scholarly journals Isolation of Insecticidal Constituents from the Essential Oil ofAgeratum houstonianumMill. againstLiposcelis bostrychophilaBadonnel

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Xiao Nan Lu ◽  
Xin Chao Liu ◽  
Qi Zhi Liu ◽  
Zhi Long Liu

The aim of this research was to determine chemical composition and insecticidal activities of the essential oil ofAgeratum houstonianumMill. aerial parts against booklice,Liposcelis bostrychophilaBadonnel, and to isolate any insecticidal constituents from the oil. Essential oil ofA. houstonianumwas obtained by hydrodistillation and analyzed by GC-MS. A total of 35 components in the essential oil were identified. The major compounds were precocene II (62.68%), precocene I (13.21%), andβ-caryophyllene (7.92%). Based on bioactivity-guided fractionation, precocene II and precocene I were isolated and identified as the active constituents. The essential oil exhibited contact toxicity againstL. bostrychophilawith an LC50value of 50.8 μg/cm2. Precocene II (LC50= 30.4 μg/cm2) exhibited stronger acute toxicity than precocene I (LC50= 64.0 μg/cm2) against the booklice. The essential oil and the two isolated constituents also possessed strong repellent activity againstL. bostrychophila. The results indicated that the essential oil and its constituent compounds have potential for development into natural insecticides or repellents for control of insects in stored grains.

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Xin Chao Liu ◽  
Ligang Zhou ◽  
Zhi Long Liu

The aim of this research was to determine chemical composition and insecticidal activities of the essential oil ofValeriana jatamansiJones roots against booklice,Liposcelis bostrychophilaBadonnel, and to isolate insecticidal constituents from the oil. Essential oil ofV. jatamansiwas obtained by hydrodistillation and analyzed by GC-MS. A total of 27 components in the essential oil were identified. The major compounds were patchoulol (24.3%),α-bulnesene (13.8%), isovaleric acid (12.9%),α-guaiene (8.7%), and 3-methylvaleric acid (8.4%). Based on bioactivity-guided fractionation, isovaleric acid, 3-methylvaleric acid, and patchoulol were isolated and identified as the active constituents. The essential oil exhibited contact toxicity againstL. bostrychophilawith an LC50value of 236.4 μg/cm2. Patchoulol (LC50= 61.35 μg/cm2) exhibited stronger acute toxicity than 3-methylvaleric acid (LC50= 210.69 μg/cm2) against the booklice. The essential oil also possessed fumigant toxicity againstL. bostrychophilawith an LC50value of 6.0 mg/L, while 3-methylvaleric acid and isovaleric acid had LC50values of 5.53 mg/L and 5.67 mg/L against the booklice, respectively. The results indicated that the essential oil and its constituent compounds have potential to develop into natural insecticides or fumigants for control of insects in stored grains.


2015 ◽  
Vol 78 (4) ◽  
pp. 772-777 ◽  
Author(s):  
XIN CHAO LIU ◽  
ZHI LONG LIU

Water-distilled essential oil from Illicium henryi (Illiciaceae) root bark was analyzed by gas chromatography–mass spectrometry. Thirty-four compounds, accounting for 97.86% of the total oil, were identified. The main components of the essential oil of I. henryi root bark were safrole (46.12%), myristicin (20.39%), and 1,8-cineole (6.17%), followed by α-cadinol (3.784%) and linalool (3.22%). The essential oil had higher levels of phenylpropanoids (66.89%) than of monoterpenoids (14.83%) and sesquiternoids (16.14%). Three constituents were isolated from the oil based on bioactivity fractionation. The essential oil possessed fumigant toxicity against booklice (Liposcelis bostrychophila), with a 50% lethal concentration (LC50) of 380.39 μg/liter of air, while the two isolated constituents myristicin and safrole had LC50s of 121.95 and 322.54 μg/liter, respectively. Another constituent, 1,8-cineole, showed weaker toxicity, with an LC50 of 1,120.43 μg/liter. The essential oil also exhibited contact toxicity against L. bostrychophila, with an LC50 of 96.83 μg/cm2. Myristicin (LC50, 18.74 μg/cm2) and safrole (LC50, 69.28 μg/cm2) exhibited stronger acute toxicity than 1,8-cineole (LC50, 1,049.41 μg/cm2) against the booklice. The results indicated that the essential oil and its constituent compounds have potential for development into natural insecticides for control of psocids in stored grains.


2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Xin Chao Liu ◽  
Xianghong Hao ◽  
Ligang Zhou ◽  
Zhi Long Liu

The roots ofEchinops latifoliusTausch (Asteraceae) have been used in the traditional medicine. However, no report on chemical composition and insecticidal activities of the essential oil of this plant exists. The aim of this research was to determine chemical composition and insecticidal activities of the essential oil ofE. latifoliusaerial parts against maize weevils (Sitophilus zeamaisMotschulsky) for the first time. Essential oil ofE. latifoliusaerial parts at flowering stage was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 35 components of the essential oil ofE. latifoliusaerial parts were identified. The major compounds in the essential oil were 1,8-cineole (19.63%), (Z)-β-ocimene (18.44%), andβ-pinene (15.56%) followed byβ-myrcene (4.75%) and carvone (4.39%). The essential oil ofE. latifoliuspossessed contact toxicity againstS. zeamaiswith an LD50value of 36.40 µg/adult. The essential oil also exhibited fumigant toxicity againstS. zeamaiswith an LC50value of 9.98 mg/L. The study indicates that the essential oil ofE. latifoliusaerial parts has a potential for development into a natural insecticide/fumigant for control of insects in stored grains.


2015 ◽  
Vol 14 (9) ◽  
pp. 1685-1689
Author(s):  
MP Zhao ◽  
XC Liu ◽  
QZ Liu ◽  
ZL Liu

Purpose: To investigate the chemical composition and insecticidal activity of the essential oil of the aerial parts of Ainsliaea fragrans against maize weevils (Sitophilus zeamais).Methods: The essential oil of A. fragrans aerial parts was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromaotography-mass spectrometry (GC-MS). Contact toxicity of the essential oil and its major constituents was determined by topical application against S. zeamais.Results: A total of 30 components of the essential oil were identified. The major constituents were myristicin (41.3 %), elemicine (11.9 %), cis-isosafrole (11.5 %), borneol (9.1 %) and caryophyllene (8.8 %). The essential oil of A. fragrans exhibited contact toxicity against S. zeamais with LC50 value of 50.7 μg/adult. Elemicine possessed the strongest contact toxicity (LC50 = 13.5 μg/adult) while cis-isosafrole, myristicin, caryophyllene and borneol had LC50 values of 31.2 μg/adult, 43.4 μg/adult, 57.9 μg/adult, and 98.4 μg/adult, respectively.Conclusion: The study indicates that the essential oil of A. fragrans aerial parts and its major constituents have a potential for development into natural insecticides for the control of grain storage insects.Keywords: Ainsliaea fragrans, Sitophilus zeamais, Contact toxicity, Elemicine, Essential oil, Grain storage


2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Chunxue You ◽  
Shanshan Guo ◽  
Wenjuan Zhang ◽  
Kai Yang ◽  
Zhufeng Geng ◽  
...  

The aims of this research were to determine the chemical composition and insecticidal and repellent activities of theArtemisia mongolicaessential oil againstLasioderma serricorneand to isolate active constituents from the essential oil. The essential oil ofA. mongolicawas obtained by hydrodistillation and 36 components were identified with GC-MS. Eucalyptol (39.88%), (S)-cis-verbenol (14.93%), 4-terpineol (7.20%), (−)-camphor (6.02%), andα-terpineol (4.20%) were found to be major components. With a further isolation process, five constituents obtained from the essential oil were identified as eucalyptol, verbenol, 4-terpineol, camphor, andα-terpineol. In the progress of assay, it showed thatL. serricorneadults had different sensitivities to the crude essential oil and isolated constituents. 4-Terpineol exhibited strongest contact activity againstL. serricorne, showing the LD50value of 8.62 μg/adult. Moreover, camphor andα-terpineol showed stronger fumigant activity (LC50=2.91and 3.27 mg/L air, resp.) againstL. serricornethan crude essential oil and other constituents. In addition, the essential oil, eucalyptol, verbenol, andα-terpineol showed comparable repellency againstL. serricorneadults. The results indicate that the essential oil and isolated compounds have potential to provide more efficient and safer natural insecticides or repellents for control of insects in food and Chinese medicinal materials preservation.


2020 ◽  
Vol 10 (3) ◽  
pp. 272-278
Author(s):  
Ardalan Pasdaran ◽  
Satyajit D. Sarker ◽  
Lutfun Nahar ◽  
Azadeh Hamedi

Background: The essential oil from the Acantholimon genus have been an integral part of the traditional food additive in Middle East. Most of the plants in Acantholimon genus have not been studied scientifically. The aim of this study is to investigate the chemical composition, antibacterial, insecticidal and anti-oxidant activities of three Acantholimon species including Acantholimon atropatanum, A. gilliatii and A. tragacanthium. Method: The essential oils of the aerial parts were extracted by hydrodistillation. Chemical constitutions were identified by gas chromatography- mass spectroscopy technique, also their toxicities were assessed against the two important grain products pests, Oryzeaphilus mercator and Tribolium castaneum. Antibacterial activity was assessed against the three foodborne bacteria that include Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus based on the disc diffusion assay. Free-radical-scavenging property was identified based on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Results: 2-hexahydrofarnesyl acetone was the main compound in A. gilliatii and A. tragacanthium, whilst farnesyl acetone, heptacosane and germacrene D were the principal components of A. atropatanum essential oil. These oils exhibited 40-90% mortality of O. mercator and/or T. castaneum at a dose of 12 μl/l air after 48h of exposure, and exhibited significant free-radicalscavenging property (RC50 = 3.7 × 10-3 - 8.3 × 10-3 mg/ml). The oils of A. tragacanthium and A. gilliatii showed a weaker antibacterial activity compared to A. atropatanum. Conclusion: A. atropatanum, A. gilliatii and A. tragacanthium essential oils had significant insecticidal and anti-oxidant properties. They also showed week to moderate antibacterial activity against P. aeruginosa and S. aureus.


Planta Medica ◽  
2010 ◽  
Vol 77 (08) ◽  
pp. 825-829 ◽  
Author(s):  
Antônio Venâncio ◽  
Alexandre Onofre ◽  
Amintas Lira ◽  
Péricles Alves ◽  
Arie Blank ◽  
...  

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