scholarly journals Immunomodulatory Effect of Red Onion (Allium cepaLinn) Scale Extract on Experimentally Induced Atypical Prostatic Hyperplasia in Wistar Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Ahmed A. Elberry ◽  
Shagufta Mufti ◽  
Jaudah Al-Maghrabi ◽  
Essam Abdel Sattar ◽  
Salah A. Ghareib ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Cellular Proliferation ◽  
Methanolic Extract ◽  
Histopathological Examination ◽  
Positive Control ◽  
Prostatic Hyperplasia ◽  
Saw Palmetto ◽  
Hplc Profile ◽  
Immune Enhancement ◽  
Dose Dependent

Red onion scales (ROS) contain large amounts of flavonoids that are responsible for the reported antioxidant activity, immune enhancement, and anticancer property. Atypical prostatic hyperplasia (APH) was induced in adult castrated Wistar rats by both s.c. injection of testosterone (0.5 mg/rat/day) and by smearing citral on shaved skin once every 3 days for 30 days. Saw palmetto (100 mg/kg) as a positive control and ROS suspension at doses of 75, 150, and 300 mg/kg/day were given orally every day for 30 days. All medications were started 7 days after castration and along with testosterone and citral. The HPLC profile of ROS methanolic extract displayed two major peaks identified as quercetin and quercetin-4′-β-O-D-glucoside. Histopathological examination of APH-induced prostatic rats revealed evidence of hyperplasia and inflammation with cellular proliferation and reduced apoptosis Immunohistochemistry showed increased tissue expressions of IL-6, IL-8, TNF-α, IGF-1, and clusterin, while TGF-β1 was decreased, which correlates with the presence of inflammation. Both saw palmetto and RO scale treatment have ameliorated these changes. These ameliorative effects were more evident in RO scale groups and were dose dependent. In conclusion, methanolic extract of ROS showed a protective effect against APH induced rats that may be attributed to potential anti-inflammatory and immunomodulatory effects.

scholarly journals Evaluation of Juniperus communis L. seed extract on benign prostatic hyperplasia induced in male Wistar rats

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Fatemeh Akbari ◽  
Mohammad Azadbakht ◽  
Kanu Megha ◽  
Ayat Dashti ◽  
Lale Vahedi ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Wistar Rats ◽  
Histopathological Examination ◽  
Seed Extract ◽  
Prostatic Hyperplasia ◽  
Vehicle Group ◽  
Common Disease ◽  
Therapeutic Effects ◽  
Juniperus Communis ◽  
Male Wistar Rats

Abstract Background Benign prostatic hyperplasia (BPH) is a common disease which causes various health problems for elderly men such as urinary retention, recurring urinary tract infection and bladder stones. The aim of this study is to evaluate the therapeutic effects of Juniperus communis L. seed extract (JCS) on BPH in male Wistar rats. Methods To this end, 30 rats were divided into 5 groups (N = 6): group 1 (vehicle), group 2 (disease control), group 3 (standard medicine; 10 mg/kg finasteride), and groups 4 and 5 were treated with 300 mg/kg and 600 mg/kg of the hydroalcoholic JCS seed extract, respectively. Groups 2, 3, 4 and 5 received testosterone enanthate to induce prostatic hyperplasia. At the end of experimental period (28 days), prostate glands were cut off under anesthesia. Histopathological examination was done and biochemical parameters such as Malondialdehyde, Glutathione and protein carbonyl were also measured. Their body weights were also observed during the study. At the end of the experiment, prostate weights and prostate specific antigen (PSA) levels were measured. Prostate index, inhibition prostate weight and inhibition prostate index were also calculated. Results Both histopathological examination and biochemical parameter results showed significant improvements in rats treated with finasteride and 600 mg/kg JCS extract (p < 0.01). In addition, PSA levels showed significant decrease in comparison with the disease group. But acute toxicity test indicated that using JCS extract resulted in an increase in liver enzymes (ALP, LDH, SGOT, SGPT). As a result, the extract should be used with caution. Conclusions Oral administration of JCS extract is effective on preventing testosterone-induced benign prostatic hyperplasia.

scholarly journals Effects of Melandrium firmum methanolic extract on testosterone-induced benign prostatic hyperplasia in Wistar rats

2012 ◽  
Vol 14 (2) ◽  
pp. 320-324 ◽  
Author(s):  
Mee-Young Lee ◽  
In-Sik Shin ◽  
Chang-Seob Seo ◽  
Nam-Hun Lee ◽  
Hye-Kyung Ha ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Prostatic Hyperplasia ◽  
Melandrium Firmum

scholarly journals Histostereological Restorative and Inhibitory Effects of Crude Methanolic Bark Extract of Prunus Africanus on Testosterone-Induced Benign Prostatic Hyperplasia in Wistar Rats

2021 ◽  
pp. 49-61
Author(s):  
Kanyoni J. Mwangi ◽  
Kweri J. Kariuki ◽  
Mwangi A. Warimu ◽  
Bett S. Kipyegon ◽  
Sigei C. Chepngeno ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Wistar Rats ◽  
Prostate Volume ◽  
Dose Range ◽  
Study Groups ◽  
Prostatic Hyperplasia ◽  
Bark Extract ◽  
Inhibitory Effects ◽  
Group A ◽  
Dose Dependent

Aim: To evaluate the histostereological effects of P. africanus on testosterone induced Benign Prostatic Hyperplasia (BPH) in Wistar rats. Place and Duration of Study: The study was carried out for six weeks in Jomo Kenyatta University of Agriculture and Technology. Methodology: A sample size of sixty Wistar were used as the experimental model and they were divided into two study groups of 30 rats in the restorative group and 30 rats in the inhibitory group. Each group was further categorized into 5 control and 25 experimental rats. The experimental rats were further subdivided into 5 sub-groups based on varying doses of the crude methanolic bark extract of P. africanus (0 mg, 25 mg, 50 mg, 125 mg, and 200 mg). BPH in experimental animal was induced by subcutaneous injection of testosterone propionate (7.5 mg/kg) for 10 days. Results: The findings of the study showed that the restorative group had a statistical significant (P<0.05) ((dose dependent reduction of the prostate volume, the stromal and epithelium volume was observed. While in the inhibitory group a statistical significant (P<0.05) dose-related inhibition, in the increase of the prostate volume, the stromal and epithelium volume was observed. In conclusion, the maximal restorative effect was observed to be up to 85.6% and at a dose of 200 mg, while the optimal inhibitory effects were observed to be between 66.7- 68.4% in the dose range of 100-200 mg/kg/body weight. Conclusion: Crude methanolic bark extract of P. africanus has both restorative.

Protective role of diosmin against testosterone propionate-induced prostatic hyperplasia in Wistar rats: Plausible role of oxidative stress and inflammation

2019 ◽  
Vol 39 (9) ◽  
pp. 1133-1146 ◽  
Author(s):  
A Vafa ◽  
SM Afzal ◽  
P Barnwal ◽  
S Rashid ◽  
A Shahid ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Testosterone Propionate ◽  
Specific Antigen ◽  
Protective Role ◽  
Prostatic Hyperplasia ◽  
Health Concern ◽  
Dependent Manner ◽  
Aging Men ◽  
Dose Dependent

Benign prostatic hyperplasia (BPH) is an important key health concern for aging men. Polyphenolic compounds have been found to possess important roles in the inhibition of numerous ailments that involve reactive oxygen species and inflammation. Diosmin is a citrus flavone that possesses antioxidant, anti-inflammatory, antiproliferative, and anticancer activities, so based on these properties of diosmin, we decided to evaluate its effect on testosterone propionate (TP)-induced BPH. A total of 30 Wistar rats were randomly assigned to five groups having six animals in each. This study was of 28 days in which TP (5 mg kg−1) was administered to induce BPH in the last 10 days of the study. It was found that diosmin at the doses of 20 and 40 mg kg−1 significantly reduced malondialdehyde and xanthine oxidase formation in a dose-dependent manner; however, it replenished catalase, glutathione (GSH), and GSH-dependent enzymes, that is, glutathione peroxidase, glutathione reductase, and glutathione- S-transferase significantly against TP-induced BPH. Further, immunohistochemical study showed that diosmin alleviated inflammatory markers (nuclear factor kappa-light-chain-enhancer of activated B cells, cyclooxygenase-2, and interleukin-6). It was also found that diosmin downregulated the expression of androgen receptor and decreased the prostate-specific antigen concentration dose-dependently, significantly against TP-induced BPH. Diosmin also restored histoarchitecture of the prostate in a dose-dependent manner. Findings from the present study revealed the protective role of diosmin against TP-induced BPH in Wistar rats.

scholarly journals Methanolic Extract of Ceplukan Leaf (Physalis minimaL.) Attenuates Ventricular Fibrosis through Inhibition of TNF-αin Ovariectomized Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Bayu Lestari ◽  
Nur Permatasari ◽  
Mohammad Saifur Rohman
Keyword(s):  
Heart Failure ◽  
Treatment Group ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Ovariectomized Rats ◽  
Dependent Manner ◽  
Estrogen Level ◽  
Menopausal Women ◽  
Ventricular Fibrosis ◽  
Dose Dependent

The increase of heart failure prevalence on menopausal women was correlated with the decrease of estrogen level. The aim of this study is to investigate the effects of ceplukan leaf (Physalis minimaL.), which contains phytoestrogen physalin and withanolides, on ventricular TNF-αlevel and fibrosis in ovariectomized rats. Wistar rats were divided into six groups (control (—); OVX 5: 5-week ovariectomy (OVX); OVX 9: 9-week ovariectomy; treatments I, II, and III: 9-weeks OVX + 4-week ceplukan leaf’s methanolic extract doses 500, 1500, and 2500 mg/kgBW, resp.). TNF-αlevels were measured with ELISA. Fibrosis was counted as blue colored tissues percentage using Masson’s Trichrome staining. This study showed that prolonged hypoestrogen increases ventricular fibrosis (p<0.05). Ceplukan leaf treatment also resulted in a decrease of ventricular fibrosis and TNF-αlevel in dose dependent manner compared to without treatment group (p<0.05). Furthermore, the TNF-αlevel was normalized in 2500 mg/kgBWPhysalis minimaL. (p<0.05) treatment. The reduction of fibrosis positively correlated with TNF-αlevel (p<0.05,r=0.873). Methanolic extract of ceplukan leaf decreases ventricular fibrosis through the inhibition of ventricular TNF-αlevel in ovariectomized rats.

scholarly journals A Methanolic Extract from Cucumis melo Linn. protects against Benign Prostatic Hyperplasia and is Anti-Inflammatory in Male Wistar Rats

2021 ◽  
Author(s):  
R.S. Rajasree ◽  
Sibi P. Ittiyavirah ◽  
Punnoth Poonkuzhi Naseef ◽  
Mohamed Saheer Kuruniyan ◽  
Muhammed Elayadeth-Meethal ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Cucumis Melo ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Prostatic Hyperplasia ◽  
Male Wistar Rats ◽  
Anti Inflammatory

Hepatoprotective Potential of Elsholtzia densa Against Acute and Chronic Models of Liver Damage in Wistar Rats

Drug Research ◽  
2018 ◽  
Vol 68 (10) ◽  
pp. 567-575 ◽  
Author(s):  
Ovais Zargar ◽  
Rohina Bashir ◽  
Showkat Ganie ◽  
Akbar Masood ◽  
Mohammad Zargar ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Dependent Manner ◽  
Protein Levels ◽  
Chronic Models ◽  
Dose Dependent

AbstractThe aim of the present study was to evaluate the hepatoprotective activity of methanolic extract of Elsholtzia densa against experimentally induced acute (CCl4) and chronic (paracetamol) liver injury in albino wistar rats. Activity was measured by monitoring the serum levels of ALT, ALP AST and LDH, total protein levels, bilirubin and albumin. The results of the CCl4 and paracetamol-induced liver toxicity experiments showed that the rats treated with the methanolic extract of Elsholtzia densa exhibited a significant decrease in biochemical parameters as well as the proteins, which were all elevated in the CCl4 and paracetamol group. The extract at a concentration of 300 mg/kg body wt. showed a significant decline (P≤0.05) in the levels of AST, ALT, ALP and LDH to 69.50±2.23IU/L, 60.01±2.25IU/L,46.20±2.24 IU/L and 150.21±5.68IU/L in CCl4 injected animals and 51.12±2.20 IU/L,49.15±3.25 IU/L, 44.12±2.56 IU/L and 125.15±4.45 IU/L in paracetamol-treated animals when compared to the control group. The activities of tissue antioxidants GSH, GPx, GR, GST and CAT was significantly (P≤0.05) restored in dose dependent manner in animals treated with extracts as with acute and chronic hepatotoxic models. The current study confirmed the hepatoprotective effect of methanolic extract of Elsholtzia densa against the model hepatotoxicant CCl4 and paracetamol.

scholarly journals Nephroprotective role of diosgenin in gentamicin-induced renal toxicity: biochemical, antioxidant, immunological and histopathological approach

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Prachi Mishra ◽  
Deepa Mandlik ◽  
S. Arulmozhi ◽  
Kakasaheb Mahadik
Keyword(s):  
Wistar Rats ◽  
Renal Toxicity ◽  
Histopathological Examination ◽  
Elevated Serum ◽  
Urine Volume ◽  
Kidney Tissue ◽  
Albino Rats ◽  
Dependent Manner ◽  
Dose Dependent ◽  
Nephroprotective Effect

Abstract Background Aminoglycoside antibiotics, gentamicin (GM) owns the utmost nephrotoxic potential than other antibiotics from the same category. To the other side, diosgenin (DG) showed the antioxidant and anti-inflammatory property. Results The present study was aimed to explore the nephroprotective effect of diosgenin on gentamicin-induced renal toxicity in Wistar rats. Wistar albino rats were divided into six groups (n = 6): Normal control (NC), Nephrotoxicity control (GM), DG (20 mg/kg), DG (40 mg/kg), DG (80 mg/kg), accordingly. After the treatment, the nephroprotective effects of DG were assessed by measuring serum levels of creatinine (Cr), blood urea nitrogen (BUN), total proteins (TP), albumin and urea levels. Urine volume, proteins, electrolyte levels, creatinine clearance were also evaluated in urine samples. Oxidative stress was evaluated through the measurement of antioxidant stress markers in the kidney tissue. Changes in body weight and kidney weight were also recorded along with a histopathological examination of kidney sections. For evaluation of inflammation, TNF-α and IL-1β levels were measured in the blood serum using ELISA kits. GM intoxication induced elevated serum creatinine, BUN, urea, albumin and TP levels, urine electrolytes levels, pro-inflammatory cytokines, antioxidant parameters which were found to be decreased significantly in a dose-dependent manner in rat groups received DG which was also evidenced by the histological observations. Conclusion DG showed a significant nephroprotective effect in a dose-dependent manner by ameliorating the GM induced nephrotoxicity in Wistar rats.

scholarly journals Acute and subacute toxicity study of the methanolic extract of olea europea.L leaves in Wistar rat

2021 ◽  
Vol 319 ◽  
pp. 01094
Author(s):  
Ibrahim Hinad ◽  
Youssef S’hih ◽  
Radia El Gui ◽  
Aboubaker Elhessni ◽  
Abdelhalim Mesfioui ◽  
...  
Keyword(s):  
Body Weight ◽  
Oral Administration ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Histopathological Examination ◽  
Hematological Parameters ◽  
Wistar Rat ◽  
The Body ◽  
Olive Leaves ◽  
Subacute Toxicity

Olea europea.L (olive tree) is a plant widely used in the world. Its leaves have an extensive use in traditional herbal medicine to prevent and/or treat several diseases especially in Mediterranean regions. Despite this, research into the study of its toxicity is very limited in the literature. For this, this study aims to study the acute and subacute toxicity of methanolic extract olive leaves in Wistar rats. To study acute toxicity, four dose levels (50,300,2000 and 5000 mg/kg body weight) were tested by single oral administration to Wistar rats. The subacute toxicity for 28 days was conducted by daily oral administration of methanolic extract of olive leaves at doses 1000,2000 and 3000 mg/kg in rats Wistar. After fourteen days of the administration of escalating doses, no deaths were recorded in the animals and no clinically detectable signs were observed. The results of subacute toxicity showed that the body weight of rats was not significantly changed by administration of methanolic extract of olive leaves. Monitoring of the behavior of the rats during the study showed the appearance of some manifestations of tremor, agitation and diarrhea in rats treated with the dose of 3000mg/kg. Grosse examination of the kidneys and liver revealed no changes in those organs and histopathological examination showed congestion and inflammation of the fat in rats treated with the dose of 3000mg/kg. the hematological profile showed no significant changes of the measured hematological parameters. Olive leaves should be used with care and caution since its methanolic extract caused some signs of toxicity and may affect the liver at repeated dose. however, more studies are needed to verify and clarify the toxic aspect of olea europea.L leaves.

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