scholarly journals Biosynthesis of Silver Nanoparticles by Marine Invertebrate (Polychaete) and Assessment of Its Efficacy against Human Pathogens

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Reena Singh ◽  
Sunil Kumar Sahu ◽  
Muthusamy Thangaraj
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Marine Invertebrate ◽  
Average Particle Size ◽  
Silver Ions ◽  
Physical Method ◽  
Average Particle ◽  
Human Pathogens ◽  
X Ray ◽  
Vis Spectroscopy

Synthesis of metallic nanoparticles by chemical and physical method makes the process often cumbersome due the usage of toxic and expensive chemicals. The present study reports the biosynthesis of silver nanoparticles using marine invertebrate (polychaete) extract at room temperature. The ultraviolet-visible (UV-Vis) spectroscopy revealed the formation of silver nanoparticles (AgNPs) by exhibiting the typical surface plasmon absorption maximum at 418–420 nm. Structure and composition of AgNPs were analyzed by atomic force microscopy (AFM). Average particle size of AgNPs ranged from 40 to 90 nm, confirmed by scanning electron microscopy (SEM) analysis. The energy-dispersive X-ray spectroscopy (EDX) of the nanoparticles dispersion confirmed the presence of elemental silver signal, whereas X-ray diffraction (XRD) substantiated the crystalline nature of synthesized nanoparticle. Fourier transform infrared spectroscopy (FTIR) spectral analysis showed the presence of amides phenols, ethers, and fatty acids as major biomolecules responsible for the reduction of silver ions. The possible mechanism responsible for the synthesis of AgNPs by these biomolecules was also illustrated by chemical reactions. The synthesized AgNPs showed comparatively good antibacterial activity against the tested human pathogens. This study advocates that not only plants and microbes but also marine invertebrates do have potential for synthesizing nanoparticles by a cost-effective and eco-friendly approach.

scholarly journals Synthesis and Characterization of Silver Nanoparticles Using Cannonball Leaves and Their Cytotoxic Activity against MCF-7 Cell Line

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Preetha Devaraj ◽  
Prachi Kumari ◽  
Chirom Aarti ◽  
Arun Renganathan
Keyword(s):  
Silver Nanoparticles ◽  
Cell Line ◽  
Average Particle Size ◽  
Cancer Cell Line ◽  
Silver Ions ◽  
Average Particle ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
Vis Spectroscopy ◽  
Mcf 7

Cannonball (Couroupita guianensis) is a tree belonging to the family Lecythidaceae. Various parts of the tree have been reported to contain oils, keto steroids, glycosides, couroupitine, indirubin, isatin, and phenolic substances. We report here the synthesis of silver nanoparticles (AgNPs) using cannonball leaves. Green synthesized nanoparticles have been characterized by UV-Vis spectroscopy, SEM, TEM, and FTIR. Cannonball leaf broth as a reducing agent converts silver ions to AgNPs in a rapid and ecofriendly manner. The UV-Vis spectra gave surface plasmon resonance peak at 434 nm. TEM image shows well-dispersed silver nanoparticles with an average particle size of 28.4 nm. FTIR showed the structure and respective bands of the synthesized nanoparticles and the stretch of bonds. Green synthesized silver nanoparticles by cannonball leaf extract show cytotoxicity to human breast cancer cell line (MCF-7). Overall, this environmentally friendly method of biological silver nanoparticles production provides rates of synthesis faster than or comparable to those of chemical methods and can potentially be used in various human contacting areas such as cosmetics, foods, and medical applications.

scholarly journals GREEN SYNTHESIS OF SILVER NANOPARTICLES USING GRAPE POMACE WASTE FOR THE DEGRADATION OF PHARMACEUTICALS DRUGS AND ORGANIC DYES

2019 ◽  
pp. 37-42
Author(s):  
Margarita Skiba ◽  
Viktoria Vorobyova ◽  
Oleksandr Pivovarov
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Metallic Nanoparticles ◽  
Average Particle Size ◽  
Silver Ions ◽  
Grape Pomace ◽  
Chemical Extraction ◽  
Average Particle ◽  
Ag Nps ◽  
Visible Absorption

The green synthesis of metallic nanoparticles paved the way to improve and protect the environment bydecreasing the use of toxic chemicals. A simple and eco-friendly method for silver nanoparticles (AgNPs) synthesis employing the aqueous extract obtained from grape pomace by plasma-chemical extraction technique was developed. The reduction of silver ions in solution was monitored using UV–visible absorption spectroscopy. The synthesised nanoparticles were characterised using scaning electron microscopy (SEM) and dynamic light scattering measurement (DLS). The sizes of the spherical silver particles were found to be in the range of 27–33 nm. The effect of silver ions concentrations on the synthesis of silver nanoparticles and average particle size was investigated. As-prepared Ag NPs had an excellent catalytic activity as a catalyst for the degradation of ibuprofen, which was carried out in 50 s. The current findings are equally extendable for safeguarding the aquatic environment against the pollution caused by drugs and microbial activity via a facile, highly economical, rapid and efficient reduction/degradation method based on the catalytic potential of Ag NPs. The report emphasizes the effect of the size of silver nanoparticles on the degradation rate of hazardous dyes - methyl blue by NaBH4..

scholarly journals Laser Ablated Silver Nanoparticles with Nearly the Same Size in Different Carrier Media

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Antonio M. Brito-Silva ◽  
Luiz A. Gómez ◽  
Cid B. de Araújo ◽  
André Galembeck
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Chemical Reduction ◽  
Average Particle Size ◽  
Good Control ◽  
Silver Ions ◽  
Vinyl Pyrrolidone ◽  
Poly Vinyl Alcohol ◽  
Average Particle ◽  
Resonance Band

Poly(vinyl-pyrrolidone) (PVP) stabilized silver nanoparticles with an average particle size ranging from 4.3 to 4.9 nm were synthesized by laser ablation in preformed colloids in methanol, acetone, ethylene glycol, and glycerin. Aqueous colloids obtained using PVP, poly(vinyl-alcohol) (PVA), and sodium citrate as stabilizing agents also lead to a good control over particle size distribution. Silver ions were reduced with sodium borohydride. The smaller average particle size and narrower dispersivity in comparison to previously reported data were ascribed to the relatively small size of the particles formed in the chemical reduction step, laser fluence, and the use of PVP, which was not previously used as the stabilizing agent in “top-down” routes. The surface plasmon resonance band maximum wavelength shifted from 398 nm in methanol to 425 nm in glycerin. This shift must be due to solvent effects since all other variables were the same.

The Aggregation Study and Characterization of Silver Nanoparticles

2017 ◽  
Vol 263 ◽  
pp. 165-169
Author(s):  
Silvia Chowdhury ◽  
Faridah Yusof ◽  
Nadzril Sulaiman ◽  
Mohammad Omer Faruck
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Particle Size ◽  
Silver Nanoparticles ◽  
Absorption Spectrum ◽  
Average Particle Size ◽  
Average Particle ◽  
Transmission Electron ◽  
Vis Spectroscopy

In this article, we have studied the process of silver nanoparticles (AgNPs) aggregation and to stop aggregation 0.3% Polyvinylpyrrolidone (PVP) was used. Aggregation study carried out via UV-vis spectroscopy and it is reported that the absorption spectrum of spherical silver nanoparticles were found a maximum peak at 420 nm wavelength. Furthermore, Transmission Electron Microscopy (TEM) were used to characterized the size and shape of AgNPs, where the average particle size is around 10 to 25 nm in diameter and the AgNPs shape is spherical. Next, Dynamic Light Scattering (DLS) were used, owing to observed size distribution and self-correlation of AgNPs.

scholarly journals Biosintesis Nanopartikel Perak Menggunakan Ekstrak Metanol Daun Kemangi (Ocimum Citriodorum)

2017 ◽  
Author(s):  
Yusnita Rifai
Keyword(s):  
Silver Nanoparticles ◽  
Reduction Method ◽  
Average Particle Size ◽  
Mechanical Effect ◽  
Average Particle ◽  
X Ray Diffraction ◽  
Cubic Crystal ◽  
X Ray ◽  
Speed Up ◽  
And Storage

AbstrakNanopartikel perak telah disintesis menggunakan metode reduksi. Dalam penelitian ini, ekstrak metanol daun Kemangi (Ocimum citriodorum) digunakan sebagai agen pereduksi untuk prekursor AgNO3. Sintesis nanopartikel perak dilakukan dengan mencampurkan laru- tan AgNO3 1mM dengan filtrat ekstrak daun kemangi. Hasil karakterisasi UV-Vis menun- jukkan bahwa nilai absorbansi meningkat dengan meningkatnya waktu kontak reaksi. Pun- cak absorbansi spektrum UV-Vis dari sampel biosintesis nanopartikel perak berkisar pada 427-439 nm selama 1 hari dengan pengadukan dan penyimpanan. Ukuran nanopartikel perak ditentukan menggunakan Pengukur Ukuran Partikel (PSA) dengan rata-rata distribusi uku- ran partikel sebesar 57,38 nm. Efek mekanik dalam proses biosintesis nanopartikel perak cenderung mempercepat pembentukan nanopartikel perak. Hasil karakterisasi menggunakan Difraksi Sinar-X (XRD) diketahui kristalit yang terbentuk memiliki intensitas terbesar pada sudut 38° dengan nilai FWHM 0,66310 (ukuran 0,3 nm) dalam sistem kristal kubik.Kata kunci: Biosintesis, Nanopartikel Perak, Ocimum citriodorum, Karakterisasi AbstractSynthesis of silver nanoparticles by using the reduction method with methanol extract basil (Ocimum citriodorum) leaves, which acted as a reducing agent for AgNO3 precursor have been conducted. Synthesis nanoparticles was carried out by mixing the solution of AgNO3 1mM with filtrate extract of Ocimum leaves. The results of characterization showed that absorbance values increased with the increase in reaction time. Peak of UV-Vis absorption spectrum of biosynthesis sample of silver nanoparticles with stirring and storage each at a wavelength 427-439 nm for 1 day. Silver nanoparticles size was determined by using PSA (Particles Size Analyzer) with an average particle size distribution of 57,38 nm. Mechanical effect in biosynthesis process of silver nanoparticles tends to speed up the formation of silver nanoparticles. The result of characterization by using X-Ray Diffraction (XRD) described that the formed crystal had the angle of 38° with the value of FWHM 0,66310 (sixe 0.3 nm) in cubic crystal system.Key word: Biosynthesis, Silver Nanoparticles, Ocimum citriodorum, Characterization.

scholarly journals Study of Antibacterial Properties of Ziziphus mauritiana based Green Synthesized Silver Nanoparticles against Various Bacterial Strains

2020 ◽  
Vol 12 (4) ◽  
pp. 1484 ◽  
Author(s):  
M. Asimuddin ◽  
Mohammed Rafi Shaik ◽  
Neeshat Fathima ◽  
M. Shaistha Afreen ◽  
Syed Farooq Adil ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Low Cost ◽  
Preliminary Investigation ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Ziziphus Mauritiana ◽  
Bacterial Strains ◽  
Vis Spectroscopy ◽  
Ft Ir

Due to their low cost and environmentally friendly nature, plant extracts based methods have gained significant popularity among researchers for the synthesis of metallic nanoparticles. Herein, green synthesis of silver nanoparticles was performed using the aqueous solution of Ziziphus mauritiana leaves extract (ZM-LE) as a bio-reducing agent. The as-obtained silver nanoparticles were characterized by using UV-Vis spectroscopy, XRD (X-ray diffraction), TEM (transmission electron microscopy), and FT-IR (Fourier-transform infrared spectroscopy). In addition, the effects of the concentrations of the leaves extract, silver nitrate, and the temperature on the preparation of nanoparticles were also investigated. In order to determine the nature of secondary metabolites present in leaves extract, a preliminary investigation of phytoconstituents was carried out using different methods including Folin-Ciocalteu and AlCl3 methods. The results have indicated the presence of a considerable amount of phenolic and flavonoid contents in the leaves extract, which are believed to be responsible for the reduction of silver ions and stabilization of resulting nanoparticles. Indeed, the FT-IR spectrum of silver nanoparticles also confirmed the presence of residual phytomolecules of leaves extract as stabilizing ligands on the surface of nanoparticles. The antibacterial properties of as-obtained silver nanoparticles were tested against various bacterial strains including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis. The nanoparticles strongly inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 2.5 μg/ml and moderately inhibited the growth of E. coli with a MIC of 5 μg/ml.

scholarly journals Influence of Cr2O3 Nanoparticles on the Structural, Optical, Thermal and Electrical Properties of PEO/CMC Nanocomposites

2021 ◽  
Author(s):  
Laila Gaabour
Keyword(s):  
Energy Gap ◽  
Average Particle Size ◽  
Nanocomposite Films ◽  
Average Particle ◽  
Cole Plot ◽  
Optical Energy Gap ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Cr2o3 Nanoparticles ◽  
A Charge

Abstract In the present paper, different concentrations of chromium oxide (Cr2O3) nanoparticles were incorporated within PEO/CMC polymer blend to produce nanocomposite films using the casting method. The X-ray diffraction was performed on PEO/CMC-Cr2O3 nanocomposites. The main X-ray peaks of Cr2O3 were observed and defined as cubic structure and orthorhombic shape with an average particle size of the Cr2O3 ~ 50-80 nm. The decrease of some IR bands after the addition of Cr2O3 nanoparticles was found attributed to the interactions between PEO/CMC and Cr2O3. Effect of Cr2O3 nanoparticles on optical properties such as absorbance and optical energy gap (Eg) were characterized using UV-Vis spectroscopy. The Eg was reduced after the addition of Cr2O3 nanoparticles. The AC conductivity (sac), dielectric constant (ε′), dielectric loss (ε′′) and the dielectric modulus (M′ and M′′) were calculated at frequency range 0.1 Hz-7 GHz. The increases of direct conductivity (σdc) imply that the free charge density or of the charge mobility that results. The estimated values of both ε′ and ε′′ were decreased with increases of frequency. The addition of Cr2O3 nanoparticles causes the formation of a charge-transfer complex. The Cole-Cole plot between (M′ and M′′) shows a semi-circular shape confirm discuses according to a non‐Debye method.

Biosynthesis of Silver Nanoparticles Using Plant Extract and its Anti-Plasmodial Property

2015 ◽  
Vol 1086 ◽  
pp. 11-30 ◽  
Author(s):  
Chellasamy Panneerselvam ◽  
Kadarkarai Murugan ◽  
Duraisamy Amerasan
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Plant Extract ◽  
Metallic Nanoparticles ◽  
Antimicrobial Properties ◽  
Nanoparticle Synthesis ◽  
Silver Ions ◽  
Antiplasmodial Activity ◽  
X Ray ◽  
Scanning Electron

Metallic nanoparticles have received great attention from chemists, physicists, biologists and engineers who wish to use them for the development of a new generation of nanodevices. In the present Communication, a completely “green” chemistry method for producing silver nanoparticles is introduced. The process is simple, environmentally benign, and quite efficient. Green nanoparticle synthesis has been achieved using environmentally acceptable plant extract and ecofriendly reducing and capping agents. In particular, silver nanoparticles are proved to have potential antibacterial, antifungal and antiplasmodial and antimicrobial properties. The present study was aimed to identify the antiplasmodial activity of green synthesised silver nanoparticles (AgNPs) using aqueous extract of plantEuphorbia hirtaagainstP.falciparum. Nanoparticles are being used in many commercial applications. It was found that aqueous silver ions can be reduced by aqueous extract of plant to generate extremely stable silver nanoparticles in water. The bio-reduced silver nanoparticles were appropriately characterized by UV–vis spectrum, Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). The formation of the AgNPs synthesized from the XRD spectrum compared with the standard confirmed spectrum of silver particles formed in the present experiments were in the form of nanocrystals, as evidenced by the peaks at 2θ values of =28.01°, 32.41°, 46.44°, 55.05° and 57.75°. The scanning electron micrograph (SEM) showed structures of spherical, cubic shape, and the size range was found to be 30–60 nm. The EDX spectra showed the purity of the material and the complete chemical composition of the synthesized AgNPs. The parasitic inhibition was dose-dependent. The synthesized AgNPs showed considerable antiplasmodial activity than the crude methanol and aqueous leaf extract ofE.hirta. The maximum efficacy was

scholarly journals Facile and Green Route to Silver Nanoparticles Using Aqueous Plant Extract, and their Photocatalytic and Antibacterial Studies

2020 ◽  
Vol 26 (4) ◽  
pp. 489-497
Author(s):  
Elias Emenka ELEMIKE ◽  
Damian Cinedu ONWUDIWE ◽  
Tanzim SAIYED ◽  
Anthony Chinonso EKENNIA ◽  
Mayowa Akeem AZEEZ
Keyword(s):  
Silver Nanoparticles ◽  
Plant Extract ◽  
Average Particle Size ◽  
Photocatalytic Property ◽  
Silver Ions ◽  
Cost Effective ◽  
Average Particle ◽  
Welsh Onion ◽  
Characteristic Functional ◽  
Transmission Electron

Silver nanoparticles were prepared through an environmental friendly and cost-effective plant-mediated technique, using crude extracts of Welsh onion plant. The synthesized nanoparticles were characterized using UV-vis spectrophotometer, powdered X-ray diffractometer (p-XRD), Fourier transform infra-red (FTIR) spectrophotometer, and transmission electron microscope (TEM). Silver nanoparticles of different sizes and morphologies were obtained by varying some synthesis parameters such as concentrations of AgNO3 (1, 2 and 5 mM) and ratio of the volume of the plant extract to AgNO3 (1:5 and 1:10) at constant reaction temperature of 80 °C. The difference in the reaction conditions showed significant effects on silver nanoparticles obtained. The surface plasmon resonance (SPR) varied with change in concentration of AgNO3 and the ratio of the AgNO3 to the plant extracts. The lowest SPR appeared around 412 nm (2 mM; 1:10), while the largest was achieved around 427 nm (5 mM; 1:10). FTIR results revealed the presence of different characteristic functional groups responsible for the bioreduction of silver ions in Welsh onion extract. Transmission electron microscopy (TEM) showed that the lowest average particle size of the silver nanoparticles was 3.74 nm (2 mM; 1:10), while the highest was 15.72 nm (1 mM; 1:5). Monodispersed spherical shaped nanoparticles were obtained from the 2 mM concentration of the AgNO3, while particles with some degree of agglomeration were obtained from 1 and 5 mM concentration. The p-XRD studies revealed face centred cubic structures. The nanoparticles obtained from 1 and 5 mM (1:5) gave moderate photo-catalytic potentials in the degradation of methyl red dye. However, the photocatalytic property increased with increase in the concentration of the precursor salt (AgNO3) from 1 to 5 mM. Gram positive Staphylococcus aureus and Bacillus cereus and Gram negative Klebsiela pneumonia and Escherichia coli bacteria strains were susceptible to the silver nanoparticles (2 mM). The nanoparticles were most active against E. coli with a minimum inhibitory concentration (MIC) below 0.05 mg/mL. The silver nanoparticles could become potential compounds in the future antibiotic research.

scholarly journals Sargassum Influx on the Mexican Coast: A Source for Synthesizing Silver Nanoparticles with Catalytic and Antibacterial Properties

2021 ◽  
Vol 11 (10) ◽  
pp. 4638
Author(s):  
Jose Luis López-Miranda ◽  
Rodrigo Esparza ◽  
Marlen Alexis González-Reyna ◽  
Beatriz Liliana España-Sánchez ◽  
Angel Ramon Hernandez-Martinez ◽  
...  
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Average Particle Size ◽  
Antibacterial Properties ◽  
Average Particle ◽  
X Ray Diffraction ◽  
X Ray ◽  
Experimental Parameters ◽  
First Time

This work reports, for the first time, the synthesis of silver nanoparticles using extracts of the species of Sargassum natans and Sargassum fluitans (AgNPs-S). Their antibacterial and catalytic properties are compared with silver nanoparticles obtained by chemical synthesis (AgNPs-C). The characterization of AgNPs-S and AgNPs-C was carried out using ultraviolet–visible spectroscopy (UV–Vis), dynamic light scattering (DLS), zeta potential, a scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis. The synthesis of silver nanoparticles using Sargassum extract was optimized through varying experimental parameters, such as the type of solvent used to prepare the extract, the volume of the extract, and the pH of the system. The most efficient sample (AgNPs-S) was prepared with a water–ethanol-based extract, using a 3:1 volumetric ratio of extract: a precursor salt with the addition of 1 mL of NaOH pH = 14. The AgNPs-C were spherical in shape, with an average particle size of 11.55 nm, while the AgNPs-S were polyhedral shaped, with an average particle size of 26.39 nm. The synthesized AgNPs-S were found to have significantly higher catalytic activity for the degradation of methylene blue and more effective antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa than AgNPs-C.

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