scholarly journals Cytotoxic Activities against Breast Cancer Cells of LocalJusticia gendarussaCrude Extracts

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Zahidah Ayob ◽  
Siti Pauliena Mohd Bohari ◽  
Azman Abd Samad ◽  
Shajarahtunnur Jamil
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Leaf Extract ◽  
Cancer Cell Lines ◽  
Peninsular Malaysia ◽  
Breast Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Leaf Extracts ◽  
High Cytotoxicity

Justicia gendarussamethanolic leaf extracts from five different locations in the Southern region of Peninsular Malaysia and two flavonoids, kaempferol and naringenin, were tested for cytotoxic activity. Kaempferol and naringenin were two flavonoids detected in leaf extracts using gas chromatography-flame ionization detection (GC-FID). The results indicated that highest concentrations of kaempferol and naringenin were detected in leaves extracted from Mersing with 1591.80 mg/kg and 444.35 mg/kg, respectively. Positive correlations were observed between kaempferol and naringenin concentrations in all leaf extracts analysed with the Pearson method. The effects of kaempferol and naringenin from leaf extracts were examined on breast cancer cell lines (MDA-MB-231 and MDA-MB-468) using MTT assay. Leaf extract from Mersing showed high cytotoxicity against MDA-MB-468 and MDA-MB-231 with IC50values of 23 μg/mL and 40 μg/mL, respectively, compared to other leaf extracts. Kaempferol possessed high cytotoxicity against MDA-MB-468 and MDA-MB-231 with IC50values of 23 μg/mL and 34 μg/mL, respectively. These findings suggest that the presence of kaempferol in Mersing leaf extract contributed to high cytotoxicity of both MDA-MB-231 and MDA-MB-468 cancer cell lines.

Cytotoxic activity and anti-cancer potential of Ontario grown onion extracts against breast cancer cell lines

2018 ◽  
Vol 8 (3) ◽  
pp. 159 ◽  
Author(s):  
Meghan Fragis ◽  
Abdulmonem I. Murayyan ◽  
Suresh Neethirajan
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Cancer Line ◽  
Mcf 7

Background: Breast cancer is the most commonly diagnosed cancer and the second leading cause of cancer deaths among Canadian women. Cancer management through changes in lifestyle, such as increased intake of foods rich in dietary flavonoids, have been shown to decrease the risk associated with breast, liver, colorectal, and upper-digestive cancers in epidemiologic studies. Onions are high in flavonoid content and one of the most common vegetables. Additionally, onions are used in most Canadian cuisines.Methods: We investigated the effect of five prominent Ontario grown onion (Stanley, Ruby Ring, LaSalle, Fortress, and Safrane) extracts on two subtypes of breast cancer cell lines: a triple negative breast cancer line MDA-MB-231 and an ER+ breast cancer line MCF-7.Results: These onion extracts elicited strong anti-proliferative, anti-migratory, and cytotoxic activities on both the cancer cell lines. Flavonoids present in these onion extracts induced apoptosis, cell cycle arrest in the G2/M phase, and a reduction in mitochondrial membrane potential at dose-dependent concentrations. Onion extracts were more effective against MDA-MB-231 compared to the MCF-7 cell line. Conclusion: In this study, we investigated the extracts synthesized from Ontario-grown onion varieties in inducing anti-migratory, cytostatic, and cytotoxic activities in two sub-types of human breast cancer cell lines. Anti-tumor activity of these extracts depends upon the varietal and can be formulated into nutraceuticals and functional foods for the wellbeing of cancer patients. Overall, the results suggest that onion extracts are a good source of flavonoids with anti-cancerous properties.Keywords: onion extracts; flavonoids; anti-proliferative; breast cancer; cytotoxic activity

Vermentino Leaf Extracts Inhibit Tumorigenicity via FASN Downregulation in Breast Cancer Cell Lines

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Andrea Floris ◽  
Michael Mazarei ◽  
Jennifer Zhou ◽  
Xi Yang ◽  
Maria Lauda Tomasi
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Leaf Extracts

Ethanolic neem (Azadirachta indica A. Juss) leaf extract induces apoptosis and inhibits the IGF signaling pathway in breast cancer cell lines

2012 ◽  
Vol 2 (1) ◽  
pp. 59-68 ◽  
Author(s):  
Perumal Elumalai ◽  
Dharmalingam Nandagopal Gunadharini ◽  
Kalimuthu Senthilkumar ◽  
Sivanantham Banudevi ◽  
Ramachandran Arunkumar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Signaling Pathway ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Igf Signaling

scholarly journals Synthesis, characterization and cytotoxicity appraisal of original 1, 2, 3-Triazole derivatives, against breast cancer cell lines (MDA-MB-231)

2019 ◽  
Vol 9 (4) ◽  
pp. 305-310 ◽  
Author(s):  
Mohammed Khalaf Mohammed ◽  
Zainab Al-Shuhaib ◽  
Ali A. A. Al-Shawi
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Triazole Derivatives ◽  
Ic50 Values

The present study established the efficient separate synthesis of four unique 1, 2, 3-triazole derivatives (M1, M2, M3, M4) via conducting 1,3-dipolar cycloaddition of N-((4-azidophenyl) sulfonyl) acetamide, with substituted N-phenylmaleimide. FTIR, 1H NMR, 13C NMR, and mass spectra were utilized for the characterization of the triazoles. The cytotoxic activities of these compounds, with regards to breast cancer cell lines (MDA-MB-231), were then evaluated. The cytotoxicity pre-screening outcomes for 100 µM portrayed a variety of actions, while the IC50 values with concentrations of 0-500 µM for 48 hours, the results are 2.542, 2.929, 2.429, and 2.864 µM for the compounds M1, M2, M3, and M4 respectively. Remarkably, the M2 and M4 para-substituted compounds exhibited superior IC50 values, in comparison to the M1 and M3 ortho-substituted compounds. This suggests that the M1 and M3 compounds have the potential to perform as against breast cancer.

scholarly journals Evaluating the Synergistic Effects of Carica papaya Linn, Vernonia amygdalina Delile, Artemisinin and Iodine As Potential Agents in the Management of Breast Cancer

2018 ◽  
Vol 4 (Supplement 2) ◽  
pp. 122s-122s
Author(s):  
J. Nowak
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Vernonia Amygdalina

Background: The rise in breast cancer prevalence rate across the globe and in Africa is a public health issue. Accessibility to the effective and affordable therapeutic options for the management of breast cancer in sub-Saharan Africa is challenging due to high costs, questionable effectiveness and toxicity of treatments available. Studies have documented the use of complimentary and alternative medicine in contemporary cancer treatment in form of standardized phytomedicines. However, the scientific evidence on their efficacies, synergy, safety and adequacy is still lacking. Vernonia amygdalina Delile (leaf extract). Furthermore, Carica papaya Linn (leaf extract), artemisinin and molecular iodine have been previously reported to have activity against breast cancer but their synergistic effect has not been validated. Aim: This study seeks to evaluate synergistic effects of Carica papaya Linn (leaf extract), Vernonia amygdalina Delile (leaf extract), artemisinin and molecular iodine against breast cancer cell lines in vitro and safety profile in selected animal models in vivo. Four specific objectives where stated: (i) to determine inhibitory concentration (IC50) of the plant and compound combinations in vitro against breast cancer cell lines; (ii) to determine effective dose (ED50) of most effective combination(s) against cancer cell lines using animal models (in vivo); (iii) to assess the acute, subacute, chronic toxicity profile of most efficacious combination against breast cancer cell lines; (iv) to identify and characterize bioactive compounds in the combination that shows the highest activity against breast cancer cell lines. Methods: Breast cancer cell lines: MCF 7, MDA-MB-231 will be used to determine IC50 of combinations in vitro. MTT assay will be applied for quantification of viable cells in culture. Both positive and negative controls will be adopted in the study. Mice and rats animal models will be used for toxicity study and determination of ED50 of most active combinations. Bioactive compounds in combination(s) will be identified using chromatographic and spectroscopic methods. Standard procedures according to WHO. Graph Prism computer software will be used to obtain LD50. The results will be statistically analyzed using Student t-test and one-way analysis of variance (ANOVA) using SPSS 16 (Statistical Package for Social Sciences) software. Results: Study in progress. Conclusion: This study will provide a significant contribution into aspects of herbal medicine in breast cancer research, as there is limited research and development efforts in respect to new products to treat breast cancer in the African context. The development of natural, efficacious, cost effective, safer, easier accessible breast cancer management alternative is anticipated as a result of the study. Some of the compounds found in the chosen plants may be used as templates for development of new anticancer product.

New macrocyclic diterpenes fromEuphorbia connataBoiss. with cytotoxic activities on human breast cancer cell lines

2014 ◽  
Vol 29 (7) ◽  
pp. 607-614 ◽  
Author(s):  
Somayeh Shadi ◽  
Hojjatollah Saeidi ◽  
Mustafa Ghanadian ◽  
Mohammad Reza Rahimnejad ◽  
Mahmoud Aghaei ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Human Breast Cancer ◽  
Cancer Cell Lines ◽  
Human Breast Cancer Cell ◽  
Breast Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Human Breast

scholarly journals Bursera copallifera Extracts Have Cytotoxic and Migration-Inhibitory Effects in Breast Cancer Cell Lines

2018 ◽  
Vol 17 (3) ◽  
pp. 654-664 ◽  
Author(s):  
Fabiola Domínguez ◽  
Paola Maycotte ◽  
Adilene Acosta-Casique ◽  
Sofía Rodríguez-Rodríguez ◽  
Diego A. Moreno ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Phenolic Compounds ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Leaf Extracts ◽  
Phenolic Components

Plants from the Bursera genus are widely distributed in the tropical dry forests of Mexico. In traditional medicine, extracts from different species of Bursera have been used for a wide range of biological activities, including the treatment of cancer-related symptoms. Compounds present in the Bursera genus include lignans, flavonoids, steroids, short-chain aliphatic alkanes, acetates, alcohols, ketones, and terpenoids. In some instances, secondary metabolites of these classes of compounds may induce cytotoxicity, and therefore we sought to investigate the effects of B. copallifera leaf extracts in breast cancer cell lines to evaluate their potential therapeutic value for the treatment of breast cancer, one of the most prevalent types of cancer in women worldwide. Two B. copallifera leaf extracts exerted cytotoxic effects on both the MCF7 and MDA-MB-231 breast cancer cell line models. The cytotoxic effect was more evident in the MDA-MB-231 triple negative cell line inhibiting also the migration of these cells. We identified hydroxycinnamic acid and flavonol derivatives as major phenolic components of the extracts. Our results strongly suggest a potential use of the Bursera leaf extracts rich in phenolic compounds, their individual phenolic compounds, or their combinations for the treatment of breast cancer.

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