scholarly journals A Novel Method to Overcome Coat-Imposed Seed Dormancy in Lupinus albus L. and Trifolium pratense L.

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Iskender Tiryaki ◽  
Mustafa Topu
Keyword(s):  
Seed Dormancy ◽  
Trifolium Pratense ◽  
Germination Rate ◽  
Lupinus Albus ◽  
Red Clover ◽  
Treatment Method ◽  
Germination Percentage ◽  
Hot Water ◽  
White Lupin ◽  
Novel Method

We have developed a novel method to overcome coat-imposed seed dormancy in legume plants. Seeds of Lupinus albus L. and Trifolium pratense L. were stored in a freezer at −80°C for a period of time and then immediately treated with or without hot water at 90°C for 5 seconds. Germination tests were carried out in darkness at 20±1.0°C with four replications in a completely randomized design. Final germination percentage (FGP), germination rate, and synchrony of seeds were evaluated. The results showed that new approach of freeze-thaw scarification provided high percentage of germinations in white lupin (84.16%) and red clover (74.50%) seeds while control seeds had FGPs of 3.3% and 26.0%, respectively. The immediate thawing of frozen seeds in hot water for 5 seconds was found not only an effective and reliable but also the quickest seed treatment method to prevail against coat-imposed seed dormancy in legume species and may become operationally applicable to other plant species.

scholarly journals Pengaruh Perlakuan Pencelupan Dan Perendaman Terhadap Perkecambahan Benih Sengon (Paraserianthes falcataria L.)

Agrologia ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Marthen Marthen ◽  
Elizabet Kaya ◽  
Herman Rehatta
Keyword(s):  
Seed Dormancy ◽  
Seed Coat ◽  
Cold Water ◽  
Germination Rate ◽  
Germination Percentage ◽  
Hot Water ◽  
Hard Seed ◽  
Randomized Design ◽  
Vigor Index ◽  
Two Factors

In developing productive forests, seeds play a crucial role. The seeds of forests tree have physical condition of hard seed coat, especially Leguminoceae family. Silvicultural techniques that can overcome seed coat dormancy are dyeing and submersion of seeds. The knowledge and experience in breaking seed dormancy of Sengon (Paracerianthes falcataria L.) is rare, therefore it is necessary to study how to overcome seed dormancy. The objective of this study was to determine the effect of dyeing and submersion treatments to overcome seed dormancy of Sengon seed to improve its viability. This research used Completely Randomized Design (CRD) with two factors: namely Seed (Factor A), dipped in hot water at 60 º C for (A0 = Control, A1 = 2 minutes, A2 = 4 minutes, A3 = 6 minutes), Factor B, seeds soaked in cold water: (B0 = control, B1 = 6 hours of soaking, B2 = 12 hours, B3 = 18 hours, B4 = 24 hours). The parameters observed were germination percentage, germination rate and vigor index. The results showed that among all parameters observed the interaction of factors A1B2, A2B2 and A3B2 were the best treatment for germination percentage which was equal to 100 percent, the best germination rate of 3.897 days and the best vigor index of 27.02

scholarly journals THE EFFECT OF THREE PRETREATMENTS ON BREAKING SEED DORMANCY OF BAOBAB (ADANSONIA DIGITATA L.)

2021 ◽  
pp. 1-3
Author(s):  
BASHIR KA ◽  
MUSA DD ◽  
BISHIR R
Keyword(s):  
Biological Sciences ◽  
Seed Dormancy ◽  
Germination Rate ◽  
Germination Percentage ◽  
Hot Water ◽  
Limiting Factor ◽  
Adansonia Digitata ◽  
Endangered Tree ◽  
Randomized Design ◽  
Pre Treatment

Objective: Baobab (Adansonia digitata) is a native tree that is found in African savannas, it is a member of family ‘Malvaceae’. The tree is found in Nigeria, South Africa, Botswana, Namibia, Mozambique and in different regions of western Madagascar. Seed dormancy is the most limiting factor for some plants propagation. Dormancy allows seeds to separate from their mother plant and survive dispersal, over distance, and time before growth recommences. This study was aimed to determine the effect of three pre-treatments on breaking the seed dormancy of Adansonia digitata L., a multi-purpose and indigenously endangered tree. Methods: The study was carried out in the greenhouse of the Department of Biological Sciences, Federal University Dutsin-Ma, Nigeria. The three pre-treatments are: Soaking in hot water (100°C) for 5, 10, 15, and 20 min; and soaking in sulfuric acid (H2SO4) and hydrochloric acid (HCL) concentrations (100%, 75%, 50%, and 25%) for 5, 10, 15, and 20 min. Untreated seeds served as control. These were laid out in a complete randomized design with three replications. Data were collected on germination percentage, germination rate, day of emergence, and the average seedlings height. Results: The result showed that earliest mean days of emergence (7 days) were observed in seeds subjected to hot water treatment at 15 min. It also showed the highest rate of germination and percentage 6 (100%). On the other hand, the effect of acids (H2SO4 and HCL) pre-treatments on the seeds gave a fair result (16.66–33.33%), untreated seeds did not germinate. Conclusion: All the pre-treatments used in the study were found to be effective. However, soaking of seeds in hot water (100°C) pre-treatment for 15 min is recommended for breaking the seed dormancy of A. digitata.

scholarly journals The Effects of Red Clover Seed Treatment with Cold Plasma and Electromagnetic Field on Germination and Seedling Growth Are Dependent on Seed Color

2021 ◽  
Vol 11 (10) ◽  
pp. 4676
Author(s):  
Anatolii Ivankov ◽  
Rasa Zukiene ◽  
Zita Nauciene ◽  
Laima Degutyte-Fomins ◽  
Irina Filatova ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Cold Plasma ◽  
Trifolium Pratense ◽  
Root Nodules ◽  
Germination Rate ◽  
Red Clover ◽  
Seed Color ◽  
Seed Treatments ◽  
Capacitively Coupled ◽  
Clover Seed

This study aimed to estimate the effects of cold plasma (CP) and electromagnetic field (EMF) treatment of red clover (Trifolium pratense) seeds with different coat colors on germination kinetics, the content of seed phytohormones, and the growth of seedlings. Seeds of red clover cultivar ‘Arimaiciai’ were treated with radio-frequency EMF or capacitively coupled low-pressure CP for different durations. There were no differences in germination kinetics between yellow, brown, and dark purple seeds in control, but the germination rate of seeds treated with CP and EMF depended on seed color: The germination of yellow seeds was stimulated stronger compared to dark purple and brown seeds, and EMF did not stimulate germination in brown seeds. The content of phytohormones in control seeds and the shift in their amount induced by seed treatments were also strongly dependent on seed color. No relationship was found between the effect on germination kinetics and changes in phytohormone levels. In the control, seedlings growing from the yellow seeds were heavier, and the number of root nodules was 12.5 times larger compared to seedlings of dark purple seeds. Seed treatments with CP and EMF significantly increased the number of root nodules, and this effect was stronger in seedlings from dark purple seeds compared to those from yellow seeds.

scholarly journals GA3 Assisted Seed Dormancy Breaking in Abelmoschus moschatus Medik. subsp. moschatus

2016 ◽  
Vol 49 (4) ◽  
pp. 27-34
Author(s):  
G. Gogoi ◽  
P.K. Borua ◽  
D. Chetia
Keyword(s):  
Seed Dormancy ◽  
Exothermic Reaction ◽  
Germination Percentage ◽  
Hot Water ◽  
Dormancy Breaking ◽  
Triphenyltetrazolium Chloride ◽  
Physiological Factor ◽  
Lady’S Finger ◽  
High Degree ◽  
Physical And Chemical

Abstract Abelmoschus moschatus Medik. subsp. moschatus is a wild uncultivated variety of common lady’s finger (Abelmoschus esculentus) possessing a high degree of seed dormancy. Methods of dormancy breaking in the seed of the plant were investigated through different physical and chemical methods. Different preconditioning treatments including hot water, dry heat, physical scarification and chemical treatments including exogenous GA3 were applied to explore the initiation in germination. Seeds were germinated under the controlled photoperiod and temperature. The viability of the test seeds was estimated by topographical 2, 3, 5-Triphenyltetrazolium chloride (TTC) solution test. Highest germination percentage was obtained in the seeds treated with the exothermic reaction of H2SO4 and H2O, followed by exposure to gibberellic acid (GA3). The exposure time of exogenously applied GA3 had a significant influence on the germination response. The optimum germination temperature was found to be 30 ± 0.5°C. Treatment with 0.75% of 2, 3, 5-Triphenyltetrazolium chloride solution for 4 h at 35 ± 0.5°C enabled to correlate the viability of the seeds with the germinative values. Maximum germination was induced through this technique and dormancy of the seed can be attributed due to hard impermeable seed coat and endogenous physiological factor.

scholarly journals (205) Germination of Echinacea Species Is Enhanced by ACC

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1069C-1069
Author(s):  
Laura A. Wood ◽  
Sharon Kester ◽  
Robert L. Geneve
Keyword(s):  
Seed Dormancy ◽  
North American ◽  
Germination Rate ◽  
Germination Percentage ◽  
Additional Work ◽  
Germination Speed ◽  
Viable Method

Echinacea are North American members of the Asteraceae, and all can show some degree of endogenous physiological seed dormancy that is alleviated by chilling stratification. In some species, ethephon has been shown to substitute for chilling stratification to relieve dormancy. The objective of this research was to investigate the effect of ACC on dormancy and germination in five Echinacea species. Germination for each species was 90%, 59%, 99%, 81%, and 21%, respectively. Germination on 5 mM ACC improved germination in E. tennesseensis, E. paradoxa, and E. simulata to 82%, 99%, and 82%, respectively, but there was no change for E. purpurea and E. angustifolia. Germination rate was dramatically accelerated in all species in the presence of ACC. On average, there were 57% more seeds germinated on ACC after 3 days compared to untreated seeds. Exposing E. purpurea and E. tennesseensis seeds to 1 or 2 days of 5 mM ACC before drying and subsequently re-hydrating the seeds did not have the same effect as continual exposure to ACC. Similarly, there was no clear enhancement of adding ACC during stratification over the improvement gained by chilling stratification alone. Seeds produced more ethylene upon germination following both stratification and ACC treatment. However, significantly more ethylene is produced during germination in ACC-treated seeds. It was clear that ACC-treated seeds showed improvement for enhanced germination speed and in some cases germination percentage. Unfortunately, this enhanced germination was not retained in seeds treated with ACC and dried prior to germination. Additional work is required to develop a commercially viable method of loading ACC into seeds for germination enhancement.

scholarly journals In-vitro Seed Germination Biology of Milicia Excelsa Population Collected from Benchi-Maji Zone, South Western Ethiopia

2020 ◽  
Author(s):  
MOHAMMED ADEFA SEID ◽  
Yigardu Mulatu ◽  
Agena Anjulo ◽  
Semaigzer Ayalew ◽  
Marshet Nigatu ◽  
...  
Keyword(s):  
Seed Germination ◽  
Tap Water ◽  
Germination Rate ◽  
Germination Percentage ◽  
Hot Water ◽  
Laboratory Conditions ◽  
Southwestern Ethiopia ◽  
Micro Propagation ◽  
Milicia Excelsa

Abstract Background: In Ethiopia, species used for timber production are limited to few species. So, it is becoming necessary to find innovative ways of maintaining and improving the genetic quality of species such as Milicia excelsa by domesticating them into a wider scale of ecological and social environment. Hence, the main objective of this study was to investigate the seed germination biology and behavior of Milicia excelsa under in-vitro laboratory conditions so as to promote the domestication and establishment of seed production areas (SPA) in-situ. Methods: Seeds collected from Benchi district of Benchi-Maji zone of southwestern Ethiopia were subjected to germination biology in-vitro to determine the germination responses of M. excelsa seeds under laboratory conditions. So, a total of 1200 seeds were randomly selected, treated and prepared using four treatments procedures with six replications for paper method and 3 replications for sand method, and tested using paper methods in CRD design. So, 600 seeds were tested using paper and sand methods each where 25 seeds were tested for each replication in paper and 50 seeds for each replication in sand method. The germination process was lasted between 19 March 2020 and 15 May 2020. Result: The mean germination percentage of seeds of M. excelsa was noticed to be lower than 20%. This is below the expected (45%) germination rate of mature and healthy seed lot. Overall, seeds that were not treated using any pre-sowing treatments has shown relatively better germination percentage (19%) followed by seeds rubbed by hand (15%). Analysis of one-way t-test shows that the effects untreated (control –T1) and rubbing by hand treatments (T4) is significant at p < 0.05 compared to the effects of the other pre-sowing treatments (T2- seeds only washed with normal tap water, and T3- seeds washed and soaked in hot water for 15 minutes) on the germination capability of seeds of M. excelsa . However, no significance difference was observed between control (T1) and hand scarification (T2). Conclusions: The average germination of M. excelsa seeds combined for all treatments is lower than 20% which is lower germination when comparing with other similar research reported in other countries. Therefore, it required to include and test other pre-sowing treatments including tissue culture micro-propagation of seeds to maximize the germination of M. excelsa seeds helping to get enough seedlings required for the domestication project.

scholarly journals EFFECT OF SEED PRIMING METHODS ON THE GERMINATION OF DETARIUM MICROCARPUM

2021 ◽  
pp. 1-10
Author(s):  
A Abdulrahman ◽  
M Atiku ◽  
A.S Ambursa ◽  
I Umar ◽  
M Muhammed ◽  
...  
Keyword(s):  
Tap Water ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Hot Water ◽  
State University ◽  
Detarium Microcarpum ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pre Treatment

This study was carried out at Kebbi State University of Science and Technology, Aliero, Kebbi State, to determine the effect of seed priming methods on the germination of Detarium microcarpum. The study was carried out in the Forestry Laboratory of the Faculty of Agriculture. The effectiveness of nine (9) different seed priming methods was assessed, control inclusive. Treatment 1 (TRT1) seeds were soaked in tap water for 48 hours, Treatment 2 (TRT2) seeds were mechanically scarified opposite to microphyle, treatment 3, 4, and 5 (TRT3, TRT4, TRT5) seeds were immersed in H2SO4 for 5, 10 and 15 minutes respectively, treatment 6, 7 and 8 (TRT6, TRT7 and TRT8) seeds were soaked in hot water (100oC) for 5, 10 and 15 minutes respectively and untreated seeds (CNTRL). Completely Randomized Design with three (3) replications was used. The data obtained were analyzed using one-way Analysis of Variance (ANOVA).The result revealed that seeds of D. microcarpum subjected to TRT2, TRT4, TRT5 and TRT8 recorded with highest Final Germination Percentage (FGP 100%) and was found to differ significantly (P<0.05) with other treatment and CNTRL recorded the lowest FGP (60%).Thus, TRT2 was significantly (P<0.05) enhanced the seed and germination rate of D. microcarpum with the highest FGP (100%), lowest MGT (6.67), highest GRI (15.05), highest GE (12.01), lower FDG (5.67) with maximum GI value (900). Based on the result, pre-treatment of T. indica seeds with H2SO4 is recommended for effective germination.

Factors controlling seed dormancy and germination response of Brachypodium hybridum growing in the hot arid mountains of the Arabian Desert

Botany ◽  
2019 ◽  
Vol 97 (7) ◽  
pp. 371-379 ◽  
Author(s):  
Masarra Elgabra ◽  
Ali El-Keblawy ◽  
Kareem A. Mosa ◽  
Sameh Soliman
Keyword(s):  
Seed Dormancy ◽  
Light Quality ◽  
Genetic Model ◽  
Germination Rate ◽  
Red Light ◽  
Germination Percentage ◽  
Light Conditions ◽  
One Year ◽  
Native And Introduced Ranges ◽  
Seed Dormancy And Germination

Seed dormancy and germination have been studied in the genetic model Brachypodium species complex in cooler, moist higher latitudes. Studying environmental factors in arid mountains affecting dormancy and germination in Brachypodium complex could determine the factors controlling these processes. This study assesses the impacts of temperature during seed maturation, seed after-ripening, drought, photoperiod, and thermoperiod on final germination and germination rate index of B. hybridum in the Arabian Desert. Seeds were germinated under dark or light conditions and under different ratios of red:far-red light, with three diurnal thermoperiods. The final germination percentage was significantly greater at 15/25 °C and 20/30 °C than at 25/35 °C and in light rather than in darkness. Seeds that reached maturity at 15/25 °C attained greater germination rates and faster germination than those that reached maturity at 20/30 °C. One-year after-ripening enhanced the final germination percentage and reduced photoperiod requirements. Light quality did not affect final germination percentage. The seeds tolerated drought of up to –0.8 MPa polyethylene glycol. The tolerance of B. hybridum seeds produced at higher thermoperiods to moderate levels of osmotic stress and their higher dormancy indicate that this species has the potential to survive the projected global warming in its native and introduced ranges.

scholarly journals Effect of Pre-Sowing Treatments on Seed Germination and Seedling Growth of Silver Butterfly Tree (Bauhinia rufescens)

2018 ◽  
Vol 6 (3) ◽  
pp. 344-354
Author(s):  
J. A. Opoku ◽  
J. N. Amissah ◽  
M. E. Essilfie ◽  
J. C. Norman
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Growth Regulator ◽  
Tap Water ◽  
Germination Rate ◽  
Germination Percentage ◽  
Hot Water ◽  
Root Weight ◽  
Number Of Leaves ◽  
Shoot Weight

Three experiments were conducted in a plastic greenhouse at the Sinna Garden of the Crop Science Department of the University of Ghana, Legon, Accra to investigate into pre-sowing treatment (water treatment, growth regulator, and sulphuric acid scarification and water soaking) effects on seed germination and seedling growth of Bauhinia rufescens from October to December, 2011 and January to April, 2012, respectively. The experimental design used was complete randomized design. There were 4 treatments in experiment 1, 7 in experiment 2 and 6 in experiment 3. Each experiment was replicated five (5) times. The results showed that Bauhinia rufescens seeds soaked in hot water at 65 0 C for 60 minutes gave the highest mean germination rate, tallest plant height and highest number of leaves per plant at 49 days after soaking. It also had the widest stem diameter, highest fresh shoot and root weight, highest fresh root and dry root weight and number of roots per seedling. Seeds soaked in 500 ppm Gibberelic acid(GA3) produced the highest number of leaves per plant and tallest plants at 49 DAS. Seeds soaked in 750 ppm GA3 had the highest number of leaves per plant at 49 DAS and highest fresh shoot weight. Seeds soaked in 1000 ppm Promalin differed significantly from 500 ppm GA3 and the control in germination percentage. Seeds soaked in 1000 ppm Promalin differed significantly from 500 ppm GA3 and the control in germination percentage. Acid scarified seeds for 60 minutes plus soaking in tap water for 24 hours differed significantly from the seeds treated withother growth regulator rates plus 24 hours soaking in germination percentage. Acid scarified seeds for 45 minutes plus soaking in tap water for 24 hours had the tallest plant and highest number of leaves per plant at 49 DAS, widest stem diameter, highest fresh shoot and root weight and dry shoot weight. Soaking seeds in hot water at 65 0 C for 60 minutes and/or in acid (H2SO4) for 45 minutes plus soaking in tap water for 24 hours is recommended as pre-sowing treatment for Bauhinia rufescens for maximum mean germination rate and vigorous vegetative growth of seedlings.

scholarly journals The effect of different treatments on seed dormancy breaking and germination inducing in Louisiana variety of Okra (Abelmoschus esculentus L.)

2020 ◽  
Vol 3 (4) ◽  
pp. 124-128
Author(s):  
Ali Jawed Safdary ◽  
Ahmad Jawid Ahamdi ◽  
Nasratullah Habibi ◽  
Zabiullah Rahmani ◽  
Saifullah Rasooli
Keyword(s):  
Hydrochloric Acid ◽  
Gibberellic Acid ◽  
Seed Dormancy ◽  
Germination Rate ◽  
Cold Treatment ◽  
Winter Season ◽  
Dry Weight ◽  
Hot Water ◽  
Control Treatment ◽  
Dormancy Breaking

To investigate the methods of dormancy breaking in okra seeds; a study was conducted in the winter season of 2019 under laboratory conditions in Samangan Higher Education Institute. The experiment was carried out based on a completely randomized design within 10 treatments and 3 replications. The treatments included gibberellic acid at 4 levels (100, 500, 700, and 1000 ppm), hot water (70°C), hydrochloric acid (75%) at three levels (5, 10, and 15 minutes), 7-day chilling at 3°C and control treatment (distilled water). The results showed that the highest percentage (100%) with a germination rate of 86 seeds were available due to the application of 7-day cold treatment. Moreover, the treatments such as 7-day chilling, gibberellic acid 500 ppm, and hydrochloric acid 10 and 15 minutes had significant effects on the fresh and dry weight of roots and shoots as well as on their length. we observed that the 7- day cooling at 3°C is the best and most appropriate solution to break down the seed dormancy and stimulate seed germination of the above-mentioned crop.

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