae)

2014 ◽  
Vol 2014 ◽  
pp. 1-7
Author(s):  
Mathivanan Thangarasu ◽  
Krishnappa Kaliyamoorthy ◽  
Elumalai Kuppusamy
Keyword(s):  
Medicinal Plant ◽  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Spodoptera Litura ◽  
Anopheles Stephensi ◽  
Probit Analysis ◽  
Agricultural Field ◽  
Indian Medicinal Plant ◽  
Potential Source ◽  
Lepidoptera Noctuidae

The aim of this paper is to evaluate the larvicidal and ovicidal activities of hexane, diethyl ether, dichloromethane, ethyl acetate, and methanol extract of Indian medicinal plant, Clausena excavata, at different concentrations against lepidopteran agricultural field pest, Spodoptera litura (Lepidoptera: Noctuidae), and Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus (Diptera: Culicidae). Twenty-five early fourth instar larvae of S. litura and A. aegypti, An. stephensi and C. quinquefasciatus were exposed to various concentrations and were assayed in the laboratory by using specific protocols. The 24 h LC50 and LC90 values of the C. excavata leaf extract was determined by probit analysis. The ovicidal activity were determined against S. litura and A. aegypti, An. stephensi, and C. quinquefasciatus and was assessed 120 hrs after treatment. Results of this study show that the selected Indian medicinal plant C. excavata may be a potential source of natural larvicidal and ovicidal activities against selected pest and vector mosquitoes.

scholarly journals Behavioural response of mosquito vectors Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus to synthetic pyrethroid and organophosphorus-based slow-release insecticidal paint

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Sunil Dhiman ◽  
Kavita Yadav ◽  
B. N. Acharya ◽  
Raj Kumar Ahirwar ◽  
D. Sukumaran
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Anopheles Stephensi ◽  
Slow Release ◽  
Disease Risk ◽  
Mosquito Species ◽  
Escape Time ◽  
Behavioural Avoidance ◽  
Vector Mosquito ◽  
Contact Exposure

Abstract Background The direct toxicological impact of insecticides on vector mosquitoes has been well emphasized; however, behavioural responses such as excito-repellency and physical avoidance as a result of insecticide exposure have not been much studied. We have demonstrated the excito-repellency and behavioural avoidance in certain vector mosquito species on exposure to a slow-release insecticidal paint (SRIP) formulation in addition to direct toxicity. Methods A SRIP formulation developed by the Defence Research and Development Establishment, Gwalior, contains chlorpyriphos, deltamethrin and pyriproxyfen as active insecticides. Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti mosquitoes were used to study the excito-repellency response of the formulation. The experiments were performed in a specially designed dual-choice exposure and escape chamber made of transparent polymethyl methacrylate. For the experiments, the SRIP formulation was applied undiluted at a rate of 8 m2 per kg on 15 cm2 metallic surfaces. Mosquitoes were introduced into the exposure chamber, and observations of the movement of mosquitoes into the escape chamber through the exit portal were taken at 1-min intervals for up to 30 min. Results The evaluated formulation displayed strong excito-repellency against all three tested vector mosquito species. Results showed that the ET50 (escape time 50%) for Ae. aegypti, An. stephensi and Cx. quinquefasciatus was 20.9 min, 14.5 min and 17.9 min for contact exposure (CE) respectively. Altogether in CE, the escape rates were stronger in An. stephensi mosquitoes at different time intervals compared to Ae. aegypti and Cx. quinquefasciatus mosquitoes. The probit analysis revealed that the determined ET did not deviate from linearity for both non-contact exposure (NCE) and placebo exposure (PE) (χ2 ≤ 7.9; p = 1.0) for Ae. aegypti mosquitoes and for NCE (χ2 = 8.3; p = 1.0) and PE (χ2 = 1.7; p = 1.0) treatments in Cx. quinquefasciatus. Mortality (24 h) was found to be statistically higher (F = 6.4; p = 0.02) in An. stephensi for CE but did not vary for NCE (p ≥ 0.3) and PE (p = 0.6) treatments among the tested mosquito species. Survival probability response suggested that all the three tested species displayed similar survival responses for similar exposures (χ2 ≤ 2.3; p ≥ 0.1). Conclusion The study demonstrates the toxicity and strong behavioural avoidance in known vector mosquito species on exposure to an insecticide-based paint formulation. The combination of insecticides in the present formulation will broaden the overall impact spectrum for protecting users from mosquito bites. The efficacy data generated in the study provide crucial information on the effectiveness of the tested formulation and could be useful in reducing the transmission intensity and disease risk in endemic countries.

Larvicidal, pupicidal, repellent and adulticidal activity of Citrus sinensis orange peel extract against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)

2012 ◽  
Vol 111 (4) ◽  
pp. 1757-1769 ◽  
Author(s):  
Kadarkarai Murugan ◽  
Palanisamy Mahesh Kumar ◽  
Kalimuthu Kovendan ◽  
Duraisamy Amerasan ◽  
Jayapal Subrmaniam ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Citrus Sinensis ◽  
Anopheles Stephensi ◽  
Orange Peel ◽  
Adulticidal Activity ◽  
Orange Peel Extract ◽  
Peel Extract

scholarly journals Bacillus thuringiensis isolates entomopathogenic for Culex quinquefasciatus (Diptera: Culicidae) and Anticarsia gemmatalis (Lepidoptera: Noctuidae)

2010 ◽  
Vol 70 (4) ◽  
pp. 1039-1046 ◽  
Author(s):  
V. Gobatto ◽  
SG. Giani ◽  
M. Camassola ◽  
AJP. Dillon ◽  
A. Specht ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Culex Quinquefasciatus ◽  
Reference Strain ◽  
Probit Analysis ◽  
Anticarsia Gemmatalis ◽  
Sds Page ◽  
Pathogenicity Tests ◽  
Lepidoptera Noctuidae ◽  
Reference Strains ◽  
Cry1 Gene

Samples of the Bacillus thuringiensis (Bt) were collected from soil and insects. Eight isolates were selected from rural soil, 15 from urban soil and 11 from insects. These were evaluated for entomopathogenicity against larvae of Anticarsia gemmatalis and Culex quinquefasciatus. The pathogenicity tests showed that a higher percentage of isolates were active against A. gemmatalis (60%) compared to C. quinquefasciatus (31%). Probit analysis (LC50) indicated that against A. gemmatalis four of the isolates presented values similar to the reference strain against A. gemmatalis, while against C. quinquefasciatus one isolate showed an LC50 similar to the reference strain (IPS-82). SDS-PAGE characterisation of two isolates showed a 27 kDa protein fraction related to the Bt subspecies israelensis cytolytic toxin (cyt) gene. One 130 kDa protein, possibly related to the Bt crystal inclusions (cry1) gene, was identified in the other two isolates, which were more toxic for lepidoptera; another isolate presented a protein of 100 kDa. Some new local Bt isolates had similar LC50 probit values to the reference strains.

scholarly journals Estirpes de Bacillus thuringiensis efetivas contra insetos das ordens Lepidoptera, Coleoptera e Diptera

2004 ◽  
Vol 39 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Lílian Botelho Praça ◽  
Andréa Cardoso Batista ◽  
Érica Soares Martins ◽  
Claúdia Brod Siqueira ◽  
Daniel Gerheim de Souza Dias ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Spodoptera Frugiperda ◽  
Anticarsia Gemmatalis ◽  
Anthonomus Grandis ◽  
Lepidoptera Noctuidae

O objetivo deste trabalho foi selecionar entre 300 estirpes de Bacillus thuringiensis as efetivas simultaneamente contra larvas de Spodoptera frugiperda J.E. Smith e Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae), Anthonomus grandis Boheman (Coleoptera: Curculionidae), Aedes aegypti Linnaeus e Culex quinquefasciatus Say (Diptera: Culicidae). Foram selecionadas duas estirpes de B. thuringiensis, denominadas S234 e S997, que apresentaram atividade contra as três ordens de insetos. As estirpes foram caracterizadas por métodos morfológicos, bioquímicos e moleculares. As mesmas apresentaram duas proteínas principais de 130 e 65 kDa, produtos de reação em cadeia da polimerase de tamanho esperado para a detecção dos genes cry1Aa, cry1Ab, cry1Ac, cry1B e cry2 e cristais bipiramidais, cubóides e esféricos.

scholarly journals Beauveria bassiana (Clavicipitaceae): a potent fungal agent for controlling mosquito vectors of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae)

RSC Advances ◽  
2017 ◽  
Vol 7 (7) ◽  
pp. 3838-3851 ◽  
Author(s):  
Chinnasamy Ragavendran ◽  
Nawal Kishore Dubey ◽  
Devarajan Natarajan
Keyword(s):  
Aedes Aegypti ◽  
Beauveria Bassiana ◽  
Bioactive Compounds ◽  
Culex Quinquefasciatus ◽  
Anopheles Stephensi ◽  
Mosquito Vectors ◽  
Laboratory Conditions

The efficacy of bioactive compounds identified from Beauveria bassiana extracts as effective larvicidal and pupicidal agents against Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti mosquito vectors under laboratory conditions are studied.

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