scholarly journals Effect of Sodium Carbonate Concentrations on the Formation and Mechanism of Regenerated Silk Fibroin Nanofibers by Electrospinning

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Hao Dou ◽  
Baoqi Zuo
Keyword(s):  
Rheological Property ◽  
Aqueous Solutions ◽  
Silk Fibroin ◽  
Sodium Carbonate ◽  
Electrospinning Process ◽  
Carbonate Concentration ◽  
Low Viscosity ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Nanofibers ◽  
First Time

Degumming is the first process for the preparation of all silk-based products. In this paper, effect of sodium carbonate concentrations for silk degumming on the formation of electrospun silk fibroin nanofibers was investigated and the reason for the silk electrospinning process was explained for the first time by differences from the microstructure of regenerated silk fibroin. With increasing the sodium carbonate concentration, microstructure both in the aqueous solutions and in the electrospinning solutions transformed from nanofibrils to nanoparticles, leading to obvious changes on rheological property; electrospinning solutions with nanofibrils behaved like the native silk dope and owned remarkably higher viscosity than the solutions with nanoparticles showing very low viscosity. More interestingly, nanofibrils favored the formation of silk nanofibers with ease, and even nanofibers could be electrospun at concentration 2%. However, nanoparticles were completely unable to generate nanofibers at high spinning concentration 8%. Importance of sodium carbonate concentrations is heavily emphasized for impacting the microstructure types and further influencing the electrospinning performance of regenerated silk. Hence, sodium carbonate concentrations provide a controllable choice for the preparation of silk-based electrospun biomaterials with desired properties.

scholarly journals Effect of Na2CO3 degumming concentration on LiBr-formic acid-silk fibroin solution properties

2016 ◽  
Vol 20 (3) ◽  
pp. 985-991
Author(s):  
Zhi Liu ◽  
Yuqin Wan ◽  
Hao Dou ◽  
Ji-Huan He
Keyword(s):  
Formic Acid ◽  
Silk Fibroin ◽  
Sodium Carbonate ◽  
High Efficiency ◽  
Lithium Bromide ◽  
Solution Properties ◽  
Acid System ◽  
Acid Dissolution ◽  
Carbonate Concentration ◽  
Degumming Process

Salt-acid system has been proved to be of high efficiency for silk fibroin dissolution. Using salt-acid system to dissolve silk, native silk fibrils can be preserved in the regenerated solution. Increasing experiments indicate that acquirement of silk fibrils in solution is strongly associated with the degumming process. In this study, the effect of sodium carbonate degumming concentration on solution properties based on lithium bromide-formic acid dissolution system was systematically investigated. Results showed that the morphology transformation of silk fibroin in solution from nanospheres to nanofibrils is determined by sodium carbonate concentration during the degumming process. Solutions containing different silk fibroin structure exhibited different rheological behaviors and different electrospinnability, leading to different electrospun nanofibre properties. The results have guiding significance for preparation and application of silk fibroin solutions.

scholarly journals Adsorption/desorption and biofunctional properties of oleuropein loaded on different types of silk fibroin matrices

2017 ◽  
Vol 36 (1) ◽  
Author(s):  
Oguz Bayraktar ◽  
Ali Bora Balta ◽  
Guldemet Basal Bayraktar
Keyword(s):  
Silk Fibroin ◽  
Antibacterial Properties ◽  
Average Diameter ◽  
Electrospinning Technique ◽  
Collector Plate ◽  
Regenerated Silk Fibroin ◽  
Different Types ◽  
Healing Property ◽  
Silk Fibroin Nanofibers ◽  
Adsorption Desorption

The objective of this study was to investigate the adsorption/desorption behavior of oleuropein on different types of silk fibroin matrices including silk fibroin microfibers (MF), regenerated silk fibroin (RSF), and silk fibroin nanofibers (NF). Nanofibers with an average diameter of ranging between 24 and 326 nm were successfully prepared using the electrospinning technique. The effects of the silk fibroin concentration, the voltage applied and the distance between needle tip and collector plate on the morphology of the NF were investigated. The adsorption capacities of MF, RSF and NF were determined as 104.92, 163.07 and 228.34 mg oleuropein per gram of material, respectively. The percentage of initially adsorbed oleuropein that was desorbed was 86.08, 91.29 and 96.67% for MF, RSF and NF, respectively.NF and RSF discs loaded with oleuropein were subjected to disc diffusion assays to determine their antibacterial activity against test microorganisms Staphylococcus epidermidis (Gram +) and Escherichia coli (Gram – ). The results showed that both biomaterials possessed antibacterial properties after loading with oleuropein. Wound scratch assays using oleuropein released from NF revealed an enhancement of cell migration, indicating a wound healing property of the material.In conclusion, the NF can be utilized as a biofunctional polymeric material with better performance for the adsorption and desorption of oleuropein compared with MF and RSF.

Hybrid Silk Fibers Dry-Spun from Regenerated Silk Fibroin/Graphene Oxide Aqueous Solutions

2016 ◽  
Vol 8 (5) ◽  
pp. 3349-3358 ◽  
Author(s):  
Chao Zhang ◽  
Yaopeng Zhang ◽  
Huili Shao ◽  
Xuechao Hu
Keyword(s):  
Graphene Oxide ◽  
Aqueous Solutions ◽  
Silk Fibroin ◽  
Silk Fibers ◽  
Regenerated Silk Fibroin

Influence of shear on the structures and properties of regenerated silk fibroin aqueous solutions

RSC Advances ◽  
2015 ◽  
Vol 5 (77) ◽  
pp. 62936-62940 ◽  
Author(s):  
Yuan Jin ◽  
Yichun Hang ◽  
Qingfa Peng ◽  
Yaopeng Zhang ◽  
Huili Shao ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Shear Rate ◽  
Aqueous Solutions ◽  
Crystal Structures ◽  
Silk Fibroin ◽  
Structures And Properties ◽  
Regenerated Silk Fibroin

Regenerated silk fibroin molecules in aqueous solutions gradually form rod-like liquid crystal structures after being applied sufficient shear rate and shear time.

Effect of Solvent on the Characteristics of Electrospun Regenerated Silk Fibroin Nanofibers

2007 ◽  
Vol 342-343 ◽  
pp. 813-816 ◽  
Author(s):  
Lim Jeong ◽  
Kuen Yong Lee ◽  
Won Ho Park
Keyword(s):  
Secondary Structure ◽  
Formic Acid ◽  
Silk Fibroin ◽  
Structural Changes ◽  
Effective Means ◽  
Electrospun Nanofibers ◽  
Random Coil ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Nanofibers ◽  
Β Sheet

Nonwoven nanofiber matrices were prepared by electrospinning a solution of silk fibroin (SF) dissolved either in formic acid or in 1,1,1,3,3,3-hexafluoro-2-isopropyl alcohol (HFIP). The mean diameter of the electrospun nanofibers prepared from SF dissolved in formic acid was 80 nm with a unimodal size distribution, which was smaller than those prepared from HFIP (380 nm). SF nanofibers were then treated with an aqueous methanol solution, and structural changes due to solvent-induced crystallization of SF were investigated using IR and 13C solid-state CP/MAS NMR spectroscopy. SF nanofibers prepared from formic acid were found to have a higher proportion of β-sheet conformations than those prepared from HFIP. Methanol treatment provided a fast and effective means to alter the secondary structure of both types of SF nanofibers from a random coil form to a β-sheet form. As demonstrated in the present study, this approach to controlling the dimensions and secondary structure of proteins using various solvents may be useful for the design and tailoring of materials for biomedical applications, especially for tissue engineering applications.

Role of Ca2+on structures and properties of regenerated silk fibroin aqueous solutions and fibres

2014 ◽  
Vol 18 (sup2) ◽  
pp. S2-113-S2-115 ◽  
Author(s):  
Y. Jin ◽  
Y. C. Hang ◽  
Y. P. Zhang ◽  
H. L. Shao ◽  
X. C. Hu
Keyword(s):  
Aqueous Solutions ◽  
Silk Fibroin ◽  
Structures And Properties ◽  
Regenerated Silk Fibroin

Property Research of Silk Fibroin Nanofibers by Electrospinning Dissolved in CaCl2-Formid Acid

2015 ◽  
Vol 1120-1121 ◽  
pp. 331-336
Author(s):  
Yan Zhou ◽  
Hui Ying Wu
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Solvent System ◽  
Dissolution Time ◽  
Ethanol Treatment ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Nanofibers ◽  
Different Content ◽  
Β Sheet ◽  
Paper Property

In this paper, property research of silk fibroin nanofibers by electrospinning dissolved in CaCl2-Formid Acid was discussed. The dissolve process, morphology, structure and mechanical properties of fibers were studied. It showed that more dissolution time was needed to dissolve silk with 1% CaCl2. The diameters of silk fibroin nanofibers decreased with the CaCl2 contents increased and the surface of 6%5% fibers was more uniform and smooth. After ethanol treatment, strong peak at 1628cm-1, 1517cm-1 and 1231cm-1 was corresponding to β-sheet. It was important to discover that silk fibroin was weakly destroyed in CaCl2-FA solvent system, and the regenerated silk fibroin nanofibers had better mechanical properties. The stress of 6%5% fibers was nearly 3 times to traditional way. With different content of CaCl2, the stress of 6%5% fibers was nearly 2 times to 6%1% fibers.

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