scholarly journals Mosquito Larvicidal Constituents from the Ethanol Extract ofInula racemosaHook. f. Roots againstAedes albopictus

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Qing He ◽  
Xin Chao Liu ◽  
Rui Qi Sun ◽  
Zhi Wei Deng ◽  
Shu Shan Du ◽  
...  
Keyword(s):  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
Ethanol Extract ◽  
Natural Compounds ◽  
Fourth Instar ◽  
Active Constituents ◽  
Inula Racemosa ◽  
Asian Tiger

The aim of this research was to determine larvicidal activity of the ethanol extract ofInula racemosaHook. f. (Compositae) roots against the larvae of the Culicidae mosquitoAedes albopictusand to isolate any larvicidal constituents from the extract. Based on bioactivity-guided fractionation, 11,13-dihydroisoalantolactone (1), macrophyllilactone E (2), 5α-epoxyalantolactone (3), and epoxyisoalantolactone (4) were isolated and identified as the active constituents. Compounds1and2exhibited strong larvicidal activity against the early fourth-instar larvae ofA. albopictuswith LC50values of 21.86 μg/mL and 18.65 μg/mL, respectively, while the ethanol extract had a LC50value of 25.23 μg/mL. Compounds3and4also possessed larvicidal activity against the Asian tiger mosquitoes with LC50values of 29.37 μg/mL and 35.13 μg/mL, respectively. The results indicated that the ethanol extract ofI. racemosaand the four isolated constituents have potential for use in the control ofA. albopictuslarvae and could be useful in the search of newer, safer, and more effective natural compounds as larvicides.

Larvicidal activity of the essential oil of Youngia japonica aerial parts and its constituents against Aedes albopictus

2015 ◽  
Vol 70 (1-2) ◽  
pp. 1-6 ◽  
Author(s):  
Xin Chao Liu ◽  
Qiyong Liu ◽  
Xu Bo Chen ◽  
Qi Zhi Liu ◽  
Zhi Long Liu
Keyword(s):  
Mass Spectrometry ◽  
Essential Oil ◽  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
Fourth Instar ◽  
Gas Chromatography Mass Spectrometry ◽  
Active Compounds ◽  
Aerial Parts ◽  
Potential Sources ◽  
Oil Gas

Abstract The aim of this study was to evaluate the larvicidal activity of the essential oil of Youngia japonica aerial parts against the larvae of Aedes albopictus and to isolate any active compounds from the oil. Gas chromatography-mass spectrometry (GC-MS) analyses revealed the presence of 31 compounds, with menthol (23.53%), α-asarone (21.54%), 1,8-cineole (5.36%), and caryophyllene (4.45%) as the major constituents. Bioactivity-directed chromatographic separation of the oil led to the isolation of menthol and α-asarone as active compounds. The essential oil of Y. japonica exhibited larvicidal activity against the fourth instar larvae of A. albopictus with an LC50 value of 32.45 μg/mL. α-Asarone and menthol possessed larvicidal activity against the fourth instar larvae of A. albopictus with LC50 values of 24.56 μg/mL and 77.97 μg/mL, respectively. The results indicate that the essential oil of Y. japonica aerial parts and the two constituents can be potential sources of natural larvicides.

scholarly journals Development of cellulose nanocrystal-stabilized Pickering emulsions of massoia and nutmeg essential oils for the control of Aedes albopictus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Seon-Mi Seo ◽  
Jae-Woo Lee ◽  
Jonghyun Shin ◽  
Jun-Hyung Tak ◽  
Jinho Hyun ◽  
...  
Keyword(s):  
Essential Oils ◽  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
Cellulose Nanocrystal ◽  
Asian Tiger Mosquito ◽  
Pickering Emulsions ◽  
Benzyl Benzoate ◽  
Plant Essential Oils ◽  
Asian Tiger ◽  
Larvicidal Activities

AbstractWe investigated the larvicidal potential of 10 plant essential oils (EOs) against the Asian tiger mosquito Aedes albopictus. Among the EOs, larvicidal activity against Ae. albopictus was strongest in those derived from massoia (Massoia aromatica) and nutmeg (Myristica fragrans). Larvicidal activities of massoia and nutmeg EOs against Ae. albopictus were 95.0% and 85.0% at 50 μg/mL, respectively. A total of 4 and 14 compounds were identified from massoia and nutmeg, respectively, and two massoia lactones, C10 and C12, were isolated from massoia EO. Among the identified compounds, benzyl salicylate, terpinolene, C12 massoia lactone, sabinene, benzyl benzoate, methyl eugenol, and C10 massoia lactone exhibited the strong larvicidal activity. Cellulose nanocrystal (CNC)-stabilized Pickering emulsions of massoia and nutmeg EOs were developed to overcome the insolubility of EOs in water. CNC/massoia and CNC/nutmeg emulsions were stable for at least 10 days, and larvicidal activities of CNC/massoia PE and CNC/nutmeg were higher than those of crude massoia and nutmeg EOs. This study presents a CNC-stabilized PE, a suitable formulation for EOs, as a potential larvicide against Ae. albopictus.

Larvicidal Activity of Steroidal Saponins from Dracaena arborea on Aedes albopictus

2016 ◽  
Vol 17 (12) ◽  
pp. 1036-1042
Author(s):  
Prosper C.B. Nya ◽  
Riccardo Moretti ◽  
Marcello Nicoletti ◽  
Maurizio Calvitti ◽  
Lamberto Tomassini
Keyword(s):  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
Steroidal Saponins

scholarly journals A Linkage-Based Genome Assembly for the Mosquito Aedes albopictus and Identification of Chromosomal Regions Affecting Diapause

Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 167
Author(s):  
John H. Boyle ◽  
Pasi M. A. Rastas ◽  
Xin Huang ◽  
Austin G. Garner ◽  
Indra Vythilingam ◽  
...  
Keyword(s):  
Linkage Map ◽  
Aedes Albopictus ◽  
Dna Sequences ◽  
Genome Assembly ◽  
Single Copy ◽  
Health Concern ◽  
Candidate Snps ◽  
Single Location ◽  
Asian Tiger ◽  
Photoperiodic Diapause

The Asian tiger mosquito, Aedes albopictus, is an invasive vector mosquito of substantial public health concern. The large genome size (~1.19–1.28 Gb by cytofluorometric estimates), comprised of ~68% repetitive DNA sequences, has made it difficult to produce a high-quality genome assembly for this species. We constructed a high-density linkage map for Ae. albopictus based on 111,328 informative SNPs obtained by RNAseq. We then performed a linkage-map anchored reassembly of AalbF2, the genome assembly produced by Palatini et al. (2020). Our reassembled genome sequence, AalbF3, represents several improvements relative to AalbF2. First, the size of the AalbF3 assembly is 1.45 Gb, almost half the size of AalbF2. Furthermore, relative to AalbF2, AalbF3 contains a higher proportion of complete and single-copy BUSCO genes (84.3%) and a higher proportion of aligned RNAseq reads that map concordantly to a single location of the genome (46%). We demonstrate the utility of AalbF3 by using it as a reference for a bulk-segregant-based comparative genomics analysis that identifies chromosomal regions with clusters of candidate SNPs putatively associated with photoperiodic diapause, a crucial ecological adaptation underpinning the rapid range expansion and climatic adaptation of A. albopictus.

scholarly journals Bioassay-guided isolation and identification of Aedes aegypti larvicidal and biting deterrent compounds from Veratrum lobelianum

2018 ◽  
Vol 16 (1) ◽  
pp. 324-332 ◽  
Author(s):  
Nurhayat Tabanca ◽  
Zulfiqar Ali ◽  
Ulrich R. Bernier ◽  
Nancy Epsky ◽  
Ayse Nalbantsoy ◽  
...  
Keyword(s):  
Larvicidal Activity ◽  
Ethanol Extract ◽  
Ethyl Linoleate ◽  
Significant Activity ◽  
Isolation And Identification ◽  
Human Carcinoma ◽  
Ethyl Palmitate ◽  
H Nmr ◽  
Bioassay Guided Fractionation ◽  
Nmr Techniques

AbstractAn ethanol extract fromVeratrum lobelianumBernh. rhizomes showed larvicidal activity with LC50values of 11.79 ppm and 89.9 ppm against 1stand 4thinstar larvae, respectively, at 24 h post-treatment. The extract also showed proportion not biting (PNB) value of 0.76 at 100 μg/cm2against females ofAe.aegypti. Systematic bioassay-guided fractionation ofV.lobelianumextract resulted in the isolation of five compounds that were identified as ethyl palmitate (1), ethyl linoleate (2), β-sitosterol (3), resveratrol (4) and oxyresveratrol (5) by GC-MS,1H-NMR, and13C-NMR techniques, comparison with literature data, and confirmation with authentic compounds. Compound2exhibited larvicidal activity with an LC50value of 24.1 (22.0-26.2) ppm whereas1was inactive. β-Sitosterol (3) displayed the highest larvicidal activity with LC50= 1.7 (1.3-12.3) ppm and LC90= 5.1 (3.4-13.8) ppm. Compounds4and5had larvicidal activity with LC50values of 18.5 (15.3-23.3) and 22.6 (19.0-26.8) ppm, respectively, and had an equivalent PNB values of 0.75 at 25 nmol/cm2. In addition,4and5were explored for their human-based repellency againstAe.aegypti, attractiveness against male medfliesCeratitis capitata, and also evaluated against series of human carcinoma cells (A549, HEK293, HeLa, SH-SY5Y); however, no significant activity was found.

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