scholarly journals A Validated HPLC-DAD Method for Simultaneous Determination of Etodolac and Pantoprazole in Rat Plasma

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ali S. Abdelhameed ◽  
Samar A. Afifi
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Rat Plasma ◽  
Array Detector ◽  
Therapeutic Drug ◽  
Ich Guidelines ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Interday Precision

A simple, sensitive, and accurate HPLC-DAD method has been developed and validated for the simultaneous determination of pantoprazole and etodolac in rat plasma as a tool for therapeutic drug monitoring. Optimal chromatographic separation of the analytes was achieved on a Waters Symmetry C18 column using a mobile phase that consisted of phosphate buffer pH~4.0 as eluent A and acetonitrile as eluent B in a ratio of A : B, 55 : 45 v/v for 6 min, pumped isocratically at a flow rate of 0.8 mL min−1. The eluted analytes were monitored using photodiode array detector set to quantify samples at 254 nm. The method was linear withr2=0.9999for PTZ andr2=0.9995for ETD at a concentration range of 0.1–15 and 5–50 μgmL−1for PTZ and ETD, respectively. The limits of detection were found to be 0.033 and 0.918 μgmL−1for PTZ and ETD, respectively. The method was statistically validated for linearity, accuracy, precision, and selectivity following the International Conference for Harmonization (ICH) guidelines. The reproducibility of the method was reliable with the intra- and interday precision (% RSD) <7.76% for PTZ and <7.58 % for ETD.

Simultaneous Determination of Co-administrated Deflazacort, Aprepitant and Granisetron in Dosage Forms and Spiked Human Plasma by RP-HPLC/PAD

2019 ◽  
Vol 57 (9) ◽  
pp. 790-798 ◽  
Author(s):  
Mahmoud A Tantawy ◽  
Soheir Alweshahy ◽  
Dalia A Elshabasy ◽  
Nadia F Youssef
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Reversed Phase ◽  
Dosage Forms ◽  
Array Detector ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Photodiode Array Detector ◽  
Photodiode Array

Abstract A selective reversed phase high performance liquid chromatography/photodiode array detector (RP-HPLC/PAD) method has been developed for simultaneous determination of the three co-administrated deflazacort, aprepitant and granisetron drugs used with chemotherapy. The three cited drugs have been chromatographed on C18 column using a mobile phase consisting of acetonitrile–0.2% v/v triethylamine (80:20 v/v, pH of 6.6 ± 0.05) with isocratic elution and monitored by photodiode array at 220 nm. International conference on harmonization (ICH) guidelines were followed to validate the developed method. Successful application of the developed method was assessed by the simultaneous determination of the studied drugs in pure forms, dosage forms and plasma samples in the ranges of 0.2–20, 0.4–40 and 0.2–20 μg/mL for deflazacort, aprepitant and granisetron, respectively.

scholarly journals Validation of a Capillary Electrophoresis Method for the Simultaneous Determination of Amlodipine Besylate and Valsartan in Pharmaceuticals and Human Plasma

2011 ◽  
Vol 94 (2) ◽  
pp. 498-502 ◽  
Author(s):  
Ahmed O Alnajjar
Keyword(s):  
Capillary Electrophoresis ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
Array Detector ◽  
Therapeutic Drug ◽  
Column Temperature ◽  
Photodiode Array Detector ◽  
Electrophoresis Method ◽  
Photodiode Array

Abstract A rapid, simple, and sensitive capillary electrophoresis (CE) method was developed and validated for the simultaneous determination of amlodipine (AML) and valsartan (VAL) in pharmaceuticals and human plasma using a UV photodiode array detector. Electrophoretic conditions were optimized to improve separation, sensitivity, and rapidity. The optimal conditions were 25 mM phosphate buffer at pH 8.0, injection time 10.0 s, voltage 25 kV, and column temperature 25C, with detection at 214 nm. The method was found to be linear in the range of 1.035 and 1.0350 mg/L, with weighted regression 0.9999 and 0.9994, for AML and VAL, respectively. Validation of the method showed acceptable intraday and interday accuracy (85.595.3) and precision (RSD 1.644.2) in pharmaceutical formulation and human plasma analysis. The sensitivity of the method was enhanced by both optimization of the CE procedure and preconcentration performed by liquidliquid extraction. The LOD for both AML and VAL was 0.03 mg/L, which allows analysis at the level of the drugs possibly found in human plasma. Therefore, the proposed method is suitable for QC in pharmaceutical laboratories and therapeutic drug monitoring in clinical laboratories.

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR RILPIVIRINE HYDROCHLORIDE IN BULK AND USE IN ANALYSIS OF PREPARED NANOSUSPENSION

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (04) ◽  
pp. 42-46
Author(s):  
Madhuri Manchala ◽  
◽  
Vijaya Sri Kanagala ◽  
Ganapath Vinay Jain
Keyword(s):  
Homogenization Method ◽  
Hplc Method ◽  
Array Detector ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Development And Validation ◽  
Tablet Dosage

A simple, precise, accurate and robust RP-HPLC-PDA method was developed and validated for the determination of rilpivirine hydrochloride in tablet dosage forms. Reverse-phase chromatography was performed on a BDS hypersil (250 mm × 4.6 mm, 6 μm) column of Waters HPLC with Empower software and with a photodiode array detector. Methanol: acetonitrile: water 80:13.5:6.5 (v/v) was used as the mobile phase at a flow rate of 1 mL min-1 with PDA detection at 306 nm. Rilpivirine hydrochloride nanosuspension was prepared by using an ultrasonic homogenization method. Linearity was observed in the concentration range of 0.1–10 μg mL-1 with regression equation y = 508856X+46908 (R2 = 0.9998). The method was validated as per ICH guidelines. The RSD for intra-day (1.31- 0.67) and inter-day (1.69-1.59) precision was found to be less than 2%. The developed method is simple, precise and robust for the determination of rilpivirine hydrochloride and is successfully applied for the nanosuspension.

Method Development and Validation for the Determination of Linezolid Drug in Human Plasma by Reversed-Phase High-Performance Liquid Chromatography

2021 ◽  
Vol 17 ◽  
Author(s):  
Missoum Amina ◽  
Kahina Hamza ◽  
Fatiha Malki ◽  
Abderrezak Hamdi ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Particle Size ◽  
Flow Rate ◽  
Chromatographic Separation ◽  
Phosphate Buffer ◽  
Mobile Phase ◽  
Array Detector ◽  
Therapeutic Drug ◽  
Pharmacokinetic Studies ◽  
Photodiode Array Detector ◽  
Photodiode Array

Objective: Linezolid is a significant antibiotic used against severe infections initiated by multi-resistant bacterial pathogens. Method: Linezolid extraction from plasma is obtained using methanol. Chromatographic separation is achieved isocratically on a C18 column [Zorbax C18, 5 µm particle size, 150 mm ˟ 4.6 mm] making use of a mobile phase of acetonitrile / 0.05 M phosphate buffer, pH = 4.5 (30 : 70 v/v) at a flow rate of 1.2 mL/min with photodiode array detector DAD, at a wavelength of 256 nm. Method: Linezolid extraction from plasma is obtained using methanol. Chromatographic separation is achieved isocratically on a C18 column [Zorbax C18, 5 µm particle size, 150 mm ˟ 4.6 mm] making use of a mobile phase of acetonitrile / 0.05 M phosphate buffer, pH = 4.5 (30 : 70 v/v) at a flow rate of 1.2 mL/min with photodiode array detector DAD, at a wavelength of 256 nm. Results : The retention time of linezolid was 2.5 min. The analytical method was linear (r2 > 0.998) over the calibration range of 0.30 to 50.0 µg/mL. The extraction recoveries of linezolid range from 71.03 to 91.93 %. The limit of quantification and the limit of detection were 0.112 µg and 0.037 µg, respectively. The RSDs for intraday and interday assays were < 7.77 and 4.32 %, respectively. The intraday and interday accuracies were in the range 80.6-112 % and 77.44-104.85 %, respectively. Conclusion: The applied method is precise, accurate and appropriate for pharmacokinetic studies and therapeutic drug monitoring of linezolid in routine clinical practice.

Quantitative analysis of fourteen synthetic dyes in jelly and gummy candy by ultra performance liquid chromatography

2014 ◽  
Vol 6 (15) ◽  
pp. 5872-5878 ◽  
Author(s):  
Yi Yang ◽  
Jing Zhang ◽  
Bing Shao
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Analytical Method ◽  
Ultra Performance Liquid Chromatography ◽  
Array Detector ◽  
Synthetic Dyes ◽  
Photodiode Array Detector ◽  
Photodiode Array

An analytical method was developed for the simultaneous determination of 14 dyes in jelly and gummy candy using ultra performance liquid chromatography with a photodiode array detector.

scholarly journals DETERMINATION OF LUTEOLIN FROM EXTRACTIONS OF Helicteres hirsuta BY HPLC

2019 ◽  
Vol 128 (1B) ◽  
pp. 43
Author(s):  
Lê Trung Hiếu ◽  
Lê Lâm Sơn ◽  
Nguyễn Minh Nhung ◽  
Hồ Xuân Anh Vũ ◽  
Trần Thị Văn Thi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Array Detector ◽  
Plant Parts ◽  
Aerial Parts ◽  
Photodiode Array Detector ◽  
Photodiode Array

<p>High performance liquid chromatography coupled with photodiode array detector (HPLC- DAD) has been reported to quantify isolated compounds. This work was designed, therefore, to develop an HPLC-DAD system to determine luteolin in extractive solutions from <em>Helicteres hirsuta</em>. Luteolin was analyzed on a RP- C18 column, using a mobile phase including: acetonitrile - 0.1% acid phosphoric (v/v), under the following conditions detecting wavelength: 347 nm; flow rate: 0.5 mL/min, and the volume of injecting sample: 10 μL. The HPLC system was carried out at ambient temperature. The method showed linearity for luteolin in the range to 0.02 from 1 mg/mL, and the recovery of luteolin was 94.07 ± 0.64. Contents of  luteolin in methanol extracts from the plant parts of <em>H. hirsuta</em> (including: branch, leaf, fruit and aerial parts) were determined with value of 49.06 ±0.46, 142.89 ±0.53, 56.61±0.62 and 91.15±0.42 µg/g, respectively.</p>

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